This document discusses recombinant DNA technology and its applications. It begins by defining recombinant DNA technology as joining DNA molecules from different species and inserting them into a host organism. It then outlines key applications in medicine, forensic science, agriculture, and food industry. Specifically, it provides examples of using recombinant DNA technology to produce insulin, vaccines, interferon, and antibiotics for medicine. It also discusses uses in identifying criminals through DNA fingerprinting in forensic science and creating genetically modified crops with increased yields in agriculture.

1. RECOMBINANT DNA TECHNOLOGY 1

2. RECOMBINANT DNA TECHNOLOGY
2
APPLICATIONS OF RECOMBINANT D
NA TECHNOLOGY
BABAR KHAN
UOS,SWAT
MICROBIOLOGY

3. ☆ RECOMBINANT DNA TECHNOLOGY
 Recombinant DNA technology, joining
together of DNA molecules from two
different species that are inserted into
a host organism to produce new genetic
combinations that are of value to science,
medicine, agriculture, and industry.
RECOMBINANT DNA TECHNOLOGY 3

4. ☆ APPLICATIONS
 Application in medicine
 Application in forensic science
 Application in agriculture
 Application in food industry
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5. ☆ APPLICATION IN MEDICINE
 Recombinant DNA technology had made it possible
to treat different disease by inserting new genes in
place of mutated gene in human body.
 Brought many revolutionary changes in the field of
medicine and introduce such method of treating
diseases and delivering the drug.
 Recombinant DNA technology in medicine also used
in a series of procedures that are used to join
together DNA segments.
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6. • A recombinant DNA molecule is constructed from
of two are more different DNA molecules
• under certain condition recombinant
DNA molecule can enter
A cell and replicate
their either on its own or
after it has been
integrated into
Chromosome
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7. 1. INSULIN
 Insulin is a hormone
 made of protein
 secreted in the pancreas by some cells called as islet of
Langerhans.
 This hormone is responsible for controlling the glucose
level in humans.
 If a person has decreased amount of insulin in his body,
he will suffer from a disease called diabetes.
 Recombinant DNA technology has allowed the scientists
to develop human insulin by using bacteria as a host cell
.
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8. 2. vaccines
 vaccines is a biological substances that is prepared
from the suspension of weak or dead pathogenic cells
 it is injected in the body to enhance the production of
antibodies against a particular antigen
 recombinant DNA technology has made it easier for
scientists to develop vaccine by cloning a gene used for
protective antigen protein
 viral vaccine are mostly develop in this technique
 Example : HBV
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9. 3.INTERFERON
 Interferon is a glycoprotein which has the ability to block
the multiplication or division of viruses in the cell or in the
nearby cells
 Interferon can be used to treat cancer like hairy cell
leukemia
 recombinant DNA technology produced this protein using
a bacteria called E.coli
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10. 4.ANTIBIOTICS
 Chemical substances
 used against bacterial infections
 produced by microorganisms as well as in the laboratory
 having the ability to destroy microbes that cause harmful
infections in the body
 Alexander Fleming discovered penicillin in 1928 using
recombinant DNA technology
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11. ☆ APPLICATION IN FORENSIC SCIENCE
 It helps the human being in investigation system to fight
against crime and sort out civil disputes
 When a person commits a crime for instance murder, the
y will leave behind small amount of blood, saliva semen
hair , nails or anything else at the crime scene or in a vict
im
 in such circumstances DNA fingerprinting could help in i
dentifying them
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12. ☆ APPLICATION IN AGRICULTURE
 Recombinant DNA changes the natural genetic makeup
and the characteristics of an organism by inserting DNA
of another organism
 also known as genetic engineering
 Recombinant DNA technology is widely used in agricultu
re to create genetically modified organisms that produce
genetically modified crops
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13. APPLICATION IN AGRICULTURE
 Recombinant DNA technology increased productivity of c
rops
 Enhance crop protection
 Improvement in food processing
 Improved nutritional value
 Better flavor
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14. ☆APPLICATION IN FOOD INDUSTRY
• The recombinant dna technology plays an important role
in the production of food
• The food processing enzymes from recombinant
microorganisms are used in the production of food such as
cheese
• Enzymes which are traditionally isolated from culturable
microorganism,plant and mammalian tissues are offten not
well adapted to the condition used in modren food
production methods
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15. • Thats why we need recombinant dna technology
Steps in manufacture novel enzymes suitable for
food processing conditions
HOW..??
• we can do it by screening
Microorganisms sampled
from diverse environments
• Do modification of known
enzymes using modern of
protein engineering
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16. Example
• Chymosin
• It also known as rennin single polypeptide chain of 323
amino acids
• it possesses two different types of chymosin which are A
and B
• The main coagulating enzyme found in rennin which is
used extensively in cheese production
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17. ☆Steps
• First ,Gene for chymosin are isolated from cow DNA
• second, the plasmid DNA is cut restriction enzymes
• 3rd,then combine chymosin DNA and plasmid DNA with
aid of ligase
• put the recombinant DNA
Into the bacteria through
the process of
transformation
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18. • Chymosin protein is then express into the bacterial
Cells
• after chymosin protein are isolate and use for making
Cheese.
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19. ssssss
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