2. Cell Boundaries Chapter 6

3. What are boundaries? We have borders surrounding countries We have boundaries on a playing field Why are borders important?

4. What are the borders of a cell? Cell membrane Thin, flexible barrier Cell wall Plant, algae, fungi, prokaryotes Strong supporting layer

5. Cell Membrane What does it do for cell? Controls what goes in and out Regulates molecules moving from one liquid side of the cell to the other liquid side of the cell Protects Supports

6. Cell Membrane Lipid bilayer What are lipids? What does bi- mean? What’s a layer? A cell membrane is made of two layers of lipid molecules

7. Cell membrane Phospholipids bilayer Made of a negatively charged phosphate “head” PO43- Attracts water because the phosphate is charged (-) Water is a polar , slightly positive ends and slightly negative ends Attached to the phosphate group are 2 fatty acid chains Hydrophobic= don’t like water So the inside of the cell membrane doesn’t let water in but the outside allows cells to be dissolved in aqueous environments

9. Other things in the membrane… Proteins embedded in lipid bilayer Carbohydrates attached to proteins So many different molecules in membrane, we call it a “mosaic” of different molecules

11. Proteins Proteins help things get across membrane Kinda like a pump

12. Carbohydrates… Chemical identification cards “ID” card of cell Helps individual cells id each other

14. Cell Walls Outside cell membrane (does NOT replace membrane!!!) Plants, algae, fungi, prokaryotes Have pores to allow things in Function: SUPPORT and PROTECT

16. Cell walls made of… Protein and carbs Plant cell walls made up of CELLULOSE This is a tough carbohydrate Wood and paper

17. A biological membrane Cells exist in liquid environments Things need to get in and out of cell Different ways to do this…. The Cell Membrane

18. Concentration (conc.) Solution Mix of 2 or more substances Solutes Substance dissolved in solution Concentration Molarity (M) is # of Moles of a substance per liter of solution 6.02 x 1023 “small things” (molecules, atoms, ions, electrons, ions, etc) Moles is a unit for measuring EXTREMELY small things is mass of SOLUTE in a given volume of solution (g/L) What is the concentration of 12 grams of salt dissolved in 3 liters of water? 12g/3L= 4g/L

19. Diffusion Particles constantly move Collide randomly Spread out randomly Diffusion is moving from area of HIGH conc. to area of LOW conc. This is what we call the CONCENTRATION GRADIENT

21. Equilibrium When the conc. Of a system is the same throughout (same conc. on both sides)

22. What happens when we reach equilibrium? Particles continue moving across membrane but in both directions!***No more changes in concentration

23. If things can cross a membrane we call the membrane PERMEABLE If things canNOT cross a membrane we call the membrane IMPERMEABLE

24. Biological membranes are… SEMI-PERMEABLE A.K.A. Selectively permeable Cell membranes are picky

25. What’s this have to do with cells? Cell have liquid inside and are found in liquid environment We have substances (solutes) inside and outside cell Unequal concentrations means we get DIFFUSION!!! B/c diffusion depends on random particle movements, substances diffuse across membranes without requiring the cell to use energy

26. What diffuses across the membrane??? Small, uncharged (non-polar) molecules Examples: Carbon dioxide Oxygen

27. Cells are always trying to Maintain Equilibrium by…3 ways… PASSIVE Transport 2 types Requires NO energy Goes with Conc. Gradient types: SIMPLE DIFFUSION- No protein required Small, uncharged particles FACILITATED DIFFUSION- CHANNEL or CARRIER proteins Trans-membrane protein channel Protein with a specific shape; open-close mechanism Ex. Osmosis and ligand-gated channels ACTIVE Transport 1 type REQUIRES Energy Goes Against Conc. Gradient ACTIVE TRANSPORT Involves “transporter” membrane protein and ENERGY (ATP)

28. Osmosis Water (H2O) can pass easily through most biological membranes Def: Diffusion of Water through a semi-permeable membrane Small molecules of water can sometimes diffuse across easily (no protein needed) AQUAPORIN: What does “aqua” mean??? Protein in cell membrane that allows water through (speeds up diffusion of water across the membrane) Type of Facilitated diffusion (more on this later)

29. How Osmosis works… We have water molecules and some other molecules, let’s say sugar If we have more water on one side of the membrane (HIGH conc. of H2O), then the water will diffuse across… Membrane will let water thru but not sugar Water can move back and forth (not sugar)

30. Water moves from areas of HIGH conc. To areas of LOW conc. Water moves across till equilibrium is reached Isotonic: when conc. Of both solutions is equal Hypertonic: “above strength” When we begin with more sugar in water More concentrated sugar solution on side A Hypotonic: “below strength” When we end with less sugar Dilute sugar solution on side A

35. Osmotic Pressure Pressure exerted by osmosis on the HYPERTONIC side of a semi-permeable membrane Think about blowing up a balloon… Can cause serious problems for cell Cell is full of sugar, salts, proteins, molecules Inside of cell is Hypertonic Osmotic pressure should make fresh water go…? In or Out? In Cell will become inflated/swollen Too much cell will burst like a balloon

36. Why don’t all our cells burst? What are our cells (animal) contained in? Fresh water? No…..blood or other fluids, which are ISOTONIC The conc. Of sugars, salts, proteins and molecules in these fluids is the same as the conc. in the cell Bacteria and plant cells They DO come in contact with fresh water…what do they have PROTECTING them? CELL WALL Prevent cells from expanding even when there’s a lot of osmotic pressure But, cell walls are prone to injuries when there is too much osmotic pressure

37. Which is a RBC in isotonic solution? RBC in hypertonic solution? RBC in hypotonic solution?

40. How do big molecules diffuse across a membrane so quickly if the membrane is selectively permeable?

41. Problems for diffusion… Things too large (like Glucose!) Charged molecules and Polar molecules Positive/negative Opposites attract but likes do not… These all present serious problems for things getting across membranes…

42. Facilitated Diffusion What does facilitate mean? Proteins are the extra help “escorts” across the membrane We call these membrane proteins… Protein channels Carrier proteins LIGAND receptor proteins

44. Facilitated Diffusion (continued) FAST SPECIFIC Still diffusion so we only see it from high concentration to low Does NOT require energy

46. What about when we want to go against the concentration? (From low concentration to high?)

47. What do we need??? ENERGY!!!!

48. ACTIVE transport Process that moves molecules against the concentration gradient Requires ENERGY A protein pumps small molecules and ions across a cell membrane against the conc. Gradient Direct and Indirect Active transport (see animation) Forms of molecular transport proteins are used to pump small molecules and ions across membrane even against the conc. grad. Direct: every 3 Na+ ions pumped across for every 2 K+ ions Indirect: Build up of ions on one side opens up another channel to shuttle in other molecules (Na+/Glucose channel) Electrochemical gradient membrane potential (cell membrane is negative) Na+ opens build up on the outside of the cell…draws water out of the cell so it doesn’t swell or burst! To pump large molecules and clumps… 2 other processes: Exocytosis: exo-means….cyto- means…..-sis means…. Endocytosis: endo-means….cyto- means…..-sis means…. They can change shape of membrane

49. Build up of Na+ ions on one side of membrane from Na+/K+ pump Now Na+ will flow thru another channel that allows one glucose in too! Pretty convenient!

50. Endocytosis When cells need to take in large material Process of taking material into the cell by process of infolding, or pockets, of the cell membrane Pocket breaks loose from cell membrane and forms a vacuole or vesicle inside the cell Two Types… Phagocytosis Pinocytosis

52. Two types of endocytosis: 1. Phagocytosis “cell eating” Extensions of cytoplasm surround the particle and package it within a food vesicle Cell then engulfs the package Amoebas 2. Pinocytosis “cell drinking” When cell needs to take up liquid Tiny pockets in cell membrane form Fill with liquid or many smaller molecules Then pinch off to form vesicles inside of cell

55. Exocytosis Exo- means… Cyto means… Sis means… When cell releases large amounts of material Excretes stuff Membrane of vacuole surrounding particle inside cell fuses with the cell membrane The contents in vacuole are then forced out of the cell We see this in removal of water by contractile vacuoles

58. Cells are always trying to Maintain Equilibrium by…3 ways… PASSIVE Transport 2 types Requires NO energy Goes with Conc. Gradient types: SIMPLE DIFFUSION- No protein required Small, uncharged particles FACILITATED DIFFUSION- CHANNEL or CARRIER proteins Trans-membrane protein channel Protein with a specific shape; open-close mechanism Ex. Osmosis and ligand-gated channels ACTIVE Transport 1 type REQUIRES Energy Goes Against Conc. Gradient ACTIVE TRANSPORT Involves “transporter” membrane protein and ENERGY (ATP)

59. MEMBRANE MAMBO!!!

60. Membrane Mambo 10 minutes to plan 2 teams/Cells Nucleus for each team Each member must be assigned a molecular role (must be labeled) Membrane protein(s) Molecule A (ex. Glucose) Molecule B (ex. Na+) Molecule C (ex. Water) Remember molecules are ALWAYS moving!!! MUST act out the following types of membrane transport: Simple Diffusion Osmosis Facilitated diffusion (channel, carrier and ligand) Active transport (direct and indirect) Be prepared to determine the concentration on either side of the membrane Your “Nucleus” must turn in a paper with every ones name and roles Summary of how each scenario will be acted out