The study optimized multi-layer-cum-microwave drying process parameters for oyster mushrooms, focusing on loading density, air velocity, and power level to improve product quality. The optimal conditions were found to be 3.80 m/sec air velocity, 38.80 kg/m² loading density, and 413 W power level, yielding a rehydration ratio of 2.15 and an overall desirability of 0.78. Quality assessments showed significant improvements in texture, color, and overall acceptability of the dried mushrooms using the optimized drying method.