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You identify four mutant strains that cannot synthesize Compound E. Each strain contains a
single mutation. You test the ability of each of these mutants to grow when a specific compound
(known to be an intermediate in the synthesis of Compound E) is added to the minimal growth
medium. In the table below, the ability to grow is represented by "+" and the inability to grow
is represented by "-". Propose a biosynthetic pathway for the synthesis of Compound E.
Compounds that are added to the growth media a. Draw the biosynthetic pathway for Compound
E, indicating the order in which the intermediates are generated and which step is affected by
each mutant. b. You isolate a fifth mutant and show that it complements all five of the mutants
described above. When tested for growth on compounds A-E, it shows a pattern identical to
mutant 2. How could you explain this result?
Solution
Answer:
The biosynthetic pathway for compound E starts with compound A. Compound A produce
compound D. From compound D, compound C is obtained and compound D is the immediate
precursor for compound E.
In the second part of the question a fifth mutant has been introduced. Which is having same
scenario as mutant 2. It indicates both mutation effects the same loci or loci of same function in
the individual.

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You identify four mutant strains that cannot synthesize Compound E. E.pdf

  • 1. You identify four mutant strains that cannot synthesize Compound E. Each strain contains a single mutation. You test the ability of each of these mutants to grow when a specific compound (known to be an intermediate in the synthesis of Compound E) is added to the minimal growth medium. In the table below, the ability to grow is represented by "+" and the inability to grow is represented by "-". Propose a biosynthetic pathway for the synthesis of Compound E. Compounds that are added to the growth media a. Draw the biosynthetic pathway for Compound E, indicating the order in which the intermediates are generated and which step is affected by each mutant. b. You isolate a fifth mutant and show that it complements all five of the mutants described above. When tested for growth on compounds A-E, it shows a pattern identical to mutant 2. How could you explain this result? Solution Answer: The biosynthetic pathway for compound E starts with compound A. Compound A produce compound D. From compound D, compound C is obtained and compound D is the immediate precursor for compound E. In the second part of the question a fifth mutant has been introduced. Which is having same scenario as mutant 2. It indicates both mutation effects the same loci or loci of same function in the individual.