Targeted and Genome Wide DNA methylation analysis. EPIC and oxEPIC array.

1. Susanna Lovell
slo@diagenode.com
Find out about our wet lab
analysis service options for
DNA Epigenomic Profiling
including our newest
offering: oxEPIC Array
to detect 5-hmC in DNA.
DNA Methylation and
Hyroxymethylation
Wet Lab Analysis
EPIC Array
Analysis
oxEPIC Array
Analysis
Methyl and Hydroxymethyl DNA Analysis

2. START UP as a local biotech
2003
R&D Labs 2004
story
Epigenomics Profiling
Service
2
2015
NXT-Dx
acquistion
Innovating Epigenetics Solutions
2020scATAC-seq
Megaruptor
3-100kb
Bioruptor
100-1000bp
Didier Allaer CEO
Susanna Lovell
slo@diagenode.com
Diagenode was founded in 2003 by Didier Allaer in Belgium
with a focus on diagnostics and epigenetics.
I am Susanna, your local contact for the UK and Ireland

3. Wet Lab Epigenomics Service Options
Susanna Lovell
slo@diagenode.com
Since 2015 we have expanded our portfolio of wet lab services and
bioinformatics analyses which now includes data mining and machine
learning to develop algorithms from existing data sets.

4. 12 yrs' Experience in Epigenetics
Susanna Lovell
slo@diagenode.com
Our growing
Service team of
PhD Epigeneticists
Our experienced Service Team are happy to discuss project ideas.
Arrange a call and to discuss how to get the most out of your
experimental design for fast results with maximum impact.

5. DNA Methylation Analysis Options
Susanna Lovell
slo@diagenode.com
• Custom Capture Probes
• Bisulfite Amplicon Sequencing
• Pyrosequencing
Single Nucleotide Resolution
• WGBS
• RRBS
• EPIC
• oxEPIC
• MeDIP-seq
• hMeDIP-seq
100-500 bp Resolution
Gene Specific Targets
We have options
for genome wide
and gene specific
options for all
species.
Our new oxEPIC
array is a cost
effective way to
detect 5-hmC
across the human
genome.
Genome Wide Targets

6. Whole Genome Bisulfite Sequencing: Gold Standard – All species, complete coverage
Reduced Representation Bisulfite Seq: 4 million CpG coverage
Applicable for all vertebrates
Significantly reduced sequencing
costs compared to WGBS
Reduced Representation BS & WGBS
Susanna Lovell
slo@diagenode.com
Bisulfite Conversion Sequencing
We can offer both WGBS & RRBS and are happy to discuss the
advantages of both options.
Reduced Representation
Bisulfite Sequencing
• Enriches for CpG islands using an
MspI enzyme
• This allows detection of > 80% of
CpG sites while requiring
< 5% of the sequencing.
• CpG island methylation is
conserved for all mammalian and
vertebrate species.

7. 5-mC and 5-hmC Methylation
Susanna Lovell
slo@diagenode.com
Hydroxymethylated DNA can be detected using antibodies in our
immune precipitation based hMeDIP and also at single nucleotide
resolution, using our new oxidative bisulphite array, oxEPIC.

8. EPIC & oxEPIC for 5-hmC analysis
Susanna Lovell
slo@diagenode.com
Oxidation of 5hmc
gives 5-formyl C.
Bisulfite converts
5fC T.
Array analysis
followed by
subtraction of the
oxBS signal allows
distinction of all 3
forms of Cytosine.

9. Targeted Methylation Analysis
Susanna Lovell
slo@diagenode.com
Arrange a call
to discuss the
options with
our experts
and find out
which
technique is
best for your
experimental
requirements.
Bisulfite Amplicon Sequencing (BSAS)
• BSAS: PCR based bisulfite amplicon sequencing is suitable for targets up to
250 bp in all species
• Custom capture probes: Can be used for targets from 200 Kb to 24 Mb.
Suitable for use with all species.
• Pyrosequencing: Suitable for human, mouse, rat and monkey samples.
Targets up to 80 bp can be analysed from samples of 100-200ng

10. MeDIP & hMeDIP
Susanna Lovell
slo@diagenode.com
MeDIP and hMeDIP are
suitable for all species
and offer bias free
analysis of the whole
genome with 200-300 bp
resolution.
Allows distinction
between methylated &
hydroxymethylated DNA.
DNA Immunoprecipitation
Antibodies are
available to
5-mC for MeDIP
and 5-hmC for
hMeDIP.

11. Thank you for your attention!
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