The document describes an experimental evaluation of the anti-inflammatory activity of Corchorus capsularies in rats. Seeds of C. capsularies were extracted using hydroalcohol and preliminary phytochemical analysis revealed the presence of glycosides, tannins, flavonoids, and phenols. The extract was tested in an egg albumin-induced paw edema model in rats at doses of 250 mg/kg and 500 mg/kg. Both doses showed significant anti-inflammatory effects compared to the control, with the higher dose exhibiting better activity. The results indicate that C. capsularies has significant anti-inflammatory properties worthy of further molecular-level study.

1. Netaji Subhas Chandra Bose Institute Of
Pharmacy
Tatla,Roypara,Chakdaha,Nadia,WB-741222
CERTIFICATEON TOPIC “PRECLANTI-INFLAMMATORY ACTIVITY
OF CRCHORUS CAPSULARIES.”INICAL EVALUATION OF
NAME– JOSIMUDDIN BISWAS
B. PHARM,7TH SEM
UNIVERSITY ROLL--17401916044

2. Herbal medicine is the use of medicinal plant for preventive and treatment of
diseases.
The use of herbal remedies is more prevalent in patients with chronic
diseases such as cancer, diabetis, asthma, and kidney diseases.
 Inflammation is a common condition, not only causing pain, discomfort but
also retards the life style. NSAID are commonly used in the treatment of
inflammation but having the wide range of side effects including ulcer.
There is always a dearth of a herbal medicine which may exert anti
inflammatory action but without any dangerous side effect.
As Crchorus capsularies has been used in the treatment of inflammation in
folk medicine from the ancient time, but no scientific data is available. Here is
an attempt to establish the anti-inflammatory action of Corchorus capsularies
scientifically

4. Experimental animals:
Albino wistar rat weighing 150 to 200g
NSCBIP animal house was well maintained (22±2ºc),
65%relative humidity, 12 hr light and dark cycle.
They were provided with commercial food and tap
water.
Cleaning and sanitation work was done on alternate
days..
Extracts:
The seed of Corporus capsularies ( Jute) > local area
of chakdaha grinded 50 gms on the soxhilation
with 400ml of 70% hydro-ethanolic mixture.

5. SL.NO Name of the test Procedure observation
1 Alkaloids Drug + Mayer s
reagent
Orange colour
2 Glycoside Anthrone +H2SO4 +
heat
Green colour
3 Tannins Drug +lead acetate
+water
Formation of
white ppt.
4 Saponins Drug + water
+shaking
Formation of
honey comb like
forth
5 Flavonoid Zn-HCL acid reduction
test
Red color
6 Phenol Drug + lead acetate + water Formation of
white ppt.

6.  Albino wister rats weight between 200 and 250 gm
 The animal were grouped into 4 groups, 3 animals each, GPI:
Untreated control, GPII: standard (Diclofenac sodium 500mg/kg),
GPIII: Extract250mg/kg(CCLD) and GPIV: Extract500 mg/kg (CCHD)
 The animals were markd on both the hind paws ( right and left) just
beyond tibio- tarsal junction.The initial paw volume (both right and
left) of each rat was measured by mercury displacement method.
 The animals were received treatment as per their treatment group.
After 30 min 0.1 ml of egg albumin were injected
 The paw volume of both legs measured at 15,30,60, and 120 min
 The percentage difference in the right and left paw volumes of each
animal of control and all other treatment group were measured .

7. Extract Preparation:
12%yeild
Preliminary phytochemical analysis of corchorous
capsularies
Name of the test Hyrdroalcohol
Alkaloids -
Glycoside +
Tannins +
Saponins -
Flavonoids +
phenol +

8. Time Untreated control Standard CCLD CCHD
15 26.01+0.21 18.23+0.72*** 23.90+0.43* 21.02+0.54**
30 34.87+0.12 8.31+0.44*** 19.23+0.65*** 17.68+0.76***
60 52.55+0.42 5.88+0.33*** 13.61+0.87*** 9.32+0.13***
120 47.23+0.33 5.07+0.17*** 12.87+0.65*** 9.42+0.45***
All values are mean±sem, p<0.05*,p<0.01**,p<0.001***,vs control
The extracted was evaluated by using 2 different concentration namely
CCLD and CCHD, both of the doses has shown significant anti
inflammatory action in egg albumin induced paw oedema model,
although CCHD has shown better result than CCLD .

9.  The seeds of chorchorous capsularies were
collected, subjected for hydroalcoholic extract,
phytochemical investigation.
 The extract was pharmacologically evaluated by
using egg albumin induced paw oedema model.
 The result obtained from the models was obvious
that Corchorous capsularies has shown significant
anti-inflammatory activity while compared with
control and standared drug.
 From the above findings it can be concluded that
the same may bring light in the field of inflammation
after a detailed molecular level study.

10. 1. Owoyele, Bamidele V., et al. "Anti-inflammatory and antipyretic properties of Corchorus
olitorius aqueous root extract in Wistar rats." Journal of basic and clinical physiology and
pharmacology 26.4 (2015): 363-368.
2.Owoyele, B. V., Oyewole, A. L., Alimi, M. L., Sanni, S. A., & Oyeleke, S. A. (2015). Anti-
inflammatory and antipyretic properties of Corchorus olitorius aqueous root extract in Wistar
rats. Journal of basic and clinical physiology and pharmacology, 26(4), 363-368.
3.Owoyele, Bamidele V., Aboyeji L. Oyewole, Modupe L. Alimi, Shukurat A. Sanni, and Sabitiu A.
Oyeleke. "Anti-inflammatory and antipyretic properties of Corchorus olitorius aqueous root
extract in Wistar rats." Journal of basic and clinical physiology and pharmacology 26, no. 4
(2015): 363-368.
4.Owoyele, B.V., Oyewole, A.L., Alimi, M.L., Sanni, S.A. and Oyeleke, S.A., 2015. Anti-inflammatory
and antipyretic properties of Corchorus olitorius aqueous root extract in Wistar rats. Journal of
basic and clinical physiology and pharmacology, 26(4), pp.363-368.
5.Owoyele BV, Oyewole AL, Alimi ML, Sanni SA, Oyeleke SA. Anti-inflammatory and antipyretic
properties of Corchorus olitorius aqueous root extract in Wistar rats. Journal of basic and
clinical physiology and pharmacology. 2015 Jul 1;26(4):363-8.