The document discusses laboratory techniques for generating high-quality genome assemblies, including PacBio long-read sequencing, 10X Genomics linked reads, and BioNano physical mapping. PacBio sequencing of various library preparation methods produced reads over 10kb in length. 10X Genomics linked reads provided long-range phasing information to resolve alleles from repeats. BioNano mapping revealed a large inversion in one genome through detection of nick sites. The integration of these long-read and long-range techniques aims to capture more human genetic diversity in reference genomes.