This document provides an introduction and overview of biohazard and biorisk assessment. It discusses key topics including the definition of a biohazard, the purpose of biorisk assessment to ensure safe handling of biological materials, and the significance of risk assessment in scientific work to systematically identify and evaluate potential hazards. The document also covers considerations for biological, chemical, and animal work including common hazards, necessary risk assessment steps, and safety procedures. It emphasizes the importance of risk assessment, personnel training, and continuous education to adapt to biosafety challenges.
The document provides a detailed overview on the basic principles of operating a biotech or micro laboratory along with basic techniques with which to handle organisms, chemicals &equipment and ensuring your own, your colleagues and your environment's safety.
Biohazards,Institutional Biosafety Committees and Cartagena Protocol:
Biohazards:
Biological hazards also known as biohazards, refer to biological substances that pose a threat to the health of living organisms, especially that of humans. For example: Viruses, bacteria ,fungi etc.
These hazards can be encountered anywhere in the environment. The biohazard symbol was developed in 1966 by Charles Baldwin, an environmental health engineer.
Types of Biological Hazards: Biological hazards can be put into different categories:
Bacteria: microscopic organisms that live in soil,water or the bodies of plants and animals and are characterized by lack of distinct nucleus and the inability to photosynthesize. Examples are E.coli, TB and Tetanus.
Viruses: These are a group of pathogens that consist mostly of nucleic acids and that lack cellular structure. Viruses are totally dependent on their hosts for replication. Examples: common cold, influenza, measles, SARS, Hantavirus and rabies.
Fungi: Major group of lower plants that lack chlorophyll and live on dead or other living organisms. Examples: mould,rust, mildew,smut,yeast and mushrooms.
Biohazard Classification: Conventional Agents
Recombinant DNA
Tissue Culture
Animal work
Anatomical Specimens
Unconventional Agents
What is Biosafety ? Biosafety is the application of safety precautions that reduce a laboratorians risk of exposure to a potentially infectious material and limit contamination of the work environment and ultimately the community (CDC).
Achieved through;
Administrative controls
Engineering controls
Personal protective equipment
Practices and procedures
Institutional Biosafety Committee (IBC): Under section 5 (1) of regulations
All organisations involved in research and development that deals with modern biotechnology shall establish an IBC.
IBC is a formal expert committee of an organisation undertaking modern biotechnology research and development which involves use of any LMO/rDNA materials.
IBCs are registered with the National Biosafety Board (NBB).
Its function is to monitor and ensure compliance to the biosafety act 2007 at the institutional level and safe handling of modern biotechnology activities.
IBC Members: Head of the organization or his designate as the chairperson.
Three or more scientists engaged in rDNA work or molecular biology with at least one outside expert in the relevant discipline.
A member with medical qualifications - Biosafety officer.
A nominee of DBT.
Cartagena Protocol: History: CBD opened for signature in 1992 and entered into force on 29 Dec 1993.
Cartagena Bio Safety Protocol (CBSP) negotiated from 1996-2000; entered into force in 11 Sept. 2003; over 170 Party Members; an international treaty.
This is a complementary agreement to the United Nations Convention on Biological Diversity (CBD).
Total parties to the cartagena protocol as of June 2021 are 173.
Objectives: The cartagena protocol on Biodiversity seeks to protect biodiversity from the potential risk
The need of proper hospital
Waste management system is of prime importance and is an essential component to prevent spread and transmission of infections in Hospital.
So, this slide will give an overview for understanding Biomedical waste management.
The very first requirement in a hospital that it should do the sick no harm" - Florence Nightingale
Health care associated infections economic loss, prolonged hospital stay & adverse patient outcomes.
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The document provides a detailed overview on the basic principles of operating a biotech or micro laboratory along with basic techniques with which to handle organisms, chemicals &equipment and ensuring your own, your colleagues and your environment's safety.
Biohazards,Institutional Biosafety Committees and Cartagena Protocol:
Biohazards:
Biological hazards also known as biohazards, refer to biological substances that pose a threat to the health of living organisms, especially that of humans. For example: Viruses, bacteria ,fungi etc.
These hazards can be encountered anywhere in the environment. The biohazard symbol was developed in 1966 by Charles Baldwin, an environmental health engineer.
Types of Biological Hazards: Biological hazards can be put into different categories:
Bacteria: microscopic organisms that live in soil,water or the bodies of plants and animals and are characterized by lack of distinct nucleus and the inability to photosynthesize. Examples are E.coli, TB and Tetanus.
Viruses: These are a group of pathogens that consist mostly of nucleic acids and that lack cellular structure. Viruses are totally dependent on their hosts for replication. Examples: common cold, influenza, measles, SARS, Hantavirus and rabies.
Fungi: Major group of lower plants that lack chlorophyll and live on dead or other living organisms. Examples: mould,rust, mildew,smut,yeast and mushrooms.
Biohazard Classification: Conventional Agents
Recombinant DNA
Tissue Culture
Animal work
Anatomical Specimens
Unconventional Agents
What is Biosafety ? Biosafety is the application of safety precautions that reduce a laboratorians risk of exposure to a potentially infectious material and limit contamination of the work environment and ultimately the community (CDC).
Achieved through;
Administrative controls
Engineering controls
Personal protective equipment
Practices and procedures
Institutional Biosafety Committee (IBC): Under section 5 (1) of regulations
All organisations involved in research and development that deals with modern biotechnology shall establish an IBC.
IBC is a formal expert committee of an organisation undertaking modern biotechnology research and development which involves use of any LMO/rDNA materials.
IBCs are registered with the National Biosafety Board (NBB).
Its function is to monitor and ensure compliance to the biosafety act 2007 at the institutional level and safe handling of modern biotechnology activities.
IBC Members: Head of the organization or his designate as the chairperson.
Three or more scientists engaged in rDNA work or molecular biology with at least one outside expert in the relevant discipline.
A member with medical qualifications - Biosafety officer.
A nominee of DBT.
Cartagena Protocol: History: CBD opened for signature in 1992 and entered into force on 29 Dec 1993.
Cartagena Bio Safety Protocol (CBSP) negotiated from 1996-2000; entered into force in 11 Sept. 2003; over 170 Party Members; an international treaty.
This is a complementary agreement to the United Nations Convention on Biological Diversity (CBD).
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Objectives: The cartagena protocol on Biodiversity seeks to protect biodiversity from the potential risk
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1. Biohazard and Biorisk assessment, with
biological/chemical/animal work risk assessment
• presented by: IQRA AKRAM (2022-ag-2062)
IMAMA NASIR (2022-ag-1179)
TAYYABA WALI (2022-ag-1981)
SARA REHMAN(2022-ag-1993)
SANA WAHEED(2022-ag-1987)
RIDA FATIMA(2017-ag-8424)
SAMAN TAHIRA(2021-ag-1780)
ARFA ZULFIQAR(2021-ag-1802)
2. Introduction to Biohazard and Biorisk assessment
Biohazard
• A biohazard refers to any biological substance that poses a threat
to human health or the environment.These substances can
include microorganisms (such as bacteria, viruses, fungi, and
parasites), toxins produced by living organisms, and biological
materials that may contain these harmful agents.
• Biorisk Assessment
• Biorisk assessment is a systematic process that evaluates and
manages potential risks associated with biological agents and
materials.it includes hazard identification,risk assessment and
implementation of measures to control and mitigate risks.
• Purpose of Biorisk assessment is to ensure safe handling of
biological materials and effective response to biohazard incidens
3. Significance of risk assessment in scientific work
• Risk assessment is crucial in scientific work as it
systematically identifies and evaluates potential
hazards, enabling researchers to mitigate risks
and ensure the safety of both the experimenters
and the environment.
• Additionally, it enhances the reliability of
experimental results by minimizing unforeseen
complications, thereby contributing to the overall
integrity and credibility of scientific research.
4. Biological work concideration
• Biological material that have a known
risk to people and environment:
• Microorganisms(bacteria,viruses and
protists)
• human blood tissues and body
fluids(human blood,serum,tissues and
faecal specimen). this can have cell lines
of human or primate origin that are
known to have infectious viral agents.
5. Necessity of Risk Assessment
• Biological hazard risk assessment is used to alert
the staff to the hazards of working with infectious
agent and to the need for developing proficieny in
the use of selected safe practices and
containment equipment.
• Successful control of hazards in lab also protect
persons not directly associated with the lab, such
as other occupants of the same building, and the
public.
6. Chemical Work Considerations
• Common Hazards
1. Chemical spills and leaks
2. Toxic fumes and gases
3. Fire and explosions
4. Exposure to hazardous
substances
7. Necessity of Risk Assessment
• Steps in Risk Assessment
1. Identify hazards
2. Assess the risks
3. Implement control measures
4. Monitor and review
5. Legal and Ethical Obligations
6. Emphasize the legal requirements and ethical
responsibility of employers to conduct risk
assessments.
• Benefits
a. Improved safety culture
b. Reduced accidents and injuries
c. Compliance with regulations
8. Animal work consideration:
Animal work risk assessment:
Risk assessment is a crucial aspect of working with animals in a laboratory
setting to ensure the safety of both the researchers and the animals. Here are some
key considerations for conducting a risk assessment:
Physical Risks: Identify potential physical risks associated with handling animals,
such as bites, scratches, or injuries from larger animals. Implement appropriate
safety measures, such as using personal protective equipment (PPE) like gloves and
safety glasses.
Zoonotic Diseases: Assess the risk of zoonotic diseases (diseases that can be
transmitted between animals and humans). Follow recommended vaccination
protocols for relevant diseases.
Implement good hygiene practices, including handwashing, to reduce the risk
of disease transmission.
12. Environmental Safety Procedures
• The environment health and safety center has implemented a comprehensive for the
management of hazardous materials
• Microbiological, Chemical laboratories and procedures involving microorganism and
chemicals pose special safety problems so the following policy and procedures must be
followed rigorously.
• Risk
• Warning signs
• Separate areas
• Protective clothing
• Safety Cabinent
• Decontamination
• Levels of containment
13. Cont...
• Risk: Pathogens and chemical should be handled carefully based on the biorisk they pose to staff,
general public and animal outside the laboratory.
• Warning signs: A dangerous hazard must be indicated by standard biological warning signs giving the
type and degree of risk.
• Separate areas: separate areas should be set aside for the preparation of media, holding of the
material and sterilization. Animal rooms must be segregated from laboratory and should separated
infected from non infected animals.
• Protection clothing
• Safety Cabinent: Laminar flow and
• Biosafety cabinent
• Decontamination: Sterilization, Disinfection
• Waste disposal
• Level of contaiment