1. MICHELLE RENEE TOURIGNY, PH.D.
Curriculum Vitae
Email: m_tourigny@yahoo.com
WORK HISTORY:
2014-2016 Graduate Research Assistant, Dr. Jenette Creaney
National Centre for Asbestos Related Diseases
School of Medicine and Pharmacology
University of Western Australia
Perth, Western Australia
2013 Christmas Casual Housewares Department
Myers Perth City
2006-2013 Flow Cytometry Facility Manager
Telethon Institute for Child Health Research, now Telethon Kids
Perth, Western Australia
2005 Database Administrator and Assistant in the Scientific Outreach
Office of Weill Graduate School of Medical Sciences, New York City,
USA
2004-2005 Scientific Curator, Cognia, New York, NY, USA
2003-2004 Postdoctoral Fellow, Dr. Juan Lafaille
Skirball Institute, New York Unversity Medical Center, New York
City, NY, USA
1999 – 2002 Postdoctoral Fellow, Dr. Selina Chen-Kiang
Weill Graduate School of Cornell University, New York City, NY,
USA
1992-1999 Graduate Fellow, Dr. Howard Petrie, Sloan-Kettering Institute, New York
City, NY, USA
EDUCATIONAL BACKGROUND:
Year Institution Degree and Major
1992 – 1999 Cornell University Ph.D.

2. Graduate School of Immunology
Medical Sciences
New York, NY, USA
Thesis: “Regulation of the Cell Division/Cell Death
Decision During T Cell Lymphopoiesis”
1988 – 1992 College of the Holy Cross Bachelor of Arts
Worcester, MA, USA Biology
SKILLS AND EXPERTISE:
--Proficient in the use of Macintosh and PC graphics, word processing and data analysis
software including the Microsoft Office Suite, Adobe Photoshop, GraphPad Prism, flow
cytometry software (Cellquest, FACS DIVA, Modfit, Flow Jo) and internet/email
programs such as Outlook, Internet Explorer, Mozilla Firefox and Google Chrome.
-Clinical Experience: Researching eligibility and consenting patients to clinical trials
and studies. Performing lung function tests and collecting and entering clinical data into
patient database, ensuring it is accurate and up to date. Querying the database for
research information when necessary. Ensuring that patient privacy is maintained and
that collaborating researchers receive the information needed to perform their
experiments.
-Processing of patient blood, pleural fluid and urine samples. (buffy coat, serum, plasma
and freezing down samples). Thawing patient samples and running biological assays
such as ELISAs, ELISPOTs, and flow cytometry.
-20 years of flow cytometric experience, working with Becton Dickinson
FACScan, FACS Calibur, FACS Canto, LSR2, Fortessa and cell sorters FACS Vantage
and FACS ARIA 3, including multicolor flow cytometry (up to 12 colours), DNA
content, BrDU and intracellular cytokine staining, and multicolour cell sorting. For the
years between 2006 and 2013, my work was mainly multicolour cell sorting (up to 8
colours).
-14 years of in vivo mouse experience including breeding, crossing and
maintaining colonies, working with transgenic and knockout animals (models of
immune deficiency, autoimmunity, inflammatory and cancer), isolation, purification and
antibody-based sorting (magnetic beads and flow cytometry) of thymus,
spleen, lymph node, bone marrow tissue and tumour infiltrating lymphocytes (TILs),
vaccination, BrDU and antibody (intraperitoneal, subcutaneous and tail vein injections)
production of bone marrow chimeric mice, and growth of human tumours in severely
immunodeficient mice. Frozen sectioning of mouse brain and immunofluorescent
staining.
-Cellular immune response assays such as ELISPOT, ELISA, lymphocyte proliferation

3. (tritiated thymidine and non-radioactive).
-Long term (2 week) in vitro B lymphocyte differentiation assay, starting with primary
murine cells, included growing primary cells on feeder layers (cell culture) and
production of some reagents such as secreted cytokines from transfected cells. Human
and mouse T cell expansion protocols using cytokines (IL-2) and activating magnetic
beads (CD3/28).
-Immunofluorescent microscopy on cytospun lymphocytes.
-Isolation of tail or cellular DNA, PCR and DNA agarose gels.
- SDS-PAGE gels and Western blots
RESEARCH AND TEACHING EXPERIENCE:
2014-2016 Graduate Research Assistant, Dr. Jenette Creaney
NCARD, School of Medicine and Pharmacology
Perth Australia
2014-2015 Training of Honours student in lab technique
2006-2013 Induction and training of all new flow cytometry users at Telethon
Institute.
Telethon Institute for Child Health Research
Perth, Western Australia
2003-2004 Postdoctoral Assistant, Dr. Juan Lafaille
Skirball Institute, New York Unversity Medical Center, New York
City, NY, USA
2002 Lecturer for The Cornell Institute for Biology Teachers
(CIBT) Workshops for Biology Teachers
2001-2002 Co-organizer for Weill Cornell University Immunology Program’s
Postdoctoral-invited speaker seminar series
2001-2002 Supervising mentor for Visiting Graduate Student
1999 – 2002 Postdoctoral Fellow, Dr. Selina Chen-Kiang
Weill Graduate School of Cornell University, USA
1999 - 2000 Teaching Assistant, Advanced Immunology Course
Weill Graduate School of Cornell University, USA

4. 1998 - 1999 Head Teaching Assistant, Fundamental Immunology Course
Weill Graduate School of Cornell University, USA
1992 Academic Internship, Dr. Lawrence Jung
University of Massachusetts Medical Center
Worcester, MA, USA
HONOURS AND AWARDS:
2010-2012 Recipient of Travel Fund awards from Friends of Telethon
Institute for 3 years. Funds were used to attend the Australasian
Flow Cytometry Group Annual meetings in Sydney (2010), Hobart
(2011) and Melbourne (2012)
2000 - 2002 National Research Service Award Fellow
National Cancer Institute, USA
1992 – 1995 Graduate Research Fellowship
Cornell University Graduate School of Medical Sciences, USA
1995 – 1996 Grants-in-Aid of Research Award
“Mechanism of DNA protection by Bcl-2”
Sigma Xi, The Scientific Research Society
1996 – 2015 Associate Member of Sigma Xi
The Scientific Research Honor Society, USA
1997 – 1998 Frank L. Horsfall Jr. Fellow
Memorial Sloan-Kettering Cancer Center, USA
1997 – 1998 Jack and Susan Rudin Scholar in the Biomedical Sciences
Memorial Sloan-Kettering Cancer Center, USA
MEMBERSHIP OF PROFESSIONAL ASSOCIATIONS:
1996 –present Associate Member of Sigma Xi
The Scientific Research Honor Society
2006-present Member of ISAC
International Society for the Advancement of Cytometry

5. 2008-present Member of ACS
Australasian Cytometry Society
(Formerly AFCG, Australian Flow Cytometry Group)
2012-present Member of WA Flow
Western Australian Flow Cytometry Group
BIBLIOGRAPHY:
Full Papers:
Tourigny, M. R., S. Mazel, D. Burtrum and H.T. Petrie. 1997. TCR-β Gene
Recombination: Dissociation from Cell Cycle Regulation and Developmental Progression
During T Cell Ontogeny. J. Exp. Med. 185(9):1549 – 1556.
Livak, F., M. Tourigny, D.G. Schatz and H.T. Petrie. 1999. Characterization of TCR
Gene Rearrangements During Adult Murine T Cell Development. J. Immunol. 162:2575
– 2580.
Do, R. K. G., Hatada, E., Lee, H., Tourigny, M. R., Hilbert, D., and S. Chen-Kiang. 2000
Attenuation of Apoptosis Underlies BLyS Enhancement of Humoral Immune Response.
J. Exp. Med. 192: 953-964.
Petrie, H. T., M. Tourigny, D. B. Burtrum and F. Livak. 2000. Precursor Thymocyte
Proliferation and Differentiation are Controlled by Signals Unrelated to the Pre-TCR. J.
Immunology 165: 3094-3098.
Tourigny, M. R.,*., Ursini-Siegel, J.*., Lee, H.*., Toellner, K. M., Cunningham, A. F.,
Franklin, D. S., Ely, S., Qin, X-F., Xiong, Y., MacLennan, I. C. M., and S. Chen-Kiang.
2002. CDK Inhibitor p18INK4C is Required for the Generation of Functional Plasma
Cells. Immunity 17 (2): 179-189. *These authors contributed equally to this work.
Smith M, Tourigny MR, Noakes P, Thornton CA, Tulic MK, Prescott SL 2008.
Children with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/-
) regulatory T cell function. J Allergy Clin Immunol. Jun; 121(6):1460-6. Epub 2008
May 2.
Tulic MK, Andrews D, Crook ML, Charles A, Tourigny MR, Moqbel R, Prescott SL.
2012. Changes in thymic regulatory T-cell maturation from birth to puberty: differences
in atopic children. J Allergy Clin Immunol. Jan;129(1):199-206. Epub 2011 Nov 21.
Creaney J, Ma S, Sneddon SA, Tourigny MR, Dick IM, Leon JS, Khong A, Fisher SA,
Lake RA, Lesterhuis WJ, Nowak AK, Leary S, Watson MW, Robinson, BW. 2015.
Strong spontaneous tumor neoantigen responses induced by a natural human carcinogen.
OncoImmunology. July; 4 (7):

6. Abstracts:
Tourigny, M., S. Mazel, D. Burtrum and H.T. Petrie. 1996. Regulation of the cell
division/cell death decision during T cell lymphopoiesis. Abstract for Fifteenth Summer
Symposium in Molecular Biology, Apoptosis: Death by Design. August 1-3, 1996,
Pennsylvania State University.
Tourigny, M. and H.T. Petrie. 1998. Differentiation and proliferation of thymocytes
may occur independently from pre-TCR-mediated signaling. Abstract for FASEB
Summer Research Conference “Lymphocytes and Antibodies” June 20 – 25, 1998,
Vermont Academy, Saxtons River, Vermont.
Tourigny, M., Lee, H, Ursini-Siegel, J., Qin, X-F., Xiong, Y., Franklin, D. S. and S.
Chen-Kiang. Requirement of CDK Inhibitor p18INK4C for the Generation of Plasma
Cells and B Cell Memory. Abstract for Keystone Symposia "B Cell Immunobiology and
Disease" April 17-22, 2001, Snowbird Utah.
Chen-Kiang, S., Tourigny, M.R., Ursini-Siegel, J., Lee, H., Toellner, K. M.,
Cunningham, A., F., and Ian C. M. MacLennan. Cell Cycle Control of Plasma Cell
Generation and Antibody Response. Abstract for Advances in Plasma Cell Formation
and Tumorigenesis Round Table sponsored by The Leukemia Society. March 18, 2002,
The Stanhope-Park Hyatt, New York, New York.
Tourigny, M.R., Creaney, J., Ma, S., Sneddon, S.A., Dick, I.M., Leon, J.S., and Bruce W
Robinson. Assessing tumour neoantigen recognition in mice by three different assays to
detect interferon gamma production and secretion in T lymphocytes. Abstract for
Australasian Cytometry Society Annual Conference 2015. 11th
-14th
October 2015, Perth
Parmelia Hilton Hotel, Perth, Western Australia
Publications For Which I Did The Cell Sorting:
Smith M, Tourigny MR, Noakes P, Thornton CA, Tulic MK, Prescott SL 2008. Children
2.with egg allergy have evidence of reduced neonatal CD4(+)CD25(+)CD127(lo/- )
regulatory T cell function. J Allergy Clin Immunol. Jun; 121(6):1460-6. Epub 2008 May
2.

7. Strickland DH, Wikstrom ME, Turner DJ, Holt PG. 2008. Mucosal regulatory T cells in
airway hyperresponsiveness. Chem Immunol Allergy. 94:40-7. Review.
Rate A, Upham JW, Bosco A, McKenna KL, Holt PG. 2009. Airway epithelial cells
regulate the functional phenotype of locally differentiating dendritic cells: implications for
the pathogenesis of infectious and allergic airway disease. J Immunol. Jan 1;182(1):72-83.
Yerkovich ST, Roponen M, Smith ME, McKenna K, Bosco A, Subrata LS, Mamessier E,
Wikström ME, Le Souef P, Sly PD, Holt PG, Upham JW. 2009. Allergen-enhanced
thrombomodulin (blood dendritic cell antigen 3, CD141) expression on dendritic cells is
associated with a TH2-skewed immune response. J Allergy Clin Immunol.
Jan;123(1):209-216. Epub 2008 Oct 22.
Burchell JT, Wikstrom ME, Stumbles PA, Sly PD, Turner DJ. 2009. Attenuation of
allergen-induced airway hyperresponsiveness is mediated by airway regulatory T cells.
Am J Physiol Lung Cell Mol Physiol. Mar;296(3):L307-19. Epub 2008 Nov 21.
Bosco A, McKenna KL, Firth MJ, Sly PD, Holt PG. 2009. A network modeling
approach to analysis of the Th2 memory responses underlying human atopic disease. J
Immunol. May 15;182(10):6011-21.
Subrata LS, Bizzintino J, Mamessier E, Bosco A, McKenna KL, Wikström ME,
Goldblatt J, Sly PD, Hales BJ, Thomas WR, Laing IA, LeSouëf PN, Holt PG. 2009.
Interactions between innate antiviral and atopic immunoinflammatory pathways
precipitate and sustain asthma exacerbations in children. J Immunol. Aug
15;183(4):2793-800. Epub 2009 Jul 20.
Holt PG, Strickland DH, Bosco A, Jahnsen F. 2009. Pathogenic mechanisms of allergic
inflammation: atopic asthma as a paradigm. Adv Immunol. 104:51-113. Epub 2009 Dec
1. Review.
Bertram CM, Hawes SM, Egli S, Peh SL, Dottori M, Kees UR, Dallas PB. 2010. Stem
Cells Dev. 2010. Effective adenovirus-mediated gene transfer into neural stem cells
derived from human embryonic stem cells. Stem Cells Dev. Apr;19(4):569-78.

8. Wikstrom ME, Batanero E, Judd SR, Wiqvist K, Holt PG, Stumbles PA. 2010. Lung
homing T-cell generation is dependent on strength and timing of antigen delivery to
lymph nodes. Immunol Cell Biol. Aug;88(6):658-66. Epub 2010 Mar 2.
McGlade, JP, Strickland DH, Lambert MJM, Gorman S, Thomas JA, Judge MA,
Burchell J, Zosky G, Hart PH. 2010. UV inhibits allergic airways disease in mice by
reducing effector CD4+ T cells. Clinical and Experimental Allergy 40:772-85
Gorman S, Judge MA, Burchell JT, Turner DJ, Hart PH. 2010. 1,25-dihydroxyvitamin
D3 enhances the ability of transferred CD4+CD25+ cells to modulate T helper type 2-
driven asthmatic responses. Immunology 130:181-92
Gorman S, Judge MA, Hart PH. 2010. Topical 1,25-dihydroxyvitamin D3 subverts the
priming ability of draining lymph node dendritic cells. Immunology 131:415-25
Stumbles PA, Strickland DH, Wikstrom ME, Thomas JA, von Garnier C and Holt
PG. 2010. Identification and isolation of rodent respiratory tract dendritic cells. In:
Dendritic Cell Protocols, Naik, S (ed.) Humana Press. 595:249-63.
Gorman S, McGlade JP, Lambert MJM, Strickland DH, Thomas JA, Hart PH. 2010. UV
exposure and protection against allergic airways disease. Photochemical and
Photobiology Sciences. 9: 571-7.
Strickland DH, McGlade JP, Lambert MJM, Gorman S, Thomas JA, Judge MA, et al.
2010. UV inhibits allergic airways disease in mice by reducing effector CD4+ T cells.
Clin Exp Allergy 40: 772-785.
Holt PG, Strickland DH. 2010. Interactions between innate and adaptive immunity in
asthma pathogenesis: new perspectives from studies on acute exacerbations. J Allergy
Clin Immunol 125: 963-972.
Strickland DH, Upham JW, Holt PG. 2010. Epithelial-dendritic cell interactions in
allergic disorders. Curr Opin Immunol. Dec;22(6):789-94. Epub 2010 Nov 23. Review.
Strickland DH, Judd S, Thomas JA, Larcombe AN, Sly PD, Holt PG. 2011. Boosting
airway T-regulatory cells by gastrointestinal stimulation as a strategy for asthma control.
Mucosal Immunol. Jan;4(1):43-52. Epub 2010 Jul 28.

9. Genovesi LA, Carter KW, Gottardo NG, Giles KM, Dallas PB. 2011. Integrated analysis
of miRNA and mRNA expression in childhood medulloblastoma compared with neural
stem cells. PLoS One. 6(9):e23935. Epub 2011 Sep 9.
Fear VS, Burchell JT, Lai SP, Wikstrom ME, Blank F, von Garnier C, Turner DJ, Sly
PD, Holt PG, Strickland DS, Stumbles PA. 2011. Restricted aeroallergen access to
airway mucosal dendritic cells in vivo limits allergen-specific CD4+ T cell proliferation
during the induction of inhalation tolerance. J Immunol. Nov 1;187(9):4561-70. Epub
2011 Sep 19.
Tulic MK, Andrews D, Crook ML, Charles A, Tourigny MR, Moqbel R, Prescott SL.
2012. Changes in thymic regulatory T-cell maturation from birth to puberty: differences
in atopic children. J Allergy Clin Immunol. Jan;129(1):199-206. Epub 2011 Nov 21.
Lisciandro JG, Prescott SL, Nadal-Sims MG, Devitt CJ, Pomat W, Siba PM, Holt PG,
Strickland D, van den Biggelaar AH. 2012. Comparison of neonatal T regulatory cell
function in Papua New Guinean and Australian newborns. Pediatr Allergy Immunol.
Mar;23(2):173-80. Epub 2011 Dec 23.
Strickland DH, Thomas JA, Mok D, Blank F, McKenna KL, Larcombe AN, Sly PD, Holt
PG. 2012. Defective aeroallergen surveillance by airway mucosal dendritic cells as a
determinant of risk for persistent airways hyper-responsiveness in experimental asthma.
Mucosal Immunol. May;5(3):332-41. Epub 2012 Feb 22.
Genovesi LA, Anderson D, Carter KW, Giles KM, Dallas PB. 2012. Identification of
suitable endogenous control genes for microRNA expression profiling of childhood
medulloblastoma and human neural stem cells.
BMC Res Notes. Sep 14;5:507.
Rate A, Bosco A, McKenna KL, Holt PG, Upham JW. 2012. Airway epithelial cells
condition dendritic cells to express multiple immune surveillance genes. PLoS One.
2012;7(9):e44941. Epub 2012 Sep 11.
Lisciandro JG, Prescott SL, Nadal-Sims MG, Devitt CJ, Richmond PC, Pomat W, Siba
PM, Holt PG, Strickland DH, van den Biggelaar AH 2012. Neonatal antigen-presenting
cells are functionally more quiescent in children born under traditional compared with
modern environmental conditions. J Allergy Clin Immunol. Nov;130(5):1167-1174.
Epub 2012 Jul 19.

10. Connective tissue growth factor is expressed in bone marrow stromal cells and promotes
interleukin-7-dependent B lymphopoiesis. Cheung LC, Strickland DH, Howlett M, Ford
J, Charles AK, Lyons KM, Brigstock DR, Goldschmeding R, Cole CH, Alexander WS,
Kees UR. Haematologica. 2014 Jul;99(7):1149-56. doi: 10.3324/haematol.2013.102327.
Epub 2014 Apr 11.