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Biopesticide seed treatments for
improved growth of maize seedlings

                       Daniel M. Kang`ethe
 Research and Development Team, The Real IPM Company, (Kenya) Ltd


            First Bio-Innovate Regional Scientific
     Conference, Addis Ababa, Ethiopia, 25-27 February
                            2013



                                                 © The Real IPM Company
                                                   Thika, Kenya
summary

- Objective

- Background

- Methodology/procedure

- Criteria of evaluating growth promotion.

- Assessment

- Conclusion
                                     © The Real IPM Company
                                       Thika, Kenya
Back ground

Consortium of:


Jomo Kenyatta University of Agriculture and
Technology (Kenya) (JKUAT)

Alpha Seeds, Tanzania.


The Real IPM Company (K) Ltd, Kenya




                                     © The Real IPM Company
                                       Thika, Kenya
Real IPM

Biocontrol company based in Thika, Kenya


Delivery of Biopesticide on the seed.



Many advantages of seed delivery




                                        © The Real IPM Company
                                          Thika, Kenya
Research Objective



To assess the growth promoting effect of
  Biopesticides (T. asperellum, B. subtilis, M.
  anisopiliae and T. asperellum + M. anisopiliae)
  as seed treatment on maize.




                                     © The Real IPM Company
                                       Thika, Kenya
Procedure

Origin of Biopesticides

  Manufactured by Real IPM Kenya.

  Formulated specially for seed coating.


  Maize was used as test plant.


Design for on farm application

                                     © The Real IPM Company
                                       Thika, Kenya
Procedure cont…




                  © The Real IPM Company
                    Thika, Kenya
Procedure cont..




                   © The Real IPM Company
                     Thika, Kenya
Procedure cont…




Above plate pictures of (a) Seed coated maize sample and the control
and (b) colony growth
                                                  © The Real IPM Company
                                                    Thika, Kenya
Treatments

1.   T. asperellum
2.   M. anisopiliae
3.   B. subtilis
4.   T. asperellum + M. anisopiliae
5.   Control



Germination and
seedling growth assessments
                                  © The Real IPM Company
                                    Thika, Kenya
Parameters


•   Germination
•   Tap root length
•   Number of feeder roots
•   Shoot height
•   Fresh weight of the seedlings




                                    © The Real IPM Company
                                      Thika, Kenya
Results - Germination
                100
                 90                           83.07                                83.33
                        79.6                              77.33
                 80
GERMINATION %


                 70
                                   54
                 60
                 50
                 40
                 30
                 20
                 10
                  0
                      BACILLUS   CONTROL   METARHIZIUM TRICHODERMA TRICHODERMA
                                                                        +
                                                                   METARHIZIUM
                                           TREATMENTS

                                                          © The Real IPM Company
                                                            Thika, Kenya
Shoot length
                    10                                                             8.92

                     9                           8.31

                                                            7.34
                     8
SHOOT LENGTH (cm)
                           7.27


                     7
                     6
                                      4.72
                     5
                     4
                     3
                     2
                     1
                     0
                         BACILLUS   CONTROL     MET +      METARH               TRICHO
                                               TRICHO
                                              TREATMENTS

                                                                   © The Real IPM Company
                                                                     Thika, Kenya
Tap root length

                       25
                                                  21.53
TAP ROOT LENGTH (cm)                                       20.53
                              19.21

                       20
                                                                                  15.59


                       15                12.01




                       10


                        5


                        0
                            BACILLUS   CONTROL    MET +   METARH               TRICHO
                                                 TRICHO




                                                                   © The Real IPM Company
                                                                     Thika, Kenya
Number of feeder roots
                         7

                                                                    5.93
                                5.6
                         6
                                                                                            5.4
NUMBER OF FEEDER ROOTS
                                                         5.27


                         5                4.4



                         4



                         3



                         2



                         1



                         0

                             BACILLUS   CONTROL     MET + TRICHO   METARH               TRICHO
                                                  TREATMENTS


                                                                           © The Real IPM Company
                                                                             Thika, Kenya
© The Real IPM Company
  Thika, Kenya
Assessment

How many microbes on the seed?


Biopesticides            cfu`s/ Maize seed
Trichoderma asperellum   9.2 X10   7



Bacillus subtilis        7.5 X10   6



Metarhizium anisopliae   1.3 X10   6




                                       © The Real IPM Company
                                         Thika, Kenya
Conclusion

   Biopesticides enhance growth

   Act as growth promoters

   Widely reported effect

   Complex mechanism

   Other pest and disease benefits
   (See JKUAT paper)

                             © The Real IPM Company
                               Thika, Kenya
Conclusion

   What next

   Cropping trials have begun

   Seed company application
   (as opposed to on farm treatment)

   Looking for seed company
   partners in the region

   Please contact Real IPM/me
                                       © The Real IPM Company
                                         Thika, Kenya
Contacts:
info@realipm.com
www.realipm.com

   Thank you
               © The Real IPM Company
                 Thika, Kenya

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Biopesticide seed treatments for improved growth of maize seedlings

  • 1. Biopesticide seed treatments for improved growth of maize seedlings Daniel M. Kang`ethe Research and Development Team, The Real IPM Company, (Kenya) Ltd First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 February 2013 © The Real IPM Company Thika, Kenya
  • 2. summary - Objective - Background - Methodology/procedure - Criteria of evaluating growth promotion. - Assessment - Conclusion © The Real IPM Company Thika, Kenya
  • 3. Back ground Consortium of: Jomo Kenyatta University of Agriculture and Technology (Kenya) (JKUAT) Alpha Seeds, Tanzania. The Real IPM Company (K) Ltd, Kenya © The Real IPM Company Thika, Kenya
  • 4. Real IPM Biocontrol company based in Thika, Kenya Delivery of Biopesticide on the seed. Many advantages of seed delivery © The Real IPM Company Thika, Kenya
  • 5. Research Objective To assess the growth promoting effect of Biopesticides (T. asperellum, B. subtilis, M. anisopiliae and T. asperellum + M. anisopiliae) as seed treatment on maize. © The Real IPM Company Thika, Kenya
  • 6. Procedure Origin of Biopesticides Manufactured by Real IPM Kenya. Formulated specially for seed coating. Maize was used as test plant. Design for on farm application © The Real IPM Company Thika, Kenya
  • 7. Procedure cont… © The Real IPM Company Thika, Kenya
  • 8. Procedure cont.. © The Real IPM Company Thika, Kenya
  • 9. Procedure cont… Above plate pictures of (a) Seed coated maize sample and the control and (b) colony growth © The Real IPM Company Thika, Kenya
  • 10. Treatments 1. T. asperellum 2. M. anisopiliae 3. B. subtilis 4. T. asperellum + M. anisopiliae 5. Control Germination and seedling growth assessments © The Real IPM Company Thika, Kenya
  • 11. Parameters • Germination • Tap root length • Number of feeder roots • Shoot height • Fresh weight of the seedlings © The Real IPM Company Thika, Kenya
  • 12. Results - Germination 100 90 83.07 83.33 79.6 77.33 80 GERMINATION % 70 54 60 50 40 30 20 10 0 BACILLUS CONTROL METARHIZIUM TRICHODERMA TRICHODERMA + METARHIZIUM TREATMENTS © The Real IPM Company Thika, Kenya
  • 13. Shoot length 10 8.92 9 8.31 7.34 8 SHOOT LENGTH (cm) 7.27 7 6 4.72 5 4 3 2 1 0 BACILLUS CONTROL MET + METARH TRICHO TRICHO TREATMENTS © The Real IPM Company Thika, Kenya
  • 14. Tap root length 25 21.53 TAP ROOT LENGTH (cm) 20.53 19.21 20 15.59 15 12.01 10 5 0 BACILLUS CONTROL MET + METARH TRICHO TRICHO © The Real IPM Company Thika, Kenya
  • 15. Number of feeder roots 7 5.93 5.6 6 5.4 NUMBER OF FEEDER ROOTS 5.27 5 4.4 4 3 2 1 0 BACILLUS CONTROL MET + TRICHO METARH TRICHO TREATMENTS © The Real IPM Company Thika, Kenya
  • 16. © The Real IPM Company Thika, Kenya
  • 17. Assessment How many microbes on the seed? Biopesticides cfu`s/ Maize seed Trichoderma asperellum 9.2 X10 7 Bacillus subtilis 7.5 X10 6 Metarhizium anisopliae 1.3 X10 6 © The Real IPM Company Thika, Kenya
  • 18. Conclusion Biopesticides enhance growth Act as growth promoters Widely reported effect Complex mechanism Other pest and disease benefits (See JKUAT paper) © The Real IPM Company Thika, Kenya
  • 19. Conclusion What next Cropping trials have begun Seed company application (as opposed to on farm treatment) Looking for seed company partners in the region Please contact Real IPM/me © The Real IPM Company Thika, Kenya
  • 20. Contacts: info@realipm.com www.realipm.com Thank you © The Real IPM Company Thika, Kenya