The document discusses the rising concerns over brain health and mental illnesses, emphasizing the importance of nutrition in treating and preventing these conditions. It highlights the role of omega-3 fatty acids, specifically EPA and DHA, along with various vitamins and minerals essential for cognitive function, mood regulation, and overall brain health. Additionally, it introduces Mindcare® supplements, designed to provide targeted brain nutrition based on consumer needs, containing scientifically proven ingredients for optimal mental well-being.

1. Brain-boosting bio-actives:
what really works?

2. Why brain health is HOT right now
• 1 in 4 suffer with a mental health condition
• 12 million adults see their GP for this – most cases stress-
related anxiety and depression
• 13.3 million working days lost each year due to stress-related
illness
• Cognitive decline and dementia are on the rise
• 1 in 5 older adults suffer with depression and/or dementia
• 7% of over 65s have dementia
• 20% of over 80s have dementia
Issues affecting the brain and mind are widespread

3. The Problem

4. With nearly all of UK adults affected by one of the four following need-states
determining how we can utilise nutrition to treat and prevent mental illness is of key
public health importance

5. Introducing MindCare®
Having specialised in omega-3 lipid therapeutics for over a decade, we have
translated our extensive expertise from our cutting edge science-based omega-3
EPA supplements and our understanding of brain development, cognition and
mental health into a premium mass market wellbeing offering for adults seeking to
make the most of life. MindCare® supplements deliver the foundational
ingredients for the brain and all-round health to transform how people feel.

6. Nutrition for brain and mental health –
the evidence

7. Brain studies are extremely difficult to conduct
Why??
What's the optimal
length of time for
this intervention
Which nutrients should
be used? Single, isolated
nutrients or blends….
What dose do we
give?
What's the right
population for this study
and intervention?
What else might be affecting
the participants’ brains?

8. Omega-3s

9. Lipids and the brain 18 = Stearic
18:2 = Linoleic
20:3 = DGLA
20:4 = AA
22.5 = DPA
18.3 = α-LA
20.5 = EPA
22.5 = DPA
22.6 = DHA

10. EFSA panel outcomes of DHA:
• The Panel notes that there is a well established role of DHA in
brain function
• The Panel concludes that a cause and effect relationship has
been established between the consumption of DHA and the
maintenance of normal brain function
• The Panel considers that in order to bear the claim, foods
should contain 250 mg of DHA in one or more servings
http://www.efsa.europa.eu/sites/default/files/scientific_output/
files/main_documents/3840.pdf

11. • Dominant polyunsaturated fatty acid in the brain
• DHA vital for structure of the brain
• Own unique structure ensures membranes are fluid and flexible
• Vital neuroprotection
– neurotransmission, membrane channel and receptor function,
synaptic plasticity
– memory and learning
DHA and the brain

12. • Structurally, EPA not a key player but numerous other roles
make it vital for brain structure and function
– protects DHA via reducing phospholipase A2 activity
– major anti-inflammatory eicosanoid precursor – directly competes with
and displaces AA to reduce inflammation
– interacts with genes directly to stimulate neurone growth and
differentiation
– reduces oxidative stress
– enhances focus, attention and processing speed
– rapid β-oxidation in preference to DHA providing fuel for mitochondrial
energy production
EPA and the brain

13. • EPA directly reduces stress perception and
protects against HPA-axis dysregulation
• Reducing inflammation protects against
degradation and reduced transportation of
neurotransmitters at the synapse
Repeated exposure to stress = HPA-axis hyper
responsiveness, anxiety, depression,
inflammation, oxidative stress and increased cell
ageing
Bosma-den boer 2012, Laugero et al., 2011Furuyashiki 2012
Keikolt-Glaser., 2010, Ros., 2009
EPA and the brain

14. • EPA turnover considerably higher than
DHA (brain DHA turnover is very low 4-5mg/day)
• Requires constant replenishment at
higher levels than DHA
– protects against the high levels of
inflammation, oxidative stress and HPA-axis
dysregulation, common to modern diet and
lifestyles
• EPA in excess of DHA – necessary to elicit
mood and anti-inflammatory benefits
EPA and the brain
Roke and Mutch 2014:
Supplementation with 1200mg EPA +
600mg DHA in young healthy males
ages 18-25 for 12 weeks followed by 6
weeks washout

15. BUT - does your fish oil deliver?

16. Importance of oil concentration
 Higher concentrations increase cellular omega-3 levels more than the same
dose provided at a lower concentration
Brunton and Collins 2007

17. Importance of dose plus concentration
 Higher dose high concentrations from rTG fish oil increase cellular omega-3
levels up to 5x more than krill oil and 3x more than standard fish oil
Laidlaw et al.,
2014
Comparison of
manufacturer-
recommended
dose of rTG, EE
concentrated
fish oils with Krill
oil (PL) and
salmon oil (TG)

18. The power of rTG omega-3
Dyerberg et al., 2010 graph shows the % increase in serum EPA+DHA content following 2 weeks of EPA
and DHA supplementation Av. 3.3g per day.
 rTG oil delivered biggest increase in serum lipid content in the lowest volume of oil and
lowest total dose of EPA+DHA (all others delivered 200mg EPA + DHA or more)

19. B vitamins

20. • Vital for energy metabolism, synthesis of
neurotransmitters, antioxidant protection
• Ensures optimal delivery of fuel to the brain
– enhances cognition, relaxation, sleep, mental focus and
reduces stress and oxidative stress
B vitamin complex

21. • Homocysteine recycling – by-product of the methylation cycle – necessary
for the production of:
– SAMe – metabolism of neurotransmitters serotonin, melatonin and dopamine
– cysteine and glutathione – vital for antioxidant protection and detoxification processes
– DNA – cell cycle, genetic replication, growth and development
– carnitine, choline and CoQ10 – energy metabolism and mitochondrial function
– myelin proteins – nerve transmission and CNS communication
• Homocysteine accumulation affects every function right down to the
cellular level
B vitamin complex

22. Other vital vitamins

23. Vitamin C
• further supports detoxification, provides antioxidant
protection against free radicals
• reduces tiredness and fatigue
• necessary for the proper functioning of the CNS and
psychological functioning
Vitamin E
• sunflower seed source
• antioxidant protection
Vital vitamins

24. Vital vitamins
Vitamin D
• expression of nootropics (‘smart’ drugs)
• neurone growth and development
• calcium homeostasis – neurone firing
• detoxification - helping to rid the brain of
protein build-ups

25. Mind-modulating minerals

26. Zinc
• essential to the production of neurotransmitters
• enhances neurotransmission via interaction with receptors, transporters
and ion channels in the neurone and synapse
Selenium
• up-regulates glutathione production
• main component of antioxidant enzymes
• supports proper adrenal function – commonly disrupted by high stress and
poor diet – leads to poor sleep, memory problems and fatigue

27. Magnesium
• Regulates the CNS via
– neurotransmitter synthesis
– neurone activity
– synaptic plasticity

28. Immune function and the brain

29. • Stress, busy lifestyles, poor diet, ageing, poor sleep
and low mood all negatively impact the immune
system
• When we get sick, brain function and mood are
dramatically reduced
• Vitamin C, E, D, zinc, selenium, omega-3 and B
vitamins all work together to enhance, support and
protect immune function
• Reduces likelihood of illness
• Prevents stress-induced immune weakening

30. Immune function and the brain

31. Stress and anxiety

32. Chronic stress
HPA axis over-
stimulation
Excess cortisol Inflammation
Oxidative stress,
lipid peroxidation
Low serotonin, GABA and dopamine, anxiety, depression,
tiredness, fatigue, immune suppression

33. Chronic stress
HPA axis over-
stimulation
Excess cortisol Inflammation
Oxidative stress,
lipid peroxidation
Low serotonin, GABA and dopamine, anxiety, depression,
tiredness, fatigue, immune suppression
Omega -3

34. Chronic stress
HPA axis over-
stimulation
Excess cortisol Inflammation
Oxidative stress,
lipid peroxidation
Low serotonin, GABA and dopamine, anxiety, depression,
tiredness, fatigue, immune suppression
Vitamins
C and B

35. Selenium protects thyroid
function, reducing impact of
HPA-axis overstimulation
Vitamin B5 helps regulates
adrenal function – reducing
hypersensitivity to stress

36. Magnesium
• Neurotransmitter production
• Vitamin B6 absorption
• Required by 325 enzymes (many of which
act in the brain)
• Neurone health, synaptic plasticity,
learning and memory [51,52]
• Low magnesium levels linked to anxiety, depression,
irritability, insomnia, confusion….[50]

37. L-Theanine
• Amino acid commonly lacking in modern diet
• Present in tea!
Serotonin
Happiness
Sleep
Dopamine
Focus
Motivation
Learning
Anti-anxiety
GABA
Relaxation

38. Ashwagandha
Valerian
Rhodiola
Reduction of stress
and insomnia
Anti-anxiety
benefits
Prevents high
cortisol production
Protects against stress induced
immunosuppression
Minor benefits to
anxiety sleep and
fatigue
Increased subjective
wellbeing in stressed
fatigued individuals

39. Focus, attention and cognitive
performance

40. Omega-3 increases blood flow to the brain supplying oxygen and fuel delivery, are
essential for neurotransmitter production and function, memory, learning, cognition, and
brain and neurone cell structure
Benefits restricted to those with sub-optimal omega-3
intake – surprised?!

42. DHA is for memory and
learning if intake is low
EPA in excess of DHA for
cognitive performance, in
particular attention
Total omega-
3 needed to
be >400mg
‘DHA only’
often resulted
in detrimental
effects to
cognition
Many benefits of DHA
associated with increased
blood flow
>1month intervention needed
for benefits to be seen

43. Zinc is necessary for neurotransmitter production and helps to
ensure sustained, optimal neurone firing

44. Acetyl-L-Carnitine
• Transports fat directly to the mitochondria of brain cells
• Increases noradrenaline, dopamine and serotonin and their receptors
– increases memory and cognitive function

45. L-Theanine PLUS caffeine
• Potent fine-tuning of focus, concentration and memory
• Improved cognitive performance for demanding tasks

46. Taurine is an amino sulfonic acid that acts as a lipid/membrane stabiliser in the
body and can aid various antioxidant defence systems.

47. Amino Acids. 2000;19(3-4):635-42.
A taurine and caffeine-containing drink stimulates cognitive performance and well-being.
Seidl R1, Peyrl A, Nicham R, Hauser E.
The findings clearly indicate that the mixture of three key ingredients of Red Bull Energy
Drink used in the study (caffeine, taurine, glucuronolactone) have positive effects upon
human mental performance and mood.
Psychopharmacology (Berl). 2001 Nov;158(3):322-8.
An evaluation of a caffeinated taurine drink on mood, memory and information processing
in healthy volunteers without caffeine abstinence.
Warburton DM1, Bersellini E, Sweeney E.
RESULTS:
In both studies, the caffeinated, taurine-containing beverage produced improved attention
and verbal reasoning, in comparison with a sugar-free and the sugar-containing drinks. The
improvement with the verum drink was manifested in terms of both the mean number
correct and the reaction times. Another important finding was the reduction in the variability
of attentional performance between participants.

48. • L-Theanine + taurine calm and focus the mind via GABA and
dopamine activation
• Caffeine stimulates the brain, increasing energy, alertness and
information processing speed
• L-Theanine + caffeine enhance focus and reduce distractibility

49. Mood balance

50. Omega-3
• EPA and DHA are essential for mood-regulating
neurotransmitter production and function
• EPA reduces inflammation, which directly attacks and
degrades serotonin, leading to low mood and depression

51. Depression Sublette 2011 Meta-analysis

52. Grosso et
al., 2014
http://ww
w.ncbi.nlm.
nih.gov/pu
bmed/2480
5797

53. Vitamin D
• acts as a mood stabiliser
• low levels increase risk of anxiety and depression
• Studies show mixed results (in some case worsening) in
managing depression

54. ‘all studies without flaws demonstrated a statistically significant
improvement in depression with Vitamin D supplements…… the
effect size was comparable to that of anti-depressant medication.’
NB: Only effective in those who are deficient AND dose given must
result in a changed serum Vit D level
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4011048/

55. B Vitamins
• Vitamin B3 helps to reduce inflammation and increase
dopamine synthesis [14]
• Vitamin B7 helps to stabilise blood sugars and reduce mood
swings [30,31]
• B12 and folate – vital for homocysteine recycling (and
therefore SAMe production) – levels linked to positive affect in
healthy population (Edney et al., sept 2015 http://www.ncbi.nlm.nih.gov/pubmed/26346363)
• B6 (P5P) positively correlates with tryptophan levels and
reduced kynurenine pathway metabolites
(Deac et al., April 2015 http://www.ncbi.nlm.nih.gov/pubmed/25833774)

56. Recent study using NHANES data found significant
relationship between very low magnesium status and
depression
Tarleton and Littenberg March 2015 http://www.ncbi.nlm.nih.gov/pubmed/25748766
Magnesium
Supplementation with
glycinate/taurinate form (1-
300mg <4 times daily) shown
to quickly and effectively
relieve depression symptoms
[50]

57. 5-HTP
Is the direct precursor to serotonin
and unlike tryptophan cannot be
used for niacin or protein synthesis
It easily crosses the blood brain
barrier
Currently, St John’s Wort is the much
better researched source and
contains approx. 25% active 5-HTP

58. Cognitive decline

60. BUT almost all studies of omega-3 use to boost
cognitive function have shown little or no
benefits – why?
• Study population
• Dose given
• EPA or DHA
• Cognitive tests chosen
• Duration of intervention time
• Is it already too late?

61. B vitamins
• B1,2,3 and 5
– support mitochondria of the brain and CNS
– aid detoxification pathways
– reduce inflammation
• B6, B12 and folate in their most active and bioavailable forms
– homocysteine recycling
– elevated levels = significant risk factor for age-related cognitive decline

64. Alpha-lipoic acid
– reverses age-related oxidative damage
– metal chelator – prevents build-up in blood
and brain
– stabilises mitochondrial membranes
– improves age-related cognitive decline
[70, 71, 72]

65. MMSE scores showed a significant improvement in 43% patients of group A (26 subjects)
and 23% of group B (15 subjects), compared to baseline (𝑃 = .001). Also ADAS-Cog,
CIBIC, and ADFACS scores showed a significant improvement in group A versus group B.
IR was higher in group A. Our study suggests that ALA therapy could be effective in
slowing cognitive decline in patients with AD and IR.

66. Resveratrol
– supports mitochondrial function
– slows ageing process via interaction with
sirtuin enzymes

67. Antioxidant combination formulas supply potent antioxidant
defence and recycling of body’s antioxidant pool to help reduce
free radical damage to neurones and protect brain structure and
function

68. Ginseng has been shown to provide mild
cognitive enhancement as well as improve
subjective wellbeing
Bacopa Monnieri
Was found to increase cognition and
improve symptoms of cognitive
decline after 4-6 weeks of use
Gingko Biloba studies have found
it provides a notable benefit to
cognition and symptoms of cognitive
decline

69. What else?

71. MindCare® - product range
Igennus MindCare® is the first comprehensive range of targeted brain nutrition
supplements based on four identified consumer need-states.
Transform how you feel™

72. 72
Asimple,expertlyformulated,1-a-daydualcapsulesystem
Ultra concentrated
MindCare® omega-3 EPA &
DHA capsules with vitamins
D & E
Precisely formulated to target and support brain
function (250mg DHA plus 410 mg EPA per
capsule) using the body-ready rTG form of
omega-3 that is nature-identical and easily
absorbed by the body
MindCare® micronutrient
capsules contain:
full B complex plus zinc,
selenium, vitamin C and
targeted ACTIVES
Target distinct areas of brain health with a
comprehensive blend of synergistic vitamins,
minerals and specialist actives at proven,
effective levels and in super-bioavailable forms
 MindCare® is based on cutting edge nutrition science, and combines premium
triglyceride omega-3 fish oil containing 80% active doses of EPA and DHA with
scientifically proven nutrients for various aspects of brain health

73. MindCare® BALANCE is designed specifically to target the
physiological changes associated with stress and feeling
overwhelmed, to help you stay relaxed and keep on top of life
With: Magnesium
glycinate and L-Theanine

74. MindCare® FOCUS is designed to optimise focus and
attention, allowing you to stay alert and fulfil your potential
when you need it most
With: Acetyl-L-Carnitine,
L-Theanine, taurine
and caffeine

75. MindCare® LIFT supplies the nutrients needed to protect
neurotransmitter production and function, boosting serotonin
naturally, to help you stay happy and enjoy life
With: Magnesium
glycinate and 5-HTP

76. MindCare® PROTECT is uniquely formulated to support brain
function as we age and protect against oxidative stress-induced
damage, to help you stay sharp and get the most out of life
With: Acetyl-L-Cysteine,
alpha lipoic acid and
resveratrol

77. • Highly researched and expertly formulated for maximum benefits

• Contains highly bioavailable omega-3 EPA and DHA
– nourishes, protects and repairs the brain and neurone structures
– enhances cellular communication and blood flow
– prevents oxidative & inflammatory damage
MindCare®
Ingredients summary

78. • Active, bioavailable and specialised
micronutrient blends
– mitochondrial function
– neurotransmitter production and
function
– antioxidant protection and detoxification
– reduce overstimulation of HPA-axis and
CNS in response to stress
– support natural biological functions
needed for optimal brain health
MindCare®
Mechanisms summary

79. MindCare®
Benefits summary
Calms the
mind, helping
you to regain
control and stay
relaxed and
keep on top of
life.
Fine tunes your
attention &
supercharges
your mental
processing so
you can stay
alert to fulfil
your full
potential.
For those
needing a little
pick-me-up.
Protects &
enhances your
feel-good
chemicals so you
can stay happy
and enjoy life.
Helps adults to
stay sharp by
protecting the
brain structure,
memory and
mood as you age,
so you can get
the most out of
life.

80. References
1. Blass JP, Gleason P, Brush D, DiPonte P, Thaler H: Thiamine and Alzheimer's disease. A pilot study. Arch Neurol 1988, 45(8):833-835.
2. Meador K, Loring D, Nichols M, Zamrini E, Rivner M, Posas H, Thompson E, Moore E: Preliminary findings of high-dose thiamine in
dementia of Alzheimer's type. J Geriatr Psychiatry Neurol 1993, 6(4):222-229.
3. Meador KJ, Nichols ME, Franke P, Durkin MW, Oberzan RL, Moore EE, Loring DW: Evidence for a central cholinergic effect of high-dose
thiamine. Ann Neurol 1993, 34(5):724-726.
4. Gibson GE, Hirsch JA, Cirio RT, Jordan BD, Fonzetti P, Elder J: Abnormal thiamine-dependent processes in Alzheimer's Disease. Lessons
from diabetes. Mol Cell Neurosci 2013, 55:17-25.
5. Depeint F, Bruce WR, Shangari N, Mehta R, O'Brien PJ: Mitochondrial function and toxicity: role of the B vitamin family on mitochondrial
energy metabolism. Chem Biol Interact 2006, 163(1-2):94-112.
6. Palabiyik B, Jafari Ghods F, Onay Ucar E: A potential protective role for thiamine in glucose-driven oxidative stress. Genet Mol Res 2014,
13(3):5582-5593.
7. Mair RG, Anderson CD, Langlais PJ, McEntee WJ: Thiamine deficiency depletes cortical norepinephrine and impairs learning processes in
the rat. Brain Res 1985, 360(1-2):273-284.
8. Mair RG, Otto TA, Knoth RL, Rabchenuk SA, Langlais PJ: Analysis of aversively conditioned learning and memory in rats recovered from
pyrithiamine-induced thiamine deficiency. Behav Neurosci 1991, 105(3):351-359.
9. Benton D, Griffiths R, Haller J: Thiamine supplementation mood and cognitive functioning. Psychopharmacology (Berl) 1997, 129(1):66-
71.
10. Ashoori M, Saedisomeolia A: Riboflavin (vitamin B2) and oxidative stress: a review. Br J Nutr 2014:1-7.
11. Weil-Malherbe H, Beall GD: Riboflavin 5'-phosphate: a potent activator or brain glutaminase. J Neurochem 1970, 17(7):1101-1103.
12. Green KN, Steffan JS, Martinez-Coria H, Sun X, Schreiber SS, Thompson LM, LaFerla FM: Nicotinamide restores cognition in Alzheimer's
disease transgenic mice via a mechanism involving sirtuin inhibition and selective reduction of Thr231-phosphotau. J Neurosci 2008,
28(45):11500-11510.
13. Gong B, Pan Y, Vempati P, Zhao W, Knable L, Ho L, Wang J, Sastre M, Ono K, Sauve AA et al: Nicotinamide riboside restores cognition
through an upregulation of proliferator-activated receptor-gamma coactivator 1alpha regulated beta-secretase 1 degradation and
mitochondrial gene expression in Alzheimer's mouse models. Neurobiol Aging 2013, 34(6):1581-1588.
14. Wakade C, Chong R: A novel treatment target for Parkinson's disease. J Neurol Sci 2014, 347(1-2):34-38.
15. Lin SH, Chong ZZ, Maiese K: Nicotinamide: A Nutritional Supplement that Provides Protection Against Neuronal and Vascular Injury. J
Med Food 2001, 4(1):27-38.
16. Kirkland JB: Poly ADP-ribose polymerase-1 and health. Exp Biol Med (Maywood) 2010, 235(5):561-568.
17. Underhill C, Toulmonde M, Bonnefoi H: A review of PARP inhibitors: from bench to bedside. Ann Oncol 2011, 22(2):268-279.
18. Azhgikhin IS, Gandel VG, Mekhtikhanov SD, Finkel VV, Serebriannikov NV: [Preparation of a concentrate of eicosapentaenoic and
docosahexaenoic acid esters as a possible substitute for arachidene and linetol]. Farmatsiia 1979, 28(5):18-21.
19. Kelly GS: Pantothenic acid. Monograph. Altern Med Rev 2011, 16(3):263-274.
20. Martinez DL, Tsuchiya Y, Gout I: Coenzyme A biosynthetic machinery in mammalian cells. Biochem Soc Trans 2014, 42(4):1112-1117.

81. 21. Jaroenporn S, Yamamoto T, Itabashi A, Nakamura K, Azumano I, Watanabe G, Taya K: Effects of pantothenic acid supplementation on
adrenal steroid secretion from male rats. Biol Pharm Bull 2008, 31(6):1205-1208.
22. Ramakrishna T: Vitamins and brain development. Physiol Res 1999, 48(3):175-187.
23. Douaud G, Refsum H, de Jager CA, Jacoby R, Nichols TE, Smith SM, Smith AD: Preventing Alzheimer's disease-related gray matter atrophy
by B-vitamin treatment. Proc Natl Acad Sci U S A 2013, 110(23):9523-9528.
24. de Jager CA, Oulhaj A, Jacoby R, Refsum H, Smith AD: Cognitive and clinical outcomes of homocysteine-lowering B-vitamin treatment in
mild cognitive impairment: a randomized controlled trial. Int J Geriatr Psychiatry 2012, 27(6):592-600.
25. Smith AD, Smith SM, de Jager CA, Whitbread P, Johnston C, Agacinski G, Oulhaj A, Bradley KM, Jacoby R, Refsum H: Homocysteine-
lowering by B vitamins slows the rate of accelerated brain atrophy in mild cognitive impairment: a randomized controlled trial. PLoS
One 2010, 5(9):e12244.
26. Depeint F, Bruce WR, Shangari N, Mehta R, O'Brien PJ: Mitochondrial function and toxicity: role of B vitamins on the one-carbon transfer
pathways. Chem Biol Interact 2006, 163(1-2):113-132.
27. Kim SY, Yoo JY, Ohe JY, Lee JW, Moon JH, Kwon YD, Heo JS: Differential expression of osteo-modulatory molecules in periodontal
ligament stem cells in response to modified titanium surfaces. Biomed Res Int 2014, 2014:452175.
28. Elias MF, Robbins MA, Budge MM, Elias PK, Brennan SL, Johnston C, Nagy Z, Bates CJ: Homocysteine, folate, and vitamins B6 and B12
blood levels in relation to cognitive performance: the Maine-Syracuse study. Psychosom Med 2006, 68(4):547-554.
29. Sweetman FR, Gibson KM, Sweetman L, Nyhan WL, Chin H, Swartz W, Jones OW: Activity of biotin-dependent and GABA metabolizing
enzymes in chorionic villus samples: potential for 1st trimester prenatal diagnosis. Prenat Diagn 1986, 6(3):187-194.
30. Lustman PJ, Clouse RE: Depression in diabetic patients: the relationship between mood and glycemic control. J Diabetes Complications
2005, 19(2):113-122.
31. Larrieta E, Vega-Monroy ML, Vital P, Aguilera A, German MS, Hafidi ME, Fernandez-Mejia C: Effects of biotin deficiency on pancreatic islet
morphology, insulin sensitivity and glucose homeostasis. J Nutr Biochem 2012, 23(4):392-399.
32. Moretti R, Torre P, Antonello RM, Cattaruzza T, Cazzato G, Bava A: Vitamin B12 and folate depletion in cognition: a review. Neurol India
2004, 52(3):310-318.
33. McCracken C: Challenges of long-term nutrition intervention studies on cognition: discordance between observational and intervention
studies of vitamin B12 and cognition. Nutr Rev 2010, 68 Suppl 1:S11-15.
34. Bowman GL: Ascorbic acid, cognitive function, and Alzheimer's disease: a current review and future direction. Biofactors 2012,
38(2):114-122.
35. Joshi YB, Pratico D: Vitamin E in aging, dementia, and Alzheimer's disease. Biofactors 2012, 38(2):90-97.
36. Balion C, Griffith LE, Strifler L, Henderson M, Patterson C, Heckman G, Llewellyn DJ, Raina P: Vitamin D, cognition, and dementia: a
systematic review and meta-analysis. Neurology 2012, 79(13):1397-1405.
37. Llewellyn DJ, Lang IA, Langa KM, Muniz-Terrera G, Phillips CL, Cherubini A, Ferrucci L, Melzer D: Vitamin D and risk of cognitive decline in
elderly persons. Arch Intern Med 2010, 170(13):1135-1141.
38. Hancock SM, Finkelstein DI, Adlard PA: Glia and zinc in ageing and Alzheimer's disease: a mechanism for cognitive decline? Front Aging
Neurosci 2014, 6:137.
39. Norgaard-Nielsen K, Gether U: Zn2+ modulation of neurotransmitter transporters. Handb Exp Pharmacol 2006(175):1-22.
40. Pifl C, Wolf A, Rebernik P, Reither H, Berger ML: Zinc regulates the dopamine transporter in a membrane potential and chloride
dependent manner. Neuropharmacology 2009, 56(2):531-540.

82. 41. Szewczyk B: Zinc homeostasis and neurodegenerative disorders. Front Aging Neurosci 2013, 5:33.
42. Brewer GJ, Kanzer SH, Zimmerman EA, Molho ES, Celmins DF, Heckman SM, Dick R: Subclinical zinc deficiency in Alzheimer's disease and
Parkinson's disease. Am J Alzheimers Dis Other Demen 2010, 25(7):572-575.
43. Vashum KP, McEvoy M, Milton AH, McElduff P, Hure A, Byles J, Attia J: Dietary zinc is associated with a lower incidence of depression:
findings from two Australian cohorts. J Affect Disord 2014, 166:249-257.
44. Mocchegiani E, Bertoni-Freddari C, Marcellini F, Malavolta M: Brain, aging and neurodegeneration: role of zinc ion availability. Prog
Neurobiol 2005, 75(6):367-390.
45. Santos JR, Gois AM, Mendonca DM, Freire MA: Nutritional status, oxidative stress and dementia: the role of selenium in Alzheimer's
disease. Front Aging Neurosci 2014, 6:206.
46. Brown KM, Arthur JR: Selenium, selenoproteins and human health: a review. Public Health Nutr 2001, 4(2B):593-599.
47. Senol N, Naziroglu M, Yuruker V: N-acetylcysteine and selenium modulate oxidative stress, antioxidant vitamin and cytokine values in
traumatic brain injury-induced rats. Neurochem Res 2014, 39(4):685-692.
48. Luchtman DW, Song C: Cognitive enhancement by omega-3 fatty acids from child-hood to old age: findings from animal and clinical
studies. Neuropharmacology 2013, 64:550-565.
49. Bryhn M, Hansteen H, Schanche T, Aakre SE: The bioavailability and pharmacodynamics of different concentrations of omega-3 acid
ethyl esters. Prostaglandins Leukot Essent Fatty Acids 2006, 75(1):19-24.
50. Eby GA, Eby KL: Rapid recovery from major depression using magnesium treatment. Med Hypotheses 2006, 67(2):362-370.
51. Xu ZP, Li L, Bao J, Wang ZH, Zeng J, Liu EJ, Li XG, Huang RX, Gao D, Li MZ et al: Magnesium protects cognitive functions and synaptic
plasticity in streptozotocin-induced sporadic Alzheimer's model. PLoS One 2014, 9(9):e108645.
52. Slutsky I, Abumaria N, Wu LJ, Huang C, Zhang L, Li B, Zhao X, Govindarajan A, Zhao MG, Zhuo M et al: Enhancement of learning and
memory by elevating brain magnesium. Neuron 2010, 65(2):165-177.
53. Ozturk S, Cillier AE: Magnesium supplementation in the treatment of dementia patients. Med Hypotheses 2006, 67(5):1223-1225.
54. Foxe JJ, Morie KP, Laud PJ, Rowson MJ, de Bruin EA, Kelly SP: Assessing the effects of caffeine and theanine on the maintenance of
vigilance during a sustained attention task. Neuropharmacology 2012, 62(7):2320-2327.
55. Bryan J: Psychological effects of dietary components of tea: caffeine and L-theanine. Nutr Rev 2008, 66(2):82-90.
56. Owen L, Sunram-Lea SI: Metabolic agents that enhance ATP can improve cognitive functioning: a review of the evidence for glucose,
oxygen, pyruvate, creatine, and L-carnitine. Nutrients 2011, 3(8):735-755.
57. Smeland OB, Meisingset TW, Borges K, Sonnewald U: Chronic acetyl-L-carnitine alters brain energy metabolism and increases
noradrenaline and serotonin content in healthy mice. Neurochem Int 2012, 61(1):100-107.
58. Xu S, Waddell J, Zhu W, Shi D, Marshall AD, McKenna MC, Gullapalli RP: In vivo longitudinal proton magnetic resonance spectroscopy on
neonatal hypoxic-ischemic rat brain injury: Neuroprotective effects of acetyl-L-carnitine. Magn Reson Med 2014.
59. Kamimori GH, McLellan TM, Tate CM, Voss DM, Niro P, Lieberman HR: Caffeine improves reaction time, vigilance and logical reasoning
during extended periods with restricted opportunities for sleep. Psychopharmacology (Berl) 2014.
60. Dixit A, Goyal A, Thawani R, Vaney N: Effect of caffeine on information processing: evidence from stroop task. Indian J Psychol Med 2012,
34(3):218-222.

83. 61. Tieges Z, Snel J, Kok A, Plat N, Ridderinkhof R: Effects of caffeine on anticipatory control processes: evidence from a cued task-switch
paradigm. Psychophysiology 2007, 44(4):561-578.
62. Tieges Z, Snel J, Kok A, Wijnen JG, Lorist MM, Richard Ridderinkhof K: Caffeine improves anticipatory processes in task switching. Biol
Psychol 2006, 73(2):101-113.
63. Seidl R, Peyrl A, Nicham R, Hauser E: A taurine and caffeine-containing drink stimulates cognitive performance and well-being. Amino
Acids 2000, 19(3-4):635-642.
64. Warburton DM, Bersellini E, Sweeney E: An evaluation of a caffeinated taurine drink on mood, memory and information processing in
healthy volunteers without caffeine abstinence. Psychopharmacology (Berl) 2001, 158(3):322-328.
65. Foos TM, Wu JY: The role of taurine in the central nervous system and the modulation of intracellular calcium homeostasis. Neurochem
Res 2002, 27(1-2):21-26.
66. Wu JY, Prentice H: Role of taurine in the central nervous system. J Biomed Sci 2010, 17 Suppl 1:S1.
67. Gertz M, Nguyen GT, Fischer F, Suenkel B, Schlicker C, Franzel B, Tomaschewski J, Aladini F, Becker C, Wolters D et al: A molecular
mechanism for direct sirtuin activation by resveratrol. PLoS One 2012, 7(11):e49761.
68. Mohar DS, Malik S: The Sirtuin System: The Holy Grail of Resveratrol? J Clin Exp Cardiolog 2012, 3(11).
69. Dean O, Giorlando F, Berk M: N-acetylcysteine in psychiatry: current therapeutic evidence and potential mechanisms of action. J
Psychiatry Neurosci 2011, 36(2):78-86.
70. Farr SA, Poon HF, Dogrukol-Ak D, Drake J, Banks WA, Eyerman E, Butterfield DA, Morley JE: The antioxidants alpha-lipoic acid and N-
acetylcysteine reverse memory impairment and brain oxidative stress in aged SAMP8 mice. J Neurochem 2003, 84(5):1173-1183.
71. Thakurta IG, Chattopadhyay M, Ghosh A, Chakrabarti S: Dietary supplementation with N-acetyl cysteine, alpha-tocopherol and alpha-
lipoic acid reduces the extent of oxidative stress and proinflammatory state in aged rat brain. Biogerontology 2012, 13(5):479-488.
72. Liu J: The effects and mechanisms of mitochondrial nutrient alpha-lipoic acid on improving age-associated mitochondrial and cognitive
dysfunction: an overview. Neurochem Res 2008, 33(1):194-203.

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