1) The study developed a protocol for propagating the vulnerable medicinal orchid Spiranthes sinensis using rhizome section culture. 2) Rhizome sections were cultured on different concentrations of growth hormones including IBA, IAA, NAA, GA3, and cytokinin. 3) IBA at 100 ppm and GA3 at 500 ppm favored good germination and bud formation from the rhizome sections, with 100% and 90% survival rates respectively. The plantlets grew to 0.6 cm and 1.0 cm in length respectively.