SlideShare a Scribd company logo
1 of 1
Download to read offline
Neurotoxicity assay using High Content Screening technology
N. Maubon1*, M. Roudaut1, N. Gueguen1, and J. Bursztyka1
1 HCS Pharma, Biopôle, 6 rue Pierre Joseph Colin, 35000 Rennes
1*nathalie.maubon@hcs-pharma.com
Abstract
As shown by AstraZeneca in nature reviews*, one third of the safety
failures is linked to CNS toxicity during the clinical trials of drugs .
To avoid this attrition, the potential neurotoxicity of any drug going
through the blood brain barrier (BBB) needs to be checked and if possible
at the early stage of the research process for new chemical entites (NCE).
This assay can be performed by cell imaging in HCA/HCS.
Methods
Results
Conclusions & Perspectives
 Neurotoxicity of compounds as rotenone or colchicine show that neurite length decreased in higher extent and/or at lower
concentration compared to death of neuronal cells. For example, neurite length decreased with colchicine at 0.01 µM while neuronal
cell death appeared at 0.1 µM.
 Some other compounds as Methylmercure show that neuronal cells are only impacted, showing high variability in neurite length. At
high concentration of Methylmercure (at 100 µM for example), neurites already exist between neighbored resistant cells with high
activity of mitochondria.
 The last 2 other compounds (lead (PbCl2) and Valinomycine) showed toxicity in the same extent between neuronal cells and neurites.
 Conclusion : Neurotoxicity of compounds can be due to neuronal cell death but also due to loss of communication between the
neuronal cells and depending of neurotoxic compounds, the loss of communication (neurites) can appear at lowest dose than
neuronal cell death. This model of neurotoxicity performed in HCS can be a good model during the early stage of research.
Cell culture: neuronal cells (SH-SY5Y) were routinely maintained in MEM/F12 (v/v) supplemented with 10% serum. Neuronal cells were seeded at 10 000
cells/well in 96-well Corning cellBind plates in MEM/F12 supplemented with differentiation agents. Then, cells were incubated at 37 °C in 5 % CO2 for 3 days
for plating and differentiation.
Treatment assay : Neurotoxicity assay was performed by replaced medium in each well by fresh medium with the different compounds to be tested at
different concentrations. After 48h of incubation, cells were incubated with mitotracker during 30 min, fixed and then stained with b III tubulin and Hoechst.
Image acquisition was performed on Operetta plateform (Perkin Elmer) and the analysis of cell number and neurite length was done through Columbus
software (Perkin Elmer).
To get this poster,
please flash the QR-
code
You can use the
I-NIGMA application
from your store
* Cook D, Brown D, Alexander R, March R, Morgan P, Satterthwaite G, Pangalos MN (2014) Lessons learned from
the fate of AstraZeneca’s drug pipeline: a five-imensional framework. Nat Rev Drug Discov. 13(6):419-31.
CTRL Rotenone Colchicine Lead Me-mercure Valinomycine
Numberofcells
Neuritelength
Merge
With
brightfield
bIII-tubulin
Hoechst
Mitotracker
Hoechst
Merge

More Related Content

What's hot

Presentation on protein Array
Presentation on protein ArrayPresentation on protein Array
Presentation on protein ArrayArindam Sain
 
Newborn genetic screening for high risk deafness associated 2
Newborn genetic screening for high risk deafness associated 2Newborn genetic screening for high risk deafness associated 2
Newborn genetic screening for high risk deafness associated 2Dr. Satyender Kumar
 
Tilling @ sid
Tilling @ sidTilling @ sid
Tilling @ sidsidjena70
 
zandona14nipsA0
zandona14nipsA0zandona14nipsA0
zandona14nipsA0Pia Sen
 
Marco Colonna Webinar July 2014
Marco Colonna Webinar July 2014Marco Colonna Webinar July 2014
Marco Colonna Webinar July 2014Alzforum
 
Work on Sorghum bicolor by in-silico method
Work on Sorghum bicolor by in-silico methodWork on Sorghum bicolor by in-silico method
Work on Sorghum bicolor by in-silico methodAnirudh Chaudhary
 
Plegable biology 2
Plegable biology 2Plegable biology 2
Plegable biology 2verdolaga10
 
Biopharmaceuticals
BiopharmaceuticalsBiopharmaceuticals
BiopharmaceuticalsLarry Baum
 
Tumor Microenvironment-Mediated Construction and Deconstruction of Extracellu...
Tumor Microenvironment-Mediated Construction and Deconstruction of Extracellu...Tumor Microenvironment-Mediated Construction and Deconstruction of Extracellu...
Tumor Microenvironment-Mediated Construction and Deconstruction of Extracellu...S KANDAKUMAR CUDDALORE
 
Assessment of immunomolecular_expression_and_prognostic_role_of_tlr7_among_pa...
Assessment of immunomolecular_expression_and_prognostic_role_of_tlr7_among_pa...Assessment of immunomolecular_expression_and_prognostic_role_of_tlr7_among_pa...
Assessment of immunomolecular_expression_and_prognostic_role_of_tlr7_among_pa...dr.Ihsan alsaimary
 
Microarray and its application
Microarray and its applicationMicroarray and its application
Microarray and its applicationprateek kumar
 
ENHANCED COMPLETE GENOME SEQUENCING OF FOOT-AND-MOUTH DISEASE VIRUS USING PRO...
ENHANCED COMPLETE GENOME SEQUENCING OF FOOT-AND-MOUTH DISEASE VIRUS USING PRO...ENHANCED COMPLETE GENOME SEQUENCING OF FOOT-AND-MOUTH DISEASE VIRUS USING PRO...
ENHANCED COMPLETE GENOME SEQUENCING OF FOOT-AND-MOUTH DISEASE VIRUS USING PRO...EuFMD
 
Christian Haas Webinar July 2014
Christian Haas Webinar July 2014Christian Haas Webinar July 2014
Christian Haas Webinar July 2014Alzforum
 

What's hot (19)

Presentation on protein Array
Presentation on protein ArrayPresentation on protein Array
Presentation on protein Array
 
Newborn genetic screening for high risk deafness associated 2
Newborn genetic screening for high risk deafness associated 2Newborn genetic screening for high risk deafness associated 2
Newborn genetic screening for high risk deafness associated 2
 
Tilling @ sid
Tilling @ sidTilling @ sid
Tilling @ sid
 
zandona14nipsA0
zandona14nipsA0zandona14nipsA0
zandona14nipsA0
 
120229 mol med dag jmv
120229 mol med dag jmv120229 mol med dag jmv
120229 mol med dag jmv
 
Marco Colonna Webinar July 2014
Marco Colonna Webinar July 2014Marco Colonna Webinar July 2014
Marco Colonna Webinar July 2014
 
Tessa Poster for IP
Tessa Poster for IP Tessa Poster for IP
Tessa Poster for IP
 
The use of tissue array
The use of tissue arrayThe use of tissue array
The use of tissue array
 
Work on Sorghum bicolor by in-silico method
Work on Sorghum bicolor by in-silico methodWork on Sorghum bicolor by in-silico method
Work on Sorghum bicolor by in-silico method
 
Mehraj
Mehraj Mehraj
Mehraj
 
Plegable biology 2
Plegable biology 2Plegable biology 2
Plegable biology 2
 
Biopharmaceuticals
BiopharmaceuticalsBiopharmaceuticals
Biopharmaceuticals
 
Tumor Microenvironment-Mediated Construction and Deconstruction of Extracellu...
Tumor Microenvironment-Mediated Construction and Deconstruction of Extracellu...Tumor Microenvironment-Mediated Construction and Deconstruction of Extracellu...
Tumor Microenvironment-Mediated Construction and Deconstruction of Extracellu...
 
Assessment of immunomolecular_expression_and_prognostic_role_of_tlr7_among_pa...
Assessment of immunomolecular_expression_and_prognostic_role_of_tlr7_among_pa...Assessment of immunomolecular_expression_and_prognostic_role_of_tlr7_among_pa...
Assessment of immunomolecular_expression_and_prognostic_role_of_tlr7_among_pa...
 
Microarray full detail
Microarray full detailMicroarray full detail
Microarray full detail
 
Microarray and its application
Microarray and its applicationMicroarray and its application
Microarray and its application
 
mol med dag 2012 jmv def
mol med dag 2012 jmv defmol med dag 2012 jmv def
mol med dag 2012 jmv def
 
ENHANCED COMPLETE GENOME SEQUENCING OF FOOT-AND-MOUTH DISEASE VIRUS USING PRO...
ENHANCED COMPLETE GENOME SEQUENCING OF FOOT-AND-MOUTH DISEASE VIRUS USING PRO...ENHANCED COMPLETE GENOME SEQUENCING OF FOOT-AND-MOUTH DISEASE VIRUS USING PRO...
ENHANCED COMPLETE GENOME SEQUENCING OF FOOT-AND-MOUTH DISEASE VIRUS USING PRO...
 
Christian Haas Webinar July 2014
Christian Haas Webinar July 2014Christian Haas Webinar July 2014
Christian Haas Webinar July 2014
 

Viewers also liked

CARite Culture & Values
CARite Culture & ValuesCARite Culture & Values
CARite Culture & ValuesCARite
 
[우앙파티] 언남고등학교 - 안전계단
[우앙파티] 언남고등학교 - 안전계단[우앙파티] 언남고등학교 - 안전계단
[우앙파티] 언남고등학교 - 안전계단oeclab
 
In Stadium Fnb Sales Plan
In Stadium Fnb Sales PlanIn Stadium Fnb Sales Plan
In Stadium Fnb Sales PlanTaedy Kang
 
SK플래닛 M&C부문 D-spark #6 Social TV
SK플래닛 M&C부문 D-spark #6 Social TVSK플래닛 M&C부문 D-spark #6 Social TV
SK플래닛 M&C부문 D-spark #6 Social TVkevin lee
 
Exclusive Presentation — 2015 Strategic Diversity Measurement Survey Results
Exclusive Presentation — 2015 Strategic Diversity Measurement Survey ResultsExclusive Presentation — 2015 Strategic Diversity Measurement Survey Results
Exclusive Presentation — 2015 Strategic Diversity Measurement Survey ResultsHuman Capital Media
 
Hands-On Mobile App Testing
Hands-On Mobile App TestingHands-On Mobile App Testing
Hands-On Mobile App TestingDaniel Knott
 
Accessibility: A Journey to Accessible Rich Components
Accessibility: A Journey to Accessible Rich ComponentsAccessibility: A Journey to Accessible Rich Components
Accessibility: A Journey to Accessible Rich ComponentsAchievers Tech
 
Art House Convergence Workshop
Art House Convergence WorkshopArt House Convergence Workshop
Art House Convergence WorkshopBeth Kanter
 
Conversion clickz
Conversion clickzConversion clickz
Conversion clickzAnju Yadav
 
Heal ohio conference-poster
Heal ohio conference-posterHeal ohio conference-poster
Heal ohio conference-posterSudeer K
 
Agile - The Xtreme Labs Way
Agile - The Xtreme Labs WayAgile - The Xtreme Labs Way
Agile - The Xtreme Labs WayAchievers Tech
 

Viewers also liked (17)

Arturo lorandi
Arturo lorandi Arturo lorandi
Arturo lorandi
 
ICSA Guernsey Branch Tax Seminar 2016
ICSA Guernsey Branch Tax Seminar  2016ICSA Guernsey Branch Tax Seminar  2016
ICSA Guernsey Branch Tax Seminar 2016
 
Home made 9 ga
Home made 9 gaHome made 9 ga
Home made 9 ga
 
CARite Culture & Values
CARite Culture & ValuesCARite Culture & Values
CARite Culture & Values
 
poster
posterposter
poster
 
[우앙파티] 언남고등학교 - 안전계단
[우앙파티] 언남고등학교 - 안전계단[우앙파티] 언남고등학교 - 안전계단
[우앙파티] 언남고등학교 - 안전계단
 
In Stadium Fnb Sales Plan
In Stadium Fnb Sales PlanIn Stadium Fnb Sales Plan
In Stadium Fnb Sales Plan
 
SK플래닛 M&C부문 D-spark #6 Social TV
SK플래닛 M&C부문 D-spark #6 Social TVSK플래닛 M&C부문 D-spark #6 Social TV
SK플래닛 M&C부문 D-spark #6 Social TV
 
Exclusive Presentation — 2015 Strategic Diversity Measurement Survey Results
Exclusive Presentation — 2015 Strategic Diversity Measurement Survey ResultsExclusive Presentation — 2015 Strategic Diversity Measurement Survey Results
Exclusive Presentation — 2015 Strategic Diversity Measurement Survey Results
 
Anatomy of a Movement
Anatomy of a Movement Anatomy of a Movement
Anatomy of a Movement
 
Hands-On Mobile App Testing
Hands-On Mobile App TestingHands-On Mobile App Testing
Hands-On Mobile App Testing
 
Accessibility: A Journey to Accessible Rich Components
Accessibility: A Journey to Accessible Rich ComponentsAccessibility: A Journey to Accessible Rich Components
Accessibility: A Journey to Accessible Rich Components
 
Art House Convergence Workshop
Art House Convergence WorkshopArt House Convergence Workshop
Art House Convergence Workshop
 
Conversion clickz
Conversion clickzConversion clickz
Conversion clickz
 
Heal ohio conference-poster
Heal ohio conference-posterHeal ohio conference-poster
Heal ohio conference-poster
 
Icsa Guernsey Fiduciary Update Aug 16
Icsa Guernsey Fiduciary Update Aug 16Icsa Guernsey Fiduciary Update Aug 16
Icsa Guernsey Fiduciary Update Aug 16
 
Agile - The Xtreme Labs Way
Agile - The Xtreme Labs WayAgile - The Xtreme Labs Way
Agile - The Xtreme Labs Way
 

Similar to Neurotoxicity assay using High Content Screening technology

JUNE 2015 POSTER
JUNE 2015 POSTERJUNE 2015 POSTER
JUNE 2015 POSTER5jor13
 
Development of a neuroprotection assay for Parkinson’s disease in vitro model...
Development of a neuroprotection assay for Parkinson’s disease in vitro model...Development of a neuroprotection assay for Parkinson’s disease in vitro model...
Development of a neuroprotection assay for Parkinson’s disease in vitro model...HCS Pharma
 
Genotoxicity assays: assessment of double strand breaks through phospho-H2AX ...
Genotoxicity assays: assessment of double strand breaks through phospho-H2AX ...Genotoxicity assays: assessment of double strand breaks through phospho-H2AX ...
Genotoxicity assays: assessment of double strand breaks through phospho-H2AX ...HCS Pharma
 
Development of cell culture samples for drug screening with bone marrow stem ...
Development of cell culture samples for drug screening with bone marrow stem ...Development of cell culture samples for drug screening with bone marrow stem ...
Development of cell culture samples for drug screening with bone marrow stem ...Apollo Hospitals
 
EricSchmidtUROPposter2014
EricSchmidtUROPposter2014EricSchmidtUROPposter2014
EricSchmidtUROPposter2014Eric Schmidt
 
Endothelial Cell Mediated Delay of Blood Brain Barrier Recovery Following Tra...
Endothelial Cell Mediated Delay of Blood Brain Barrier Recovery Following Tra...Endothelial Cell Mediated Delay of Blood Brain Barrier Recovery Following Tra...
Endothelial Cell Mediated Delay of Blood Brain Barrier Recovery Following Tra...Arthur Stem
 
BIOMED-2014-BT3A.24
BIOMED-2014-BT3A.24BIOMED-2014-BT3A.24
BIOMED-2014-BT3A.24Gen Vigil
 
140514 - HD synaptic characterization
140514 - HD synaptic characterization140514 - HD synaptic characterization
140514 - HD synaptic characterizationAJ Keefe
 
Content Cytotoxicity Studies of Colorectal Carcinoma Cells Using Printed Impe...
Content Cytotoxicity Studies of Colorectal Carcinoma Cells Using Printed Impe...Content Cytotoxicity Studies of Colorectal Carcinoma Cells Using Printed Impe...
Content Cytotoxicity Studies of Colorectal Carcinoma Cells Using Printed Impe...journalBEEI
 
AUTOMATED MULTIVARIATE ANALYSIS OF PHOSPHOLIPIDOSIS IN PRIMARY HEPATOCYTES US...
AUTOMATED MULTIVARIATE ANALYSIS OF PHOSPHOLIPIDOSIS IN PRIMARY HEPATOCYTES US...AUTOMATED MULTIVARIATE ANALYSIS OF PHOSPHOLIPIDOSIS IN PRIMARY HEPATOCYTES US...
AUTOMATED MULTIVARIATE ANALYSIS OF PHOSPHOLIPIDOSIS IN PRIMARY HEPATOCYTES US...Nick Brown
 
SSC4a Report (final)
SSC4a Report (final)SSC4a Report (final)
SSC4a Report (final)Gregory Myles
 
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...Mohamed Khalid Ali Xundhur
 
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...Mohamed Khalid Ali Xundhur
 
Translational Neuroscience Approach in psychiatry..pptx
Translational Neuroscience Approach in psychiatry..pptxTranslational Neuroscience Approach in psychiatry..pptx
Translational Neuroscience Approach in psychiatry..pptxkrishray616
 
Apoptosis of lymphocytes in SLE
Apoptosis of lymphocytes in SLEApoptosis of lymphocytes in SLE
Apoptosis of lymphocytes in SLEAboMuaz
 
Ashwini blotting tech seminar
Ashwini blotting tech seminarAshwini blotting tech seminar
Ashwini blotting tech seminarAshwani Patil
 

Similar to Neurotoxicity assay using High Content Screening technology (20)

JUNE 2015 POSTER
JUNE 2015 POSTERJUNE 2015 POSTER
JUNE 2015 POSTER
 
Development of a neuroprotection assay for Parkinson’s disease in vitro model...
Development of a neuroprotection assay for Parkinson’s disease in vitro model...Development of a neuroprotection assay for Parkinson’s disease in vitro model...
Development of a neuroprotection assay for Parkinson’s disease in vitro model...
 
Genotoxicity assays: assessment of double strand breaks through phospho-H2AX ...
Genotoxicity assays: assessment of double strand breaks through phospho-H2AX ...Genotoxicity assays: assessment of double strand breaks through phospho-H2AX ...
Genotoxicity assays: assessment of double strand breaks through phospho-H2AX ...
 
Development of cell culture samples for drug screening with bone marrow stem ...
Development of cell culture samples for drug screening with bone marrow stem ...Development of cell culture samples for drug screening with bone marrow stem ...
Development of cell culture samples for drug screening with bone marrow stem ...
 
EricSchmidtUROPposter2014
EricSchmidtUROPposter2014EricSchmidtUROPposter2014
EricSchmidtUROPposter2014
 
bioph Abstract
bioph Abstractbioph Abstract
bioph Abstract
 
Endothelial Cell Mediated Delay of Blood Brain Barrier Recovery Following Tra...
Endothelial Cell Mediated Delay of Blood Brain Barrier Recovery Following Tra...Endothelial Cell Mediated Delay of Blood Brain Barrier Recovery Following Tra...
Endothelial Cell Mediated Delay of Blood Brain Barrier Recovery Following Tra...
 
BIOMED-2014-BT3A.24
BIOMED-2014-BT3A.24BIOMED-2014-BT3A.24
BIOMED-2014-BT3A.24
 
synapse microarray ppt
synapse microarray pptsynapse microarray ppt
synapse microarray ppt
 
140514 - HD synaptic characterization
140514 - HD synaptic characterization140514 - HD synaptic characterization
140514 - HD synaptic characterization
 
Content Cytotoxicity Studies of Colorectal Carcinoma Cells Using Printed Impe...
Content Cytotoxicity Studies of Colorectal Carcinoma Cells Using Printed Impe...Content Cytotoxicity Studies of Colorectal Carcinoma Cells Using Printed Impe...
Content Cytotoxicity Studies of Colorectal Carcinoma Cells Using Printed Impe...
 
AUTOMATED MULTIVARIATE ANALYSIS OF PHOSPHOLIPIDOSIS IN PRIMARY HEPATOCYTES US...
AUTOMATED MULTIVARIATE ANALYSIS OF PHOSPHOLIPIDOSIS IN PRIMARY HEPATOCYTES US...AUTOMATED MULTIVARIATE ANALYSIS OF PHOSPHOLIPIDOSIS IN PRIMARY HEPATOCYTES US...
AUTOMATED MULTIVARIATE ANALYSIS OF PHOSPHOLIPIDOSIS IN PRIMARY HEPATOCYTES US...
 
SSC4a Report (final)
SSC4a Report (final)SSC4a Report (final)
SSC4a Report (final)
 
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
 
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
Gene expression profiling in apoptotic mcf 7 cells infected with newcastle di...
 
Translational Neuroscience Approach in psychiatry..pptx
Translational Neuroscience Approach in psychiatry..pptxTranslational Neuroscience Approach in psychiatry..pptx
Translational Neuroscience Approach in psychiatry..pptx
 
Hla typing
Hla typingHla typing
Hla typing
 
Uro instruments- nanotechnology
Uro instruments- nanotechnologyUro instruments- nanotechnology
Uro instruments- nanotechnology
 
Apoptosis of lymphocytes in SLE
Apoptosis of lymphocytes in SLEApoptosis of lymphocytes in SLE
Apoptosis of lymphocytes in SLE
 
Ashwini blotting tech seminar
Ashwini blotting tech seminarAshwini blotting tech seminar
Ashwini blotting tech seminar
 

More from HCS Pharma

Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...
Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...
Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...HCS Pharma
 
Poster : Development of personalized therapeutic targeting in lung cancer wit...
Poster : Development of personalized therapeutic targeting in lung cancer wit...Poster : Development of personalized therapeutic targeting in lung cancer wit...
Poster : Development of personalized therapeutic targeting in lung cancer wit...HCS Pharma
 
Poster - Including the matricial tumoral microenvironment in 3D in vitro mode...
Poster - Including the matricial tumoral microenvironment in 3D in vitro mode...Poster - Including the matricial tumoral microenvironment in 3D in vitro mode...
Poster - Including the matricial tumoral microenvironment in 3D in vitro mode...HCS Pharma
 
HCS PHARMA - (ECM 2022) Development of innovative hiPSC-based model including...
HCS PHARMA - (ECM 2022) Development of innovative hiPSC-based model including...HCS PHARMA - (ECM 2022) Development of innovative hiPSC-based model including...
HCS PHARMA - (ECM 2022) Development of innovative hiPSC-based model including...HCS Pharma
 
HCSPHARMA Importance of microenvironment in cerebral in vitro models for phen...
HCSPHARMA Importance of microenvironment in cerebral in vitro models for phen...HCSPHARMA Importance of microenvironment in cerebral in vitro models for phen...
HCSPHARMA Importance of microenvironment in cerebral in vitro models for phen...HCS Pharma
 
Poster - A single procedure to generate functional hiPSCs-derived liver organ...
Poster - A single procedure to generate functional hiPSCs-derived liver organ...Poster - A single procedure to generate functional hiPSCs-derived liver organ...
Poster - A single procedure to generate functional hiPSCs-derived liver organ...HCS Pharma
 
Poster - BIOMIMESYS® 3D hydroscaffold a matricial microenvironment for physio...
Poster - BIOMIMESYS® 3D hydroscaffold a matricial microenvironment for physio...Poster - BIOMIMESYS® 3D hydroscaffold a matricial microenvironment for physio...
Poster - BIOMIMESYS® 3D hydroscaffold a matricial microenvironment for physio...HCS Pharma
 
BIOMIMESYS® Liver
BIOMIMESYS® LiverBIOMIMESYS® Liver
BIOMIMESYS® LiverHCS Pharma
 
BIOMIMESYS® Adipose tissue
BIOMIMESYS® Adipose tissueBIOMIMESYS® Adipose tissue
BIOMIMESYS® Adipose tissueHCS Pharma
 
BIOMIMESYS® Oncology
BIOMIMESYS® OncologyBIOMIMESYS® Oncology
BIOMIMESYS® OncologyHCS Pharma
 
Importance of matricial and cellular microenvironments in in vitro models for...
Importance of matricial and cellular microenvironments in in vitro models for...Importance of matricial and cellular microenvironments in in vitro models for...
Importance of matricial and cellular microenvironments in in vitro models for...HCS Pharma
 
Simplifying 3d cell culture generation for high content screening with BIOMIM...
Simplifying 3d cell culture generation for high content screening with BIOMIM...Simplifying 3d cell culture generation for high content screening with BIOMIM...
Simplifying 3d cell culture generation for high content screening with BIOMIM...HCS Pharma
 
BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...
BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...
BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...HCS Pharma
 
Abstract : a single procedure to generate functional hi ps cs-derived liver o...
Abstract : a single procedure to generate functional hi ps cs-derived liver o...Abstract : a single procedure to generate functional hi ps cs-derived liver o...
Abstract : a single procedure to generate functional hi ps cs-derived liver o...HCS Pharma
 
Poster – Development and automation of 3D innovative hiPSC-based liver organo...
Poster – Development and automation of 3D innovative hiPSC-based liver organo...Poster – Development and automation of 3D innovative hiPSC-based liver organo...
Poster – Development and automation of 3D innovative hiPSC-based liver organo...HCS Pharma
 
Poster – Development and automation of 3D innovative hiPSC-based liver organo...
Poster – Development	and	automation of 3D innovative	hiPSC-based liver organo...Poster – Development	and	automation of 3D innovative	hiPSC-based liver organo...
Poster – Development and automation of 3D innovative hiPSC-based liver organo...HCS Pharma
 
Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...
Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...
Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...HCS Pharma
 
HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...
HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...
HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...HCS Pharma
 
HCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® Brain
HCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® BrainHCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® Brain
HCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® BrainHCS Pharma
 
A groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffold
A groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffoldA groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffold
A groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffoldHCS Pharma
 

More from HCS Pharma (20)

Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...
Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...
Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...
 
Poster : Development of personalized therapeutic targeting in lung cancer wit...
Poster : Development of personalized therapeutic targeting in lung cancer wit...Poster : Development of personalized therapeutic targeting in lung cancer wit...
Poster : Development of personalized therapeutic targeting in lung cancer wit...
 
Poster - Including the matricial tumoral microenvironment in 3D in vitro mode...
Poster - Including the matricial tumoral microenvironment in 3D in vitro mode...Poster - Including the matricial tumoral microenvironment in 3D in vitro mode...
Poster - Including the matricial tumoral microenvironment in 3D in vitro mode...
 
HCS PHARMA - (ECM 2022) Development of innovative hiPSC-based model including...
HCS PHARMA - (ECM 2022) Development of innovative hiPSC-based model including...HCS PHARMA - (ECM 2022) Development of innovative hiPSC-based model including...
HCS PHARMA - (ECM 2022) Development of innovative hiPSC-based model including...
 
HCSPHARMA Importance of microenvironment in cerebral in vitro models for phen...
HCSPHARMA Importance of microenvironment in cerebral in vitro models for phen...HCSPHARMA Importance of microenvironment in cerebral in vitro models for phen...
HCSPHARMA Importance of microenvironment in cerebral in vitro models for phen...
 
Poster - A single procedure to generate functional hiPSCs-derived liver organ...
Poster - A single procedure to generate functional hiPSCs-derived liver organ...Poster - A single procedure to generate functional hiPSCs-derived liver organ...
Poster - A single procedure to generate functional hiPSCs-derived liver organ...
 
Poster - BIOMIMESYS® 3D hydroscaffold a matricial microenvironment for physio...
Poster - BIOMIMESYS® 3D hydroscaffold a matricial microenvironment for physio...Poster - BIOMIMESYS® 3D hydroscaffold a matricial microenvironment for physio...
Poster - BIOMIMESYS® 3D hydroscaffold a matricial microenvironment for physio...
 
BIOMIMESYS® Liver
BIOMIMESYS® LiverBIOMIMESYS® Liver
BIOMIMESYS® Liver
 
BIOMIMESYS® Adipose tissue
BIOMIMESYS® Adipose tissueBIOMIMESYS® Adipose tissue
BIOMIMESYS® Adipose tissue
 
BIOMIMESYS® Oncology
BIOMIMESYS® OncologyBIOMIMESYS® Oncology
BIOMIMESYS® Oncology
 
Importance of matricial and cellular microenvironments in in vitro models for...
Importance of matricial and cellular microenvironments in in vitro models for...Importance of matricial and cellular microenvironments in in vitro models for...
Importance of matricial and cellular microenvironments in in vitro models for...
 
Simplifying 3d cell culture generation for high content screening with BIOMIM...
Simplifying 3d cell culture generation for high content screening with BIOMIM...Simplifying 3d cell culture generation for high content screening with BIOMIM...
Simplifying 3d cell culture generation for high content screening with BIOMIM...
 
BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...
BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...
BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...
 
Abstract : a single procedure to generate functional hi ps cs-derived liver o...
Abstract : a single procedure to generate functional hi ps cs-derived liver o...Abstract : a single procedure to generate functional hi ps cs-derived liver o...
Abstract : a single procedure to generate functional hi ps cs-derived liver o...
 
Poster – Development and automation of 3D innovative hiPSC-based liver organo...
Poster – Development and automation of 3D innovative hiPSC-based liver organo...Poster – Development and automation of 3D innovative hiPSC-based liver organo...
Poster – Development and automation of 3D innovative hiPSC-based liver organo...
 
Poster – Development and automation of 3D innovative hiPSC-based liver organo...
Poster – Development	and	automation of 3D innovative	hiPSC-based liver organo...Poster – Development	and	automation of 3D innovative	hiPSC-based liver organo...
Poster – Development and automation of 3D innovative hiPSC-based liver organo...
 
Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...
Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...
Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...
 
HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...
HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...
HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...
 
HCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® Brain
HCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® BrainHCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® Brain
HCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® Brain
 
A groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffold
A groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffoldA groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffold
A groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffold
 

Recently uploaded

trending-flavors-and-ingredients-in-salty-snacks-us-2024_Redacted-V2.pdf
trending-flavors-and-ingredients-in-salty-snacks-us-2024_Redacted-V2.pdftrending-flavors-and-ingredients-in-salty-snacks-us-2024_Redacted-V2.pdf
trending-flavors-and-ingredients-in-salty-snacks-us-2024_Redacted-V2.pdfMintel Group
 
business environment micro environment macro environment.pptx
business environment micro environment macro environment.pptxbusiness environment micro environment macro environment.pptx
business environment micro environment macro environment.pptxShruti Mittal
 
Send Files | Sendbig.comSend Files | Sendbig.com
Send Files | Sendbig.comSend Files | Sendbig.comSend Files | Sendbig.comSend Files | Sendbig.com
Send Files | Sendbig.comSend Files | Sendbig.comSendBig4
 
20200128 Ethical by Design - Whitepaper.pdf
20200128 Ethical by Design - Whitepaper.pdf20200128 Ethical by Design - Whitepaper.pdf
20200128 Ethical by Design - Whitepaper.pdfChris Skinner
 
Darshan Hiranandani [News About Next CEO].pdf
Darshan Hiranandani [News About Next CEO].pdfDarshan Hiranandani [News About Next CEO].pdf
Darshan Hiranandani [News About Next CEO].pdfShashank Mehta
 
EUDR Info Meeting Ethiopian coffee exporters
EUDR Info Meeting Ethiopian coffee exportersEUDR Info Meeting Ethiopian coffee exporters
EUDR Info Meeting Ethiopian coffee exportersPeter Horsten
 
MAHA Global and IPR: Do Actions Speak Louder Than Words?
MAHA Global and IPR: Do Actions Speak Louder Than Words?MAHA Global and IPR: Do Actions Speak Louder Than Words?
MAHA Global and IPR: Do Actions Speak Louder Than Words?Olivia Kresic
 
Investment in The Coconut Industry by Nancy Cheruiyot
Investment in The Coconut Industry by Nancy CheruiyotInvestment in The Coconut Industry by Nancy Cheruiyot
Investment in The Coconut Industry by Nancy Cheruiyotictsugar
 
APRIL2024_UKRAINE_xml_0000000000000 .pdf
APRIL2024_UKRAINE_xml_0000000000000 .pdfAPRIL2024_UKRAINE_xml_0000000000000 .pdf
APRIL2024_UKRAINE_xml_0000000000000 .pdfRbc Rbcua
 
WSMM Technology February.March Newsletter_vF.pdf
WSMM Technology February.March Newsletter_vF.pdfWSMM Technology February.March Newsletter_vF.pdf
WSMM Technology February.March Newsletter_vF.pdfJamesConcepcion7
 
NAB Show Exhibitor List 2024 - Exhibitors Data
NAB Show Exhibitor List 2024 - Exhibitors DataNAB Show Exhibitor List 2024 - Exhibitors Data
NAB Show Exhibitor List 2024 - Exhibitors DataExhibitors Data
 
Fordham -How effective decision-making is within the IT department - Analysis...
Fordham -How effective decision-making is within the IT department - Analysis...Fordham -How effective decision-making is within the IT department - Analysis...
Fordham -How effective decision-making is within the IT department - Analysis...Peter Ward
 
Ten Organizational Design Models to align structure and operations to busines...
Ten Organizational Design Models to align structure and operations to busines...Ten Organizational Design Models to align structure and operations to busines...
Ten Organizational Design Models to align structure and operations to busines...Seta Wicaksana
 
Call US-88OO1O2216 Call Girls In Mahipalpur Female Escort Service
Call US-88OO1O2216 Call Girls In Mahipalpur Female Escort ServiceCall US-88OO1O2216 Call Girls In Mahipalpur Female Escort Service
Call US-88OO1O2216 Call Girls In Mahipalpur Female Escort Servicecallgirls2057
 
TriStar Gold Corporate Presentation - April 2024
TriStar Gold Corporate Presentation - April 2024TriStar Gold Corporate Presentation - April 2024
TriStar Gold Corporate Presentation - April 2024Adnet Communications
 
Innovation Conference 5th March 2024.pdf
Innovation Conference 5th March 2024.pdfInnovation Conference 5th March 2024.pdf
Innovation Conference 5th March 2024.pdfrichard876048
 
8447779800, Low rate Call girls in Saket Delhi NCR
8447779800, Low rate Call girls in Saket Delhi NCR8447779800, Low rate Call girls in Saket Delhi NCR
8447779800, Low rate Call girls in Saket Delhi NCRashishs7044
 

Recently uploaded (20)

trending-flavors-and-ingredients-in-salty-snacks-us-2024_Redacted-V2.pdf
trending-flavors-and-ingredients-in-salty-snacks-us-2024_Redacted-V2.pdftrending-flavors-and-ingredients-in-salty-snacks-us-2024_Redacted-V2.pdf
trending-flavors-and-ingredients-in-salty-snacks-us-2024_Redacted-V2.pdf
 
business environment micro environment macro environment.pptx
business environment micro environment macro environment.pptxbusiness environment micro environment macro environment.pptx
business environment micro environment macro environment.pptx
 
Send Files | Sendbig.comSend Files | Sendbig.com
Send Files | Sendbig.comSend Files | Sendbig.comSend Files | Sendbig.comSend Files | Sendbig.com
Send Files | Sendbig.comSend Files | Sendbig.com
 
20200128 Ethical by Design - Whitepaper.pdf
20200128 Ethical by Design - Whitepaper.pdf20200128 Ethical by Design - Whitepaper.pdf
20200128 Ethical by Design - Whitepaper.pdf
 
Darshan Hiranandani [News About Next CEO].pdf
Darshan Hiranandani [News About Next CEO].pdfDarshan Hiranandani [News About Next CEO].pdf
Darshan Hiranandani [News About Next CEO].pdf
 
EUDR Info Meeting Ethiopian coffee exporters
EUDR Info Meeting Ethiopian coffee exportersEUDR Info Meeting Ethiopian coffee exporters
EUDR Info Meeting Ethiopian coffee exporters
 
Corporate Profile 47Billion Information Technology
Corporate Profile 47Billion Information TechnologyCorporate Profile 47Billion Information Technology
Corporate Profile 47Billion Information Technology
 
MAHA Global and IPR: Do Actions Speak Louder Than Words?
MAHA Global and IPR: Do Actions Speak Louder Than Words?MAHA Global and IPR: Do Actions Speak Louder Than Words?
MAHA Global and IPR: Do Actions Speak Louder Than Words?
 
Investment in The Coconut Industry by Nancy Cheruiyot
Investment in The Coconut Industry by Nancy CheruiyotInvestment in The Coconut Industry by Nancy Cheruiyot
Investment in The Coconut Industry by Nancy Cheruiyot
 
APRIL2024_UKRAINE_xml_0000000000000 .pdf
APRIL2024_UKRAINE_xml_0000000000000 .pdfAPRIL2024_UKRAINE_xml_0000000000000 .pdf
APRIL2024_UKRAINE_xml_0000000000000 .pdf
 
WAM Corporate Presentation April 12 2024.pdf
WAM Corporate Presentation April 12 2024.pdfWAM Corporate Presentation April 12 2024.pdf
WAM Corporate Presentation April 12 2024.pdf
 
WSMM Technology February.March Newsletter_vF.pdf
WSMM Technology February.March Newsletter_vF.pdfWSMM Technology February.March Newsletter_vF.pdf
WSMM Technology February.March Newsletter_vF.pdf
 
NAB Show Exhibitor List 2024 - Exhibitors Data
NAB Show Exhibitor List 2024 - Exhibitors DataNAB Show Exhibitor List 2024 - Exhibitors Data
NAB Show Exhibitor List 2024 - Exhibitors Data
 
Fordham -How effective decision-making is within the IT department - Analysis...
Fordham -How effective decision-making is within the IT department - Analysis...Fordham -How effective decision-making is within the IT department - Analysis...
Fordham -How effective decision-making is within the IT department - Analysis...
 
Ten Organizational Design Models to align structure and operations to busines...
Ten Organizational Design Models to align structure and operations to busines...Ten Organizational Design Models to align structure and operations to busines...
Ten Organizational Design Models to align structure and operations to busines...
 
Call US-88OO1O2216 Call Girls In Mahipalpur Female Escort Service
Call US-88OO1O2216 Call Girls In Mahipalpur Female Escort ServiceCall US-88OO1O2216 Call Girls In Mahipalpur Female Escort Service
Call US-88OO1O2216 Call Girls In Mahipalpur Female Escort Service
 
Japan IT Week 2024 Brochure by 47Billion (English)
Japan IT Week 2024 Brochure by 47Billion (English)Japan IT Week 2024 Brochure by 47Billion (English)
Japan IT Week 2024 Brochure by 47Billion (English)
 
TriStar Gold Corporate Presentation - April 2024
TriStar Gold Corporate Presentation - April 2024TriStar Gold Corporate Presentation - April 2024
TriStar Gold Corporate Presentation - April 2024
 
Innovation Conference 5th March 2024.pdf
Innovation Conference 5th March 2024.pdfInnovation Conference 5th March 2024.pdf
Innovation Conference 5th March 2024.pdf
 
8447779800, Low rate Call girls in Saket Delhi NCR
8447779800, Low rate Call girls in Saket Delhi NCR8447779800, Low rate Call girls in Saket Delhi NCR
8447779800, Low rate Call girls in Saket Delhi NCR
 

Neurotoxicity assay using High Content Screening technology

  • 1. Neurotoxicity assay using High Content Screening technology N. Maubon1*, M. Roudaut1, N. Gueguen1, and J. Bursztyka1 1 HCS Pharma, Biopôle, 6 rue Pierre Joseph Colin, 35000 Rennes 1*nathalie.maubon@hcs-pharma.com Abstract As shown by AstraZeneca in nature reviews*, one third of the safety failures is linked to CNS toxicity during the clinical trials of drugs . To avoid this attrition, the potential neurotoxicity of any drug going through the blood brain barrier (BBB) needs to be checked and if possible at the early stage of the research process for new chemical entites (NCE). This assay can be performed by cell imaging in HCA/HCS. Methods Results Conclusions & Perspectives  Neurotoxicity of compounds as rotenone or colchicine show that neurite length decreased in higher extent and/or at lower concentration compared to death of neuronal cells. For example, neurite length decreased with colchicine at 0.01 µM while neuronal cell death appeared at 0.1 µM.  Some other compounds as Methylmercure show that neuronal cells are only impacted, showing high variability in neurite length. At high concentration of Methylmercure (at 100 µM for example), neurites already exist between neighbored resistant cells with high activity of mitochondria.  The last 2 other compounds (lead (PbCl2) and Valinomycine) showed toxicity in the same extent between neuronal cells and neurites.  Conclusion : Neurotoxicity of compounds can be due to neuronal cell death but also due to loss of communication between the neuronal cells and depending of neurotoxic compounds, the loss of communication (neurites) can appear at lowest dose than neuronal cell death. This model of neurotoxicity performed in HCS can be a good model during the early stage of research. Cell culture: neuronal cells (SH-SY5Y) were routinely maintained in MEM/F12 (v/v) supplemented with 10% serum. Neuronal cells were seeded at 10 000 cells/well in 96-well Corning cellBind plates in MEM/F12 supplemented with differentiation agents. Then, cells were incubated at 37 °C in 5 % CO2 for 3 days for plating and differentiation. Treatment assay : Neurotoxicity assay was performed by replaced medium in each well by fresh medium with the different compounds to be tested at different concentrations. After 48h of incubation, cells were incubated with mitotracker during 30 min, fixed and then stained with b III tubulin and Hoechst. Image acquisition was performed on Operetta plateform (Perkin Elmer) and the analysis of cell number and neurite length was done through Columbus software (Perkin Elmer). To get this poster, please flash the QR- code You can use the I-NIGMA application from your store * Cook D, Brown D, Alexander R, March R, Morgan P, Satterthwaite G, Pangalos MN (2014) Lessons learned from the fate of AstraZeneca’s drug pipeline: a five-imensional framework. Nat Rev Drug Discov. 13(6):419-31. CTRL Rotenone Colchicine Lead Me-mercure Valinomycine Numberofcells Neuritelength Merge With brightfield bIII-tubulin Hoechst Mitotracker Hoechst Merge