for laboraotry workers, researchers,trainers of health worker, students and instructors

1. Laboratory Biosafety and Biosecurity

3. Contents:
1. Introduction
2. Risk assessment
3. Core requirements
4. Heightened control measures
5. Maximum containment measures
6. Transfer and transportation
7. Biosafety programme management
8. Laboratory Biosecurity

4. 1. Introduction
Laboratory Biosafety and Biosecurity?
• Biosafety: Containment principles, technologies and practices that are
implemented to prevent unintentional exposure to biological agents or their
inadvertent release.
• Biosecurity: Principles, technologies and practices that are implemented for
the protection and control of biological materials and/or the equipment, skills
and data related to their handling.
• Biosecurity Aims to prevent their unauthorized access, loss, theft, misuse,
diversion or release.
• Biosafety Aims to protect the lab workforce and unintentional exposures or
releases of pathogenic biological agents.

5. Research based Facts!
• A review of recent laboratory-associated infections showed that most were
caused by human factors rather than malfunctions of engineering
controls(4,5).
• Factors that have led to potential and confirmed exposures to biological
agents include:
 Absence or improper use of personal protective equipment (PPE) (6,7),
 Inadequate or ignored risk assessments (8),
 Lack of standard operating procedures (SOPs) (9),
 Needle stick injuries (10,11)
 Insufficiently trained personnel

6. 2. Risk assessment
• Control of biological risks - is informed by performing a risk assessment
• Risk defines the probability that a hazard will cause harm and the severity
of harm.
• Risk assessment is the term used to describe the stepwise process in
which the risk(s) arising from working with a hazard(s) are evaluated and
the resulting information is used to determine whether risk control
measures can be applied to reduce those risks to acceptable risks.

7. Continue…..
• Hazards??? are biological agents whose pathogenic characteristics give them
the potential to cause harm to humans or animals.
• Is hazards alone do pose a risk to humans or animals.????????
yes or No??
• Hazards alone do not pose a risk to humans or animals.
• For example, a vial of blood containing an Ebola virus…………
(True risk can't be determined only by knowing the pathogenic characteristics……..)

8. • Any facility that handles biological agents ……. perform a risk
assessment ….. risk control measures …….. to reduce those risks to an
acceptable risk.
• Analysis of RISKS empowers laboratory personnel in:
 Deeper understanding of the biological risks
 Importance of safety,
 Work safely
 Maintain a safety culture in the laboratory.

9. The Risk assessment framework

10. Key considerations in the risk assessment framework

14. Challenges may include:
• Level of organizational and financial resources available to manage biological
risks;
• Absence of a reliable electrical supply;
• Inadequate facility infrastructure;
• Severe weather;
• Under-staffed laboratories;
• Under-trained personnel.
Furthermore, a risk assessment and the risk control measures implemented may
vary considerably from laboratory to laboratory, institution to institution, region
to region and country to country.

15. Which one is good laboratory practice?

16. Do they practice Safety culture???

17. Thank you