The document describes a new coupling kit from Anteo Diagnostics that provides everything needed to couple proteins to 1 μm magnetic particles in a one-step reaction. The kit offers benefits like greater sensitivity, reproducibility and reduced development times compared to standard coupling methods. It contains pre-activated magnetic particles, buffers and microcentrifuge tubes, and allows coupling of proteins like peptides, antigens and antibodies in under two hours via a simple mixing process. The coupled particles are then compatible with fluorescent and chemiluminescent assays.

1. Coupling Kit
1 μm Magnetic Particles
Now Available
One-Step Protein Coupling
The AMG Difference
Greater sensitivity, increased reproducibility and
decreased development times are just some of
the benefits Mix&Go™ provides – all in a ready-to-
use, pre-activated particle format and
Coupling Kit
Performance:
Binding Capacity:
delivered in the new AMG (Anteo Mix&Go)
Coupling Kit. The kit includes everything you
need to enable fast and easy binding of proteins
to 1 μm magnetic particles in a one-step reaction.
The kit is suitable for coupling a broad range of
biomolecules including; peptides, antigens and
antibodies. Coupled particles are compatible with
a range of fluorescent and chemiluminescent
assays.
Mix&Go Coupling Efficiency
Mix&Go coupling efficiency is comparable to
standard covalent coupling without the handling
limitations users experience with covalent
coupling such as chemical instability and multiple
steps impacting on particle dispersity.
Fast, Easy and Hassle Free Conjugation
In one-step you can bind your protein of interest to the pre-activated 1 μm magnetic particles using the
AMG Coupling Kit (as illustrated below). It’s a fast, easy and user-friendly procedure that will let you get
on with the research that matters.
Mix&Go™
activated
particles
Bare
TOSYL
particles
Weigh out,
add EDC
30 min
room temp
Coupling Kit
Fast & Easy
One-Step Coupling
Bare
COOH
particles
Antibody
1 hr room
temp
Antibody
16-24 hr
incubation
at 37oC
Antibody
2-16 hr
incubation
Wash
Storage
Buffer
Wash
Storage
Buffer
Wash
Storage
Buffer
Monodispersity:
Kit components:
• Activated 1 μm
Magnetic Particles
• Coupling Buffer
• Blocking Buffer
• Storage Buffer
• Microcentrifuge
tubes
Mix&Go™
antibody
coupled
particles
Tosyl
antibody
coupled
particles
Amide
antibody
coupled
particles
> 20 μg IgG/mg
> 90%
Mix&Go™
total coupling
time < 2 hr
Tosyl
total coupling
time > 16 hr
Amide
total coupling
time: up to
16 hr
Anteo Diagnostics, Ltd. Unit 4, 26 Brandl Street, Brisbane Technology Park, Eight Mile Plains, QLD 4113, Australia
V1.0 07/14
Anteo Cat #: A-SMPCKMP-10 (1 mL; 10 mg/mL)
A-SMPCKMP-30 (3 mL; 10 mg/mL)

2. Coupling Kit
1 μm Magnetic Particles
Anteo Cat #: A-SMPCKMP-10 (1 mL; 10 mg/mL)
A-SMPCKMP-30 (3 mL; 10 mg/mL)
Experience the Mix&Go™ Advantage Today
• Simplified Handling
• Excellent Binding Capacity
• High Reproducibility
• High Superparamagnetic
• Highly Monodispersed
• Excellent Colloidal Stability
V1.0 07/14
50,000
45,000
40,000
35,000
30,000
25,000
20,000
15,000
10,000
5,000
0
MFI
Mix&Go Condition and Chemical
Compatibility during Coupling
Loading Assay: Mouse IgG - GAM-RPE
No Particle Activation Required
AMG Coupling Kits employ Anteo’s novel Mix&Go
proprietary surface coating.
The elimination of an activation step and pre-treatment
of proteins allows users to design and
generate novel and complex particle conjugates
by incorporating more than one protein in co-coupling
reactions.
Product Highlights:
Functionality
Exceptional Stability
Activated particles can withstand the following
conditions:
• Temperature: 1 hr at 60°C
• Harsh chemical conditions: 1% Tween-20,
50% DMSO, 4M Urea.
Protein coupled particles are stable under:
• pH extremes: pH 2- 10
• Temperature: 1 hr at 60°C
• Harsh Chemical treatments: ≤5% Tween20,
≤0.05M EDTA, ≤1M NaCl, ≤8M Urea, ≤50%
DMSO
The Science Behind Mix&Go
The surface chemistry incorporated into this
product provides a novel approach for attaching
fragile proteins to synthetic surfaces that is
distinct to the traditional methods of passive
adsorption and covalent binding. An aqueous
cationic polymer, Mix&Go, forms functional
protein mono-layers that are highly stable.
Anteo Diagnostics has a suite of application
specific reagents specifically formulated for
improved performance on a range of synthetic
surfaces. The AMG product line features products
incorporating the Mix&Go chemistry.
Contact
To place an order or to learn more;
• email sales@anteodx.com
• visit www.anteodx.com
Compatibility of AMG activated particles during
coupling. AMG activated particles were either stress
tested for 1 hr or coupled in the presence of the
indicated conditions. Performance was assessed by
binding to mouse IgG and assayed for loading capacity
with goat anti-mouse IgG-RPE.
60,000
50,000
40,000
30,000
20,000
10,000
0
Anteo Diagnostics, Ltd. Unit 4, 26 Brandl Street, Brisbane Technology Park, Eight Mile Plains, QLD 4113, Australia
MFI
Plate Fluorescence Loading Assay
x-RPE (as indicated)
The AMG Coupling Kit has broad applicability and can
be used to couple a range of proteins of different size.
Actual loading will vary depending on the protein
coupled, the size of the protein and the quantity of
protein added to the coupling reaction. Abbreviations;
mouse (mo), goat anti-mouse (GAM), rabbit (rb), goat
anti-rabbit (GAR), human (hu) and goat anti-human
(GAH).