Covid-19, commonly known as Coronavirus, is a single-stranded positive-sense RNA virus. It is a known fact that RNA-duplex and RNA-DNA duplex is thermodynamically more stable than ds-DNA which in turn is more stable than ss-RNA i.e. it requires more harsh conditions (Like higher temperature) to denature ds-RNA than ds-DNA. So, injecting a modified anti-sense RNA would effectively arrest RNA proliferation by forming a near-neutral duplex (i.e. this Duplex can't be proofread stopping the retrosynthesis) in a Corona-affected patient, which is the key idea of my project.

1. Complimentary Silencers

2. RT-PCR (amplifies cDNA and RNA)
More about RNA amplification:
https://www.future-science.com/doi/pdf/10.2144/03342mt04
https://sci-hub.tw/https://doi.org/10.1016/S0167-
4781(02)00280-4
Make antisense RNA
Add histone-acetyltransferase and DNA methyltransferase to silence cDNA. DNA methyltransferase
also,methylates RNA, preventing itself from self-ligated and hindering unwanted peptide synthesis

3. Add GFP tag (green fluorescent protein) to cDNA and our synthesized
antisense RNA [Ref. :Trends Cell Biol. 2010 Jul; 20(7): 380–390.
doi: 10.1016/j.tcb.2010.03.006]
Introduce these cDNA and antisense-RNA to empty capsid of viral
vector. RNA to RNA-viral vector and cDNA to DNA-viral vector
(like adenovirus)
Inject these viral vector along with catalytic amount of dicer (to digest ds-RNA) and RnaseH (to digest cDNA-RNA
duplex) through automated microinjection

4. The drug is delivered through microneedle drug patch that analyzes fluorescence intensity at real
time and delivers drug automatically. Thus serving as a real-time diagnostic tool as well as a
therapeutic tool.

5. What happens after injection ?
 The viral vector inject antisense RNA and cDNA
 RNA-duplex and RNA-DNA duplex would be formed, silencing covid-19 RNA
 RNAse h (https://en.wikipedia.org/wiki/Ribonuclease_H) will digest viral RNA of
DNA-RNA duplex and Dicer enzyme will digest ds-RNA
 Green fluorescent protein tag will be digest in presence of viral RNA
 In absence of viral RNA, green fluorescence will be shown, triggering the laser-
assisted LDR, stopping the injection serving as a dianostic tool as well
 Also, it is temperature controlled, it will stop reaction and injection for any drastic
fluctuation in temperature that might incur star activity.

7. Labelled diagram of the interior of
automated microneedle patch

8. SIMPLIFIED CIRCUIT DIAGRAM OF AUTOMATED DRUG-ADMINISTERER

9. PCB for automated mass production

10. AUTOCAD designed automated microneedles

11. Printed Circuit Board of automated
microinjection with real time analysis

12. Severe acute respiratory syndrome coronavirus 2 isolate
Wuhan-Hu-1, complete genome
Reference : https://www.ncbi.nlm.nih.gov/nuccore/NC_045512.2?report=fasta

13. Drug efficiency analysis by simple genetic
algorithm in an infected cell
In a simple cell infected with 10,000 COVID-19 viral RNA strain, all of them obliterated by our drug
(50% binding) under 247 ms proving our drug potential cure against corona-virus

14. Modified computational analysis
The length of Covid-19 RNA strand is 29882 bp and DICER enzyme requires atleast 500 bp (bp=
base pairs) so a minimum overlap of 1.67% (=500/29882 X 100) required for enzyme to work. It
took about 501time-units to obliterate 10000 viral RNA in an infected cell.

15. Single site cutter

16. ACKNOWDGEMENT
Project Coordinator
 Swastik Biswas (email : swastik.biswas@students.iiserpune.ac.in )
Designer and Engineer
 Shubham Roy (email : shubhamroy036@gmail.com )
 Pritam Sil (email : pritamsil1998@gmail.com )
 Satadru Banerjee (email: satadru.banerjee20@gmail.com )
 Sayan Ray (email : sayanrayz1998@gmail.com )
 Riddhi Manna (email: riddhi.manna@students.iiserpune.ac.in)