The document outlines the process used to sequence the human genome. It describes how DNA was broken into fragments, placed in bacterial chromosomes to be replicated, cut into fingerprints, and then reassembled by mapping overlapping sequences of letters to complete the genome sequence. The project took 7 years to complete at a cost of $3 billion and led to discoveries that could help map genes associated with diseases and develop new medical treatments and cures.

2.  Complete the sequence of DNA of the human
genome

3.  There are 35,000 human genes in our genome

4.  The method at use in this project is called a
hierarchical shotgun
 There are 7 main steps to the method

5.  Break down into a code of 150,000 letters

6.  Each fragment was placed into a bacterial
artificial chromosome
 The E. coli inside the chromosome would
replicate each DNA sample

7.  Special enzymes were used to cut each clone
into ‘fingerprint’ of fragments

8.  Combined the clones into a longer strand
 Mapped them onto the human chromosome

9.  Broke down the cloned fragments into 1000-
2000 based pairs long
 Generated a sequence of the individual bases

10.  Shotgun method was used to sequence the
fragments of human DNA

11.  Overlaps of the letters of the genetic code were
used to assemble the sequence of DNA

12.  The first major experiment to map the human
genome is performed on a sheep named Dolly
 Deeper concepts were found in this experiment

13.  Map out the single gene that causes genetic
diseases or disorders

14.  Discoveries in the medical fields
 Cures of diseases

15.  Costs 3 billion dollars
 High risk in the project

16.  People will not got a chance to live there lives
fairly
 DNA is going to determine the opportunities

17.  Someday will be able to tell the life someone is
going to live by there human genome