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Bio Stations
Station 1
• Cholesterol + O2
• Identify A&B
B + H2O2
A
ANSWER
• A) Cholesterol oxidase
• B) 4-cholesten-3-one
Station 2
• Ahmed a 26 year old male patient that came to the primary health
center requesting for a lipid profile as his doctor asked him to do
one every year his past history is that he suffers form Angina and
takes beta blockers as treatment for his hypertension, he is also a
heavy smoker.
Results obtained during TCM in lab using spectrophotometer
• Total cholesterol=285mg/dl
• HDL=35mg/dl
• Triglycerides:250mg/dl
• (A)Calculate the NON-HDL level, interpret your patients profile.
• (B) List 2 advantages of using the non-HDL cholesterol formula.
ANSWER
• A) (NON-HDL-C) = T.C - (HDL-C)
= 285-35=250 mg/dl (HIGH) Normal <130
• INTERPRETATION:
• T.C: 285 mg/dl (HIGH >240) Normal:<200 Border line: 200-239
• HDL-C: 35 mg/dl (LOW<40) Normal:40-60 desirable: >60
• T.G: 250mg/dl (HIGH>200) Normal:<150 border line:150-199
• B) more accurate than friewald formula
• includes not only LDL-C but also other lipoprotein as IDL &VLDL
Station 3
•Mention 3 condition are invalid of
use friedwald (FF) formula
ANSWER
1. presence of chylomicrons
2. TG is greater than 400 mg/dl
3. presence of dysbetalipoprotinemia
Station 4
1. Mention Causes of low HDL-C
2. Mention normal range of HDL-C
ANSWER
1)
• ↑serum TG
• Obesity & lack exercise
• ↑ smoking
• Type 2 diabetes
• Drugs as β blockers
• Genetics factors
2) Normal: 40-60 mg/dl
Station 5
•Mention 3 things we should avoid during
preparation for lipid profile test
ANSWER
1. Avoid eat high fat foot before test
2. Avoid drink alcohol before test
3. Avoid stress / inflammation / infection / other acute
disorder → ↑ TC / TG
Station 6
1-Mention name of method used to measures serum cholesterol
2- H2O2 + 4-amino-antipyrine + phenol
• Identify A & B
A B + 4H2O2
ANSWER
1. Enzymatic Colorimetric Method
2. A) Peroxidase
B) Red quinoneimine
Bio Stations.pptx

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Bio Stations.pptx

  • 2. Station 1 • Cholesterol + O2 • Identify A&B B + H2O2 A
  • 3. ANSWER • A) Cholesterol oxidase • B) 4-cholesten-3-one
  • 4. Station 2 • Ahmed a 26 year old male patient that came to the primary health center requesting for a lipid profile as his doctor asked him to do one every year his past history is that he suffers form Angina and takes beta blockers as treatment for his hypertension, he is also a heavy smoker. Results obtained during TCM in lab using spectrophotometer • Total cholesterol=285mg/dl • HDL=35mg/dl • Triglycerides:250mg/dl • (A)Calculate the NON-HDL level, interpret your patients profile. • (B) List 2 advantages of using the non-HDL cholesterol formula.
  • 5. ANSWER • A) (NON-HDL-C) = T.C - (HDL-C) = 285-35=250 mg/dl (HIGH) Normal <130 • INTERPRETATION: • T.C: 285 mg/dl (HIGH >240) Normal:<200 Border line: 200-239 • HDL-C: 35 mg/dl (LOW<40) Normal:40-60 desirable: >60 • T.G: 250mg/dl (HIGH>200) Normal:<150 border line:150-199 • B) more accurate than friewald formula • includes not only LDL-C but also other lipoprotein as IDL &VLDL
  • 6. Station 3 •Mention 3 condition are invalid of use friedwald (FF) formula
  • 7. ANSWER 1. presence of chylomicrons 2. TG is greater than 400 mg/dl 3. presence of dysbetalipoprotinemia
  • 8. Station 4 1. Mention Causes of low HDL-C 2. Mention normal range of HDL-C
  • 9. ANSWER 1) • ↑serum TG • Obesity & lack exercise • ↑ smoking • Type 2 diabetes • Drugs as β blockers • Genetics factors 2) Normal: 40-60 mg/dl
  • 10. Station 5 •Mention 3 things we should avoid during preparation for lipid profile test
  • 11. ANSWER 1. Avoid eat high fat foot before test 2. Avoid drink alcohol before test 3. Avoid stress / inflammation / infection / other acute disorder → ↑ TC / TG
  • 12. Station 6 1-Mention name of method used to measures serum cholesterol 2- H2O2 + 4-amino-antipyrine + phenol • Identify A & B A B + 4H2O2
  • 13. ANSWER 1. Enzymatic Colorimetric Method 2. A) Peroxidase B) Red quinoneimine