Resime

1. TIBOR FARKAS, DVM, PhD, MBA
2540 Coveyrun Court, Cincinnati, OH 45230 (513) 686 0154 farzheng@gmail.com
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SUMMARY Scientist with multidisciplinary education, work experience, and unique understanding of
diverse cultural and interdisciplinary settings. Over 20 years of extensive microbiology/virology
laboratory research experience in academic settings including epidemiology, molecular microbiology,
diagnostics, vaccine and animal model development. Proven leadership, project management,
experimental design, grant writing, organizational, managerial, regulatory, troubleshooting, mentoring,
teaching and communication skills. High productivity, adaptability, high personal and ethical standards,
motivated as an individual or as a member of a team.
APPOINTMENTS
Researcher – 2001 - present – Cincinnati Children’s Hospital Medical Center (CCHMC), Division of
Infectious Diseases (Research Associate – 2014-2015; Instructor – 2001-2006, Assistant Professor –
2007-2014)
-as a research associate at the Laboratory for Specialized Clinical Studies from 2014 to 2015, developed a
multiplex real time RT-PCR assay for the simultaneous detection of GI, GII, and GIV noroviruses for the first time.
- significantly improved the sensitivity and cut the cost by 50% of a real time RT-PCR assay developed by the
CDC for the detection of enterovirus D68.
-demonstrated natural human norovirus infections of captive rhesus macaques.
-as a faculty member at the Division of Infectious Diseases from 2001 to 2014, led an independent laboratory
involved in human and animal enteric virus research. Discovered several novel viral enteric pathogens including
the Tulane virus and other recoviruses, Galliviruses, mouse astroviruses in laboratory and wild mice, noroviruses
in wild mice, novel human norovirus and sapovirus strains. Developed the recovirus/rhesus macaque cell culture
and animal model for studying the uncultivable human noroviruses and extended basic understanding on
calicivirus replication, infection, virus/host interactions. Developed several diagnostic assays for the molecular and
serological detection of different enteric viruses. Participated in large epidemiological cohort and outbreak studies
of noroviruses, including outbreaks on large Navy ships or among military personnel during combat operations.
Was involved in teaching activities of the division. Served as PI or Co-PI on several NIH grants with budgets from
$250,000 to $8,000,000.
Research Scientist – 1989 - 1994 – Veterinary Medical Research Institute, The Hungarian Academy
of Sciences, Budapest, Hungary
-worked as an avian virologist, studying infectious chicken anemia, Marek’s disease, reticuloendotheliosis and
lymphoid leucosis. Described chicken anemia first time in Hungary and isolated the first Hungarian CAV for which
in 1993 received the Babolna-Arbor Acres Award in Avian Virology.
TECHNICAL SKILLS
Virology Immunology/Biochemistry Molecular Biology
Clinical virology (sample collection, Immunogenicity assays Plasmid preparation
evaluation of clinical disease) ELISA (antigen and antibody detection) DNA/RNA isolation
Pathogen discovery/characterization SDS-PAGE/Western blot PCR/RT-PCR (quantitative,
Cell culture adaptation HPLC real time)
Cell culture (primary, insect, mammalian, Polyclonal/monoclonal antibody Cloning
adherent, suspension) Protein expression/purification Sequencing
Virus neutralization (insect cell, bacterial, yeast) Phylogeny
Plaque assays ELISPOT Transfection
Serotyping Luminex Mutagenesis
Virus like particle preparation In vitro transcription
Microscopy (EM, Confocal) Computer Reverse genetics
VOPBA, MALDI-TOF MS Office Suite
Animal models (primate, lab animals) GraphPad Prism Quality Control
Animal handling (small and large) Powerpoint/Photoshop GCP (Clinical)

2. Biosafety Level 2 and 3 Outlook/Peoplesoft GLP (Laboratory)
MEGA/OMIGA/GenDOC/ GCP (Communication)
Languages Sequencher/Chromas/RNAfold SOP, IRB, BioSafe
English, Hungarian (fluent) Informed Consent
Japanese, German (intermediate/novice)
Leadership/administration
Supervision, interviewing, hiring, budgeting, performance evaluation, reporting, grant writing.
PUBLICATIONS
Authored or co-authored 53 manuscripts in peer-reviewed journals, 65 abstracts in national and
international conferences and 5 book chapters.
TRAINING
Visiting Scientist – 1993 - 1994 – Institute for Poultry Diseases, Freie Universitat, Berlin, Germany
Postdoctoral fellow – 1998 - 2001 – Center for Pediatric Research, Children’s Hospital of The King’s
Daughters and Eastern Virginia Medical School, Norfolk, VA
AWARDS AND HONORS
Babolna-Arbor Acres Award in Avian Virology – 1993
DAAD Fellowship, Germany – 1993-1994
Monbusho Fellowship, Japan – 1994-1998
Travel Award, American Society for Virology – 1999
US citizenship – 2010
Convenor of the “Caliciviruses and Astroviruses” workshop at the 33rd Annual Meeting of the American
Society for Virology, Fort Collins, CO – 2014
MEMBERSHIPS IN PROFESSIONAL ORGANIZATIONS
Hungarian Society for Microbiology – 1989 - 1994
Japanese Veterinary Association – 1994 - 1998
American Society for Microbiology – 2010-2014
American Society for Virology – 1998-present
SERVICE
Invited Reviewer: Journal of Virology, Journal of Infectious Diseases, Journal of Clinical Microbiology,
Applied and Environmental Microbiology, Clinical and Vaccine Immunology, Emerging Infectious
Diseases, Food and Environmental Virology, Virology Journal, PLOS One.
EDUCATION
Masters of Business Administration (GPA: 3.67/4) – 2008 – University of Cincinnati, College of
Business
Ph.D. in Veterinary Microbiology – 1998 – United Graduate School of Veterinary Sciences,
Yamaguchi University, Yamaguchi, Japan
Doctor of Veterinary Medicine (cum laude) – 1988 – University of Veterinary Medicine, Budapest,
Hungary (Today: Szent Istvan University, College of Veterinary Medicine)

3. Appendix
Major accomplishments
1. First report and isolation of chicken infectious anemia virus in Hungry.
Farkas et al., (1992). Isolation of chicken anaemia virus from broiler chickens. Acta Veterinaria Hungarica. 40. 207-223.
2. Design and evaluation of a universal primer pair that led to the discovery of several novel caliciviruses.
Jiang et al., (1999) Design and evaluation of a primer pair that detects both Norwalk- and Sapporo-like caliciviruses by RT-PCR. Journal
of Virological Methods. 83. 145-154.
Farkas et al., (2004). Genetic diversity among Sapoviruses. Archives of Virology, 149 (7):1309-23.
3. Description and characterization of norovirus outbreaks on large Navy ships and in other military settings.
Thornton et al., (2002); Detection of Norwalk-like Virus Infection Aboard Two U.S. Navy Ships. Military Medicine 167: 826-830.
Farkas et al., (2003).Homologous vs. heterologous immune responses to Norwalk-like viruses among crewmembers following acute
gastroenteritis outbreaks on two US Navy vessels. Journal of Infectious Diseases, 187 (2): 187-193.
Thornton et al. (2005). Gastroenteritis in Unites States Marines during Operation Freedom. Clin Infect Dis. 40(4):519-25.
4. Establishing sapovirus genetic diversity, including the existence of 5 genogroups.
Farkas et al., (2004). Genetic diversity among Sapoviruses. Archives of Virology, 149 (7):1309-23.
5. Description of the diversity of human norovirus histo-blood group antigen binding.
Huang et al., (2003). Noroviruses bind to human ABO, Lewis and secretor histo-blood group antigens: Identification of four distinct strain-
specific patterns. Journal of Infectious Diseases, 188(1): 19-31.
Huang et al., (2005) Norovirus and histo-blood group antigens: demonstration of a wide spectrum of strain specificity and classification of
two binding groups among multiple binding patterns. J Virol. 79(11): 6714-22.
Tan M et al., (2003). Mutations within the P2 domain of norovirus capsid affect binding to human histo-blood group antigens: evidence for
a binding pocket. Journal of Virology, 77 (23):12562-71.
Tan et al., (2008). Elucidation of strain-specific interaction of a GII-4 norovirus with HBGA receptors by site directed mutagenesis study.
Virology. 379 (2):324-34.)
6. Discovery and tissue culture adaptation of the Tulane virus and other recoviruses and development of the first
reverse genetic system for a non-human primate calicivirus.
Farkas et al., (2008). Characterization of a rhesus monkey calicivirus representing a new genus of Caliciviridae. J Virol. 82(11):5408-16.
Wei et al., (2008). Recovery of Infectious Virus by Transfection of in vitro Generated RNA from Tulane Virus cDNA. J Virol. 82
(22):11429-36.
7. Developing the recovirus/non-human primate cell culture and animal model for the uncultivable human
noroviruses.
Farkas et al., (2010). Genetic diversity and histo-blood group antigen interactions of rhesus enteric caliciviruses. J Virol.. 84., No. 17,
8617-8625.
Sestak et al., (2012). Experimental Inoculation of Juvenile Rhesus Macaques with Primate Enteric Caliciviruses. PLoS One.
2012;7(5):e37973.
Farkas et al.,(2014). Relationship between genotypes and serotypes of genogroup 1 recoviruses: A model for human norovirus antigenic
diversity.
Farkas T. (2014) Rhesus enteric calicivirus surrogate model for human norovirus gastroenteritis. JGV. 2014 Dec 12. pii: jgv.0.000020.
doi: 10.1099/jgv.0.000020. [Epub ahead of print]
8. Demonstrating norovirus and recovirus interspecies/zoonotic transmission between human and non-human
primate populations.
Farkas et al., (2010). Detection of norovirus-, sapovirus- and rhesus enteric calicivirus-specific antibodies in captive juvenile macaques. J
Gen Virol. 91(Pt 3):734-8.
Farkas et al., (2010). Genetic diversity and histo-blood group antigen interactions of rhesus enteric caliciviruses. J Virol.. 84., No. 17,
8617-8625.
Farkas et al., (2014). Recovirus neutralizing antibody prevalence in human serum samples. J Clin Microbiol. 52(8):3088-90.
Farkas et al., Natural norovirus infections of non-human primates. (Submitted).
9. Discovery of Galliviruses.
Farkas et al., (2011). Detection of novel picornaviruses in chickens and turkeys. Virus Genes. (2):262-72.
10. Discovery of novel astroviruses and noroviruses in laboratory and wild mice
Farkas et al., (2012). Molecular detection of novel astroviruses in wild and laboratory mice. Virus Genes. 2012 Dec;45(3):518-25.
Farkas et al., (2012). Molecular detection of murine noroviruses in laboratory and wild mice. Vet Microbiol. 2012 Dec 7;160 (3-4):463-7.