Autonomous model building with a preponderance of well annotated assay protocols
•
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Combining large amounts of publicly available structure-activity data with assays that have carefully curated annotations opens the door to a number of ways to analyze the data behind the scenes. Combining fully machine readable input for a diverse variety of projects with modelling techniques that can be used without fussy parametrization allows models to be created and updated whenever new data arrives. Predictions from these models can be integrated into normal searching and visualization workflows, without any need for the user to opt-in or make extra decisions. This approach is novel and different from the way structure-activity models are normally deployed: useful predictions can be presented ubiquitously with literally zero additional work on behalf of the user. We will present our efforts to date regarding ways to both passively and actively draw attention to important drug discovery trends while exploring compounds and assays.
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1PhylogeneticAnalysisHomeworkassignmentThisa.docx
1
Phylogenetic Analysis Homework assignment
This assignment will be completed on your own and turned in the week of 11/8-11/10.
Introduction
Molecular evolution is the study of how proteins and nucleic acids evolve. Included in this
field are studies of mutations and chromosomal rearrangements, the evolutionary process,
the identification of sequence patterns conferring function in proteins and nucleic acids,
and the reconstruction of the evolutionary history of organisms and the molecules that
they make. All of these studies rely on comparisons of nucleotide or amino acid sequences.
In this tutorial, you will be introduced to some of the fundamental principles of molecular
evolution and the types of bioinformatics tools that are used in evolutionary studies. We
will begin by carrying out a manual sequence comparison, so that the basic concepts can
be introduced, and the remainder of the project will be carried out at The Biology
Workbench, a set of bioinformatics analysis programs managed by The San Diego
Supercomputing Center at the University of California, San Diego.
Objectives
• To introduce the principles of molecular evolution
• To acquaint you with the tools that are available to compare nucleotide and
amino acid sequences
• To learn about the use of protein sequences in reconstructions of evolutionary history
Project
Branching evolution occurs when one ancestral species gives rise to two or more progeny
species. However, speciation events don't involve the vast majority of the genes in a
genome. That is, for most genes, both of the progeny species inherit identical genes from
the ancestor. Following speciation, these genes evolve independently in the separate
lineages. Studies of molecular evolution therefore rely heavily on comparisons of related
sequences from different organisms.
Shown below is an alignment of two homologous sequences that we will use as a starting
place. Homologous sequences are sequences that have descended from a common
ancestral sequence. You can't meaningfully compare sequences unless they are
homologous. This alignment uses the single letter amino acid code, in which G represents
glycine, Q represents glutamine, etc. The aligned proteins have been shown to be involved
in the metabolism of similar, but different, toxic compounds. As you can see, these amino
acid sequences are very similar and it is easy to recognize that they are related by common
descent.
2
dntAc: KMGVDDEVIVSRQNDGSVR
nahAc: KMGIDDEVIVSRQSDGSIR
An expanded version of this alignment is shown below. In this expanded alignment, both
the amino acids and the corresponding DNA nucleotides are shown. For ease of analysis,
the codons have been broken into separate entries in a table.
Alignment of nahAc and dntAc sequences.
K M G V D E V I V
dntAc AAA ATG GGC GTC GAT GAA GTC ATC GTC
nahAc ...
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2) Reducing volumes in library preparation steps to decrease usage of costly reagents while maintaining performance.
3) Recommending sequencing strategies like read lengths that maximize the proportion of read pairs containing junction adaptors, important for scaffolding.
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1PhylogeneticAnalysisHomeworkassignmentThisa.docx
1
Phylogenetic Analysis Homework assignment
This assignment will be completed on your own and turned in the week of 11/8-11/10.
Introduction
Molecular evolution is the study of how proteins and nucleic acids evolve. Included in this
field are studies of mutations and chromosomal rearrangements, the evolutionary process,
the identification of sequence patterns conferring function in proteins and nucleic acids,
and the reconstruction of the evolutionary history of organisms and the molecules that
they make. All of these studies rely on comparisons of nucleotide or amino acid sequences.
In this tutorial, you will be introduced to some of the fundamental principles of molecular
evolution and the types of bioinformatics tools that are used in evolutionary studies. We
will begin by carrying out a manual sequence comparison, so that the basic concepts can
be introduced, and the remainder of the project will be carried out at The Biology
Workbench, a set of bioinformatics analysis programs managed by The San Diego
Supercomputing Center at the University of California, San Diego.
Objectives
• To introduce the principles of molecular evolution
• To acquaint you with the tools that are available to compare nucleotide and
amino acid sequences
• To learn about the use of protein sequences in reconstructions of evolutionary history
Project
Branching evolution occurs when one ancestral species gives rise to two or more progeny
species. However, speciation events don't involve the vast majority of the genes in a
genome. That is, for most genes, both of the progeny species inherit identical genes from
the ancestor. Following speciation, these genes evolve independently in the separate
lineages. Studies of molecular evolution therefore rely heavily on comparisons of related
sequences from different organisms.
Shown below is an alignment of two homologous sequences that we will use as a starting
place. Homologous sequences are sequences that have descended from a common
ancestral sequence. You can't meaningfully compare sequences unless they are
homologous. This alignment uses the single letter amino acid code, in which G represents
glycine, Q represents glutamine, etc. The aligned proteins have been shown to be involved
in the metabolism of similar, but different, toxic compounds. As you can see, these amino
acid sequences are very similar and it is easy to recognize that they are related by common
descent.
2
dntAc: KMGVDDEVIVSRQNDGSVR
nahAc: KMGIDDEVIVSRQSDGSIR
An expanded version of this alignment is shown below. In this expanded alignment, both
the amino acids and the corresponding DNA nucleotides are shown. For ease of analysis,
the codons have been broken into separate entries in a table.
Alignment of nahAc and dntAc sequences.
K M G V D E V I V
dntAc AAA ATG GGC GTC GAT GAA GTC ATC GTC
nahAc ...
Dario Lijtmaer - PCR Amplification
This document summarizes a short course on DNA barcoding methods that will cover equipment needed for PCR amplification, PCR protocols, product verification using agarose gels, minimizing contamination risks, and shipping and storing DNA extracts. The course will provide an overview of typical PCR protocols and note that protocols depend on sample quality and scale of operations. Small PCR volumes and use of trehalose are discussed as cost-saving measures. Primer selection is also highlighted as a key factor in success.
Similar to Autonomous model building with a preponderance of well annotated assay protocols (20)
Discovery PBPK: Efficiently using machine learning & PBPK modeling to drive l...
Discovery PBPK: Efficiently using machine learning & PBPK modeling to drive l...
Large scale classification of chemical reactions from patent data
Large scale classification of chemical reactions from patent data
Using open bioactivity data for developing machine-learning prediction models...
Using open bioactivity data for developing machine-learning prediction models...
XabTracker & SeqAgent: Integrated LIMS & Sequence Analysis Tools for Antibody...
XabTracker & SeqAgent: Integrated LIMS & Sequence Analysis Tools for Antibody...
Informatics In The Manchester Centre For Integrative Systems Biology
Informatics In The Manchester Centre For Integrative Systems Biology
Mining Big datasets to create and validate machine learning models
Mining Big datasets to create and validate machine learning models
More from Alex Clark
Mixtures QSAR: modelling collections of chemicals
This document discusses representing and modeling chemical mixtures. It proposes a new data format called Mixfile or MInChI to hierarchically define mixtures and their components, including concentrations. This format aims to support cheminformatics applications like property prediction. Examples are given modeling theophylline solubility and gas absorption using mixture data. The document also describes applying similar methods to model polymer entropy of mixing using a spreadsheet dataset converted to the mixtures format. It concludes that defining mixtures in digital formats will enable greater analysis, modeling and use of mixture data.
Mixtures InChI: a story of how standards drive upstream products
This document discusses the development of Mixtures InChI (MInChI), a standard for representing chemical mixtures in a machine-readable format. MInChI was developed to address the lack of standards for mixture informatics and interoperability. The document outlines the development of open source tools to generate and edit MInChI notation, as well as efforts to build a community and integrate MInChI into commercial products and databases to enable widespread use and generation of mixture data. Future work discussed includes finalizing the MInChI specification, extending it to additional chemical entities, developing associated properties and metadata, and implementing MInChI at large scale.
Mixtures as first class citizens in the realm of informatics
Presented at Cambridge (UK) cheminformatics meeting, February 2021. Mixtures of chemicals are underutilised from an informatics point of view, and this presentation shows some of the work done by Collaborative Drug Discovery, IUPAC and InChI Trust to remedy this.
See recording: https://www.youtube.com/watch?v=0ILc0owuEzQ&list=PLfj_gc4RCduuwv9p8lh2xS1EhQ3p_Nd9S&index=1 ... my part starts at 1:05:00
Mixtures: informatics for formulations and consumer products
The document proposes standards for representing mixtures in a machine-readable format. It introduces Mixfile and MInChI (Mixtures InChI) as hierarchical and concise formats for describing mixtures. Examples of formulations are provided to demonstrate how components, concentrations, and metadata can be encoded. Potential applications of the standards are discussed, such as enabling sophisticated searches of mixture data from publications and vendors to facilitate properties prediction and hazards assessment. Adoption of the standards could help ensure the longevity and sharing of mixture data.
Coordination InChI (2019)
Preliminary survey of inorganic compounds
For extensive details and updates, see https://github.com/aclarkxyz/data_coordinchi
Chemical mixtures: File format, open source tools, example data, and mixtures...
This document discusses representing chemical mixtures using an open format called Mixfile. It proposes Mixfile as a standard format for mixtures, analogous to Molfile for individual molecules. Tools were created to edit and manipulate Mixfiles. Over 5,600 real-world mixture examples were extracted from text and represented in the Mixfile format. A MInChI notation was also defined as a condensed representation of mixtures. Future work is proposed to integrate mixture definitions and lookups into electronic lab notebooks and improve automated extraction of mixture information from text.
ACS CINF Luncheon talk (Boston 2018)
Leveling up chemical information for the era of big data: an appeal to make data a top priority in the peer review process...
Representing molecules with minimalism: A solution to the entropy of informatics
Cheminformatics as we know it is possible because so many molecular structures can be represented with datastructures and rules that are at first glance quite trivial. This first impression is highly misleading, since even within supposedly well behaved domains, edge cases arising from issues such as resonance, tautomerization, symmetry and stereochemistry - to name but a few - quickly add up. To supplement these genuine challenges, there is a whole additional class of problems caused by the mismatch between chemists' understanding of molecules and the datatypes that are necessary to capture a structure for informatics purposes. This line is blurred by the convenience of representing structures in a form that is very closely related to the diagram styles that have been in use since the dawn of chemistry. There are currently four major approaches to structure representation: connection tables (e.g. MDL Molfile), sketches (e.g. ChemDraw), canonical strings (e.g. SMILES and InChI) and atomic models (numerous 3D formats). Not only do all of these approaches have valid use cases, but they are deceptively incompatible with each other, even when addressing identical needs. Almost without exception, format conversions are not commutative, and every translation involves losing some amount of data. Given that recording chemical structures in machine readable form has become such a critical part of scientific research, it is essential to define a fundamental representation that captures the key structural definition asserted by the experimental chemist, for a broad and useful range of molecules, and ideally in a way that is closely related to visual drawing mnemonics. The number of data concepts needed to satisfy these conditions is quite small, and is mostly satisfied by the most commonly used subset of the venerable MDL Molfile format. This presentation will discuss how this subset, with a few minor corrections and clarifications, can and should be used as the reference standard for molecules, and how the informatics community can benefit from having well defined standards.
SLAS2016: Why have one model when you could have thousands?
Society for Laboratory Automation & Screening, San Diego, January 2016. Presented by Dr. Alex M. Clark. Describes the use of open data resources (ChEMBL) to build target-activity models for drug discovery and toxicity prediction, on a massive scale, using a fully automated process. Concludes with a demo of the PolyPharma app, which shows how these models can be used for prospective drug discovery.
The anatomy of a chemical reaction: Dissection by machine learning algorithms
This document discusses using machine learning algorithms to analyze chemical reaction data. It describes how current reaction reporting formats are not well-suited for computational analysis. A more structured reporting format is proposed to fully describe reactions in a digitally friendly way, including specifying reactants, products, quantities, yields, and metrics like atom efficiency. This structured data would allow modeling of reaction substitutability and enable large-scale machine learning of chemical transformations.
Compact models for compact devices: Visualisation of SAR using mobile apps
Presented at American Chemical Society meeting, Boston, 2015. Describes how cheminformatics algorithms and visualisation interfaces have advanced on mobile apps to cover a diverse variety of functionality, increasingly calculated on the device itself rather than deferring to a web service. Culminates in a demo of the PolyPharma app prototype (see http://cheminf20.org/2015/08/06/the-polypharma-app-a-mash-up-of-ideas-and-technology)
Green chemistry in chemical reactions: informatics by design
Chemical informatics technology can be of assistance to chemists for describing reactions in numerous ways, including calculating green chemistry metrics such as process mass intensity, E-factor and atom economy. To facilitate this, chemical reactions have to be described in more precise detail than is the norm for most chemists. There are also numerous practical ways to add more green chemistry functionality to lab notebooks, such as enumerating searchable reaction transforms for environmentally favourable reactions, automatically looking up toxicity and hazard information, and others which are mentioned in the slides.
This presentation was given at the Green Chemistry & Engineering conference in 2015 (Americal Chemical Society Green Chemistry Insititute).
ICCE 2014: The Green Lab Notebook
Green chemistry is an important subject that needs to be a part of every chemist's education, as well as a part of the daily routine of the professional synthetic chemist. This talk describes how a new app can be used to bring green chemistry metrics to reaction descriptions, once they are captured in a proper cheminformatics format. It also describes some of the additional data resources that can be incorporated into the user experience, and how this helps both students and professionals.
Cloud hosted APIs for cheminformatics on mobile devices (ACS Dallas 2014)
Mobile apps for cheminformatics are quite powerful on their own, but can be significantly boosted by connecting them with cloud-hosted functionality. This talk explores the range of functionality that can be covered simply by making use of apps with stateless webservices, i.e. anonymous access without persistent data.
Building a mobile reaction lab notebook (ACS Dallas 2014)
This document discusses building a mobile electronic lab notebook focused on chemical reactions called the Green Lab Notebook. It would allow users to draw chemical structures, balance reactions, and calculate quantities, yields, and green metrics. Key features include digitally capturing reaction data, prioritizing computer-friendly data structures and intuitive workflows, and linking to external databases for solvent data, sustainable feedstocks, and curated green reaction transforms. The goal is to facilitate recording, analyzing, and promoting the reuse of experimental reaction data in a sustainable chemistry context.
Reaction Lab Notebooks for Mobile Devices - Alex M. Clark - GDCh 2013
Presented at 2013 the German Chem[o]informatics Conference in Fulda, 2013: entitled "Putting together the pieces: building a reaction-centric electronic lab notebook for mobile devices".
Alex Clark : NETTAB 2013
Cheminformatics
workflows using the
mobile + cloud platform. Presentation by Dr. Alex M. Clark of Molecular Materials Informatics at the NETTAB 2013 meeting in Venice, Italy. The presentation introduces the significance of mobile apps in science, and the scope of their capabilities in chemical structure informatics. The bulk of the talk describes an account of a preliminary workflow using open science data to search for viable leads for a cure for tuberculosis. The workflow described makes use of a combination of mobile, cloud and conventional desktop-based technology, all stitched together by facile communication, sharing and collaboration features.
Open Drug Discovery Teams @ Hacking Health Montreal
Alex Clark of Molecular Materials Informatics (http://molmatinf.com) presents the Open Drug Discovery Teams project to the Hacking Health Montreal audience, April 2013.
Pistoia Alliance App Strategy
Presentation to the American Chemical Society (New Orleans, April 2013) describing the app strategy of the Pistoia Alliance.
Mobile+Cloud: a viable replacement for desktop cheminformatics?
Introduces the prospect of performing cheminformatics workflows using mobile devices for the UI and cloud resources for heavy duty computation. Describes a sample workflow using the SAR Table app to propose potential new drugs for eliminating malaria.
More from Alex Clark (20)
Mixtures InChI: a story of how standards drive upstream products
Mixtures InChI: a story of how standards drive upstream products
Mixtures as first class citizens in the realm of informatics
Mixtures as first class citizens in the realm of informatics
Mixtures: informatics for formulations and consumer products
Mixtures: informatics for formulations and consumer products
Chemical mixtures: File format, open source tools, example data, and mixtures...
Chemical mixtures: File format, open source tools, example data, and mixtures...
Representing molecules with minimalism: A solution to the entropy of informatics
Representing molecules with minimalism: A solution to the entropy of informatics
SLAS2016: Why have one model when you could have thousands?
SLAS2016: Why have one model when you could have thousands?
The anatomy of a chemical reaction: Dissection by machine learning algorithms
The anatomy of a chemical reaction: Dissection by machine learning algorithms
Compact models for compact devices: Visualisation of SAR using mobile apps
Compact models for compact devices: Visualisation of SAR using mobile apps
Green chemistry in chemical reactions: informatics by design
Green chemistry in chemical reactions: informatics by design
Cloud hosted APIs for cheminformatics on mobile devices (ACS Dallas 2014)
Cloud hosted APIs for cheminformatics on mobile devices (ACS Dallas 2014)
Building a mobile reaction lab notebook (ACS Dallas 2014)
Building a mobile reaction lab notebook (ACS Dallas 2014)
Reaction Lab Notebooks for Mobile Devices - Alex M. Clark - GDCh 2013
Reaction Lab Notebooks for Mobile Devices - Alex M. Clark - GDCh 2013
Open Drug Discovery Teams @ Hacking Health Montreal
Open Drug Discovery Teams @ Hacking Health Montreal
Mobile+Cloud: a viable replacement for desktop cheminformatics?
Mobile+Cloud: a viable replacement for desktop cheminformatics?
Recently uploaded
在线办理(salfor毕业证书)索尔福德大学毕业证毕业完成信一模一样
学校原件一模一样【微信：741003700 】《(salfor毕业证书)索尔福德大学毕业证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Gadgets for management of stored product pests_Dr.UPR.pdf
Insectsplayamajorroleinthedeteriorationoffoodgrainscausingbothquantitativeandqualitativelosses
Wellprovedthatnogranariescanbefilledwithgrainswithoutinsectsastheharvestedproducecontainegg(or)larvae(or)pupae(or)adultinsectinthembecauseoffieldcarryoverinfestationwhichcannotbeavoidedindevelopingcountrieslikeIndia
Simpletechnologiesfortimelydetectionofinsectsinthestoredproduceandtherebyplantimelycontrolmeasures
Applied Science: Thermodynamics, Laws & Methodology.pdf
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
Travis Hills of MN is Making Clean Water Accessible to All Through High Flux ...
By harnessing the power of High Flux Vacuum Membrane Distillation, Travis Hills from MN envisions a future where clean and safe drinking water is accessible to all, regardless of geographical location or economic status.
Immersive Learning That Works: Research Grounding and Paths Forward
We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Sexuality - Issues, Attitude and Behaviour - Applied Social Psychology - Psyc...
A proprietary approach developed by bringing together the best of learning theories from Psychology, design principles from the world of visualization, and pedagogical methods from over a decade of training experience, that enables you to: Learn better, faster!
The debris of the ‘last major merger’ is dynamically young
The Milky Way’s (MW) inner stellar halo contains an [Fe/H]-rich component with highly eccentric orbits, often referred to as the
‘last major merger.’ Hypotheses for the origin of this component include Gaia-Sausage/Enceladus (GSE), where the progenitor
collided with the MW proto-disc 8–11 Gyr ago, and the Virgo Radial Merger (VRM), where the progenitor collided with the
MW disc within the last 3 Gyr. These two scenarios make different predictions about observable structure in local phase space,
because the morphology of debris depends on how long it has had to phase mix. The recently identified phase-space folds in Gaia
DR3 have positive caustic velocities, making them fundamentally different than the phase-mixed chevrons found in simulations
at late times. Roughly 20 per cent of the stars in the prograde local stellar halo are associated with the observed caustics. Based
on a simple phase-mixing model, the observed number of caustics are consistent with a merger that occurred 1–2 Gyr ago.
We also compare the observed phase-space distribution to FIRE-2 Latte simulations of GSE-like mergers, using a quantitative
measurement of phase mixing (2D causticality). The observed local phase-space distribution best matches the simulated data
1–2 Gyr after collision, and certainly not later than 3 Gyr. This is further evidence that the progenitor of the ‘last major merger’
did not collide with the MW proto-disc at early times, as is thought for the GSE, but instead collided with the MW disc within
the last few Gyr, consistent with the body of work surrounding the VRM.
The binding of cosmological structures by massless topological defects
Assuming spherical symmetry and weak field, it is shown that if one solves the Poisson equation or the Einstein field
equations sourced by a topological defect, i.e. a singularity of a very specific form, the result is a localized gravitational
field capable of driving flat rotation (i.e. Keplerian circular orbits at a constant speed for all radii) of test masses on a thin
spherical shell without any underlying mass. Moreover, a large-scale structure which exploits this solution by assembling
concentrically a number of such topological defects can establish a flat stellar or galactic rotation curve, and can also deflect
light in the same manner as an equipotential (isothermal) sphere. Thus, the need for dark matter or modified gravity theory is
mitigated, at least in part.
The cost of acquiring information by natural selection
This is a short talk that I gave at the Banff International Research Station workshop on Modeling and Theory in Population Biology. The idea is to try to understand how the burden of natural selection relates to the amount of information that selection puts into the genome.
It's based on the first part of this research paper:
The cost of information acquisition by natural selection
Ryan Seamus McGee, Olivia Kosterlitz, Artem Kaznatcheev, Benjamin Kerr, Carl T. Bergstrom
bioRxiv 2022.07.02.498577; doi: https://doi.org/10.1101/2022.07.02.498577
Compexometric titration/Chelatorphy titration/chelating titration
Classification
Metal ion ion indicators
Masking and demasking reagents
Estimation of Magnisium sulphate
Calcium gluconate
Complexometric Titration/ chelatometry titration/chelating titration, introduction, Types-
1.Direct Titration
2.Back Titration
3.Replacement Titration
4.Indirect Titration
Masking agent, Demasking agents
formation of complex
comparition between masking and demasking agents,
Indicators/Metal ion indicators/ Metallochromic indicators/pM indicators,
Visual Technique,PM indicators (metallochromic), Indicators of pH, Redox Indicators
Instrumental Techniques-Photometry
Potentiometry
Miscellaneous methods.
Complex titration with EDTA.
快速办理(UAM毕业证书)马德里自治大学毕业证学位证一模一样
学校原件一模一样【微信：741003700 】《(UAM毕业证书)马德里自治大学毕业证学位证》【微信：741003700 】学位证，留信认证（真实可查，永久存档）原件一模一样纸张工艺/offer、雅思、外壳等材料/诚信可靠,可直接看成品样本，帮您解决无法毕业带来的各种难题！外壳，原版制作，诚信可靠，可直接看成品样本。行业标杆！精益求精，诚心合作，真诚制作！多年品质 ,按需精细制作，24小时接单,全套进口原装设备。十五年致力于帮助留学生解决难题，包您满意。
本公司拥有海外各大学样板无数，能完美还原。
1:1完美还原海外各大学毕业材料上的工艺：水印，阴影底纹，钢印LOGO烫金烫银，LOGO烫金烫银复合重叠。文字图案浮雕、激光镭射、紫外荧光、温感、复印防伪等防伪工艺。材料咨询办理、认证咨询办理请加学历顾问Q/微741003700
【主营项目】
一.毕业证【q微741003700】成绩单、使馆认证、教育部认证、雅思托福成绩单、学生卡等！
二.真实使馆公证(即留学回国人员证明,不成功不收费)
三.真实教育部学历学位认证（教育部存档！教育部留服网站永久可查）
四.办理各国各大学文凭(一对一专业服务,可全程监控跟踪进度)
如果您处于以下几种情况：
◇在校期间，因各种原因未能顺利毕业……拿不到官方毕业证【q/微741003700】
◇面对父母的压力，希望尽快拿到；
◇不清楚认证流程以及材料该如何准备；
◇回国时间很长，忘记办理；
◇回国马上就要找工作，办给用人单位看；
◇企事业单位必须要求办理的
◇需要报考公务员、购买免税车、落转户口
◇申请留学生创业基金
留信网认证的作用:
1:该专业认证可证明留学生真实身份
2:同时对留学生所学专业登记给予评定
3:国家专业人才认证中心颁发入库证书
4:这个认证书并且可以归档倒地方
5:凡事获得留信网入网的信息将会逐步更新到个人身份内，将在公安局网内查询个人身份证信息后，同步读取人才网入库信息
6:个人职称评审加20分
7:个人信誉贷款加10分
8:在国家人才网主办的国家网络招聘大会中纳入资料，供国家高端企业选择人才
Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
Current descriptions of immersive learning cases are often difficult or impossible to compare. This is due to a myriad of different options on what details to include, which aspects are relevant, and on the descriptive approaches employed. Also, these aspects often combine very specific details with more general guidelines or indicate intents and rationales without clarifying their implementation. In this paper we provide a method to describe immersive learning cases that is structured to enable comparisons, yet flexible enough to allow researchers and practitioners to decide which aspects to include. This method leverages a taxonomy that classifies educational aspects at three levels (uses, practices, and strategies) and then utilizes two frameworks, the Immersive Learning Brain and the Immersion Cube, to enable a structured description and interpretation of immersive learning cases. The method is then demonstrated on a published immersive learning case on training for wind turbine maintenance using virtual reality. Applying the method results in a structured artifact, the Immersive Learning Case Sheet, that tags the case with its proximal uses, practices, and strategies, and refines the free text case description to ensure that matching details are included. This contribution is thus a case description method in support of future comparative research of immersive learning cases. We then discuss how the resulting description and interpretation can be leveraged to change immersion learning cases, by enriching them (considering low-effort changes or additions) or innovating (exploring more challenging avenues of transformation). The method holds significant promise to support better-grounded research in immersive learning.
(June 12, 2024) Webinar: Development of PET theranostics targeting the molecu...
(June 12, 2024) Webinar: Development of PET theranostics targeting the molecu...Scintica Instrumentation
Targeting Hsp90 and its pathogen Orthologs with Tethered Inhibitors as a Diagnostic and Therapeutic Strategy for cancer and infectious diseases with Dr. Timothy Haystead.11.1 Role of physical biological in deterioration of grains.pdf
Storagedeteriorationisanyformoflossinquantityandqualityofbio-materials.
Themajorcausesofdeteriorationinstorage
•Physical
•Biological
•Mechanical
•Chemical
Storageonlypreservesquality.Itneverimprovesquality.
Itisadvisabletostartstoragewithqualityfoodproduct.Productwithinitialpoorqualityquicklydepreciates
Recently uploaded (20)
Gadgets for management of stored product pests_Dr.UPR.pdf
Gadgets for management of stored product pests_Dr.UPR.pdf
Applied Science: Thermodynamics, Laws & Methodology.pdf
Applied Science: Thermodynamics, Laws & Methodology.pdf
Travis Hills of MN is Making Clean Water Accessible to All Through High Flux ...
Travis Hills of MN is Making Clean Water Accessible to All Through High Flux ...
Immersive Learning That Works: Research Grounding and Paths Forward
Immersive Learning That Works: Research Grounding and Paths Forward
Sexuality - Issues, Attitude and Behaviour - Applied Social Psychology - Psyc...
Sexuality - Issues, Attitude and Behaviour - Applied Social Psychology - Psyc...
The debris of the ‘last major merger’ is dynamically young
The debris of the ‘last major merger’ is dynamically young
The binding of cosmological structures by massless topological defects
The binding of cosmological structures by massless topological defects
The cost of acquiring information by natural selection
The cost of acquiring information by natural selection
Compexometric titration/Chelatorphy titration/chelating titration
Compexometric titration/Chelatorphy titration/chelating titration
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Describing and Interpreting an Immersive Learning Case with the Immersion Cub...
(June 12, 2024) Webinar: Development of PET theranostics targeting the molecu...
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aziz sancar nobel prize winner: from mardin to nobel
aziz sancar nobel prize winner: from mardin to nobel
11.1 Role of physical biological in deterioration of grains.pdf
11.1 Role of physical biological in deterioration of grains.pdf
Autonomous model building with a preponderance of well annotated assay protocols
- 1. Autonomous model building with a preponderance of well annotated assay protocols Alex M. Clark http://www.bioassayexpress.com ⚡ COLLABORATIVE DRUG DISCOVERY
- 2. Background ◈ Assay protocols are text ◈ Institutions suffering from data overload: ▷ much interest in using consistent markup ▷ semantic web ontologies: BAO, DTO, CLO, GO... ◈ Since last talk: many refinements, some new structure-activity analysis features 2 Cell-Free Homogeneous Primary HTS to Identify Inhibitors of GSK3beta Activity (1) Dispense 1 uL/well of CABPE, 0.5 uL of ATP, and 1 uL of positive control GW8510 or AB in respective wells according to plate design to 1536-well assay ready plates (Aurora 29847) that contain 2.5 nL/well of 10 mM compound using BioRAPTR (Beckman) to start the reaction. Incubate at room temperature for 60 minutes. (2) Add 2.5 uL/well of ADP-glo (Promega, V9103) with BioRAPTR, incubate at room temperature for 40 minutes (3) Add 5 uL/well of ADP-glo (Promega, V9103) with Combi nL (Thermo), incubate at room temperature for 30 minutes
- 3. Curation ◈ Web UI ◈ Machine learning ◈ Common Assay Template ◈ PubChem data 3
- 4. Public Data ◈ Designed Common Assay Template for summary ◈ Data from PubChem: ▷ 3500 assays selected ▷ mostly MLPCN ◈ Curated as a validation set ◈ PubChem also has molecules... 4
- 5. Analysis ◈ Selected: ▷ Secondary assays ▷ Infectious diseases ▷ IC50 results 5
- 6. Analysis ◈ Selected: ▷ Secondary assays ▷ Infectious diseases ▷ IC50 results 5
- 7. Molecules ◈ Per assay: convenient way to view & download... 6
- 20. Grids ◈ View known & calculated measurements, plotting assays vs. compounds... 11
- 21. Grids ◈ View known & calculated measurements, plotting assays vs. compounds... 11
- 22. Real Data Only 12 ◈ Assays are singletons by default, can be grouped...
- 25. Filling in Blanks 13 ◈ Bayesian model created for each column ◈ SAR for all assays used ◈ Missing cells replaced with predictions ◈ Calculations realtime: ▷ model on server ▷ predictions on client
- 26. Trend Detection 14 ◈ Model compound found using the Selectivity option:
- 27. Trend Detection 14 ◈ Model compound found using the Selectivity option: multidrug resistant tuberculosis
- 28. Trend Detection 14 ◈ Model compound found using the Selectivity option: multidrug resistant tuberculosis trypanosomiasis (ROC 0.56)
- 31. Future Work ◈ Current focus is UI/UX and proof-of-concept... ▷ intensive data mining is next: annotations + SAR ▷ data is ready to reveal some amazing discoveries ◈ Crowd curation may be necessary, and more detailed templates ◈ More modelling techniques, far beyond Bayesians: parallel project is underway... 16
- 32. Deployment ◈ Public ▷ http://www.bioassayexpress.com ▷ http://beta.bioassayexpress.com (curation) ◈ Vault ▷ sync with CDD Vault through API ◈ Private ▷ custom integration, installed behind firewall 17
- 33. Acknowledgments ◈ Collaborative Drug Discovery ⇨ Barry Bunin ⇨ Janice Kranz ⇨ Hande Küçük ⇨ Peter Gedeck 18 ◈ And we're hiring ⇨ resumes to: ◈ More information http://github.com/cdd/bioassay-template http://www.bioassayexpress.com http://collaborativedrug.com alex@collaborativedrug.com