The document discusses sterilization using an autoclave. It describes how an autoclave uses high pressure steam to sterilize materials. It explains the main components of an autoclave including the heating elements, temperature controller, pressure sensor, and chamber. It outlines several sterilization programs that can be selected and provides cleaning and maintenance procedures for the autoclave.
Autoclave, types of autoclave, horizontal autoclave, vertical autoclave, vacuum type autoclave, pressure cooker type autoclave. their purpose, precaution, etc....
Sterilization (or sterilisation) referring to any process that eliminates (removes) or kills (deactivates) all forms of life and other biological agents (such as prions, as well as viruses which some do not consider to be alive but are biological pathogens nonetheless), including transmissible agents (such as fungi, bacteria, viruses, prions, spore forms, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media
Autoclave, types of autoclave, horizontal autoclave, vertical autoclave, vacuum type autoclave, pressure cooker type autoclave. their purpose, precaution, etc....
Sterilization (or sterilisation) referring to any process that eliminates (removes) or kills (deactivates) all forms of life and other biological agents (such as prions, as well as viruses which some do not consider to be alive but are biological pathogens nonetheless), including transmissible agents (such as fungi, bacteria, viruses, prions, spore forms, unicellular eukaryotic organisms such as Plasmodium, etc.) present in a specified region, such as a surface, a volume of fluid, medication, or in a compound such as biological culture media
it is related with medical laboratory instrumentation and explains in very good way that what is hot air oven and its principle, working and all about it
STERILISATION, PHYSICAL METHODS OF STERILISATION, METHODS OF STERILISATION, VARIOUS METHODS OF STERILISATION, AUTOCLAVES, HOT AIR OVEN, DRY HEAT STERILISATION, MOIST HEAT STERILISATION
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes principle and working of hot air oven and autoclave.
The sterilization of surgical instruments is a process that removes all microorganisms from medical instruments before a surgery can take place. Proper sterilization ensures that all equipment has been thoroughly cleaned, sanitized and sterilized, and minimizes the risk of preventable surgical site infections. This process should be completed by a certified central sterilization technician.
The use of a machine designed to follow repeatedly and automatically a predetermined sequence of individual operations.
AUTOMATED WASHING
AUTOMATED MEDIA PREPARATORS
AUTOMATED COLLECTION AND
PROCESSING OF SAMPLES
CYTOSPIN
AUTOMATED GRAM STAINING
AUTOMATED STREAKING
SPIRAL PLATER
AUTOMATED ANTIBIOTIC -
SENSITIVITY SYSTEM
AUTOMATIC COLONY COUNTER
AUTOMATED URINE MICROSCOPY -
ANALYSER
Gator uses The UNIFLEX Brand when applying the fluid applications on roof tops. This is perfect for Commercial Building, Retail Chains, Warehouses and Storage Companies. By saving 40% off Utility bills each month and rebates from energy companies, the typical ROI is 3-4 years.
it is related with medical laboratory instrumentation and explains in very good way that what is hot air oven and its principle, working and all about it
STERILISATION, PHYSICAL METHODS OF STERILISATION, METHODS OF STERILISATION, VARIOUS METHODS OF STERILISATION, AUTOCLAVES, HOT AIR OVEN, DRY HEAT STERILISATION, MOIST HEAT STERILISATION
The above PPT includes different methods of sterilization- Dry heat, Moist heat, Radiation and Chemical methods. It also includes principle and working of hot air oven and autoclave.
The sterilization of surgical instruments is a process that removes all microorganisms from medical instruments before a surgery can take place. Proper sterilization ensures that all equipment has been thoroughly cleaned, sanitized and sterilized, and minimizes the risk of preventable surgical site infections. This process should be completed by a certified central sterilization technician.
The use of a machine designed to follow repeatedly and automatically a predetermined sequence of individual operations.
AUTOMATED WASHING
AUTOMATED MEDIA PREPARATORS
AUTOMATED COLLECTION AND
PROCESSING OF SAMPLES
CYTOSPIN
AUTOMATED GRAM STAINING
AUTOMATED STREAKING
SPIRAL PLATER
AUTOMATED ANTIBIOTIC -
SENSITIVITY SYSTEM
AUTOMATIC COLONY COUNTER
AUTOMATED URINE MICROSCOPY -
ANALYSER
Gator uses The UNIFLEX Brand when applying the fluid applications on roof tops. This is perfect for Commercial Building, Retail Chains, Warehouses and Storage Companies. By saving 40% off Utility bills each month and rebates from energy companies, the typical ROI is 3-4 years.
There have many methods of sterilization.,there have also sterilization with aseptic techniques for help to education. Easy to learn about of sterilization, and how to sterile easy to learn way. So i hope you'll enjoy and learn easily and to read about this slide.
This chapter contains sterilization techniques : physical - heat , filtration and radiations and chemical methods of sterilization in simple concise way so that mode of action is understood .
Sterilization.pdf Sterilization is the complete destruction of microorganisms...Talal Albudayri
Introduction
Sterilization is the complete destruction of
microorganisms including bacterial spores. This level
of decontamination is required for all reusable
invasive medical devices (RMD).
Ideally, sterilization methods:
• Have rapid throughput
• Are easily validated
• Are capable of processing wrapped items to
enable storage after processing without the risk of
environmental contamination of processed items.
Welcome to our Slideshare presentation on the Qualification of Autoclave, an essential process to ensure the effective sterilization of medical instruments and equipment. In this presentation, we will explore the significance of autoclave qualification, its various stages, and the critical factors involved in maintaining sterilization safety.
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for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
Safalta Digital marketing institute in Noida, provide complete applications that encompass a huge range of virtual advertising and marketing additives, which includes search engine optimization, virtual communication advertising, pay-per-click on marketing, content material advertising, internet analytics, and greater. These university courses are designed for students who possess a comprehensive understanding of virtual marketing strategies and attributes.Safalta Digital Marketing Institute in Noida is a first choice for young individuals or students who are looking to start their careers in the field of digital advertising. The institute gives specialized courses designed and certification.
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The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
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Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
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Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
1.4 modern child centered education - mahatma gandhi-2.pptx
Autoclave
1. Sterilization
Sterilization is a term referring to any process that
removes or kills all forms of microbial organisms such
as fungi, bacteria, viruses, spore forms, etc. present on a
surface, contained in a fluid, or in a compound such as
biological culture media.
Sterilization can be achieved
by applying heat, chemicals,
irradiation, high pressure,
and filtration or combinations
of them.
2. The autoclave is a
equipment used to
remove microorganisms
(Virus, Bacteria, fungus
etc.) and spores using
high pressure and high
temperature steam
sterilization.
3. PURPOSE OF THE AUTOCLAVE
1. To prepare materials for bacteriological cell
cultures(test tubes, pipettes, Petri dishes, etc.) without
contamination.
2. Prepare elements used for taking samples. (needles,
tubes, containers).
3. Sterilize contaminated material.
4. OPERATION PRINCIPLES
when the pressure higher than atmosphere a higher
temperature than 1000C needs to boil the water.
This steam contain higher energy than usual.
Autoclaves make pressurized saturated steam in a
chamber passing electrical current through a heating
element in controlled manner.
This energized steam take place required sterilization.
This method is known as steam sterilization.
5. MAIN COMPONENTS
1. Heating Elements
2. Temperature Controller
3. Pressure Sensor
4. Chamber
5. Door gasket
6. Solenoid valve
7. Water level Sensor
16. Program selection
Program nº
Mode
Temperature /
pressure
Sterilization
time
Drying time
1
Solid
121ºC / 1bar
20
-
2
Solid
126ºC / 1.33 bar
30
-
3
Solid
134C / 2 bar
18
-
4
Solid
134ºC / 2 bar
10
-
5
Liquid
121ºC / 1bar
30
-
6
Solid + Dry
121ºC / 1bar
30
35
7
Solid + Dry
126ºC / 1.33 bar
20
35
8
Solid + Dry
126ºC / 1.33 bar
18
35
9
Solid + Dry
134ºC / 2 bar
10
35
0
-
-
-
-
17. Start
1. Main switch
2. Open door indicator lamp.
3. Lack of water indicator lamp.
4. End cycle indicator lamp.
5. Liquid mode selector.
6. Solid mode selector.
7. Solid+Drying mode selector.
8. Temperature indicator display.
9. Temperature select pushbutton.
10. Parameter up pushbutton.
1 1. Parameter down pushbutton.
12. Drying time indicator.
13. Time indicator display.
14. Time pushbutton.
15. Start pushbutton.
16. Program indicator display.
17. Program pushbutton.
18. Stop pushbutton.
20. Alarm indicator lamp.
18. Top view
21. Water drainage valve
22. Steam drainage valve.
23. Internal tank filling
19. Rear view
24. Air filter.
25. Safety valve.
26. Drying control thermostat.
27. Safety thermostat.
28. Drainage valve.
29. Steam drainage valve
30. Water inlet.
31. Condensation outlet
32. Tank drainage.
20. Cleaning Procedure
NEVER use bleach, steel wool, a steel brush or anything
abrasive to scrub or clean the Chamber.
1. remove instruments and trays from the autoclave.
2. spread the contents of a packet in chamber evenly.
3. Select and start program No. 1 (without dry).
4. At the end of the cycle drain the water from the reservoir.
5. Fill the water reservoir with distilled water.
6. Repeat a sterilization cycle without Chamber Brite ™ powder,
to remove any excessive dirt in the pipes.
7. Select and start program No. 1 (without dry). When the cycle
is finished it will automatically exhaust.
8. At the end of the cycle drain the water from the reservoir.
9. Now autoclave ready to use.
21.
22. Preventive Maintenances
Quarterly
1. Clean all the filters.(Water, Air )
2. Fill the water level of feed tank up to maximum limit
3. Check that the manometers function as expected.
4. Check the accuracy of temperature reading.
5. Activate the safety valves manually to verify that they are operating well.
6. Conduct a general sterilization process write down
pressure, temperature, required times for completing each phase of the
cycle, put it on registration system.
7. Verify that it is functioning within tolerances defined by manufacturer
8. Check any abnormal sound in solenoids.
9. Clean the chamber and remove limescale if any, advice to use only
distilled water.
10. Check the earth leakage current, protective earth.
23. limescale
Limescale is the hard, off-
white, chalky deposit found in
kettles, hot-water boilers
Add little amount of any acid
heat the chamber.
Deposited CaCO3 will be
removed.
Then rinse off with plenty of
water.
24. Quality of Process
Sterilization process indicators
They check instrument’s control parameters like temperature, time
and pressure.
Chemical indicators
Adhesive tapes that go inside a
component or on packages.
Biological indicators
The spore indicator is placed in the
sterilizing load.
After the process, it is incubated,
analyzed and it is determined if the
cycle meets with the sterilization
requirements.