TWIST1 protein (twist-related protein 1) is a transcriptional regulator encoded by the TWIST1 gene in humans. It is a basic helix-loop-helix transcription factor and is known to play a prominent role in cancer metastasis.

1. Cell Line:
HMLE Twist-ER cell line (Mani
et al., Cell, 2008)
MCF7 cell line (ATCC)
Method of validation: Mass Cytometry
Primary Antibody:
STJ31126 TWIST Antibody
(STJ29453)
Secondary Antibody 159
Tb-IgG goat anti-rabbit
Dilution ratio: Used at 4 µg/ml
Result
“The antibody can be used to detect high levels of TWIST1. However, when it comes to detection of small expression
differences, this might be challenging by mass cytometry due to the medium signal intensity at high antibody concen-
trations (4 µg/ml). “ - J. Wagner, University of Zurich
Treatment of materials:
The cells were cultured to 70%-80% plating density. For cell detachment, they were washed with pre-warmed PBS
(Gibco) and 1X TrypLETM (Thermo Fisher) was added for 5 min at 37°C. For cell fixation, 1.6% paraformaldehyde
(Thermo Fisher) was added for 10 minutes at room temperature. Then, the cells were pipetted off the plate and col-
lected in cold full growth medium to quench the paraformaldehyde. The cells were spun down and aliquoted in PBS
(Gibco) prior to storage at -80°C.
Protocol:
Fixed cells were washed once with 1x PBS / 0.05% bovine serum albumin / 2mM EDTA cell staining buffer (CSB) prior to
staining with the antibody (STJ92356, 4 µg/ml) in CSB for 45 min at 22°C. The cells were washed three times with CSB.
Then, the secondary antibody, a goat anti-rabbit 159
Tb-IgG (0.25 µg/ml), was applied in CSB for 45 min at 22°C. The cells
were washed with CSB and ddH20 prior to mass cytometry acquisition.
Antibody Customer Review:
STJ31126 TWIST Antibody (STJ29453)
Antibody Specificity:
Antibody Rating:
Overlay histogram showing MCF7 cells
(orange) and HMLE Twist-ER cells (violet)
stained with STJ31126 and anti-rabbit 159Tb-IgG.
HMLE Twist-ER cells overexpress Twist1 (Mani
et al., Cell 2008.)