Culture of bacteria is having trouble dividing. Choose from the list of genes to show which of them might have a mutation responsible for the following malfunctions. Choose the best gene for each malfunction. All will not be used. ftsZ minC zipA xerD DNA pol III dnaA TOPOIV Malfunction 1-Two daughter cells result, but one has two copies of the chromosome concatenated into a big circle. Malfunction 2-Replication never starts. Malfunction 3-Cell divides at the poles Malfunction 4-DNA replication has slowed such that it is not finished with the whole genome by the time cell is trying to septate. Solution Malfunction 1-Two daughter cells result, but one has two copies of the chromosome concatenated into a big circle. -TOPO IV Introduces single-stranded break into concatenated chromosomes to release them from each other. And if it is mutated then concatenated chromosomes are not separated and may be one cell has two copies of chromosome into big circle. Malfunction 2-Replication never starts. -DnaA is a protein that activates initiation of DNA replication in bacteria. So if this protein is mutated then replication is not initiated I.e. never starts. Malfunction 3-Cell divides at the poles. -MinC is known to dimerize and functions as an FtsZ protein inhibitor(It forms a ring at the site of constriction). And if MinC is mutated then cell divides at poles. Malfunction 4-DNA replication has slowed such that it is not finished with the whole genome by the time cell is trying to septate. -DNA pol III is the main enzyme that adds nucleotides in the 5 to 3 direction I.e It helps in strand elongation. But this is mutated then DNA replication is not appropriate..