Traditional covalent chemistries use harsh chemicals and require expertise in the techniques of diverse covalent coupling methodologies. The Antibody Coupling Kit was made to address issues such as: difficulties in binding certain antibodies with traditional covalent chemistries, antibody wastage, and incorrect antibody orientation. Anteo’s technology offers scientists the flexibility to bind any antibody onto a solid support surface through the use of polymeric metal complexes. The polymeric metal nature of the technology allows multi-valent binding of the target antibody through chelation to the electron donating groups located in the Fc region of the antibody. Anteo’s kit promotes gentle monolayer binding, meaning proteins assemble in the correct orientation while reducing the amount of damaged proteins, leading to increased functionality of antibodies and less antibodies used for the experiment.
This application note demonstrates the ability of the Antibody Coupling Kit to bind Mouse IgG, Mouse IgM, Rabbit IgG, Human IgG and Human IgM antibodies onto 200 nm magnetic particles using a particle-based fluorescent antibody loading assay.
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
it helps to known about ELISA ,it is one of the technique,, its type , its benefits & disadvantages , it also help to known about antigen,antidody, immunity etc
Dr. ihsan edan abdulkareem alsaimary
PROFESSOR IN MEDICAL MICROBIOLOGY AND MOLECULAR IMMUNOLOGY
ihsanalsaimary@gmail.com
mobile : 009647801410838
university of basrah - college of medicine - basrah -IRAQ
it helps to known about ELISA ,it is one of the technique,, its type , its benefits & disadvantages , it also help to known about antigen,antidody, immunity etc
The following presentation contains helpful information regarding Radioimmunoassay (RIA) and Enzyme-Linked Immunosorbent Assay (ELISA), including their history, introduction, advantages, procedures and applications.
ELISA or Enzyme-linked Immunosorbent Assay is a qualitative and quantitative assay for detecting the presence of antigens (virus, hormones, enzymes, etc.) in a sample.
serology presentation
Serology is the scientific study of blood serum and other bodily fluids such as semen and saliva.
In practical immunological terms, serology is the diagnostic identification of antibodies in the serum.
Antibodies are typically formed in response to;
An infection, (against a given microorganism),
Other foreign proteins (blood transfusion)
Or to one’s own proteins (autoimmune disease).
The presentation describes the basic principle and methodology to perform the technique of Radioimmunoassay (RIA) in detection of various drugs and their derivatives from various forensic specimens.
The following presentation contains helpful information regarding Radioimmunoassay (RIA) and Enzyme-Linked Immunosorbent Assay (ELISA), including their history, introduction, advantages, procedures and applications.
ELISA or Enzyme-linked Immunosorbent Assay is a qualitative and quantitative assay for detecting the presence of antigens (virus, hormones, enzymes, etc.) in a sample.
serology presentation
Serology is the scientific study of blood serum and other bodily fluids such as semen and saliva.
In practical immunological terms, serology is the diagnostic identification of antibodies in the serum.
Antibodies are typically formed in response to;
An infection, (against a given microorganism),
Other foreign proteins (blood transfusion)
Or to one’s own proteins (autoimmune disease).
The presentation describes the basic principle and methodology to perform the technique of Radioimmunoassay (RIA) in detection of various drugs and their derivatives from various forensic specimens.
Aakash Namkeen Pvt. Ltd. is one of the distinguished and leading manufacturer and exporters of namkeen (savories) and sweets from India.With the mantra of Quality, Aakash Namkeen has combated sensitive market conditions and cut-throat competition and has always emerged as a leader.
The Anteo Magnetic Separation Coupling Kit allows the rapid development of new tools for researchers. Utilising water-based chemistry, Anteo technology reduces hazardous chemical exposure. It allows flexibility in the selection of the particle of choice and ligand of interest. The chemistry also allows the development of multi-functional coupled particles with easy titration of ligand coupling concentration. The monolayer binding mechanism is highly efficient, reducing development time and costs. Use this kit to develop novel applications on magnetic particles - from antibody coupling, to antigens, peptides, carbohydrates, viruses, bacteria and cell surface markers.
In this application note, we show a range of proteins covering several species and isotypes of antibodies, as well as bacterial recombinant proteins which can be successfully coupled to Anteo activated particles with minimal optimisation. We also show that different proteins do have different affinities for Anteo coupling, and can be titrated to produce multifunctional particles. This monolayer protein coating results in improved functionality, which may save precious protein and reduce costs.
Lateral Flow: Making Magnetic Particles A Viable And Easier To Use Replacemen...Anteo Technologies
This application note titled Lateral Flow: Making Magnetic Particles A Viable And Easier To Use Replacement To Gold Nanoparticles outlines the preparation of 200nm magnetic particles conjugates for use in an anti-hepatitis B surface antigen based lateral flow assay.
Disruptive technology from Anteo Technologies for Lateral Flow testing. New product launching at AACC Conference July 2016. Five times improvement in sensitivity
The main purpose of these slides is to convey information to the Professors, Lecturers, and Students. These slides contain authentic information about this topic which is mentioned in that.
Achieving 5 x More Sensitivity And Less Variation In Results Than Covalent Ch...Anteo Technologies
A new method of conjugating antibodies to particles was compared to covalent conjugation in a lateral flow assay for human Chorionic Gonadotropin (hCG).
Anteo Technologies has launched a new Lateral Flow Coupling Kit that contains Anteo’s patented Activation Reagent with all necessary buffers for conjugating antibodies to a range of particles used for lateral flow applications.
In this paper, we demonstrate the performance advantages of using activated magnetic particles, only requiring the addition of antibody solution and blocking. Keeping all other variables constant, this kit was directly compared to covalently (EDAC chemistry) conjugated magnetic particles of the same type using visual detection as the readout.
In this hCG study, this coupling kit achieves five times more sensitivity than the covalently conjugated magnetic particle based assay using half the amount of antibody.
Characterization of intact antibodies by pre-fractionation using gel electrop...Expedeon
Antibodies represent an important class of proteins due to their central role in the immune response. Moreover, there is an increasing interest in the use of recombinant antibodies as novel drug therapies.
Novel composite electrodes:Preparation and application to the electroanalytic...Université de Dschang
M. Tchieno Melataguia Francis Merlin a soutenu une thèse de Doctorat/Phd en Chimie Inorganique ce 06 juin 2016 dans la salle des conférences de l'Université de Dschang. A l'issue de cette soutenance devant le jury présidé par le Prof. Emmanuel Ngameni lui a décerné la mention très honorable à l'unanimité de ses membres.
Discussing advances in Magnetic Bead coating technologies - Page 9 & 10 - Article from Joshua Soldo from Australian listed Biotech company Anteo Diagnostics ASX:ADO
Following is my journal documentation during Master's in Biotechnology completed in 2015. I do understand many changes would've occurred in the curriculum since then, but the basics seldom change. Kindly absorb as per your need.
Comparing Evolved Extractive Text Summary Scores of Bidirectional Encoder Rep...University of Maribor
Slides from:
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Track: Artificial Intelligence
https://www.etran.rs/2024/en/home-english/
What is greenhouse gasses and how many gasses are there to affect the Earth.moosaasad1975
What are greenhouse gasses how they affect the earth and its environment what is the future of the environment and earth how the weather and the climate effects.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Observation of Io’s Resurfacing via Plume Deposition Using Ground-based Adapt...Sérgio Sacani
Since volcanic activity was first discovered on Io from Voyager images in 1979, changes
on Io’s surface have been monitored from both spacecraft and ground-based telescopes.
Here, we present the highest spatial resolution images of Io ever obtained from a groundbased telescope. These images, acquired by the SHARK-VIS instrument on the Large
Binocular Telescope, show evidence of a major resurfacing event on Io’s trailing hemisphere. When compared to the most recent spacecraft images, the SHARK-VIS images
show that a plume deposit from a powerful eruption at Pillan Patera has covered part
of the long-lived Pele plume deposit. Although this type of resurfacing event may be common on Io, few have been detected due to the rarity of spacecraft visits and the previously low spatial resolution available from Earth-based telescopes. The SHARK-VIS instrument ushers in a new era of high resolution imaging of Io’s surface using adaptive
optics at visible wavelengths.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...
Antibody Coupling - Single Coupling Approach To Bind Antibodies Diverse Species And Classes
1.
Page 1
A Single Coupling Approach To Bind Antibodies Of Diverse
Species And Classes
Introduction
Antibody coupling plays a critical role in the life sciences. The ability to bind a range of different types of
antibodies functionally onto magnetic particles using a single chemistry frees scientists’ time for further
investigative studies. There are many applications possible using an antibody coupled particle such as
lateral flow, immunoprecipitation, purification or depletion of biomolecules and pull-down assays.
Scientists are often required to test different chemistries or methodologies to identify the one best
suited to a given antibody.
Passive adsorption is an example of a possible method of antibody attachment. This utilises
hydrophobic interactions to facilitate binding onto the hydrophobic solid support surface such as
polystyrene. As a result, there is no control of the orientation of the antibody resulting in reduced overall
functionality. Covalent chemistries offer another approach for coupling antibody that involve
modification of the surface to target the amino groups of the antibody. However if excess unreacted
reagent is not removed during the conjugation, the carboxyl groups of the antibody can become
activated [1] leading to antibody crosslinking instead of antibody-particle coupling. Anteo offers an
enabling technology that combines the ease of handling of passive adsorption with the strength of
binding of covalent chemistry.
Anteo’s surface coating technology allows the user to bind a range of different antibody types using the
Antibody Coupling Kit. The kit contains all of the reagents and buffers necessary to allow the user to
couple their required antibody simply and easily.
Summary
Traditional covalent chemistries use harsh chemicals and require expertise in the techniques of diverse
covalent coupling methodologies. The Antibody Coupling Kit was made to address issues such as:
difficulties in binding certain antibodies with traditional covalent chemistries, antibody wastage, and
incorrect antibody orientation. Anteo’s technology offers scientists the flexibility to bind any antibody
onto a solid support surface through the use of polymeric metal complexes. The polymeric metal nature
of the technology allows multi-valent binding of the target antibody through chelation to the electron
donating groups located in the Fc region of the antibody. Anteo’s kit promotes gentle monolayer
binding, meaning proteins assemble in the correct orientation while reducing the amount of damaged
proteins, leading to increased functionality of antibodies and less antibodies used for the experiment.
This application note demonstrates the ability of the Antibody Coupling Kit to bind Mouse IgG, Mouse
IgM, Rabbit IgG, Human IgG and Human IgM antibodies onto 200 nm magnetic particles using a
particle-based fluorescent antibody loading assay.
AN1 – ANTIBODY COUPLING KIT
2.
Page 2
Materials And Methods
Antibody Coupling Kit (Cat # A-VMPABMP-50):
- Particle Pretreatment Solution
- Particle Activation Solution
- Particle Intermediate Solution
- Coupling Buffer
- Blocking Buffer
- Storage Buffer
• AllMag SuperParamagnetic Nanoparticles
(Allrun, Cat # PM3-020)
• Assay Buffer: 1% BSA in 10mM PBS + 0.05%
Tween 20 pH 7.4
• Wash Buffer: 10mM PBS + 0.05% Tween 20
pH 7.4
• Plate: 96-well white polypropylene round
bottom plate (Greiner, Cat #: 650207)
• Plate magnet: BioMag® 96-Well Plate
Separator (Polysciences Inc., cat #:
8MB4109S-1)
• Readout: Tecan Infinite M200 Pro (Tecan)
Table 1: Antibodies used for coupling and their
respective detection reagent
Antibody For Coupling Detection Reagent
Mouse IgM (Lampire,
Cat # 7404317)
R-Phycoerythrin
AffiniPure F(ab')2
Fragment Goat Anti-
Mouse IgM, µ Chain
Specific (Jackson, Cat #
115-116-075)
Mouse IgG (Lampire,
Cat # 7404304)
R-Phycoerythrin
AffiniPure F(ab') 2
Fragment Goat Anti-
Mouse IgG, F(ab') 2
Fragment Specific
(Jackson, Cat # 115-
116-072)
Rabbit IgG (Lampire,
Cat # 7406404)
R-Phycoerythrin
AffiniPure F(ab') 2
Fragment Goat Anti-
Rabbit IgG (H+L)
(Jackson, Cat # 111-
116-144)
Antibody For Coupling Detection Reagent
Human IgG (Lampire,
Cat # 7403704)
R-Phycoerythrin
AffiniPure F(ab') 2
Fragment Goat Anti-
Human IgG (H+L)
(Jackson, Cat # 109-
116-088)
Magnetic Particle Activation And Coupling
Magnetic particle activation and coupling were
prepared as per the Antibody Coupling Kit
instructions and detailed below.
Preparation For Particle Activation
The magnetic particles were resuspended by
vortex and sonication. An aliquot of 1 mg of the
magnetic particles was taken and then placed on
a magnet to remove the supernatant. The
magnetic particles were resuspended in Particle
Pretreatment Solution, with a vortex of the tube
to ensure that the contents were dispersed.
Particle Activation
To a fresh tube, 90% of the final required volume
for the antibody coupling was added with
Particle Activation Solution. The magnetic
particles were then added to the tube containing
the Particle Activation solution. The magnetic
particles were resuspended with a vortex, this
was then followed by sonication of the tube to
ensure that the contents were dispersed prior to
incubation at room temperature. After incubation
of the magnetic particles, the Particle Activation
Solution was removed and replaced with Particle
Intermediate Solution.
3.
Page 3
Antibody Coupling And Blocking
The supernatant from the activated magnetic
particles was removed. The magnetic particles
were then resuspended with Coupling Buffer,
with a vortex of the tube to ensure that the
contents were dispersed.
These steps were repeated twice, resulting in a
total of three washes. In a new tube, the required
antibody was prepared to ensure that the final
concentration would be at 50 µg of antibody /mg
beads. The magnetic particles were then added
to the tube containing the antibody. The
magnetic particles were resuspended with a
vortex, this was then followed by sonication of
the tube to ensure that the contents were
dispersed prior to incubation at room
temperature.
Following the incubation period, blocking buffer
was added to the magnetic particle solution. The
magnetic particles were then vortexed and
allowed to incubate. After the incubation period,
the supernatant of the magnetic particles was
removed and replaced with Storage Buffer. The
magnetic particles were washed again before
resuspending at the starting particle
concentration.
Assessment Of Antibody Coupling By
Fluorescent Bead-based Assay
The antibody coupled magnetic particles were
diluted using 1% BSA in 10mM PBS + 0.05%
Tween20 pH 7.4. 50 µL of the antibody coupled
magnetic particles were subsequently added to a
round-bottomed 96-well plate. The detection
reagents (refer to Table 1) were prepared at
10µg/mL using 1% BSA in 10mM PBS + 0.05%
Tween20 pH 7.4. 50 µL of the detection reagent
was added to the round-bottomed 96-well plate.
As a result of the R-Phycoerythrin fluorophore
label being light sensitive, the plate was covered
from light and was allowed to incubate at room
temperature.
Following the incubation period, the plate was
placed on a magnet with the supernatant
removed. The magnetic particles were then
resuspended in 100 µL of 10mM PBS + 0.05%
Tween20 pH 7.4. These steps were repeated
four times, resulting in a total of five washes. The
plate containing the magnetic particles was
measured using a Tecan infinite M200 PRO plate
reader (refer to Table 2).
Table 2: Tecan infinite M200 PRO plate reader
configuration
Variables Output
Mode
Fluorescence Top
Reading
Excitation Wavelength 546 nm
Emission Wavelength 575 nm
Excitation Bandwidth 9 nm
Emission Bandwidth 20 nm
Gain 72
Results
The following figures 1, 2 and 3 show the results
obtained through the use of a fluorescent bead-
based assay. From the data, binding of the
detection reagent to the antibody coupled
magnetic particles was achieved. This occurred
across all the different species and classes of
antibodies tested. The use of assay buffer alone
did not show any observable background
binding.
4.
Page 4
Figure 1: Fluorescent antibody loading assay of IgA coupling on magnetic particles. Replicates
showing both specific binding output and blank assay background results for the range of specific RPE
labeled detection reagents.
Figure 2: Fluorescent antibody loading assay of IgM coupling on magnetic particles. Replicates
showing both specific binding output and blank assay background results for the range of specific RPE
labeled detection reagents.
5.
Page 5
Figure 3: Fluorescent antibody loading assay of IgG coupling on magnetic particles. Replicates
showing both specific binding output and blank assay background results for the range of specific RPE
labeled detection reagents.
Discussion
Anteo’s technology utilises metal polymer complexes. The polymeric nature of the technology allows
multi-valent binding of the target antibody through chelation to the electron donating groups of the
antibody which results in a gentle yet strong approach to coupling antibodies [2]. In contrast, covalent
chemistries offer strong bonds that may impact the antibody structure. Both passive adsorption and
covalent chemistries often suffer from loss of activity due to the antibody structure being deformed or
the active site of the antibody being shielded [3].
A panel of five different antibodies was selected to cover a wide range of immunoglobulin types (IgA,
IgG and IgM) and species sources (mouse, rabbit and human) as shown in figures 1, 2 and 3.. To
highlight the flexibility of the Antibody Coupling Kit, all of the antibodies that were tested were coupled
at the same antibody concentration and assayed under the same conditions. The antibodies that were
used in this experiment had varying primary, secondary and tertiary structure as well as differences in
species and antibody class types. The IgG antibodies consists of two heavy polypeptide chains and
two light polypeptide chains, this forms a ‘Y’ shaped monomeric structure. The IgA antibodies are
found as a dimer structure, while IgM antibodies exists as a pentamer. At the same volume, the
differences in the size of the antibody structure will affect the number of moles of antibody present. As
a result, the molar concentrations present in each antibody condition were different, accounting for the
differences in signal observed in the loading assays. An example of the differences observed among
antibodies is IgG. Between species there is a conserved region through the N-glycosylation site in the
heavy chain of the Fc region [4]. The oligosaccharides present at this site are highly heterogenous and
have been found to affect the biological, pharmacological and physicochemical properties of IgGs [5].
These subtle differences between antibodies may require further antibody specific optimisation to
account for variations in the electron density groups available to co-ordinate to the metal polymer
complex.
6.
Page 6
Conclusion
A wide range of antibodies are used in the life sciences. In the past, the heterogeneous nature of
antibodies and their properties often required pre-screening against several binding chemistries to
select the most compatible for a given antibody. In contrast, Anteo’s Antibody Coupling Kit enables the
use of a broad range of different antibodies using a single chemistry.
Traditional covalent binding chemistries use harsh chemicals that may impact the antibody function [6].
Whilst with the use of Anteo’s unique technology, antibodies are bound through its multi-component
binding property; this gentle approach results in very high antibody function. The binding that is
observed with the Antibody Coupling Kit is a highlight of the ease with which a result can be obtained.
The multiple benefits that are associated with using the Antibody Coupling Kit are the ease of use,
flexibility to bind any antibody to any particle from 200nm to 3µm magnetic, latex and slica particles,
reduction in background, reduced development time, more accurate and reproducible results. Anteo’s
technology allows scientists to successfully bind any antibody class and eliminates the need for
screening other compatible chemistries.
Contact Customer Support: If you have any questions about the Anteo Antibody Coupling Kit, contact,
support@anteotech.com or call +61 (0) 7 3219 0085.
Anteo’s products are for research use only.
References
[1] Bead Coupling Technology and Applications (Bio-Rad) n.d. [20 June 2016]
[2] Ooi, H., Cooper, S., Huang, C., Jennins, D., Chung., Maeji, N. & Whittaker, A. 2014, ‘Coordination
complexes as molecular glue for immobilization of antibodies on cyclic olefin copolymer surfaces’,
Analytical Biochemistry, vol. 456, pp. 6-13.
[3] Liu,Y & Yu,J. 2016, ‘Oriented immobilization of proteins on solid supports for use in biosensors and
biochips: a review’, Microchimica Acta, vol. 183, pp. 1-19.
[4] Sutton, B.J. & Phillips,D.C. 1983 ‘The three-dimensional structure of the carbohydrate within the Fc
fragment of immunoglobulin G. biochem. Soc. Trans., vol. 11, pp 130-132.
[5] Mizuochi,T., Taniguchi,T., Shimizu, A. & Kobata, A. 1982 ‘Structural and numerical variations of the
carbohydrate moiety of immunoglobulin G. J. Immunol., vol, 129 pp. 2016-2020.
[6] Campos, E., Coimbra, P. & Gil, M. 2012 ‘An improved method for preparing glutaraldehyde cross-
linked chitosan–poly(vinyl alcohol) microparticles’, Polymer Bulletin, vol. 70, 549-561.
.