The document summarizes using short-read sequencing to characterize microbes in seawater. It discusses challenges like microbial diversity and unknown functions. The main objective was to characterize a community from a disturbed environment using metagenomics. Abundant microbes like Candidatus Pelagibacter ubique and Nitrosopumilus martimus were identified. Archaeal ammonia monooxygenase genes were more abundant than bacterial ones, suggesting archaea dominate ammonia oxidation in this environment. More cultured representatives are needed to fully annotate metagenomes.