The Australian Transect Network establishes long-term monitoring sites along environmental gradients across Australia to track changes in species composition, traits, and genes over time and space in response to climate change, using space as a proxy for time. Data from the transects are used to validate models, engage citizens in science, and inform policy decisions about landscape management and climate adaptation. The network includes transects in northern, southwestern, and southeastern Australia that monitor vegetation and wildlife along climatic and disturbance gradients.