This document discusses research on the use of black soldier fly meal as a feed ingredient for Atlantic salmon. It summarizes research from the Institute of Marine Research in Norway on the nutritional qualities and safety considerations of black soldier fly meal. The document outlines the protein, lipid and amino acid composition of black soldier fly larvae and how it compares to Atlantic salmon requirements. It also discusses research trials feeding black soldier fly meal to Atlantic salmon to evaluate its effects on growth performance, nutrient digestibility, fillet quality, and stress response in fish.
Insects as PROTEIN SOURCE IN POULTRY
Introduction
Insects as a alternative feed
Type of insects
Insect farming
Nutritional value of insets and functional properties
Feeding value in different sps of animals
Risk profile and major concerns
Cost economics and environmental foot print
Future research
Conclusions and recommendations
This presentation is about using sustainable fly farming and processing techniques to get commercially viable protein source as fish and soy meal alternatives.
Small-scale farming of Edible Insects & Potential Contributions to Community ...Thomas Weigel
This presentation was held at AIDF's Asia Food Security Summit 2014 in Jakarta. It takes a look at edible insect farming from a food and nutrition security perspective and Veterinarians without Borders' (VWB) work on insects in Laos.
Farming of edible insects has big potential to ease the double burden of poverty and malnutrition. In many countries people love insects, and farmed insects are an excellent source of valuable protein, fats, and micro-nutrients. Insect farming is easy to learn, requires minimal time and money, and provides food for families. Insect farming has also significant advantages over the collection of wild insects in terms of improved availability, accessibility, and utilization of insects.
Environmentally sustainable, insects have a much better feed conversion and produce significantly less greenhouse gases than conventional livestock. Moreover, the sales of insects and insect products can provide additional income for poor people.
VWB has launched 2 cricket farming projects in Central Laos, involving a total of 36 households in two provinces, working mostly with women household members.
VWB's action-research approach involves the support of farmers to improve family diets, income, and also value-added foods such as cricket noodles. VWB is also studying the impact of cricket farming on child and maternal nutrition.
Insects as PROTEIN SOURCE IN POULTRY
Introduction
Insects as a alternative feed
Type of insects
Insect farming
Nutritional value of insets and functional properties
Feeding value in different sps of animals
Risk profile and major concerns
Cost economics and environmental foot print
Future research
Conclusions and recommendations
This presentation is about using sustainable fly farming and processing techniques to get commercially viable protein source as fish and soy meal alternatives.
Small-scale farming of Edible Insects & Potential Contributions to Community ...Thomas Weigel
This presentation was held at AIDF's Asia Food Security Summit 2014 in Jakarta. It takes a look at edible insect farming from a food and nutrition security perspective and Veterinarians without Borders' (VWB) work on insects in Laos.
Farming of edible insects has big potential to ease the double burden of poverty and malnutrition. In many countries people love insects, and farmed insects are an excellent source of valuable protein, fats, and micro-nutrients. Insect farming is easy to learn, requires minimal time and money, and provides food for families. Insect farming has also significant advantages over the collection of wild insects in terms of improved availability, accessibility, and utilization of insects.
Environmentally sustainable, insects have a much better feed conversion and produce significantly less greenhouse gases than conventional livestock. Moreover, the sales of insects and insect products can provide additional income for poor people.
VWB has launched 2 cricket farming projects in Central Laos, involving a total of 36 households in two provinces, working mostly with women household members.
VWB's action-research approach involves the support of farmers to improve family diets, income, and also value-added foods such as cricket noodles. VWB is also studying the impact of cricket farming on child and maternal nutrition.
Black Soldier Fly: The Star of Insect Farming
The Black Soldier Fly (BSF), Hermetia illucens (L.), is a wasp-like fly from the Stratiomyidae family of the order Diptera. It is native to the tropical regions of South America and is found globally in tropical and warm temperate regions between latitudes 45°N and 40°S. The insect completes its life cycle in around 25 days, consisting of four developmental stages: egg, larva, pupa, and adult. The larvae undergo six instars, where the final instar develops into a mobile, prepupa.
BSF larvae are commercially mass produced for various purposes, viz., fish and livestock feed, organic waste management, chitin production, bioplastic manufacturing, compost preparation, and many other commercial uses. BSF can also be consumed as human feed in a fried or salted state. BSF prepupae are reported to constitute around 36% to 65% of protein content and 4% to 38% of crude fat content, along with various aminoacids and micronutrients. The prepupal stage of BSF has an average nitrogen, phosphorous, and potassium content of 3.26%, 0.98%, and 1.03%, respectively.
The larval stage of BSF is capable of bioconversion of organic wastes such as animal faeces, kitchen waste, vertebrate remains and decompose them into homogeneous substrate in a shorter time period as compared to vermicompost production. Depending on the size and stage of the larvae, type of the substrate available and environmental conditions, the larvae consume around 25 to 500 mg of organic matter per larva on daily basis (Kim et al. 2021). Larvae can be easily mass produced at farm level with minimal space requirements and its prepupae are used as a perfect substitute for the expensive soybean meal and fish meal diets.
The BSF larval frass and its residues are applied as organic fertiliser, which improves the growth and development of plants (Lopes et al. 2022). By diverting organic waste to BSF larvae, the waste is efficiently converted into biomass, significantly reducing methane and other greenhouse gas emissions. Chitin can be produced from the BSF pupal shells, which has many applications in agriculture, textiles, and the pharmaceutical industry. BSF-derived oil has a high concentration of medium-chain saturated fatty acids (27% to 50% total fatty acids), which makes it potentially an ideal substrate for producing high-quality biodiesel. The lactic acid fermented products of BSF possess a high antimicrobial biomass, which inhibits pathogens like Salmonella enterica and Escherichia coli.
Due to its fast growth, reproduction, and ease with which it can be maintained, the BSF has gained recognition as a highly promising insect for farming and thus earned its reputation as the "Star of Insect Farming". BSF farming can be particularly effective in regions with limited access to traditional protein source, which can improve food and nutritional security. Moreover, India’s climate is well suited for BSF.
An engineered BSF processing facility can be designed and operated to achieve certain target objectives based on the natural life cycle of BSF. These, for instance, can be to cost effectively augment larvae quality or maximize the larval mass quantity produced within a certain time frame or
based on a particular feedstock, similar to a typical livestock rearing system (chicken, beef, etc.).
Entomophagy to address malnutrition & food insecurityDileepKC
Entomophagy to address malnutrition & food insecurity. Entomophagy literally means the consumption of insect by humans. It is a term derived from
the Greek word entomos (insect) and phagein (to eat), the practice is a well-established
although a diminishing custom of many parts of the world
Entomophagy is the practice of eating insects as food. Entomophagy is found in different taxonomic groups. There are many insects, birds, reptiles, amphibians and mammals that benefit from eating insects.
"Because insects possess physical and sensory characteristics that make them unattractive as food in Western culture, methods for extraction of protein for addition to other food have been tested, to increase their acceptance".
Black Soldier Fly: The Star of Insect Farming
The Black Soldier Fly (BSF), Hermetia illucens (L.), is a wasp-like fly from the Stratiomyidae family of the order Diptera. It is native to the tropical regions of South America and is found globally in tropical and warm temperate regions between latitudes 45°N and 40°S. The insect completes its life cycle in around 25 days, consisting of four developmental stages: egg, larva, pupa, and adult. The larvae undergo six instars, where the final instar develops into a mobile, prepupa.
BSF larvae are commercially mass produced for various purposes, viz., fish and livestock feed, organic waste management, chitin production, bioplastic manufacturing, compost preparation, and many other commercial uses. BSF can also be consumed as human feed in a fried or salted state. BSF prepupae are reported to constitute around 36% to 65% of protein content and 4% to 38% of crude fat content, along with various aminoacids and micronutrients. The prepupal stage of BSF has an average nitrogen, phosphorous, and potassium content of 3.26%, 0.98%, and 1.03%, respectively.
The larval stage of BSF is capable of bioconversion of organic wastes such as animal faeces, kitchen waste, vertebrate remains and decompose them into homogeneous substrate in a shorter time period as compared to vermicompost production. Depending on the size and stage of the larvae, type of the substrate available and environmental conditions, the larvae consume around 25 to 500 mg of organic matter per larva on daily basis (Kim et al. 2021). Larvae can be easily mass produced at farm level with minimal space requirements and its prepupae are used as a perfect substitute for the expensive soybean meal and fish meal diets.
The BSF larval frass and its residues are applied as organic fertiliser, which improves the growth and development of plants (Lopes et al. 2022). By diverting organic waste to BSF larvae, the waste is efficiently converted into biomass, significantly reducing methane and other greenhouse gas emissions. Chitin can be produced from the BSF pupal shells, which has many applications in agriculture, textiles, and the pharmaceutical industry. BSF-derived oil has a high concentration of medium-chain saturated fatty acids (27% to 50% total fatty acids), which makes it potentially an ideal substrate for producing high-quality biodiesel. The lactic acid fermented products of BSF possess a high antimicrobial biomass, which inhibits pathogens like Salmonella enterica and Escherichia coli.
Due to its fast growth, reproduction, and ease with which it can be maintained, the BSF has gained recognition as a highly promising insect for farming and thus earned its reputation as the "Star of Insect Farming". BSF farming can be particularly effective in regions with limited access to traditional protein source, which can improve food and nutritional security. Moreover, India’s climate is well suited for BSF.
An engineered BSF processing facility can be designed and operated to achieve certain target objectives based on the natural life cycle of BSF. These, for instance, can be to cost effectively augment larvae quality or maximize the larval mass quantity produced within a certain time frame or
based on a particular feedstock, similar to a typical livestock rearing system (chicken, beef, etc.).
Entomophagy to address malnutrition & food insecurityDileepKC
Entomophagy to address malnutrition & food insecurity. Entomophagy literally means the consumption of insect by humans. It is a term derived from
the Greek word entomos (insect) and phagein (to eat), the practice is a well-established
although a diminishing custom of many parts of the world
Entomophagy is the practice of eating insects as food. Entomophagy is found in different taxonomic groups. There are many insects, birds, reptiles, amphibians and mammals that benefit from eating insects.
"Because insects possess physical and sensory characteristics that make them unattractive as food in Western culture, methods for extraction of protein for addition to other food have been tested, to increase their acceptance".
We investigated the effects of fish protein hydrolysate (FPH) on zootechnical performance and immune response of the Asian Seabass Lates calcarifer Bloch. Experimental fish were fed with 3 diets: a local commercial diet (control), coated or not, with 2 and 3% FPH (w/w). Twelve thousand Asian Seabass juveniles (5.88±0.56 g) were divided into three groups and two replicates reared in nursery tanks (2000 L). The remaining fish were then used for grow-out experiment in floating net cages (1m x 1 m x 3 m). Zootechnical performances were assessed at both stages with following indicators: total weight gain (TWG), % relative weight gain (% RWG), % specific growth rate (% SGR), final weight (g) and final length (cm). At the end of each trial period, fish immune status was assessed through blood sampling and the measurement of Neutrophile (%), Monocyte (%), Lymphocyte (%), Macrophage (105 cell/mL), Leukocyte (103 cell/mL) and Phagocytes activity (%). At the end of the nursery trial, an immersion bacterial challenge with Vibrio parahaemolyticus (105 cells mL-1) was implemented. The results showed that dietary FPH supplementation significantly influenced the growth and immune status of Asian Seabass when compared to the control group. Fish fed FPH supplemented diet yielded higher growth rates and survival rates than non supplemented group. Fish phagocytic activity and resistance to a bacterial challenge were also improved by dietary FPH supplementation. These results may be related to the significant changes observed in fish leukocyte profiles, when fed FPH supplemented diets. Altogether, these results show the positive contribution of FPH to the sustainability of Asian seabass farming.
Visceral organ of colored broiler chicken (Gallus domesticus) fed with commer...Innspub Net
This study was conducted atcmU Poultry Production Project, Musuan, and Maramag Bukidnon to evaluate the effect of Black Soldier Fly Larvae (Hermetia illucens) under a free-range condition in the commercial ration on the visceral organ of colored broiler chicken. A total of 12 birds were obtained from a flock of 48 colored broiler chickens from a growth performance study. The treatments were as follows: Treatment 1 = 100g commercial feeds (control), Treatment 2 = 95% commercial feeds + 5% BSF larvae, Treatment 3 = 90% commercial feeds + 10% BSF larvae and Treatment 4 = 85% commercial feeds + 15% BSF larvae. Based on the result of analysis of variance (ANOVA), it showed no significant differences among the parameters of the studied weights of the crop with and without fill, weight of proventriculus without fill, weight of small intestine with and without fill, large intestine with and without fill, caeca with and without fill, weight of heart, and weight of gall bladder. However, the weight of proventriculus plus gizzard with fill, weight of liver, weight of spleen, weight of pancreas revealed significant differences based on Duncan’s Multiple Range Test (DMRT). Furthermore, Black Soldier Fly Larvae (Hermetia illucens) were highly recommended to animals because there were no detrimental observed in the study. This result indicates that using Black Soldier Fly Larvae (Hermetia illucens) as supplementation ration can improve the visceral organ performance of broiler chicken.
Research Inventy : International Journal of Engineering and Scienceresearchinventy
Research Inventy : International Journal of Engineering and Science is published by the group of young academic and industrial researchers with 12 Issues per year. It is an online as well as print version open access journal that provides rapid publication (monthly) of articles in all areas of the subject such as: civil, mechanical, chemical, electronic and computer engineering as well as production and information technology. The Journal welcomes the submission of manuscripts that meet the general criteria of significance and scientific excellence. Papers will be published by rapid process within 20 days after acceptance and peer review process takes only 7 days. All articles published in Research Inventy will be peer-reviewed.
The IOSR Journal of Pharmacy (IOSRPHR) is an open access online & offline peer reviewed international journal, which publishes innovative research papers, reviews, mini-reviews, short communications and notes dealing with Pharmaceutical Sciences( Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy & Phytochemistry, Pharmacology, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest........more details on Aim & Scope).
All manuscripts are subject to rapid peer review. Those of high quality (not previously published and not under consideration for publication in another journal) will be published without delay.
The development of aquafeed production is followed by the growing interest in raw materials which are to be interesting, attractive and valuable, not only in terms of their properties. There is no doubt that algae are one of them. Feeds with the addition of algae are perceived as premium products. This can result from the fact that algae evoke certain associations with healthy food for humans. Animal food with algae must then trigger the same positive associations. Moreover, specially processed algae or feeds with the addition of algae offered by the producers allowed for keeping popular algae-eating freshwater and marine fish.
The aquaculture industry has developed significantly over recent decades and is, today, one of the fastest-growing food production sectors in the world. One of the most important problems that affect aquaculture is the appearance of infectious diseases. Among bacterial diseases affecting cultured salmonid fish, Bacterial Cold-Water Disease, caused by Flavobacterium psychrophilum, produces high mortality and morbidity and consequently, economical losses worldwide.
Use of Silage Acid Devil Fish (Pterygoplichthys spp.) as Protein Supplement i...criollito
Animal nutrition is one of the most important limiting factors in animal production, especially in ruminants, providing
proteins being the main constraint, due to the limited availability and high cost of protein sources (oilseed meals). Currently in the dam
“El Infiernillo” in Michoacán México, has a large population of devil fish (Pterygoplichthys spp.), which is an economic and ecological
problem, because it is not consumed by humans and causes pollution to be discarded directly into the environment. For that reason the
objective of this study was to evaluate the use of silage acid devil fish (SADF) in fattening beef cattle as a protein supplement. SADF is
defined as a product semi-liquid or pasty mixed with formic acid, which leads to a decrease in pH to near 4.0. Used 18 young bulls (Bos
taurus × Bos indicus) for 60 days with a starting weight of 278.9 ± 51.2 kg, housed in individual pens with food and water ad libitum
were randomly assigned to three treatments with different levels of inclusion SADF (0%, 12% and 18%). They were weighed to the
beginning of the experiment and later every 30 days, previous fasting of 24 hours. To determine the food consumption, weigh every day
the offered food and the surplus. There were no significant differences (P < 0.05) among treatments with different levels of inclusion of
SADF with respect to daily weight gain, with values of 952 ± 324, 927 ± 322 and 854 ± 307 g/day, respectively. The dry matter intake
(DMI) was 8.9, 9.3 and 7.7 kg/day to 0%, 12% and 18% of SADF, respectively. In the same values for feed conversion were 9.34, 10.03
and 9.01 kg DMI/kg of weigh live, and carcass yield of 60.6%, 60.3% and 58.5%, respectively. It is concluded that fish silage acid devil
is an excellent alternative in feeding beef cattle as a protein supplement.
Soy protein concentrate: a value- added soy product for aquafeedsCJBio3
Aquaculture is the world's fastest-growing industry in the food production sector. It is projected that aquaculture will play a major role in the global food supply by doubling and intensifying its production by 2050. Fish meal is considered as “the gold standard” ingredients for aqua feed, but its unstable price and availability might decelerate the sector expansion and hurt famer’s profits. Although plant-based ingredients can be fish meal alternatives, their high content of antinutritional factors might cause some negative effects on fish growth. However, soy protein concentrate, which provides a great source of protein with less antinutrients thanks to the aqueous alcohol extraction in its refining process, are employing widely for better cost management with stable feed quality.
The article today will provide some updates about SPC using in feed production and the benefits which it brings on fish growth performance.
The study was carried out to determine the effect of fungi contaminated feed on the growth and survival of catfish, Clarias gariepinus juveniles. This research was carried out for a period of twelve weeks. Forty catfish juveniles were stocked at a rate of twenty juveniles per plastic tank. Catfish juveniles in one tank were fed with moldy feed and the control was served with non -moldy feed and was observed for twelve weeks to determine and compare their growth and survival. Catfish juveniles fed with moldy feed had the highest mortality as well as slower growth as compared to the control fed with non-moldy feed. The survival rate of juveniles stocked was 55% and mortality rate was 45% and majority of mortality was from juveniles fed with moldy feed and majority of the survival rate was from juveniles fed with non-moldy feed. Some water quality parameters such as temperature, dissolved oxygen and pH were also taken and no significant difference was observed. Moldy feed or feedstuff should not be used as this can cause great mortality and therefore loss to fish farmers.
Effects of dietary thyme essential oil on hemato-immunological indices, intes...Inacio Mateus Assane
Thyme (Thymus vulgaris) essential oil (TVEO) is a herbal medicine with one of the highest levels of antimicrobial activity. Although TVEO has been broadly used in poultry and swine production due to its immunostimulatory and growth-promoting characteristics, the effects of TVEO on fish are poorly characterized. In this study, Nile tilapia (Oreochromis niloticus L.) were fed 0, 0.1, 0.5, and 1% TVEO for 15 days. Subsequently, blood parameters, intestinal morphology, and the population of Bacillus bacteria in the intestine were evaluated. The numbers of lymphocytes (p < 0.05) and leukocytes (p < 0.05) significantly increased in the blood of the fish fed the highest dose of TVEO. Based on the normal behavior of the fish and the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, which were not altered (p > 0.05), this study concluded that the diets were safe and showed no negative or toxic effects. Even at doses as high as 1%, TVEO did not alter the population of beneficial Bacillus bacteria in the gut. In conclusion, supplementation with TVEO stimulated the cellular components of the non-specific immune response of Nile tilapia without causing deleterious effects or altering the population of important intestinal bacteria.
Similar to Black soldier fly meal in feed for Atlantic salmon - Erik-Jan Lock, Havforsknings Instituttet (20)
Luken webinaarissa kerrotaan, mitkä ovat Ukrainan sodan akuutit vaikutukset Suomen ruokamarkkinoilla sekä metsäsektorilla ja miten sota vaikuttaa pitkällä aikavälillä vihreän siirtymän toteutumiseen.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
THE IMPORTANCE OF MARTIAN ATMOSPHERE SAMPLE RETURN.Sérgio Sacani
The return of a sample of near-surface atmosphere from Mars would facilitate answers to several first-order science questions surrounding the formation and evolution of the planet. One of the important aspects of terrestrial planet formation in general is the role that primary atmospheres played in influencing the chemistry and structure of the planets and their antecedents. Studies of the martian atmosphere can be used to investigate the role of a primary atmosphere in its history. Atmosphere samples would also inform our understanding of the near-surface chemistry of the planet, and ultimately the prospects for life. High-precision isotopic analyses of constituent gases are needed to address these questions, requiring that the analyses are made on returned samples rather than in situ.
This pdf is about the Schizophrenia.
For more details visit on YouTube; @SELF-EXPLANATORY;
https://www.youtube.com/channel/UCAiarMZDNhe1A3Rnpr_WkzA/videos
Thanks...!
Deep Behavioral Phenotyping in Systems Neuroscience for Functional Atlasing a...Ana Luísa Pinho
Functional Magnetic Resonance Imaging (fMRI) provides means to characterize brain activations in response to behavior. However, cognitive neuroscience has been limited to group-level effects referring to the performance of specific tasks. To obtain the functional profile of elementary cognitive mechanisms, the combination of brain responses to many tasks is required. Yet, to date, both structural atlases and parcellation-based activations do not fully account for cognitive function and still present several limitations. Further, they do not adapt overall to individual characteristics. In this talk, I will give an account of deep-behavioral phenotyping strategies, namely data-driven methods in large task-fMRI datasets, to optimize functional brain-data collection and improve inference of effects-of-interest related to mental processes. Key to this approach is the employment of fast multi-functional paradigms rich on features that can be well parametrized and, consequently, facilitate the creation of psycho-physiological constructs to be modelled with imaging data. Particular emphasis will be given to music stimuli when studying high-order cognitive mechanisms, due to their ecological nature and quality to enable complex behavior compounded by discrete entities. I will also discuss how deep-behavioral phenotyping and individualized models applied to neuroimaging data can better account for the subject-specific organization of domain-general cognitive systems in the human brain. Finally, the accumulation of functional brain signatures brings the possibility to clarify relationships among tasks and create a univocal link between brain systems and mental functions through: (1) the development of ontologies proposing an organization of cognitive processes; and (2) brain-network taxonomies describing functional specialization. To this end, tools to improve commensurability in cognitive science are necessary, such as public repositories, ontology-based platforms and automated meta-analysis tools. I will thus discuss some brain-atlasing resources currently under development, and their applicability in cognitive as well as clinical neuroscience.
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Richard's aventures in two entangled wonderlandsRichard Gill
Since the loophole-free Bell experiments of 2020 and the Nobel prizes in physics of 2022, critics of Bell's work have retreated to the fortress of super-determinism. Now, super-determinism is a derogatory word - it just means "determinism". Palmer, Hance and Hossenfelder argue that quantum mechanics and determinism are not incompatible, using a sophisticated mathematical construction based on a subtle thinning of allowed states and measurements in quantum mechanics, such that what is left appears to make Bell's argument fail, without altering the empirical predictions of quantum mechanics. I think however that it is a smoke screen, and the slogan "lost in math" comes to my mind. I will discuss some other recent disproofs of Bell's theorem using the language of causality based on causal graphs. Causal thinking is also central to law and justice. I will mention surprising connections to my work on serial killer nurse cases, in particular the Dutch case of Lucia de Berk and the current UK case of Lucy Letby.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Unveiling the Energy Potential of Marshmallow Deposits.pdf
Black soldier fly meal in feed for Atlantic salmon - Erik-Jan Lock, Havforsknings Instituttet
1. Black soldier fly meal in feed for Atlantic salmon
- nutritional qualities and safety considerations -
Erik-Jan Lock, Irene Biancarosa, Ikram Belghit
2. Institute of Marine Research
• One of the largest Marine Research Institutes in Europe (~1000
employees)
• Main activities: research, advisory work and monitoring
3.
4. Institute of Marine Research
• One of the largest Marine Research Institutes in Europe (~1000
employees)
• Main activities: research, advisory work and monitoring
• Leading supplier of knowledge relating to the sustainable management of
the resources in our marine ecosystems and the whole food chain from the
sea to the table
8. Insect as feed ingredients
Black soldier fly (BSF) larvae
0
10
20
30
40
50
60
CP Arg His Ile Leu Lys Met Thr Val
Partially defatted BSF larvae
Atlantic salmon requirements (NRC, 2011)
Most of the indispensable amino acids are close to the requirements in Atlantic salmon
Amino acids composition= % of crude protein
Atlantic salmon requirements
10. Insect as natural feed ingredients for sustainable salmon
?
Evaluation of seaweed as a feed ingredients
for BSF larvae
Belghit I, Liland NS, Waagbø R, Biancarosa I, Pelusio N, Li Y, Krogdahl Å, Lock EJ. 2018. Potential of insect-based diets for
Atlantic salmon (Salmo salar). Aquaculture 491: 72-81
11. Increased EPA (retention around 35%)
Increased iodine
Increased tocopherols
N.S. Liland, I. Biancarosa, P. Araujo, D. Biemans, C.G. Bruckner, R. Waagbø, B.E. Torstensen, E.-J. Lock, Modulation of nutrient
composition of black soldier fly (Hermetia illucens) larvae by feeding seaweed-enriched media, PLOS ONE, 12 (2017)
I. Biancarosa, N.S. Liland, D. Biemans, P. Araujo, C.G. Bruckner, R. Waagbø, B.E. Torstensen, E.-J. Lock, H. Amlund, Uptake of heavy
metals and arsenic in black soldier fly (Hermetia illucens) larvae grown on seaweed-enriched media, J. Sci. Food Agric.
Black soldier fly larvae experimental trial
13. Insect as natural feed ingredients for sustainable salmon
?
Evaluation of insect meal (IM) as a feed
ingredients for Atlantic salmon
Belghit I, Liland NS, Waagbø R, Biancarosa I, Pelusio N, Li Y, Krogdahl Å, Lock EJ. 2018. Potential of insect-based diets for
Atlantic salmon (Salmo salar). Aquaculture 491: 72-81
14. Insect meal (IM)BSF larvae
FM/VP
Control diet (IM-0%)
66%IM
/VP
IM-66%
100%IM
/VP
IM-100%
Cargill
Replacement
60:40
Feed ingredients and experimental diets
Belghit et al
IM-33%
33%IM
/VP
Feeding period:16 weeks
WP2
Experiment 2. Grow-out trial
15. IM -0 IM -6 6IM -1 0 0 IM -0 IM -6 6IM -1 0 0 IM -0 IM -6 6IM -1 0 0
0
1 0 0 0
2 0 0 0
3 0 0 0
4 0 0 0
5 0 0 0
Grwoth(g)
Main results
• 3 fold increase in body weight
• No significant effects on growth
between groups
• No effects on nutrient digestibility
• Whole body FA composition was
affected by IM dietary inclusion
0 33 66 100 0 33 66 100 0 33 66 100
Belghit et al., submitted
WP2
Experiment 2. Grow-out trial
16. Head Kidney (HK) Isolated leukocytes
+ LPS
+ Poly I:C
+ Control (non treatment)
BSF insect meal (IM)
IM-0%
IM-66%
IM-100%
Gene expression
3rd October ≈2500 g
54 days
7th August ≈1200 g Isolated for 24h and added
treatment for additional 24h
2 fish/ cages = 6 fish/diet
IM -0 IM -6 6IM -1 0 0 IM -0 IM -6 6IM -1 0 0 IM -0 IM -6 6IM -1 0 0
0
1 0 0 0
2 0 0 0
3 0 0 0
4 0 0 0
5 0 0 0
Grwoth(g)
0 33 66 100 0 33 66 100 0 33 66 100
WP2
Experiment 2. Grow-out trial
Inflammatory related gene expression
Eicosanoid related gene expression
Stress related gene expression
17. Main results
WP2
Experiment 2. Grow-out trial
• Replacement of fishmeal with IM in the diets of Atlantic
salmon had no effect on the transcription of pro-
inflammatory genes in the head kidney cells.
• Fish fed with IM had down regulated transcription of
antioxidant and stress related genes.
IM - 0 IM - 66 IM - 100
0 .0
0 .2
0 .4
0 .6
0 .8
1 .0
S O D
¤
z
y
y
IM - 0 IM - 66 IM - 100
0 .0
0 .2
0 .4
0 .6
0 .8
1 .0
5 -LO X
z
y y
In vitro bacterial and viral response in head kidney leukocytes of Atlantic
salmon (Salmo salar) fed dietary insects meal
18. Sensory testing
• Softer texture of the raw fillets in fish
fed 100% IM than fish fed 33 % IM
• Higher rancidity odor of baked fillet
from fish fed 100% IM than the
control group
• No differences in color scores of raw
fish
Belghit et al., submitted
WP2
Experiment 2. Grow-out trial
19. Insect as feed ingredients for Atlantic salmon
Christopher Haatuft
20. -> Must comply with the associated legal constraints to
guarantee their safe use in fish feed ingredients
+ Large variety in origin and quality of insect products
Objective
Analytical approaches are urgently required for a proper detection
and identification of insect species-containing feed ingredients
ENTOFÔR project - From waste to resource
21. Insect meal (IM)
- 18 commercial IM samples
- Different companies - Different processing:
whole larvae meal or defatted insect meal
8 Black soldier fly (Hermetia illucens) (BSF)
5 Yellow mealworm (Tenebrio molitor) (YM)
3 Lesser mealworm (Alphitobius diaperinus) (LM)
2 House cricket (Acheta domesticus) (HC)
Materials
22. The protein content of the 18 IM samples
BSF YM LM HC
True protein content was determined as sum of amino acid residue
%oftotalAAAmino acid composition, protein content, and nitrogen-to-protein conversion factors of 21 seaweed species from Norwegian waters,
Biancarosa et al., 2016
0
2 0
4 0
6 0
8 0
1 0 0
0
2 0
4 0
6 0
8 0
1 0 0
Crude protein from overall nitrogen content, using the traditional
nitrogen-to-protein conversion factor of 6.25
BSF YM LM HC
Crudeprotein
23. Principal component analysis and hierchical clustering of the amino acids calculated
from 18 insect meal samples
BSF
HC
LM
YM
His
Glu
Met
Asp
Phe
Lys
Thr
Tau
Arg
Tyr
Iso
Ser
Pro
Ala
Val
Gly
Leu
BSF
YMLM
HC
BSF
25. PeptidesProteins extraction
Peptidomic profiling of different (commercially) available insect species
LC-MS/MS Spectral
Species Protein Peptides
BSF 1
21 53
BSF 2
43 196
BSF 3
21 103
BSF 4
81 275
BSF 5
18 91
BSF 6
58 303
BSF 7
56 156
BSF 8
55 220
YW 9
127 765
YW 10
138 689
YW 11
133 697
YW 12
54 235
YW 13
130 664
LW 14
94 509
LW 15
90 477
LW 16
94 389
HC 17
77 605
HC 18
75 454
Name Common names
Number of entries
(UNIPROT)
Number of proteins
(NCBI)
ACHETA DOMESTICUS House cricket 159 168
HERMETIA ILLUCENS Black soldier fly, BSF 56 348
TENEBRIO MOLITOR Yellow mealworm, beetles 527 607
ALPHITOBIUS
DIAPERINUS
Lesser mealworms,
buffalo worms, litter
beetles
27 63
ZOPHOBAS MORIO or
ZOPHOBAS ATRATUS
or ZOPHOBAS
RUGIPES
Morio worms, super
worm, king worm
19 38
26. PeptidesProteins extraction
Peptidomic profiling of different (commercially) available insect species
LC-MS/MS Spectral
All tandem mass spectra ≈ 4000
Pairwise comparaison CompareMS2
Records the similarity of the best matching spectrum in the corresponding range
Creating a distance matrix
Phylogenetic tree of the samples
27. Hierarchical Clustering of insect meal Proteomes
LM
LM
LM
YM
YM
YM
YM
YM
HC
HC
BSF
BSF
BSF
BSF
BSF
BSF
BSF
BSF
The phylogenetic analysis distinguished and successfully arranged the 18 different IM samples
species in groups of closely related families based on the comparison of 4000 tandem mass spectra+ increase -> spectral library data base
30. Developing peptide markers for the quantitative species specific
differentiation of insect meal
By using the gel-free shotgun proteomics approach in combination
with direct spectral comparison we were able to differentiate the
insect meal samples according to the taxonomic classification of the
insect species
Conclusion
By including IM in the diets, the concentration of lauric acid (12:0) increased (0.5-1.6% of the whole body total FA in the IM fed fish), while this FA was below the quantification limit in the whole fish fed diets without IM (IM0) (Table 6). The concentration of EPA and DHA as well as the n-3/n-6 ratio increased significantly with increasing inclusion of IM at 66% (IM66) and 100% (IM100) in the diets compared to fish fed with dietary IM0 and IM33. (Table 6).
The aim of the current study was to test how a partial or total replacement of dietary fishmeal with insect meal affect gene responses involved in inflammation and signaling pathways in Atlantic salmon (Salmo salar) head kidney leukocytes after exposure to bacterial or viral mimics.
bacterial mimic lipopolysaccharide (LPS) or viral mimic polyinosinic acid: polycytidylic acid (poly I: C) to induce an inflammatory response.
Different FA composition in the diets; migth have have changed the composition of cell membrane?
Have the FA profile in IM based diet caused changes in leukotriene production?
Less oxidative stress in fish given IM based diet?
Spider web diagram of the sensory evaluation of (A) raw and (B) baked fillets from IM0, IM33, IM66 and IM100 fed Atlantic salmon. A 100% substitution of fishmeal with insect meal (IM100) resulted in a softer texture of the raw fillets compared with the fillets of fish fed with only 33% fishmeal replacement (IM33). The rancidity odor of baked fillet from the IM100 group was significantly higher than in the control group without insect meal (IM0). p < 0.05; (one-way ANOVA). IM0 = diet without insect meal (IM) inclusion; IM33, IM66 and IM100 = 33, 66 and 100% replacement level of FM with IM, respectively.
Increasing number of insect-rearing farm for commercial purposes both inside and outside Europe
At least 26 companies in the world Example: ALPARE in Colombia produce BSF larvae 6 tons of IM/day
To demonstrate the feasibility of such a method we attempted to reconstruct a phylogenetic tree for humans and our closest living relatives based solely on uninterpreted tandem mass spectrometry data.