Until now only the most elite laboratories have been able to build complex high-resolution tissue for drug screening and transplantation. Our technology has made building large 3D tissues as simple as pipetting your favorite cells.

1. BUILDING 3D TISSUES FOR
TRANSPLANTATION AND DRUG SCREENING
Consistent, transplantable large-format 3D tissue culture for
screening of rare cells in vitro and in vivo.
1

2. WHY SCREEN
THERAPEUTICS IN
3D CELL CULTURE?
2
3D cellular environments give more
accurate drug screening results
3D cellular environments induce
differential cell gene expression
Multi-cell 3D cultures better replicate
the in vivo environment
Improved human to animal translation

3. COMMON ROADBLOCKS FOR 3D TISSUE
CULTURE ADOPTION
3
Inconsistent Results
Inconsistent Spheroid Formation (not
all cells form spheroids)
Often spontaneously dissociate
Spheroids are only a few 1000 cells
Limited by Growth
Conditions
Long cell culture times to establish
Hypoxic core limits or alters cell
growth
High Overhead
Specialized training necessary (Masters
or PhD)
Complex protocols & specialized cell
culture methods
Limited Use Cases
Difficult to effectively transplant
spheroids as a tissue
Spheroids do not induce spontaneous
vascularization
Difficult to slice or image for structural
studies

4. VASCULAR SCAFFOLDS MAKE 3D CULTURE SIMPLE
4
Guaranteed 3D growth with any
cell type
Matrix of your choice may be used
Specialized training is not required
Standard 96 well plate format
Guaranteed 3D cell growth
Little to no hypoxia
Matrix of your choice may be used
Standard culture conditions
Specialized training not required
6 month shelf-life
100% engraftment of tumors with only
150,000 cells
FACS, IHC, IF and Imaging Compatible
Multiple cell types may be layered
Standard 96 well plate format
Ready to use in 24 hours

5. UNMATCHED RESOLUTION IN BIOCOMPATIBLE MATERIALS
5
• Prellis Biologics, Inc. uses proprietary laser-based printing methods produce high-fidelity, high-resolution
biocompatible 3D structures that match the features of native tissues.
• Each vascular feature is hollow, reducing hypoxia, and allowing for cell growth as a large format 3D
tissue system.
• VascularTissue BlanksTM provide consistent 3D architecture for tissue engineering applications.

6. SMALL 3D TISSUES
WITH HUGE R&D
POTENTIAL
6

7. TISSUE CHIP
• >1,000 vascular and microvascular features
• Suitable for transplantation
• Size: 4 x 4 x 1 mm
• Volume: 16 mm3
7

8. VASCULAR BUNDLE
• Suitable for layered and mixed cell seeding
• Over 50 vascular branches to support cell
niche formation
8

9. FREEFORM TISSUE STRUCTURES
9
BRIGHTFEILD + HUVECs SUBSTRUCTURE HUVECs stained for Laminin

10. CURRENT CUSTOMER FEEDBACK
10
"The setup is quick and consistent, and the handling is straightforward.There is a little pain, as in most cases, but a lot less than before. 100%
better." -Maria Soloveychik , Ph.D. CEO of SyntheX
"The structures are also more consistent, so less variability from that perspective too. Less variability means quicker, with less cost and fewer
tests – to results." -Marcus Muench, Ph.D. UCSF Professor
"Our cultures took significant time – 10 – 12 days or more, while Prellis is a 24 hour seed cycle to get started with your
experiment” – Folarin Erogbogbo, Ph.D. Professor SJSU
"You need a constant environment to get a reliable, accurate result across and between experiments.We have to always work in the same
reliable substructure.This is a big issue in 3D stem cell field.To do a small screen – variability is a big problem. [Traditional organoids] lack
consistency and don't have vasculature." -Jennifer Erwin, Ph.D.Assistant Professor John Hopkins School of Medicine
"Their technology provides a radical new dimension in our ability to control cell behaviour by tightly manipulating and controlling
micro-scale architecture of 3D culture." - Michael Jones, Ph.D. CEO Cell Guidance Systems

11. WHAT ARE VASCULAR SCAFFOLDS?
11
Seed cells in seconds
Hundreds of laser-printed vascular features Large format 3D tumor growth for up to 4 weeks
Oxygen permeable, biocompatible hydrogel that is laser-printed by Prellis Bio technology in designs that
mimic vascular-like channels and allow for large format tissue growth under standard conditions.
Human IPSCs

12. ORGANOID GROWTH AT D14
HYPOXIA (PDK1 expression)
12

13. ORGANOID GROWTH
Even with minimal HCT116 cell seeding organoids continue to grow for 3 weeks.
Cells remain viable for up to 4 weeks under standard tissue culture conditions.
13

14. EARLY ANIMAL STUDY DATA
14

15. HIGH BIOCOMPATIBILITY:
SCAFFOLDS TRANSPLANTED WITHOUT CELLS
15
2 WEEKS POST-TRANSPLANTATION 8 WEEKS POST-TRANSPLANTATION
RED: CD31 (PNAD)
BLUE: DAPI
GREEN: PRINTED STRUCTURE
RED: CD31 (PNAD)
BLUE: DAPI
GREEN: PRINTED STRUCTURE
Fine capillary growth along and inside
printed structure.
Larger blood vessel growth hundreds of
microns inside the transplanted structure.
20 µm

16. 16
TRANSPLANTATION OF 150,000 CELL-SEEDED
VASCULAR SCAFFOLDS
8 week mouse study – fat pad transplant:
Groups:
150,000 tumor cells (A549) + Collagen + vascular blank
150,000 tumor cells (A549) + Matrigel + vascular blank
(positive control) 2 million cells per standard Matrigel injection
(negative control) Structure only
2 million cells + matrigel 200K cells + collagen 200K cells + matrigel scaffold only
8 week Angiosense imaging

17. 17
TRANSPLANTATION OF 150,000 CELLS WITH AND
WITHOUT SCAFFOLD
Ongoing 6 week mouse study
scaffold only150,000 tumor cells + Matrigel 150,000 tumor cells + Matrigel + scaffold
Week 2 images

18. 18
TRANSPLANTATION OF 150,000 CELL SEEDED TUMORS
2 million cells + matrigel 200K cells + collagen 200K cells + matrigel scaffold only
Representative H&E (8 weeks)

19. 19
TRANSPLANTATION OF 150,000 CELL SEEDED TUMORS
2 million cells + matrigel 200K cells + collagen 200K cells + matrigel scaffold only
Representative IF (8 weeks)
RED: CD31 (PNAD)
BLUE: DAPI
GREEN: PRINTED STRUCTURE

20. IN VITRO 3D TUMOR STUDIES
20

21. IN VITRO STUDIES REPLICATE KEY 3D TUMOR INSIGHTS
21
Replicated key study showing that 2D screening can give false
positive results, in just 48 hours demonstrating statistical
significance with just n = 3.

22. IN VITRO STUDIES REPLICATE KEY 3D TUMOR INSIGHTS
22
Replicated key study showing that 2D screening can give
false negative results, in just 48 hours demonstrating
statistical significance with just n = 3.

23. DOSE RESPONSE CURVE IN
TUMOR ORGANOIDS
“These are far easier to set up and process than tumor spheroids” –
customer feedback from SyntheX
23
Compound A Compound B
Dose control control(+++) (++) (+) (+++) (++) (+)
Prellis Biologics collaborators independently
created and tested A549 tumor organoids.
Tumors were grown for 8 days and treated
with two peptide-based compounds,
demonstrating a dose-dependent response in
3D tissues.

24. ORGANOID+ HUVEC
HUVECs self-organize in 3D on
vascular tissue blanks.
24
DAPI
STRUCTURE
LAMININ
DAPI
LAMININ
LAMININ

25. VERSATILE 3D TISSUE CULTURE
25
HOWTO BUILDYOUR OWNTISSUE

26. WWW.PRELLISBIO.COM
CONTACT: INFO@PRELLISBIO.COM 26