This document discusses lipid profiles, which are a group of blood lipid tests done to evaluate the risk of atherosclerosis. It outlines what a lipid profile measures, including total cholesterol, triglycerides, HDL, LDL, and ratios. Normal levels for each component are provided. The document also explains how to collect and handle blood samples for lipid profiles and gives the Friedewald equation for calculating LDL from other lipid levels.

1. LIPID PROFILE
By Dr.Sharda D. Sarwade (Deshmukh )

2.  Plasma contains many lipids such as triglycerides, cholesterol
,phospholipids and minimum amount of free fatty acids.
 Most of these lipids Iare present in lipoproteins, dietary
triglycerides are incorporated in chylomicron while as
endogenious /hepatic TGs are present in VLDL.
 IN various clinical labortaries Lipid profile is carried out to
evaluate the risk of Atherosclerosis.
 Under lipid profile – serum total cholesterol, serum triglyceride
,VLDL ,HDL-C ,LDL-C these parameters are includes.
 When TGs level significantly higher predispose to pancratitis ,
that’s why Sr.TGs determination is important.

3.  Collection of sample
 Patient preparation – 10 or 12 hrs fasting.
 Specimen – Blood
 Routine venipucture ,
 Container –Light green tube
 Anticoagulant --- lithium heparin or EDTA.
 IF SERUM –plaine vial.

4. Lipid profile
 A group of blood lipids tests done to evaluate the risk of
atherosclerosis in an individual . Alterations in the levels of blood
lipids are independent risk factors for atherosclerosis .
The evaluation of cholesterol content of HDL and LDL is important
as they are involved in the cholesterol transport .
This includes the estimation of the following
 1.Total cholesterol
 2.VLDL
 3.HDL cholesterol
 4. LDL Cholesterol
 5.Triglyceride
 6.Total cholesterol : HDL –C
 7.LDL-C : HDL-C

6. NORMAL LIPID PROFILE
1.Total cholesterol --- Less than 200mg%
2.VLDL ------- up to 40 mg%
3.HDL cholesterol ------- Less than 160 mg%
4. LDL Cholesterol ------- More than 40 mg%
5.Triglyceride --------------Less than 150 mg%
6.Total cholesterol : HDL –C – Less than 5
7.LDL-C : HDL-C ------------------ Less than 3
FRIEDEWALD EQUATION
VLDL-C = TG/5
Total cholesterol (TC) = HDL-C +LDL-C +VLDL
LDL-C = TC – (HDL –C + VLDL-C )

7. Parameters Low risk Borderline risk High risk
Total cholesterol
(mg%)
less than 200 200- 240 more than 240
LDL Cholesterol
(mg%)
less 130 130 – 160 more than 160
HDL Cholesterol
(mg%)
more than 60 35 - 60 less than 35
Triglyceride(mg%) less than 150 200- 400 more than400

11. Thank you