I am Abu Musa. This is my Internship Presentation. This is for partial fulfillment of the 4th-year final examination of the Department of Fisheries, University of Dhaka. This is based on my findings from one month of research on the Coxs Bazar coast. The research is done in the live feed lab of BFRI Cox's Bazar.

1. WELCOME TO
MY
INTERNSHIP PRESENTATION
Presented by
Abu Musa
Exam Roll: 2030
Registration no: 2015817772
Department of Fisheries
University of Dhaka
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2. 2
Topic

3. Summary
Introduction
Materials and Methods
Results
Conclusion and Recommendation
The Outline of Presentation
3

4.  pH, salinity, temperature and DO respectively in the study area were 6.5, 26.5 ppt, 30.7 ℃ and
3.4 ppm over the study period.
 The total number of identified genera of phytoplankton and zooplankton was 11.
 The density of plankton was 167 individuals per cubic meter seawater.
Summary
4

5. The small and microscopic organisms drifting or floating in the sea or fresh water, consisting chiefly of
diatoms, protozoans, small crustaceans, and the eggs and larval stages of larger animals.
-Oxford Dictionary
Definition of Plankton
Introduction
01 Phytoplankton
Phytoplankton are the autotrophic (self-
feeding) components of the plankton
community and a key part of ocean and
freshwater ecosystems
02
Zooplankton
zooplankton consisting of small animals and
the immature stages of larger animals.
5

6.  Live food organisms include all plants (phytoplankton) and animals
(zooplankton) which are used in finfish and shellfish larval rearing
system.
 Live foods are able to swim in water column and are available to fish
and shellfish larvae thereby stimulate the feeding response (David
2003)
 Phytoplankton comprises the base of the food chain in the marine
environment.
Introduction
Figure 1 The central role of microalgae in mariculture
(Brown et al., 1989). Source :FAO 1996
Plankton as live feed?
1. Brown, M.R. 1991. The amino acid and sugar composition of 16 species of microalgae used in mariculture. Aquaculture, 145: 79-99.
2. David, A. B., 2003. Status of marine aquaculture in relation to live prey: past, present and future. In: Live feeds in marine aquaculture Josianne, G. S., and Lesley, A.M.
(Eds.). Blackwell publishing, UK, pp. 1-16
6

7. Table-1: Culturable genera or species as live feed
7
Introduction
Diatoms Flagellates Green algae Rotifers Copepods
Skeletonema
costatum
Isochrysis
galbana
Chlorella spp. Brachionus
plicatilis
Acartia
Thalassiosira
pseudonana
Tetraselmis
suecica
Nannochloropsis
spp
B. rotundiformis Pseudodiaptomus
Chaetoceros
gracilis
Monochrysis
lutheri
Apocyclopus
C. calcitrans Calanus

8. Specific objectives
 To learn plankton biodiversity along Cox’s Bazar coast.
 To learn techniques of plankton identification
 To learn assessment of plankton population and water quality parameter.
 To acquire knowledge about culture system of live feed
Objective of The Study
Overall objective
The overall objective of this study is to understand and asses the current condition of
plankton biodiversity in the Bay of Bengal, Cox’s Bazar and Learning culture technique of
live feed.
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9. Bangladesh
Cox’s Bazar Sea
Beach
Bakkhali
Bangladesh
Fisheries Research
Institute, Marine
Fisheries &
Technology
Station, Cox’s
Bazar
Internship Period: 23/03/2021-08/03/2021 (14 days)
Materials and Methods
10
Study Area & Duration of Study:

10. pH Meter Slide
Seawater Refractometer
Vortex Machine
Incubator Micropipette Plankton Net Agar Compound Microscope Weight Machine
Materials used in the study
Autoclave Machine
11

11. Study Procedure
Sample collection
Sample are collected
from selected area
1
Recording
Physicochemical
Conditions of
Water
Physicochemical
Condition recorded
regularly
2
Identification of
Plankton
Identification are
done by
Compound
Microscope
3
Counting of
plankton
Counting plankton
using mathematical
formula
4
Stock Culture of
phytoplankton
A selected species
was cultured
5
12

12. Sample Collection
Sample collecting from Holiday Point
Sample collecting from Kolatoli Point
 Plankton samples were collected horizontally from four selected areas
using simple conical tow-net oblique, which mesh size is 40
micrometers.
 Sample were collected filtering 40 L seawater
 Sample was collected just before high tide.
 Sample collected from 2-meter depth of sea water.
13

13. sample under microscope
Observing sample
Identification of Plankton
14
 The identification was performed by using a compound microscope
(LEICA DM500 compound microscope).
 Depending on plankton size 10X, 40X and 100X magnification were
used.
 Identification was done with the help of following book: illustration of
the marine phytoplankton of Japan and the site diatoms.org.

14. 15
Plankton were counted using following formula:
No. individuals/ m3 =
𝑉1×𝐶
𝑉2×𝑉3
,
Where,
C = number of organisms counted,
V1 = volume of the concentrated sample (ml),
V2 = volume of the sample counted (ml),
V3 = volume of the filtered volume of water (m3)
Counting of plankton

15. Isolation of plankton
1
2
3
4
5
6
Isolation of
single cells
Serial dilution
Centrifuge
washing
technique
Preparation of
agar plat
Streak plating
technique
Enrichment
media

16. Solution (A)
1. Potassium Nitrate (KNO3) 100g
2. Sodium di-hydrogen orthophosphate
(NaH2PO4.2H2O)
20g
3. EDTA di-sodium salt (Na2EDTA) 45g
4. Boric Acid (H3BO3) 33.4g
5. Ferric Chloride (FeCl3) 1.3g
6. Manganous Chloride (MnCl2.2H2O) 0.36g
7. Distilled Water 1L
Solution (B)
1. Zinc Chloride (ZnCl2) 4.2g
2. Cobalt Chloride (CoCl2.6H2O) 4.0g
3. Copper Sulphate (CuSO4.5H2O) 4.0g
4. Ammonium molybdate ((NH4)6Mo7O24.4H2O) 1.8g
5. Distilled Water 1L
Solution (C)
1. Vitamin B1 (Thiamine) 2g
2. Vitamin B 12 (Cyanocobalamin) 100m
g
3. Distilled Water 1L
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Enrichment media
 To grow skeletonema Conway/walne’s medium was used.
The media is prepared in lab using Conway/walne’s
formula.
 The nutrient media were put in each of the sampling bottles
in following amount 1ml of each Solution A, 0.5ml of B and
0.1 ml of C into 1 L sea water.

17. Parameters Used value Optimum
Temperature (°C) 25±1 18-24
Salinity (g L-1)
22-24 20-24
Light intensity (Lux) 2000-5000 2500-5000
Photoperiod Hrs. (light: dark) 24:0. 24:0 (max.)
pH 7.2 8.0-8.5 18
Stock Culture of phytoplankton
 The culture media of phytoplankton was prepared by washing 100 mL of conical flask and sterilized by using
autoclave for 15 min at 121EC.
 Required amount of Conway/walne’s and F2 medium were added.

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Stock Culture of phytoplankton
Stock culture of Collected sample
Supplying nutrient media Culture Chamber
Isolation Unit
 The observation of culture done each day.
 After 2 days, when water colour was brown, the cell were counted by using a haemocytometer under
a compound micro-scope at 40X magnification

19. Culture Water Sterilization
Nutrient Enrichment
Inoculation Cell Count Stock Culture
Chlorination
Dechlorinated
Autoclave
Contaminated free live
organism
Wash by detergent
Wash by fresh water
Autoclave
Soaked in chlorine
Sodium nitrate, Sodium,
Hypophosphate,
Sodium silicate,
Ferric Chloride,
EDTA,
Thiamin,
Biotin
10% Inoculation
Using sprit lamp
Use 60% alcohol
Results (cell/ml)
Haemo-cytometer
Boiling
Removing bubble
Add Nutrient
Mixing sea water
and agar
Spreading species
20
Live feed Culture
Overall Procedure of Live feed Culture

20. Station pH Salinity Temperature DO
Laboni Point 6.5 32 25 3.7
Holiday Point 6.5 33 26 3.5
Kolatoli point 6.4 31 27 3.5
Fisheries
landing center,
Bakkhali
6.7 27 28 3.2
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Results
 In this study, physicochemical conditions of water were
analysed.
 The average pH value is 6.5.
 The average salinity is 30.7
 Average temperature value is 26.5
 Average DO is 3.4

21. 5
5.5
6
6.5
7
24-Feb 26-Feb 28-Feb 3-Mar 6-Mar
pH trend over the Study period
2
2.5
3
3.5
4
4.5
5
24-Feb 26-Feb 28-Feb 3-Mar 6-Mar
Trend of DO over the study period
22
Salinity and temperature
The pH was recorded each time after collecting sample from 4 different station and calculated average
value. The value of them shown in the chart-1
The DO of sea water was recorded each time after collecting sample. Calculation was done in lab. The
calculated value of DO is shown in the chart-2
Chart 1 pH trend over the Study period Chart 2 Trend of DO over the study period

22. 28
29
30
31
32
33
34
35
24-Feb 26-Feb 28-Feb 3-Mar 6-Mar
Salinity trend over the study
period
24
25
26
27
28
29
30
24-Feb 26-Feb 28-Feb 3-Mar 6-Mar
Temperature trend over the
study period
23
Salinity and temperature
 The salinity was also recorded using a digital refractometer each time after collecting sample from 4 different
station and calculated average value. The value of them shown in the chart-3
 The Temperature of sea water was recorded each time after collecting sample. Calculation was done in lab. The
calculated value of DO is shown in the chart-4
Chart 3 salinity trend over the study period
Chart 3 Temperature trend over the study period

23. Class Order Family Genus
Eustigmatophyceae Eustigmatales Monodopsidaceae Nannochloropsis
Chaetocerotaceae Chaetoceros
Coscinodiscophyceae Skeletonema
Cyanophyceae Dinophysiales Dinophysiaceae Dinophysis
Bacillariophyceae Naviculales Naviculaceae Navicula
Bacillariophyceae Naviculales Pleurosigmataceae Gyrosigma
Cyanophyceae Spirulinales Spirulinaceae Spirilunia
Coscinodiscophyceae Rhizosoleniales Rhizosoleniaaceae Rhizosolenia
Hexanauplia Calanoida Acartiidae Acartia
Trebouxiophyceae Chlorellales Chlorellaceae Chlorella
Chlorodendrophyceae Chlorodendrales Chlorodendraceae Tetraselmis spp 24
Plankton Community
The total number of identified genera of plankton was 11 and many more unidentified in the Bay of Bengal
in following table

24.  Recommendations
After collecting sample, proper treatment should give to avoid death of plankton.
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Conclusion and Recommendation
 Conclusion
The eleven genera had identified from the collected samples from mentioned coastal point. Among
them naocholoropsis , skeletonema, navivula and cholorella are important. Nannocholoropsis,
Cholorella. Skeletonema, Tetraselmis spp are used as live feed.

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