This document discusses methods for simulating bacterial growth on brownfield sites to aid in revegetation efforts. It describes taking soil samples from various points on an industrial site and growing bacteria from those samples in a gelatin simulation over 30 days at 25 degrees Celsius. This small-scale simulation allows for an accelerated view of how bacteria and potential plant growth might occur on the brownfield, identifying remediation needs to support amendment and safe reuse of the land. The simulation provides time intervals for bacterial multiplication at different scales to optimize brownfield revegetation strategies.

1. Brownfield revegetation
prediction

2. Brownfield revegetation Why is it
important?
By amending brownfield sites we
 Make ground water safer
 Increase homeostasis
 create oxygen production
 form animal habitats

3. Soil formation

4. Soil formation 2
• Since soil bacteria is susceptible to climate,
time, and necessary for making nutrients
accessible for plant growth we concentrate on
them.

5. Industrial site in operation

6. Industrial site in operation 2
• By knowing the points of operation at the site
when in use one will know the points of
highest concentraion of soil contaminantschemical analysis to confirm. Seeing the plant
in operation you can identify the soil parent
material.

7. Present state of site
– Brownfield

8. Present state of site
• Taking soil from five points first the four points
shown in the picture the circumference or the
upper level soil (parent soil) then the fifth
point the lower flat level.

9. Brownfiled bacterial growth
simulation
• Simulation

10. Brownfiled bacterial growth
simulation method
• 112 grams of gelatin boile in 300 mL of water
until gelatinized 1 gram of soil form five points
of the present day site. Left in a 25 degree
celsius environment for 30 days.

11. Measure twice plant once
• Using a smaller simulated culture of the
brownfield allows for an accelerated
propagation giving the growth pattern for
bacteria,therefore, plant growth. The
simulation will show the effects of the
contaminations allowing for precise actions
needded for amendment and remediation of
the brownfield site.

12. Time interval per area

13. Time interval per area
• The graph shows the duration for bacteria that
multiply from 1E5 to 1.9E18 ( amount of
bacteria per acre) and 1E5 to 1.6E7 (amount
of bacteria on surface area of 2 cm2). Giving
an accelerated propogation.

14. Conclusion
• By using a small-scale brownfield simulation
one can increase the efficacy of amendment
reducing time, cost, and resources.