Aliaksei Yantsevich, researcher Yaroslav Dichenko, researcher Institute of Bioorganic Chemistry, NAS of Belarus SciHachathon Challenges: http://sciencehit.by/hackathon

1. Artificial gene synthesis
Institute of Bioorganic Chemistry, NAS of Belarus
Aliaksei Yantsevich, researcher
Yaroslav Dichenko, researcher

2. EPIGRAPH
DNA is like a computer program
but far, far more advanced than
any software ever created.
- Bill Gates -

3. What is a GENE
Gene – autonomous module of hereditary information.
Information
DNA (4 types of elements):
A, T, G, C
Realization
Protein (20 types of elements):
G, L, Y, S, E, Q, D, N, F, A,
K, R, H, C, V, P, W, I, M, T
Genetic code properties:
UAA=UUG=CUU=CUC=CUA=CUG=L

4. What is artificial (synthetic) gene
Synthetic biology –
development of biological systems
with given properties and functions,
which have no analogues in
nature.
Synthetic gene –
DNA sequence, obtained de novo by
a complex of chemical, physical and
enzymatic methods.

5. Genes application

6. Artificial genes
for ordinary person???
Stive, but why would an
ordinary person have a
computer.
2017 г.
1976 г.

7. But for now:

8. Perhaps,
in 20 years on Mars…

9. What is the price for a gene

10. World market
 Equipment for combinatorial chemistry:
• 5.0 million $ (2012 г.)
Oligonucleotides:
• 500 million $ (2006 г.)
• 776 million $ (2010 г.)
Synthetic biology products:
0.6 billion $ (2006 г.)
1.1 billion $ (2010 г.)
1.6 billion $ (2011 г.)
2.4 billion $ (2013 г.)
10.8 billion $ (2016 г.)

11. Synthetic biology market structure

12. How to print genes
(DNA-printing)
Oligonucleotides
synthesis
Planning Gene seq. optimization
Gene assemblyGene cloning DNA purification
Gene seq. fragmentation
5' 3'
5'
3'
3'
5'
5'3'
Oligonucleotides
Gene

13. It would be good to have
Gene Synthesizing Black Box
Episode1
“IHaveAnIdea.”
Episode2
(final)
“IHaveTheGene.”

14. If it works…
………2021

15. What is next step?
Lab-On-Chip gene synthesizer

16. And, finally:
………2025

17. Current problems
 Last 30 years equipment and software were subjected to
minor changes and this is the reason for synthetic gene
price retention.
 Software to control equipment:
• desk-top programs;
• closely connected to computer architecture;
• unstable work.
 There is no full cycle artificial gene synthesizer on world
market.

18. Aim of the project
 Development of the prototype for full cycle automatic
artificial gene synthesis system, controlled by cross-
platform web-software.
 Development of first IoSE (Internet of Scientific Equipment)
system
 Subtasks:
• Gene sequence optimization
• Gene sequence fragmentation
• Calculation of synthetic program
• Oligonucleotide synthesis
• Oligonucleotide preparation/purification
• Gene assembly

19. How to realize prototype:
general structure of system
Hardware
Interface 01
.
.
.
.
.
Deep tuning and
configuration
Hardware
interface 02
Emdedded-computer
1. Control
2. Web-server
3. WIFI hot-spot
REST API
Oligonucleotide synthesis
hardware
(CDS ABI 380)
Additional equipment -
gene synthesis
(solenoid valves, sensors, pumps,
thermostats etc.
Cloud service
1.Gene sequence fragmentation
2.Synthesis optimization
3.Additional calculations
4.Process control
5.System monitoring

20. Software structure
Module Function
Gene editor Optimization of gene sequences for synthesis.
Function editor Function – state of the equipment during synthetic cycle.
Synthetic cycle editor Cycle – sequence of functions, activated during stated
period of time.
Synthetic method editor Synthetic method – sequence of equipment states
intended for gene synthesis.
Diagnostics Connection check, status check for different modules.
Direct control Control of individual hardware modules: on-off, set/change
desired state.
System monitor Process monitoring, system monitoring.

21. In stock
Interest
Old oligosynthesizer
Odds and ends
Raspberry PiAim
Time
DIY parts and components
Small team

22. Necessary:
Financial support for development and prototyping
JS-programmer
UI/UX designer
Anyone:
who feels that interested and
who can and wants to contribute to the project

23. The END
Thank you for your
attention
Aliaksei Yantsevich, al.yantsevich@gmail.com
Yaroslav Dichenko, yaroslavd@tut.by