Mitocondrial genomic sequence variation as a useful maker for assessing genetic heterogeneity in Cyclospora cayatenensis ISABELA JARAMILLO RIVERA

1. PRESENTED BY: ISABELA JARAMILLO R.
III SEMESTER
2019 - 1

2. INTRODUCTION
MITOCHONDRIAL GENOME
Mitochondrias are organelles
ubiquitous to the cytoplasm of all
eukaryotic cells of animals, higher
plants, and some microorganisms.
Mitochondria generates energy
for cellular processes by
producing ATP through oxidative
phosphorylation.
Although most DNA is packaged
in chromosomes within the
nucleus, mitochondria also have a
small amount of its own DNA.
This genetic material is known as
mtDNA.
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Cyclospora Cayetanensis
Is an intestinal coccidian
protozoan parasite. It has a
worldwide distribution, and
transmission is through the
fecal-oral route or by
ingestion of contaminated
water or food.
This parasite infects the
gastrointestinal tract of
returning travelers , causing
acute diarrheal disease.

3. 3
INTRODUCTION
As an eukaryotic parasite, C. cayetanensis has a
mitochondrial genome. As mitochondrial genomes are
evolving more quickly than nuclear genomes , molecular
typing assays developed based on mitochondrial
sequences generally have high resolution.
The mtDNA of C. cayetanensis has about 500 copies per
cell; molecular diagnostic tools targeting mitochondrial
sequences may have the additional advantage of higher
detection sensitivity than those targeting nuclear genes,
making the mitochondrial genome a potential marker for
the development of a genotyping tool .

4. 4
Overall
Objective
Determine
mitocondrial
genomic sequence
variation as a useful
maker for assessing
genetic
heterogeneity in
Cyclospora
cayetanensis

5. 5
MATERIALES Y MÉTODOS
FUNDAMENTO
• Técnica de amplificación enzimática In vitro donde se
obtienen millones de copias de un fragmento específico
de ADN.
• Genotipos en el estudio: CM 1  CM 9
APLICACIÓN
• qPCR amplifica y cuantifica simultáneamente el producto del
DNA replicado.
• Durante la investigación, la qPCR, fue utilizada para hacer la
cuantificación del genoma en las diferentes muestras y para
analizar la cantidad de expresión existente.
qPCR

6. 6
MATERIALES Y MÉTODOS

7. RESULTADOS
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Se evidencia una
amplificación en
todos los genotipos
intervenidos, con
una variación
mínima
imperceptible

8. 8
RESULTADOS

9. 9
RESULTADOS

10. DISCUSSION
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“In a previous study, eight single-nucleotide variants
(SNVs) and one 7-bp multiple-nucleotide variant
(MNV) in the mitochondrial genomes were detected
between two C. cayetanensis isolates from China and
the USA, making the mitochondrial genome a
potential marker for the development of a
genotyping tool”.
AUTHOR CONTRIBUTION OR
“In theory, the qPCR developed in this study could be
used for rapid assessment of genetic heterogeneity in C.
cayetanensis among specimens in events of augmented
number of cyclosporiasis cases. Previously, a MLST assay
targeting five loci with short tandem repeats (STRs) was
developed for C. cayetanensis”.
Guo Y, et al.
“Although large cyclosporiasis outbreaks have
occurred almost annually in North America and some
European countries during the past 20 years, the
prevention and control of cyclosporiasis outbreaks
are still a major public health challenge”.
Hedberg CW, et al.
Tang K, et al.

11. 11
CONCLUSSIONS
The availability of
Cyclospora
cayetanensis genome
sequence provides
opportunities for
research and
development of
multiple other
diagnostic tools.
The evaluation of genetic
polymorphisms in the same
parasitic species, caused by
geographical variability, can
detect different behaviors
of the same pathogen, which
is useful to focus a more
specific treatment taking
into account the genomic
versatility.

12. MAPA CONCEPTUAL
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