The document describes the Lightning-Link Biotin Conjugation Kit, which allows for the rapid conjugation of biotin to antibodies or other biomolecules. The kit contains a lyophilized mixture that activates upon the addition of the biomolecule to be labeled. This results in the directional coupling of biotin to the biomolecule in a gentle process at near-neutral pH. The conjugation can be performed on quantities ranging from micrograms to milligrams of the biomolecule. No separation steps are required after the conjugation is complete. Common buffer conditions that are compatible with the kit are listed.