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Topic: Cell Transport
Prepared By:
Nikhil A. Borode
Assistant Professor
Department of Zoology
Traffic Across Membranes
Selective permeability depends on solubility
characteristics of the lipid bilayer, and presence of
specific integral proteins
Permeability of the Lipid Bilayer
NONPOLAR Molecules
• Dissolve in membranes, cross
with ease
• Smaller of 2 will cross faster
POLAR Molecules
• Small, polar, uncharged
molecules can slip through
• Larger, polar, uncharged will not
get through easily (glucose)
• All ions have trouble getting
through hydrophobic layer
Transport Proteins
• Hydrophilic Substances like ions and moderately sized polar
molecules can avoid going through the hydrophobic core of the
membrane by going through transport proteins
• Integral membrane proteins that transport specific ions or molecules
across the membrane. They may provide a hydrophilic tunnel or may
bind to, and physically carry a substance across. These are specific
Some Functions of Membrane Proteins
Proteins Facilitate Passive Transport
• Facilitated Diffusion: specific transport proteins help solutes
diffuse across membrane
• Is passive transport (down conc. Gradient)
• Helps many polar molecules/ions get through the lipid bilayer
• SPECIFIC-Have binding site like active site
• Can be saturated with solute: rate limited
• Can be due to conformational changes
• Can be gated channels
ACTIVE TRANSPORT
• Endergonic process by which a transport protein pumps a
molecule across a membrane AGAINST its concentration
gradient.
• These maintain concentration gradients across membranes
• Use ATP as energy source.
• Ex. Sodium-Potassium Pump
Passive/Active Transport
Facilitated Transport
MEMBRANE POTENTIAL
• Voltage across membranes happens when anions/cations are
unequally distributed across cell membranes
• Potential ranges from -50 to -200 mv
• Negative sign indicates the inside of the cell is – charged.
• Affects traffic of charged subs. across membrane, favors
diffusion of anions out, cations in.
Passive Transport of Ions Depends On:
• Concentration gradient of the Ion
• Effect of the membrane potential of the ion
• The Electrochemical Gradient is the diffusion gradient created from
the combined effects of both of the above

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Cell transport.pptx

  • 1. Topic: Cell Transport Prepared By: Nikhil A. Borode Assistant Professor Department of Zoology
  • 2. Traffic Across Membranes Selective permeability depends on solubility characteristics of the lipid bilayer, and presence of specific integral proteins
  • 3. Permeability of the Lipid Bilayer NONPOLAR Molecules • Dissolve in membranes, cross with ease • Smaller of 2 will cross faster POLAR Molecules • Small, polar, uncharged molecules can slip through • Larger, polar, uncharged will not get through easily (glucose) • All ions have trouble getting through hydrophobic layer
  • 4. Transport Proteins • Hydrophilic Substances like ions and moderately sized polar molecules can avoid going through the hydrophobic core of the membrane by going through transport proteins • Integral membrane proteins that transport specific ions or molecules across the membrane. They may provide a hydrophilic tunnel or may bind to, and physically carry a substance across. These are specific
  • 5. Some Functions of Membrane Proteins
  • 6. Proteins Facilitate Passive Transport • Facilitated Diffusion: specific transport proteins help solutes diffuse across membrane • Is passive transport (down conc. Gradient) • Helps many polar molecules/ions get through the lipid bilayer • SPECIFIC-Have binding site like active site • Can be saturated with solute: rate limited • Can be due to conformational changes • Can be gated channels
  • 7. ACTIVE TRANSPORT • Endergonic process by which a transport protein pumps a molecule across a membrane AGAINST its concentration gradient. • These maintain concentration gradients across membranes • Use ATP as energy source. • Ex. Sodium-Potassium Pump
  • 10. MEMBRANE POTENTIAL • Voltage across membranes happens when anions/cations are unequally distributed across cell membranes • Potential ranges from -50 to -200 mv • Negative sign indicates the inside of the cell is – charged. • Affects traffic of charged subs. across membrane, favors diffusion of anions out, cations in.
  • 11. Passive Transport of Ions Depends On: • Concentration gradient of the Ion • Effect of the membrane potential of the ion • The Electrochemical Gradient is the diffusion gradient created from the combined effects of both of the above