This double-blind, placebo-controlled trial explored the safety and potential efficacy of hyperimmune caprine serum (AIMSPRO) in 20 patients with established diffuse cutaneous systemic sclerosis (SSc) over 26 weeks. The trial found no safety concerns with AIMSPRO. Patients receiving AIMSPRO showed a mean decrease in modified Rodnan Skin Score compared to an increase in the placebo group. Levels of PIIINP, a biomarker of fibrosis, increased less in the AIMSPRO group. The results support the safety of AIMSPRO and suggest it may provide clinical benefit for skin disease in SSc.
Convalescent Plasma and COVID-19: Ancient Therapy Re-emergedasclepiuspdfs
Convalescent plasma has again re-emerged as a therapy during coronavirus disease (COVID-19) outbreaks currently use as a prophylactic or an interventional treatment in infected patients. Convalescent plasma has been used in the 20th century confronting different infectious diseases where there was no other therapy available. Conceivably, this convalescent plasma therapy tends to be proving a game-changing treatment in some COVID-19 patients and could support treatment, in addition to the current interventions before other developed therapies are available for the population.
A Possible Role of Rosmarinic Acid against CD2 Associated Protein for the Tre...YogeshIJTSRD
Multiple sclerosis is a chronic inflammatory neurodegenerative disorder which directly affects Central Nervous System CNS . People with MS suffer with an episodic reversible memory loss during the initial stages and later it leads to the neurological deterioration. Number of research and studies has been done on the natural compounds and phytochemical compounds in order to develop the particular drug for the treatment of MS in vivo andin vitro. The present study focuses on the inhibitory effect of Rosmarinic acid against the effect of CD2 Associated protein with the help of Molecular Docking. Molecular Docking basically screens the ligand and the target protein and shows the interaction between them on the basis of the minimum binding affinities and drug likeliness properties. In our research, docking was performed between CD2 Associated protein and selected ligands with the help of docking software. Ligands were selected on the basis of their minimum Binding affinities and finally by their drug likeliness properties. Rosmarinic acid BA 5.6 was the resultant ligand of our recent study. It showed the perfect interaction with CD2 Associated protein. Therefore, we may conclude that Rosmarinic acid may act as a compound which may be used as a drug for the treatment of multiple sclerosis fromfurther in vitro and in vivostudies in future. Jitin Kumar | Tejaswee Anand | Ritika Sharma | Noopur Khare | Abhimanyu Kumar Jha | Yamini Dixit "A Possible Role of Rosmarinic Acid against CD2-Associated Protein for the Treatment of Multiple Sclerosis through in Silico Approach" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd44979.pdf Paper URL: https://www.ijtsrd.com/biological-science/biotechnology/44979/a-possible-role-of-rosmarinic-acid-against-cd2associated-protein-for-the-treatment-of-multiple-sclerosis-through-in-silico-approach/jitin-kumar
—In the Indian sub-continent, first isolation of the chikungunya virus was done in Kolkata during 1963. During 2006 reports of large scale outbreaks in several parts of India have confirmed the re-emergence of this virus in the country. Since the incidence of this disease is increasing. So a retrospective analysis of laboratory confirmed chikungunya patients admitted to pediatric ward was done to study biochemical profile of chikungunya fever in children. Total 51 children were laboratory confirmed for chickungunya, 36 of them had isolated chikungunya infection. Male/female ratio of isolated chikungunya was 2.6:1. Fever was invariably present, associated constitutional symptoms consisted of skin rash, vomiting, diarrhea, pain abdomen, cough, corrhyza, myalgia and bleeding manifestations. The most characteristic feature of the infections in infants was skin manifestations in form of symmetrical superficial vesiculobullous lesions & maculopapular erythematous rash. Nine patients (25%) had neurological manifestations. Joint pain was present in only three patients but none had arthritis. Most common hematological abnormality revealed thrombocytopenia in 39% cases. There was mild to moderate elevation of liver enzymes in 13 patients (36%). Average length of hospital stay was 5.1 days. Thirty four patients recovered completely & two left against medical advise. It is concluded from this study that skin manifestations and neurological manifestations are common in younger age group apart from other constitutional symptoms. Arthralgia and chronic polyarthritis is rare in this age group as found in adults.
Convalescent Plasma and COVID-19: Ancient Therapy Re-emergedasclepiuspdfs
Convalescent plasma has again re-emerged as a therapy during coronavirus disease (COVID-19) outbreaks currently use as a prophylactic or an interventional treatment in infected patients. Convalescent plasma has been used in the 20th century confronting different infectious diseases where there was no other therapy available. Conceivably, this convalescent plasma therapy tends to be proving a game-changing treatment in some COVID-19 patients and could support treatment, in addition to the current interventions before other developed therapies are available for the population.
A Possible Role of Rosmarinic Acid against CD2 Associated Protein for the Tre...YogeshIJTSRD
Multiple sclerosis is a chronic inflammatory neurodegenerative disorder which directly affects Central Nervous System CNS . People with MS suffer with an episodic reversible memory loss during the initial stages and later it leads to the neurological deterioration. Number of research and studies has been done on the natural compounds and phytochemical compounds in order to develop the particular drug for the treatment of MS in vivo andin vitro. The present study focuses on the inhibitory effect of Rosmarinic acid against the effect of CD2 Associated protein with the help of Molecular Docking. Molecular Docking basically screens the ligand and the target protein and shows the interaction between them on the basis of the minimum binding affinities and drug likeliness properties. In our research, docking was performed between CD2 Associated protein and selected ligands with the help of docking software. Ligands were selected on the basis of their minimum Binding affinities and finally by their drug likeliness properties. Rosmarinic acid BA 5.6 was the resultant ligand of our recent study. It showed the perfect interaction with CD2 Associated protein. Therefore, we may conclude that Rosmarinic acid may act as a compound which may be used as a drug for the treatment of multiple sclerosis fromfurther in vitro and in vivostudies in future. Jitin Kumar | Tejaswee Anand | Ritika Sharma | Noopur Khare | Abhimanyu Kumar Jha | Yamini Dixit "A Possible Role of Rosmarinic Acid against CD2-Associated Protein for the Treatment of Multiple Sclerosis through in Silico Approach" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd44979.pdf Paper URL: https://www.ijtsrd.com/biological-science/biotechnology/44979/a-possible-role-of-rosmarinic-acid-against-cd2associated-protein-for-the-treatment-of-multiple-sclerosis-through-in-silico-approach/jitin-kumar
—In the Indian sub-continent, first isolation of the chikungunya virus was done in Kolkata during 1963. During 2006 reports of large scale outbreaks in several parts of India have confirmed the re-emergence of this virus in the country. Since the incidence of this disease is increasing. So a retrospective analysis of laboratory confirmed chikungunya patients admitted to pediatric ward was done to study biochemical profile of chikungunya fever in children. Total 51 children were laboratory confirmed for chickungunya, 36 of them had isolated chikungunya infection. Male/female ratio of isolated chikungunya was 2.6:1. Fever was invariably present, associated constitutional symptoms consisted of skin rash, vomiting, diarrhea, pain abdomen, cough, corrhyza, myalgia and bleeding manifestations. The most characteristic feature of the infections in infants was skin manifestations in form of symmetrical superficial vesiculobullous lesions & maculopapular erythematous rash. Nine patients (25%) had neurological manifestations. Joint pain was present in only three patients but none had arthritis. Most common hematological abnormality revealed thrombocytopenia in 39% cases. There was mild to moderate elevation of liver enzymes in 13 patients (36%). Average length of hospital stay was 5.1 days. Thirty four patients recovered completely & two left against medical advise. It is concluded from this study that skin manifestations and neurological manifestations are common in younger age group apart from other constitutional symptoms. Arthralgia and chronic polyarthritis is rare in this age group as found in adults.
Journal club presentation: by RxVichuZ!! ;)RxVichuZ
My 97th powerpoint... deals with the comparative study of efficacy of piperacillin-tazobactam, as compared to meropenem in the treatment of ESBL(Extended spectrum beta-lactamases) infections.
A summarized insight has been provided, using research article from JAMA.
SEPSIS IS MOST FATAL DISEASE WORLD WIDE. EARLY DETECTION OR PREDICTION OF SEPSIS IS A CHALLENGE
SEPSIS BIOMARKERS ARE OUR WEAPON TO EARLY DETECT SEPSIS. WE HAVE TO UNDERSTAND IT WELL
Presented by Jane Dematte, MD at the Scleroderma Patient Education Conference hosted by the Scleroderma Foundation on Saturday, October 12, 2019 in Chicago, IL
Etiologia de la celulitis y Predicción clínica de la enfermedad Estreptocócic...Alex Castañeda-Sabogal
Etiologia de la celulitis. Estudio prospectivo y predicción clínica de la infeccion por Estreptococcus basado en la frecuencia encontrada de las especies de estreptococo
ABSTRACT- The treatment of carbuncle is early administration of antibiotics and surgery. The commonest surgical approach is Saucerization and Incision & Drainage (I&D). Two cases are presented here, one underwent Saucerization and then primary split thickness skin grafting. Another un-derwent I&D for her carbuncle. They were followed up for 8 weeks to assess their outcome. Saucerization produced the shortest length of hospital stay while I&D resulted in shortest wound healing. As a new modality of treatment now-a-days two new modalities gaining popularity for better cosmetic purpose: primary split thickness skin grafting & transposition of local skin/musculocutaneous flap.
Keywords: carbuncle, surgery, good glycemic control
To Assess the Severity and Mortality among Covid 19 Patients after Having Vac...YogeshIJTSRD
The severity and mortality of COVID 19 cases has been associated with the Three category such as vaccination status, severity of disease and outcome. Objective presently study was aimed to assess the severity and mortality among covid 19 patients. Methods Using simple lottery random method 100 samples were selected. From these 100 patients, 50 patients were randomly assigned to case group and 50 patients in control group after informed consents of relative obtained. Patients in the case group who being died after got COVID 19 whereas 50 patients in the control group participated who were survive after got infected from COVID 19 patients. Result It has three categories such as a Vaccination status For the vaccination status we have seen 59 patients were not vaccinated and 41 patients was vaccinated out of 100. b Incidence There were 41 patients were vaccinated whereas 59 patients were not vaccinated. c Severity In the case of mortality we selected 50 patients who were died from the Corona and I got to know that out of 50 patients there were 12 24 patients were vaccinated whereas 38 76 patients were non vaccinated. Although for the 50 control survival group total 29 58 patients were vaccinated and 21 42 patients was not vaccinated all graph start. Conclusion we have find out that those people who got vaccinated were less infected and mortality rate very low. Prof. (Dr) Binod Kumar Singh | Dr. Saroj Kumar | Ms. Anuradha Sharma "To Assess the Severity and Mortality among Covid-19 Patients after Having Vaccinated: A Retrospective Study" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45065.pdf Paper URL: https://www.ijtsrd.com/other-scientific-research-area/other/45065/to-assess-the-severity-and-mortality-among-covid19-patients-after-having-vaccinated-a-retrospective-study/prof-dr-binod-kumar-singh
Highly active antiretroviral therapyi (HAART), a combination of drugs (lamivudine, zidovudine and nevirapine) used for pre-exposure prophylaxis and management of Human Immunodeficiency Virus infection in sub-Saharan Africa. The objective of this research work was to investigate the potential Ameliorative effect of neuroviteon on HAART induced toxicity on the cerebellum. Thirty two Wistar rats were divided into 4 groups of 8 rats each. Group A served as the control, while group B were administered with 9.28 mg/kg of HAART, group C received 9.28 mg/kg of HAART and 0.07mg/kg of folic acid and group D received 0.07mg/kg folic acid. Drugs were administered twice daily for 30 days after which neurobehavioural test of open field maze was perform. The rats were then sacrificed and their cerebellum harvested, processed and stained using haematoxylin and eosin method and nuro-fillament (NF) immunochemistry method. The slides were viewed under light microscope. Results showed a significant reduction in the brain to body weight index between the HAART group and the control and folic acid group. There was significant reduction in locomotor activity following administration of HAART to the animals compared with control, there were also significant reduction in rearing frequency , walling frequency and freezing duration, with a significant increased in freezing duration in the HAART treatment group. The freezing frequency, central line crossing and grooming frequency were not significantly different. The cerebella were affected with mild to moderate shrinkage of pyramidal cells and distortion of the granular cells. There was increased expression of NF in the HAART group compared to controls. HAART affects the weight, histology of the cerebellum and neurobehaviour. Neurovite has the potential of ameliorating the histological distortion and may be beneficial to people taking HAART.
1 gastrointestinal manifestations of systemic sclerosismaushard
"Gastrointestinal Manifestations of Systemic Sclerosis" presentation by Dr. Harald Schoeppner MD PhD. for the 12th annual Cheri Woo Scleroderma Education Seminar on March 9, 2013 hosted by Oregon Chapter of the Scleroderma Foundation.
Journal club presentation: by RxVichuZ!! ;)RxVichuZ
My 97th powerpoint... deals with the comparative study of efficacy of piperacillin-tazobactam, as compared to meropenem in the treatment of ESBL(Extended spectrum beta-lactamases) infections.
A summarized insight has been provided, using research article from JAMA.
SEPSIS IS MOST FATAL DISEASE WORLD WIDE. EARLY DETECTION OR PREDICTION OF SEPSIS IS A CHALLENGE
SEPSIS BIOMARKERS ARE OUR WEAPON TO EARLY DETECT SEPSIS. WE HAVE TO UNDERSTAND IT WELL
Presented by Jane Dematte, MD at the Scleroderma Patient Education Conference hosted by the Scleroderma Foundation on Saturday, October 12, 2019 in Chicago, IL
Etiologia de la celulitis y Predicción clínica de la enfermedad Estreptocócic...Alex Castañeda-Sabogal
Etiologia de la celulitis. Estudio prospectivo y predicción clínica de la infeccion por Estreptococcus basado en la frecuencia encontrada de las especies de estreptococo
ABSTRACT- The treatment of carbuncle is early administration of antibiotics and surgery. The commonest surgical approach is Saucerization and Incision & Drainage (I&D). Two cases are presented here, one underwent Saucerization and then primary split thickness skin grafting. Another un-derwent I&D for her carbuncle. They were followed up for 8 weeks to assess their outcome. Saucerization produced the shortest length of hospital stay while I&D resulted in shortest wound healing. As a new modality of treatment now-a-days two new modalities gaining popularity for better cosmetic purpose: primary split thickness skin grafting & transposition of local skin/musculocutaneous flap.
Keywords: carbuncle, surgery, good glycemic control
To Assess the Severity and Mortality among Covid 19 Patients after Having Vac...YogeshIJTSRD
The severity and mortality of COVID 19 cases has been associated with the Three category such as vaccination status, severity of disease and outcome. Objective presently study was aimed to assess the severity and mortality among covid 19 patients. Methods Using simple lottery random method 100 samples were selected. From these 100 patients, 50 patients were randomly assigned to case group and 50 patients in control group after informed consents of relative obtained. Patients in the case group who being died after got COVID 19 whereas 50 patients in the control group participated who were survive after got infected from COVID 19 patients. Result It has three categories such as a Vaccination status For the vaccination status we have seen 59 patients were not vaccinated and 41 patients was vaccinated out of 100. b Incidence There were 41 patients were vaccinated whereas 59 patients were not vaccinated. c Severity In the case of mortality we selected 50 patients who were died from the Corona and I got to know that out of 50 patients there were 12 24 patients were vaccinated whereas 38 76 patients were non vaccinated. Although for the 50 control survival group total 29 58 patients were vaccinated and 21 42 patients was not vaccinated all graph start. Conclusion we have find out that those people who got vaccinated were less infected and mortality rate very low. Prof. (Dr) Binod Kumar Singh | Dr. Saroj Kumar | Ms. Anuradha Sharma "To Assess the Severity and Mortality among Covid-19 Patients after Having Vaccinated: A Retrospective Study" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-5 , August 2021, URL: https://www.ijtsrd.com/papers/ijtsrd45065.pdf Paper URL: https://www.ijtsrd.com/other-scientific-research-area/other/45065/to-assess-the-severity-and-mortality-among-covid19-patients-after-having-vaccinated-a-retrospective-study/prof-dr-binod-kumar-singh
Highly active antiretroviral therapyi (HAART), a combination of drugs (lamivudine, zidovudine and nevirapine) used for pre-exposure prophylaxis and management of Human Immunodeficiency Virus infection in sub-Saharan Africa. The objective of this research work was to investigate the potential Ameliorative effect of neuroviteon on HAART induced toxicity on the cerebellum. Thirty two Wistar rats were divided into 4 groups of 8 rats each. Group A served as the control, while group B were administered with 9.28 mg/kg of HAART, group C received 9.28 mg/kg of HAART and 0.07mg/kg of folic acid and group D received 0.07mg/kg folic acid. Drugs were administered twice daily for 30 days after which neurobehavioural test of open field maze was perform. The rats were then sacrificed and their cerebellum harvested, processed and stained using haematoxylin and eosin method and nuro-fillament (NF) immunochemistry method. The slides were viewed under light microscope. Results showed a significant reduction in the brain to body weight index between the HAART group and the control and folic acid group. There was significant reduction in locomotor activity following administration of HAART to the animals compared with control, there were also significant reduction in rearing frequency , walling frequency and freezing duration, with a significant increased in freezing duration in the HAART treatment group. The freezing frequency, central line crossing and grooming frequency were not significantly different. The cerebella were affected with mild to moderate shrinkage of pyramidal cells and distortion of the granular cells. There was increased expression of NF in the HAART group compared to controls. HAART affects the weight, histology of the cerebellum and neurobehaviour. Neurovite has the potential of ameliorating the histological distortion and may be beneficial to people taking HAART.
1 gastrointestinal manifestations of systemic sclerosismaushard
"Gastrointestinal Manifestations of Systemic Sclerosis" presentation by Dr. Harald Schoeppner MD PhD. for the 12th annual Cheri Woo Scleroderma Education Seminar on March 9, 2013 hosted by Oregon Chapter of the Scleroderma Foundation.
Cardiovascular Manifestations, Systemic Sclerosis by Dr. Jonathan R. Lindner MDmaushard
Presentation by Dr. Jonathan R. Lindner MD at the 13th Annual Cheri Woo Scleroderma Education Seminar on March 8, 2014 in Portland, Oregon. The seminar is a free public service hosted by the Oregon Chapter of the Scleroderma Foundation.
Skin Manifestations of Scleroderma, by Dr. Lorinda Chung MD maushard
Keynote presentation by Dr. Lorinda Chung MD at March 9, 2013 Cheri Woo Scleroderma Education Seminar in Tualatin, OR hosted by Oregon Chapter of the Scleroderma Foundation.
The Role of Radiotherapy in the Treatment of Early Stage Ocular Marginal Zone...daranisaha
To evaluate the benefit of radiotherapy, compared with other treatment in ocular marginal zone lymphoma, retrospectively we analyzed our experience, with the end-points: efficacy, measured for complete response, Progression-Free Survival (PFS) and Overall Survival
Abnormal Sodium and Chlorine Level Is Associated With Prognosis of Lung Cance...semualkaira
The imbalance of sodium and chloride ions occurs frequently in patients with lung cancer. However, the correlation between ion concentration change and patients prognosis have not been studied thoroughly. Our research will fill the gap, especially for high ion concentration.
Abnormal Sodium and Chlorine Level Is Associated With Prognosis of Lung Cance...semualkaira
The imbalance of sodium and chloride ions occurs frequently in patients with lung cancer. However, the correlation between ion concentration change and patients prognosis have not been studied thoroughly. Our research will fill the gap, especially for high ion concentration.
Abnormal Sodium and Chlorine Level Is Associated With Prognosis of Lung Cance...JohnJulie1
The imbalance of sodium and chloride ions occurs frequently in patients with lung cancer. However, the correlation between ion concentration change and patients prognosis have not been studied thoroughly. Our research will fill the gap, especially for high ion concentration.
Abnormal Sodium and Chlorine Level Is Associated With Prognosis of Lung Cance...daranisaha
The imbalance of sodium and chloride ions occurs frequently in patients with lung cancer. However, the correlation between ion concentration change and patients prognosis have not been studied thoroughly. Our research will fill the gap, especially for high ion concentration.
Abstract—In Italy the hydatid disease is more prevalent and new cases are highlighted more frequently in Sicily, Sardinia, (Italy). Aim of this study is to put the indication in search of iaditea nature in both spleen swelling and muscle tendon.
Material and Method Patients observed during the period 2007-2009 at the Surgical Clinic III and Digestive Surgery, Policlinico G Rodolico were explored for Hydatid cyste at various sites. Diagnosis of cysts ecchinococcus occurred primarily for various four reasons either for compression of bodies involved or for eosinophilia or for instrumental investigation or for anaphylactic reaction to rupture of cysts. Biological diagnosis is based on serology rather than isolation of the parasite (indirect diagnosis);
Results Patients attended during the period 2007-2009 Hydatid cyst was found in 0.5% of all cases in liver along with 4 in the lung, 3 in splenic, 2 in the mammary and 2 in the chest wall No 2. The Surgical treatment with the complete removal of the cyst with a satisfactory postoperative course in the absence of cases of relapse of the disease and by following the therapeutic act, the assumption of mebendazole 50mg / kg / day for 3 weeks at a dose of 400mg for 4 months
Conclusions There is a need to define diagnostic methods with high specificity and sensitivity, which can provide a valid diagnostic aid for the cases clinically difficult to diagnose. And the final diagnosis must then also be based on the development of immunological methods that allow the determination of specific antibodies in the serum and their titration and / or the circulating antigen determination.
Title: Sense of Taste
Presenter: Dr. Faiza, Assistant Professor of Physiology
Qualifications:
MBBS (Best Graduate, AIMC Lahore)
FCPS Physiology
ICMT, CHPE, DHPE (STMU)
MPH (GC University, Faisalabad)
MBA (Virtual University of Pakistan)
Learning Objectives:
Describe the structure and function of taste buds.
Describe the relationship between the taste threshold and taste index of common substances.
Explain the chemical basis and signal transduction of taste perception for each type of primary taste sensation.
Recognize different abnormalities of taste perception and their causes.
Key Topics:
Significance of Taste Sensation:
Differentiation between pleasant and harmful food
Influence on behavior
Selection of food based on metabolic needs
Receptors of Taste:
Taste buds on the tongue
Influence of sense of smell, texture of food, and pain stimulation (e.g., by pepper)
Primary and Secondary Taste Sensations:
Primary taste sensations: Sweet, Sour, Salty, Bitter, Umami
Chemical basis and signal transduction mechanisms for each taste
Taste Threshold and Index:
Taste threshold values for Sweet (sucrose), Salty (NaCl), Sour (HCl), and Bitter (Quinine)
Taste index relationship: Inversely proportional to taste threshold
Taste Blindness:
Inability to taste certain substances, particularly thiourea compounds
Example: Phenylthiocarbamide
Structure and Function of Taste Buds:
Composition: Epithelial cells, Sustentacular/Supporting cells, Taste cells, Basal cells
Features: Taste pores, Taste hairs/microvilli, and Taste nerve fibers
Location of Taste Buds:
Found in papillae of the tongue (Fungiform, Circumvallate, Foliate)
Also present on the palate, tonsillar pillars, epiglottis, and proximal esophagus
Mechanism of Taste Stimulation:
Interaction of taste substances with receptors on microvilli
Signal transduction pathways for Umami, Sweet, Bitter, Sour, and Salty tastes
Taste Sensitivity and Adaptation:
Decrease in sensitivity with age
Rapid adaptation of taste sensation
Role of Saliva in Taste:
Dissolution of tastants to reach receptors
Washing away the stimulus
Taste Preferences and Aversions:
Mechanisms behind taste preference and aversion
Influence of receptors and neural pathways
Impact of Sensory Nerve Damage:
Degeneration of taste buds if the sensory nerve fiber is cut
Abnormalities of Taste Detection:
Conditions: Ageusia, Hypogeusia, Dysgeusia (parageusia)
Causes: Nerve damage, neurological disorders, infections, poor oral hygiene, adverse drug effects, deficiencies, aging, tobacco use, altered neurotransmitter levels
Neurotransmitters and Taste Threshold:
Effects of serotonin (5-HT) and norepinephrine (NE) on taste sensitivity
Supertasters:
25% of the population with heightened sensitivity to taste, especially bitterness
Increased number of fungiform papillae
Anti ulcer drugs and their Advance pharmacology ||
Anti-ulcer drugs are medications used to prevent and treat ulcers in the stomach and upper part of the small intestine (duodenal ulcers). These ulcers are often caused by an imbalance between stomach acid and the mucosal lining, which protects the stomach lining.
||Scope: Overview of various classes of anti-ulcer drugs, their mechanisms of action, indications, side effects, and clinical considerations.
MANAGEMENT OF ATRIOVENTRICULAR CONDUCTION BLOCK.pdfJim Jacob Roy
Cardiac conduction defects can occur due to various causes.
Atrioventricular conduction blocks ( AV blocks ) are classified into 3 types.
This document describes the acute management of AV block.
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Report Back from SGO 2024: What’s the Latest in Cervical Cancer?bkling
Are you curious about what’s new in cervical cancer research or unsure what the findings mean? Join Dr. Emily Ko, a gynecologic oncologist at Penn Medicine, to learn about the latest updates from the Society of Gynecologic Oncology (SGO) 2024 Annual Meeting on Women’s Cancer. Dr. Ko will discuss what the research presented at the conference means for you and answer your questions about the new developments.
Flu Vaccine Alert in Bangalore Karnatakaaddon Scans
As flu season approaches, health officials in Bangalore, Karnataka, are urging residents to get their flu vaccinations. The seasonal flu, while common, can lead to severe health complications, particularly for vulnerable populations such as young children, the elderly, and those with underlying health conditions.
Dr. Vidisha Kumari, a leading epidemiologist in Bangalore, emphasizes the importance of getting vaccinated. "The flu vaccine is our best defense against the influenza virus. It not only protects individuals but also helps prevent the spread of the virus in our communities," he says.
This year, the flu season is expected to coincide with a potential increase in other respiratory illnesses. The Karnataka Health Department has launched an awareness campaign highlighting the significance of flu vaccinations. They have set up multiple vaccination centers across Bangalore, making it convenient for residents to receive their shots.
To encourage widespread vaccination, the government is also collaborating with local schools, workplaces, and community centers to facilitate vaccination drives. Special attention is being given to ensuring that the vaccine is accessible to all, including marginalized communities who may have limited access to healthcare.
Residents are reminded that the flu vaccine is safe and effective. Common side effects are mild and may include soreness at the injection site, mild fever, or muscle aches. These side effects are generally short-lived and far less severe than the flu itself.
Healthcare providers are also stressing the importance of continuing COVID-19 precautions. Wearing masks, practicing good hand hygiene, and maintaining social distancing are still crucial, especially in crowded places.
Protect yourself and your loved ones by getting vaccinated. Together, we can help keep Bangalore healthy and safe this flu season. For more information on vaccination centers and schedules, residents can visit the Karnataka Health Department’s official website or follow their social media pages.
Stay informed, stay safe, and get your flu shot today!
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...Oleg Kshivets
RESULTS: Overall life span (LS) was 2252.1±1742.5 days and cumulative 5-year survival (5YS) reached 73.2%, 10 years – 64.8%, 20 years – 42.5%. 513 LCP lived more than 5 years (LS=3124.6±1525.6 days), 148 LCP – more than 10 years (LS=5054.4±1504.1 days).199 LCP died because of LC (LS=562.7±374.5 days). 5YS of LCP after bi/lobectomies was significantly superior in comparison with LCP after pneumonectomies (78.1% vs.63.7%, P=0.00001 by log-rank test). AT significantly improved 5YS (66.3% vs. 34.8%) (P=0.00000 by log-rank test) only for LCP with N1-2. Cox modeling displayed that 5YS of LCP significantly depended on: phase transition (PT) early-invasive LC in terms of synergetics, PT N0—N12, cell ratio factors (ratio between cancer cells- CC and blood cells subpopulations), G1-3, histology, glucose, AT, blood cell circuit, prothrombin index, heparin tolerance, recalcification time (P=0.000-0.038). Neural networks, genetic algorithm selection and bootstrap simulation revealed relationships between 5YS and PT early-invasive LC (rank=1), PT N0—N12 (rank=2), thrombocytes/CC (3), erythrocytes/CC (4), eosinophils/CC (5), healthy cells/CC (6), lymphocytes/CC (7), segmented neutrophils/CC (8), stick neutrophils/CC (9), monocytes/CC (10); leucocytes/CC (11). Correct prediction of 5YS was 100% by neural networks computing (area under ROC curve=1.0; error=0.0).
CONCLUSIONS: 5YS of LCP after radical procedures significantly depended on: 1) PT early-invasive cancer; 2) PT N0--N12; 3) cell ratio factors; 4) blood cell circuit; 5) biochemical factors; 6) hemostasis system; 7) AT; 8) LC characteristics; 9) LC cell dynamics; 10) surgery type: lobectomy/pneumonectomy; 11) anthropometric data. Optimal diagnosis and treatment strategies for LC are: 1) screening and early detection of LC; 2) availability of experienced thoracic surgeons because of complexity of radical procedures; 3) aggressive en block surgery and adequate lymph node dissection for completeness; 4) precise prediction; 5) adjuvant chemoimmunoradiotherapy for LCP with unfavorable prognosis.
Lung Cancer: Artificial Intelligence, Synergetics, Complex System Analysis, S...
Treatment of diffuse systemic sclerosis with AIMSPRO
1. ARD Online First, published on September 25, 2013 as 10.1136/annrheumdis-2013-203674
Clinical and epidemiological research
EXTENDED REPORT
Treatment of diffuse systemic sclerosis
with hyperimmune caprine serum (AIMSPRO):
a phase II double-blind placebo-controlled trial
N P Quillinan,1 D McIntosh,2 J Vernes,3 S Haq,2 C P Denton1
Handling editor Tore K Kvien
1
Centre for Rheumatology, UCL
Medical School, Royal Free
Campus, London, UK
2
Daval International Ltd.,
Eastbourne, East Sussex, UK
3
School of Mathematics,
Statistics and Actuarial Science,
The University of Kent,
Canterbury, UK
Correspondence to
Professor C P Denton,
Centre for Rheumatology and
Connective Tissue Diseases,
Royal Free Hospital and UCL
Medical School, Pond Street,
London NW3 2QG, UK;
c.denton@ucl.ac.uk
Eudract No. 2007-003122-24
ClinTrials.gov No.
NCT00769028
Received 24 March 2013
Revised 30 July 2013
Accepted 8 September 2013
ABSTRACT
Objective The primary objective of the study was to
explore safety and tolerability of hyperimmune caprine
serum (AIMSPRO) in established diffuse cutaneous
systemic sclerosis (SSc). Secondary objectives included
assessment of potential efficacy and biological activity
and exploration of candidate biomarkers.
Methods This was a double-blind parallel group
randomised placebo-controlled clinical trial. After
informed consent 20 patients with established diffuse
cutaneous SSc of greater than 3 years duration not
receiving immunosuppressive therapy were randomised
to receive either active (n=10) or placebo formulation
(n=10) by subcutaneous twice weekly injection over
26 weeks. Clinical assessments were evaluated over
26 weeks.
Results There were no safety concerns during this
study. Frequency of adverse events was not different
between active and placebo groups. Mean modified
Rodnan Skin Score (mRSS) fell by 1.4±4.7 units with
active treatment but increased by 2.1±6.4 units on
placebo when baseline values were compared with
26 weeks and responder analysis showed clinically
meaningful improvement in mRSS at 26 weeks in 5
(50%) of actively treated patients compared with 1
(10%) in the control group ( p=0.062). PIIINP (mg/L)
showed a comparatively larger increase in the treatment
group compared with the placebo group, ( p=0.0118).
Conclusions These results confirm tolerability and
safety of this novel biological agent in established diffuse
SSc. The value of a placebo treated control group in
small clinical trials evaluating skin disease in SSc is
confirmed. Potential improvement in mRSS and changes
in PIIINP in cases receiving active therapy suggest that
this intervention may be of clinical benefit and warrants
further evaluation.
INTRODUCTION
To cite: Quillinan NP,
McIntosh D, Vernes J, et al.
Ann Rheum Dis Published
Online First: [ please include
Day Month Year]
doi:10.1136/annrheumdis2013-203674
Systemic sclerosis (SSc) is a multisystem disease that
is associated with inflammation, fibrosis and vasculopathy. It is uncommon but has high morbidity
and the highest case-specific mortality of any
rheumatic disorder with 50% of patients dying or
developing major internal organ complications
within 3 years of diagnosis.1 There are two major
subsets of systemic sclerosis, limited cutaneous SSc
and diffuse cutaneous SSc (dcSSc).2
Although there is understandable focus on the
high burden of severe skin and internal organ
involvement in early stage diffuse SSc, with less
than 3 years disease duration,1 there is also
substantial burden at later stages and this has been
highlighted in recent cohort studies.3
Traditional models of pathogenesis have suggested that early vascular events associated with
autoimmunity and inflammation lead to subsequent
fibrosis. Although this is plausible and supported by
preclinical mechanistic studies it is clear that a
broad range of biological processes interact in SSc
and that these include involvement of key profibrotic cytokines such as transforming growth factor-β
and connective tissue growth factor as well as
proinflammatory cytokines such as interleukin 6
(IL-6) and tumour necrosis factor (TNF)α. There is
also increasing evidence of an imbalance in Th1/
Th2/Th17/Treg system promoting inflammation
and fibrosis and activation of B cells promoting
production of autoantibodies.4 Diffuse SSc is often
categorised as early-stage or established/late-stage
disease and it is possible that the pathogenic factors
underlying the distinct phases of the disease are different. In particular, pathogenic drivers of late-stage
disease are less clear, but there is emerging evidence
that persistent perturbation of immune cell function may be relevant.5 The cornerstone of management of early stage diffuse SSc is broad spectrum
immunosuppression.6 Emerging data support the
benefit of immunosuppression for skin and lung
fibrosis in SSc, especially when given at the early
stages of disease.7
Study drug
Hyperimmune
caprine
serum
(AIMSPRO,
Anti-inflammatory IMmuno -Suppressive PROduct)
is a goat serum extract derivative supplied frozen
and thawed to a liquid for immediate injection. It is
produced in goats raised and housed at a licensed
facility in Tasmania, Australia. The animals are vaccinated using detergent-inactivated HIV viral lysate.
Serum is shipped frozen to the manufacturing facility in Victoria, Australia where the sera are pooled,
fractionated and diafiltered to preserve various
macromolecules, immunoglobulin species and low
molecular weight components prior to further processing nanofiltration and vialing.
The final product contains principally caprine
immunoglobulins but also various small molecular
weight species including cytokines. ELISA characterisation of the serum has revealed the presence of
a range of components including the cytokines IL-4
and IL-10, proopiomelanocortin, arginine vasopressin, β-endorphin and corticotropin-releasing factor.
Previous studies have shown that when peripheral
Quillinan Article author (or their employer) 2013. Produced
1
CopyrightNP, et al. Ann Rheum Dis 2013;0:1–6. doi:10.1136/annrheumdis-2013-203674 by BMJ Publishing Group Ltd (& EULAR) under licence.
2. Clinical and epidemiological research
blood mononuclear cells are isolated and incubated with serial
dilutions of AIMSPRO, raw hyperimmune serum and
heat-inactivated sera induced the release of IL-10 in vitro.
Studies in patients with multiple sclerosis and chronic inflammatory demyelinating polyneuropathy have shown a sodium
channel opening effect, which is thought to be one of several
potential mechanisms of action of this novel medication.8
PATIENTS AND METHODS
Study design
The primary objective of this double blind, placebo controlled
parallel group study was to assess safety and feasibility of using
this novel agent in late-stage dcSSc. The secondary objectives
were assessment of possible treatment effect (using clinical outcomes such as modified Rodnan Skin Score (mRSS), SSc Health
Assessment Questionnaire Disability Index (SSc HAQ-DI) and
Short Form 36 (SF-36) quality of life questionnaire) and the
exploration of candidate biomarkers (such as von Willebrand
factor (vWF), serum IL-2 receptor (sIL-2R), PIIINP as well as
,
multiplex analysis of serum and plasma). The study was
approved by the local Ethics Committee. At completion of the
blinded phase all subjects were offered 26 weeks of treatment
with AIMSPRO on a compassionate basis and efficacy and
safety end points were evaluated at 52 weeks.
Study patients
This was a single-centre double-blind placebo-controlled study
conducted at the Royal Free London NHS Foundation Trust,
London, UK. Eligible patients were recruited from outpatient
clinics and chart reviews. We treated 10 subjects with established
dcSSc using hyperimmune goat serum and compared outcome
over 6 months with 10 control subjects receiving placebo.
Subjects were randomised to receive 1 ml study drug or placebo
subcutaneously twice weekly for 6 months. The first two doses
of medication were administered in the study centre under
supervision at week 0, day 0 and week 0, day 3. Subjects were
followed for 26 weeks with additional safety visits occurring at
weeks 2, 6, 14, 20 and a final safety visit at week 52 (6 months
after the end of the double-blind phase).
The major inclusion criteria were: fulfilling the 1980
Preliminary classification criteria for systemic sclerosis.9 Clinical
classification of diffuse SSc (LeRoy criteria)2 and at least 3 years
duration since the first non-Raynaud’s manifestation of SSc.
The exclusion criteria were: use of a putative diseasemodifying drug within 1 month of screening, patients receiving
previous administrations of AIMSPRO or those with a history
of known allergy to animal proteins.
Concomitant medication
Throughout the study period, all medications were kept stable
where possible. Medications contraindicated during the treatment phase included other investigational drugs, other biological therapies or immunosuppressive agents. Sodium channel
blocking agents such as anticonvulsant medications were also
contraindicated. Medications that were allowed during treatment phase included prednisolone up to 10 mg/day, and medications for treatment of Raynaud’s phenomenon.
Clinical assessments
For evaluation of skin thickness, the 17-site mRSS was used,
with each site assessed on a scale of 0–3 with a maximum score
of 51. For individual subjects, clinically meaningful change in
skin score was defined as greater than 4 skin score units and at
least 20% change in overall mRSS, as described previously.10
2
The same assessor performed all assessments for the duration of
the trial ensuring a standardised approach. Additional mRSS
data from 26-week unblinded administration were also available
and this provided additional opportunities for exploratory analysis of AIMSPRO in cases that had previously received placebo.
Pulmonary function and echocardiogram were performed using
standard techniques. All subjects completed SSc-HAQ,11 SF-36,
neuropathic pain visual analogue scale (VAS) and UK functional
score 12 (an 11-item 4-grade questionnaire, developed specifically to assess functional capacity in scleroderma patients) questionnaires at baseline visit, week 6 and week 26. In addition,
exploratory clinical assessments such as Medical Research
Council sum score (an assessment of muscle power), sniff nasal
inspiratory pressure (SNIP an assessment of respiratory muscle
,
function) and the R-R interval (a surrogate marker of autonomic
dysfunction) were performed at the same time points.
Laboratory assessments
Serum and plasma samples were taken at each visit to assess
safety and for exploratory biomarker analyses. These followed
the template of recent expert consensus regarding exploratory
biomarker studies in SSc trials.13 Serum amino terminal propeptide of type III collagen (PIIINP), a marker of fibrosis, sIL-2R, a
marker of inflammation, vWF, a marker of vasculopathy were
analysed using standard ELISA assays by Quest Diagnostics,
California, USA, under good clinical practice (GCP) conditions.
Adverse events
All subjects were monitored for adverse events (AEs) occurring
after screening, even if they had not received study drug.
Serious AEs (SAEs) occurring after the first injection and up to
6 months after the last injection were documented and reported
to the sponsor and medical monitor within 24 h.
Statistical analysis
Key measures of efficacy in this study were change in SSc
HAQ-DI from baseline to week 26, change in mRSS from baseline to week 26, the change in the UK Function Score from
baseline to week 26 and the change in SF-36 scales from baseline to week 26. Change in HAQ-DI and mRSS analysed as a
continuous variable were the prespecified efficacy end points.
Responder frequency analysis for mRSS was also included post
hoc to capture clinically meaningful change in mRSS.
Inferential testing has been performed to compare groups in
the change from baseline to each post-treatment visit. Two-sided
p values <0.05 were considered statistically significant. A mixed
models repeated measures analysis was performed on the data
in the first instance. In some cases the mixed models algorithm
could not converge, so a standard repeated measures analysis of
variance was performed. The use of the mixed models repeated
measures also allowed the calculation of probabilities for the
adjusted mean change value tested against a standard value of
zero, whereas the repeated measures analysis of variance does
not calculate those probabilities, but does provide the 95% CI.
Other analyses included a responder frequency analysis to
capture individual patient data within the more variable cohort
changes in mean mRSS. The unconditional z-pooled test was
used to analyse responder frequency analysis, as recommended
by Lydersen et al14.
RESULTS
Study cohort
Twenty-two subjects were screened and there were two screen
failures. Twenty subjects were enrolled into the study, all of
Quillinan NP, et al. Ann Rheum Dis 2013;0:1–6. doi:10.1136/annrheumdis-2013-203674
3. Clinical and epidemiological research
Table 1 Demographics of the study cohort
Characteristic
AIMSPRO
Age (years)
n
Mean (SD)
Min, Max
Median
n
Mean (SD)
Min, Max
Median
n
Mean (SD)
Min, Max
Median
n
Mean (SD)
Min, Max
Median
n (%)
n (%)
n (%)
n (%)
n (%)
n (%)
n (%)
n (%)
n
Mean (SD)
Min, Max
Median
n
Mean (SD)
Min, Max
Median
Weight (Kg)
Height (m)
Body mass index (kg/m2)
Gender
Male
Female
Caucasian
Asian
Other
Non-smoker
Ex-smoker
Current smoker
Current smoker
Race
Smoking status
Number of pack years
Ex-smoker
whom received at least one dose of study medication. Of these,
17 completed the study and there were 3 withdrawals. None
were lost to follow-up. Demographic characteristics of the
cohort are summarised in table 1 and disease characteristics at
baseline are outlined in table 2. These features were as expected
for a cohort of subjects with established diffuse SSc.
Table 2 Baseline characteristics of the study cohort
Parameter
Disease duration, years
Mean (SD)
Median
Min, Max
mRSS
Mean (SD)
Median
Min, Max
Autoantibodies, no. (%)
Antitopoisomerase
RNA Polymerase III
Other
Placebo (n=10)
AIMSPRO (n=10)
10.95 (5.5)
10.9
3.7, 20
10.21 (8.5)
7.99
3, 33
13.2 (4.7)
12.5
7, 22
16.9 (9.1)
12.0
6, 31
4 (40)
3 (30)
3 (30)
2 (20)
5 (50)
3 (30)
mRSS, modified Rodnan Skin Score.
Quillinan NP, et al. Ann Rheum Dis 2013;0:1–6. doi:10.1136/annrheumdis-2013-203674
Placebo
10
53.3 (12.66)
35, 75
55.7
10
75.80 (20.531)
51, 123
75.50
10
1.64 (0.089)
1.5, 1.8
1.64
10
27.93 (5.484)
21.8, 36.7
27.66
1 (10.0)
9 (90.0)
8 (80.0)
2 (20.0)
0 (0.0)
5 (50.0)
4 (40.0)
1 (10.0)
1
5.3
5, 5
5.3
3
14.0 (15.39)
1, 31
10.0
10
53.6 (13.23)
29, 77
57.2
10
70.00 (14.765)
52, 98
70.00
10
1.63 (0.083)
1.5, 1.8
1.62
10
26.47 (4.976)
20.1, 32.5
26.75
1 (10.0)
9 (90.0)
9 (90.0)
0 (0.0)
1 (10.0)
7 (70.0)
3 (30.0)
0 (0.0)
0 (0.0)
0 (0.0)
0 (0.0)
0 (0.0)
3
14.5 (20.02)
1, 38
5.0
Safety and adverse events
All subjects in both groups had at least one AE and AEs were
frequent in both groups in keeping with the high morbidity of
the disease. There were numerically more AEs in the placebo
group compared with the treatment group (though it did not
reach statistical significance), 154 in the placebo group and 139
in the treatment group. This supports a conclusion that the
study drug was safe and well tolerated, although a larger study
would be needed to explore if AEs are significantly less than for
placebo. Details of AEs are provided in table 3.
The most commonly reported AEs were injection site reactions, cutaneous or musculoskeletal-related issues (such as skin
itching, joint pains and ischaemic digital ulcers) and infections.
Table 3
Summary of adverse events (AEs)
Parameter
Placebo
AIMSPRO
Total number of AEs
Possibly/probably related to study medication
Number of patients reporting grade 3/4 AEs (severe)
Number of mild AEs
Number of moderate AEs
Number of severe AEs
154
18
5
59
84
11
139
12
4
59
76
4
3
4. Clinical and epidemiological research
Table 4 Summary of serious adverse events (SAEs)
Parameter
Placebo
AIMSPRO
Number of subjects reporting
SAEs
Total number of SAEs
Withdrawal due to AEs and
SAEs
SAE by organ system
3
3
6
2
4
1
Intestinal obstruction
×2
Panenteric dysmotility
Viral meningitis
Pyelonephritis
Cerebral infarct
Pulmonary embolus
Atrial fibrillation
Respiratory tract
infection
Ischaemic digital ulcer
Transient injection site reactions occurred in both groups, but
were more common in the treatment group. The frequency of
other AEs was similar in both groups. There were no statistically
significant differences in the safety laboratory values throughout
the study and no differences were noted between the groups in
vital signs, physical examination, electrocardiography or
echocardiography.
There were six SAEs in three patients in the placebo group
and four SAEs in three patients in the treatment group. Two
patients in the placebo group and one in the treatment group
withdrew due to AEs or SAEs. There were no deaths during the
course of the study. Details of SAEs are provided in table 4.
analysis for the primary data shows mean mRSS fell by 1.4±4.7
units with active treatment but worsened by 2.1±6.4 units on
placebo ( p=0.181, unpaired t test) when baseline values were
compared with 26 weeks.
Because some cases demonstrated clinically meaningful
improvement in mRSS, we proceeded to post hoc analysis of
responder frequency in active and placebo treated subjects. In
the active treatment group one (10%) patient had at least 20%
improvement from baseline in mRSS at week 6, and the
number had increased to five (50.0%) at week 26. In contrast
the placebo group had a greater proportion of patients (four
patients; 40.0%) with response at week 6, and fewer patients
(one patient; 10.0%) at week 26. The difference between
groups at week 26 showed a strong trend towards statistical significance ( p=0.062) by the unconditional z-pooled test,
figure 1A. To extend these skin score data we undertook
further analysis from an extended dataset, for patients receiving
AIMSPRO on a compassionate basis for 26 weeks after completion of the double-blind phase of the study. These data are generally supportive of the trend for improvement seen in the
blinded phase. Thus, skin score data were available for seven
additional cases treated for 26 weeks with AIMSPRO, and
from three cases that chose not to take the drug but that were
observed for a further 26 weeks off treatment. For this larger
patient group the change in MRSS between baseline and
26 weeks was −2.00±1.03 for those treated with AIMSPRO
(n=17) and +2.39±1.64 in those not receiving active therapy
(n=13). Using Student t test and corresponding CIs, this difference reached statistical significance ( p=0.025), although the
limitations of open label data and a post hoc analysis must be
considered.
Changes in skin score
We first analysed the difference from baseline score to 26 weeks
as an outcome variable, and the difference between the groups
using Student t test and corresponding CIs. Using this approach,
Other outcomes
Mean±SD for HAQ-DI at baseline was 1.2±0.07 for the active
group and 1.6±0.63 for the placebo group and at 26 weeks it
Figure 1 Improvement in modified Rodnan Skin Score (mRSS) and in neuropathic pain visual analogue scale (VAS) from baseline to week 26 in
active treatment arm. (A) There was an increase in mean mRSS in the placebo treated subjects and improvement in those receiving active therapy.
This did not reach statistical significance but changes were driven by the larger number of cases on active treatment that showed clinically
meaningful improvement in mRSS during the trial (>4 skin score units and 20% of baseline mRSS). The lines marked in bold show cases with
significant improvement on active treatment or placebo. Responder frequency analysis showed a strong trend in favour of active treatment
( p=0.062). (B) Neuropathic pain VAS showed a significant difference between groups at week 26 with an improvement in the treatment group and
no significant change in the placebo group.
4
Quillinan NP, et al. Ann Rheum Dis 2013;0:1–6. doi:10.1136/annrheumdis-2013-203674
5. Clinical and epidemiological research
In the eight domains of SF-36, the only domain to show
some change was Role Physical, which showed a worsening in
the placebo group and maintenance or stabilisation in the treatment group between baseline and week 26, with a trend to significance between the groups ( p=0.07). The Medical Research
Council sum score, SNIP and R–R interval did not show any
statistically significant differences between the treatment groups.
Neuropathic pain VAS showed a significant difference between
groups at week 26 with an improvement in the treatment group
and no change in the placebo group, p=0.0461, figure 1B.
Lung function indices showed a trend of benefit for active
treatment compared with the placebo group for those variables
that reflect respiratory effort (forced vital capacity and forced
expiratory volume in one second). However when background
disease was taken into account, there was no significant difference between the two treatment groups. Carbon monoxide
transfer factor and total lung capacity did not change during the
study.
Serum biomarkers
There were no statistically significant changes in vWF and
sIL-2R between the two groups comparing baseline to week 26.
However, PIIINP (mg/L) showed a comparatively larger increase
in the treatment group compared with the placebo group; treatment group baseline 6.9±3.8, 26 weeks 15.4±10.1, placebo
group baseline 5.3±2.4, 26 weeks 5.6±2.7, p=0.0118, figure 2.
DISCUSSION
Figure 2 Graphical representation of change from baseline to week
26 for candidate serum biomarkers. There were no statistically
significant changes in von Willebrand factor (vWF; A) or sIL-2R
( panel B) between baseline and end of study. However, PIIINP (C)
showed significant increase in the active treatment arm compared
with the placebo group. This novel observation may reflect increased
connective tissue remodelling in late-stage SSc cases receiving active
treatment as it occurs in the context of improvement in average skin
score.
was 1.2±0.98 for the active group and 1.6±0.55 for the
placebo group( p=0.47). There was no statistically significant
difference between the groups for any of the other parameters
of SSc HAQ (including patient global VAS), physician global
VAS ( p=0.35) or the UK functional score ( p=0.52).
Quillinan NP, et al. Ann Rheum Dis 2013;0:1–6. doi:10.1136/annrheumdis-2013-203674
The primary objective of this study was to show safety and feasibility of using this novel agent in dcSSc, a complex multisystem
autoimmune disease. The results of our study confirm that this
drug is safe and well tolerated. AEs were frequent and there
were more AEs and SAEs in the placebo group, but this was not
statistically significant. None of the SAEs was deemed to be due
to the study medication. Late stage disease was chosen because
it provides a robust platform for safety analysis through the
likely burden of disease and facilitated a placebo-controlled
study design. The secondary objective was to explore potential
efficacy. For skin score, a responder analysis showed significant
benefit in the active treatment arm and a trend for improvement
in mean skin score during the blinded phase of the study, and
significant improvement when those receiving open label active
therapy after the placebo controlled phase, analysed after
unblinding. These data are reminiscent of improvement in skin
score that was also observed in the treatment group in patients
with late stage dcSSc in the oral collagen trial,5 another novel
immunomodulatory therapy.
The biological basis for any treatment effect from AIMSPRO
is unclear at this stage and may be complex. However, data
suggest a direct immunomodulatory effect that includes modulation of serum levels of relevant cytokines such as IL-10 (SH,
personal communication). Previous studies have suggested that
modulation of IL-10 activity may be one of the relevant effects
of this agent and we confirmed that levels of IL-10 were significantly reduced at 6 weeks after starting AIMSPRO compared
with placebo (data not shown). In addition, AIMSPRO contains
immunoglobulins and this may be relevant in that there are
some reports suggesting that immunoglobulins may be of
benefit in cases of SSc.15
Other interesting results include an improvement in neuropathic pain VAS in the treatment group which is in keeping with
the potential sodium channel effect of this treatment. Pain is a
major morbidity in SSc and is consistently ranked highly as a
patient reported outcome and component of the SSc-HAQ.
5
6. Clinical and epidemiological research
The aetiology of pain is multifactorial and related to tissue
ischaemia, musculoskeletal inflammation and possibly intermittent release of neuropathic mediators: hence the benefit
observed with AIMSPRO is interesting and potentially clinically important.
Though there was substantial functional disability in the
patients as evidenced by HAQ-DI baseline scores, there was no
significant change in HAQ-DI between groups from baseline to
26 weeks, which was similar to other trials.5 16 17 A recent
meta-analysis of seven scleroderma trials did show a downward
trend in HAQ-DI at 6 months but the change in HAQ-DI was
not significant at 12 months. However, all of the trials in this
meta-analysis were conducted in patients with early disease.18
The study cohort was not recruited to evaluate lung function
and although the changes are interesting, they need to be interpreted with caution. Lung function data show a trend to
improvement in forced vital capacity and forced expiratory
volume in one second in the active arm. The main changes are
in components of the lung function that involve patient effort,
raising the possibility that muscle weakness may be one explanation, though the SNIP data and total lung capacity did not
show any changes between groups. Two subjects in the placebo
group deteriorated compared with one in the treatment group.
All three had pre-existing lung disease and one of the subjects in
the placebo group had to be withdrawn due to worsening
lung disease necessitating further immunosuppression. The
Quinapril in Scleroderma (QUINS) trial also showed a trend
to improvement in the active treatment group, the mechanism
of this was unknown but was not due to differences in
immunosuppression.19
Biomarker analysis shows no changes for vWF or sIL-2R, but
there are interesting changes in PIIINP with a significant
increase at 26 weeks in the treatment group compared with the
control group. The mechanism for this is unknown as PIIINP
has previously been proposed as a marker of fibrotic burden and
is used to monitor potential hepatic fibrosis in cases of psoriasis
treated with methotrexate. It is noteworthy that baseline values
in both groups are in keeping with other studies.16 17 20
However, the CAT-192 trial showed no change between groups
at end of study and the Infliximab trial showed a decrease in
values from baseline to end of study. The conflicting results may
be explained by the differences in disease duration of subjects in
these two studies and our study subjects. These data warrant
further prospective evaluation in a larger cohort.
Strengths of this study are a double-blind design with a
placebo group enabling safety assessment and comparison
between groups for efficacy signals; the same assessor for mRSS
throughout the trial, and a relatively uniform patient group in
late-stage disease, reducing the natural variability between presentations seen in early inflammatory disease. Our study has
advantages over an open label design used elsewhere that makes
a potential efficacy signal difficult to detect.16 21
In conclusion, we report a pilot study confirming safety and
tolerability of AIMSPRO in established dcSSc. Our data also
suggest possible efficacy based upon improvement in mRSS in
some patients, beneficial effect on pain and trend for improvement in other variables. Changes in serum PIIINP are of interest
and warrant further investigation and replication in a larger
cohort.
Funding This study was sponsored and funded by Daval International.
Competing interests SH, DM are employees of Daval International. JV is
consultant to Daval International. CPD, NPQ have no competing interests.
Ethics approval Royal Free Hospital and Medical School Research Ethics
Committee.
Provenance and peer review Not commissioned; externally peer reviewed.
REFERENCES
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5
6
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9
10
11
12
13
14
15
16
17
18
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20
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Contributors All authors were involved in data acquisition, design, analysis and
writing of the manuscript and approved the final submitted version.
6
Shand L, Lunt M, Nihtyanova S, et al. Relationship between change in skin score
and disease outcome in diffuse cutaneous systemic sclerosis: application of a latent
linear trajectory model. Arthritis Rheum 2007;56:2422–31.
LeRoy EC, Black C, Fleischmajer R, et al. Scleroderma (systemic sclerosis):
classification, subsets and pathogenesis. J Rheumatol 1988;15:202–5.
Nihtyanova SI, Tang EC, Coghlan JG, et al. Improved survival in systemic sclerosis is
associated with better ascertainment of internal organ disease: a retrospective
cohort study. QJM 2010;103:109–15.
Denton CP. Therapeutic targets in systemic sclerosis. Arthritis Res Ther 2007;9(Suppl
2):S6.
Postlethwaite AE, Wong WK, Clements P, et al. A multicenter, randomized,
double-blind, placebo-controlled trial of oral type I collagen treatment in patients
with diffuse cutaneous systemic sclerosis: I. oral type I collagen does not improve
skin in all patients, but may improve skin in late-phase disease. Arthritis Rheum
2008;58:1810–22.
Quillinan NP, Denton CP. Disease modifying treatment in Systemic Sclerosis: current
status. Curr Opin Rheumatol 2009;21:636–41.
Kowal-Bielecka O, Landewe R, Avouac J, et al. EULAR recommendations for the
treatment of systemic sclerosis: a report from the EULAR Scleroderma Trials and
Research group (EUSTAR). Ann Rheum Dis 2009;68:620–8.
Moore CEG, Hannan R, McIntosh D. In vivo, human peripheral nerve strength
duration time constant changes with Aimspro implicate altered sodium channel
function as a putative mechanism of action. J Neurol Sci 2005;238:S238.
Subcommittee for Scleroderma Criteria of the American Rheumatism Association
Diagnostic and Therapeutic Criteria Committee. Preliminary criteria for the
classification of systemic sclerosis (scleroderma). Arthritis Rheum 1980;23:581–90.
Khanna D, Furst DE, Hays RD, et al. Minimally important difference in diffuse
systemic sclerosis: results from the D-penicillamine study. Ann Rheum Dis
2006;65:1325–9.
Steen VD, Medsger TA Jr. The value of the Health Assessment Questionnaire and
special patient-generated scales to demonstrate change in systemic sclerosis patients
over time. Arthritis Rheum 1997;40:1984–91.
Serednicka K, Smyth AE, Black CM, et al. Using a self-reported functional score to
assess disease progression in systemic sclerosis. Rheumatology 2007;46:1107–10.
Chung L, Denton CP, Distler O, et al. Clinical trial design in scleroderma. Clin Exp
Rheumatol 2012;30:S97–102.
Lydersen S, Langaas M, Bakke Ø. The exact unconditional z-pooled test for equality
of two binomial probabilities: optimal choice of the berger and Boos Confidence
coefficient. J Stat Comput Simulation 82:1311–16.
Levy Y, Amital H, Langevitz P, et al. Intravenous immunoglobulin modulates
cutaneous involvement and reduces skin fibrosis in systemic sclerosis: an open-label
study. Arthritis Rheum 2004;50:1005–7.
Denton CP, Engelhart M, Tvede N, et al. An open-label pilot study of infliximab
therapy in diffuse cutaneous systemic sclerosis. Ann Rheum Dis 2009;68:1433–9.
Denton CP, Merkel PA, Furst DE, et al. Recombinant human anti-transforming
growth factor beta1 antibody therapy in systemic sclerosis: a multicenter,
randomized, placebo-controlled phase I/II trial of CAT-192. Arthritis Rheum
2007;56:323–33.
Merkel PA, Silliman NP, Clements PJ, et al. Patterns and predictors of change in
outcome measures in clinical trials in scleroderma: an individual patient
meta-analysis of 629 subjects with diffuse cutaneous systemic sclerosis. Arthritis
Rheum 2012;64:3420–9.
Gliddon AE, Doré CJ, Black CM, et al. Prevention of vascular damage in
scleroderma and autoimmune Raynaud’s phenomenon: a multicenter, randomized,
double-blind, placebo-controlled trial of the angiotensin-converting enzyme inhibitor
quinapril. Arthritis Rheum 2007;56:3837–46.
Lee YJ, Shin KC, Kang SW, et al. Type III procollagen N-terminal propeptide, soluble
interleukin-2 receptor, and von Willebrand factor in systemic sclerosis. Clin Exp
Rheumatol 2001;19:69–74.
Khanna D, Saggar R, Mayes MD,, et al A one-year, phase I/IIa, open-label pilot trial
of imatinib mesylate in the treatment of systemic sclerosis-associated active
interstitial lung disease. Arthritis Rheum 2011;63:3540–6.
Quillinan NP, et al. Ann Rheum Dis 2013;0:1–6. doi:10.1136/annrheumdis-2013-203674