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ABO and Rh typing
Dr. HOSSAM FAHMY
ABO & Rh(D) 1
The ABO System
• Discovered in 1901 by Dr. Karl
Landsteiner
• 4 Main Phenotypes (A, B, AB,
O)
ABO & Rh(D) 2
ABO & Rh(D) 3
ABO & Rh(D) 4
Antigens & Antibodies
Blood Group
Antigens on
RBCs
Antibodies in Serum Genotypes
A A Anti-B AA or AO
B B Anti-A BB or BO
AB A and B Neither AB
O Neither Anti-A and anti-B OO
ABO & Rh(D) 5
Inheritance of ABO Groups
Allele from
the mother
Allele from
the father
Genotype of
offspring
Blood types of
offspring
A A AA A
A B AB AB
A O AO A
B A AB AB
B B BB B
B O BO B
O O OO O
ABO & Rh(D) 6
ABO Typing
• Cell Group
– Test Washed Cells
With:
– Monoclonal Anti-A
– Monoclonal Anti-B
– Inert control
• Agglutination is a
positive result
• Reverse Group
– Test plasma/serum
with:
– Known A cells
– Known B cells
– Known O cells
• Reactions may be
weaker than cell
group
ABO & Rh(D) 7
ABO & Rh(D) 8
Distribution of ABO Groups
ABO & Rh(D) 9
The Rh Antigen
• Rh is the most complex system, with
over 45 antigens
• Discovered in 1940 after work on
Rhesus monkeys
• Subsequently discovered to be
unrelated to monkeys
ABO & Rh(D) 10
The Fisher System
ABO & Rh(D) 11
Simple Genetics of Rh(D)
• 86% of caucasians are Rh(D) pos
• The d gene is recessive:
– Dd, dD, DD, persons are Rh(D) pos
– Only dd persons are Rh(D) neg
ABO & Rh(D) 12
Distribution of Rh(D) Types
Population Rh(D) pos Rh(D) neg
Caucasian 86% 14%
African-
American
95% 5%
Oriental >99% <1%
ABO & Rh(D) 13
Significance of Rh(D)
• 80% of Rh(D) neg persons exposed to Rh(D)
pos blood will develop anti-D
• Anti-D can also be stimulated by pregnancy
with an Rh(D) positive baby
ABO & Rh(D) 14
ABO & Rh(D) 15
Sensitisation can be prevented by the use of
anti-D immunoglobulin.
Rh(D) neg females of childbearing potential
should never be given Rh(D) positive blood
products.
ABO & Rh(D) 16
A Rh(D) + mother B Rh(D) + father
Could they have an O Rh(D) – child ?
ABO & Rh(D) 17
Mother
Group A AO
Rh(D) pos Dd
Father
Group B BO
Rh(D) pos Dd
Group A AO
Rh(D) pos Dd
Group B BO
Rh(D) pos Dd
Group O OO
Rh(D) neg dd
+
ANTIGLOBULIN TEST
and
ANTIBODY DETECTION
ABO & Rh(D) 19
Detection of non-agglutinating Ab,
or complement components affixed
to RBCs in vivo or in vitro.
ABO & Rh(D) 20
– Direct antiglobulin test (DAT) -
detection of sensitization of RBC s
coated with Abs and/or complement
components in vivo
– Indirect antiglobulin test (IDAT) -
detection of sensitization of RBCs in
vitro
ABO & Rh(D) 21
ABO & Rh(D) 22
ABO & Rh(D) 23
Strength of the observed agglutination
is proportional to amount of Ab or
complement on RBCs
ABO & Rh(D) 24

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ABO RHESUS TYPING.pptx

  • 1. ABO and Rh typing Dr. HOSSAM FAHMY ABO & Rh(D) 1
  • 2. The ABO System • Discovered in 1901 by Dr. Karl Landsteiner • 4 Main Phenotypes (A, B, AB, O) ABO & Rh(D) 2
  • 4. ABO & Rh(D) 4 Antigens & Antibodies Blood Group Antigens on RBCs Antibodies in Serum Genotypes A A Anti-B AA or AO B B Anti-A BB or BO AB A and B Neither AB O Neither Anti-A and anti-B OO
  • 5. ABO & Rh(D) 5 Inheritance of ABO Groups Allele from the mother Allele from the father Genotype of offspring Blood types of offspring A A AA A A B AB AB A O AO A B A AB AB B B BB B B O BO B O O OO O
  • 6. ABO & Rh(D) 6 ABO Typing • Cell Group – Test Washed Cells With: – Monoclonal Anti-A – Monoclonal Anti-B – Inert control • Agglutination is a positive result • Reverse Group – Test plasma/serum with: – Known A cells – Known B cells – Known O cells • Reactions may be weaker than cell group
  • 8. ABO & Rh(D) 8 Distribution of ABO Groups
  • 9. ABO & Rh(D) 9 The Rh Antigen • Rh is the most complex system, with over 45 antigens • Discovered in 1940 after work on Rhesus monkeys • Subsequently discovered to be unrelated to monkeys
  • 10. ABO & Rh(D) 10 The Fisher System
  • 11. ABO & Rh(D) 11 Simple Genetics of Rh(D) • 86% of caucasians are Rh(D) pos • The d gene is recessive: – Dd, dD, DD, persons are Rh(D) pos – Only dd persons are Rh(D) neg
  • 12. ABO & Rh(D) 12 Distribution of Rh(D) Types Population Rh(D) pos Rh(D) neg Caucasian 86% 14% African- American 95% 5% Oriental >99% <1%
  • 13. ABO & Rh(D) 13 Significance of Rh(D) • 80% of Rh(D) neg persons exposed to Rh(D) pos blood will develop anti-D • Anti-D can also be stimulated by pregnancy with an Rh(D) positive baby
  • 15. ABO & Rh(D) 15 Sensitisation can be prevented by the use of anti-D immunoglobulin. Rh(D) neg females of childbearing potential should never be given Rh(D) positive blood products.
  • 16. ABO & Rh(D) 16 A Rh(D) + mother B Rh(D) + father Could they have an O Rh(D) – child ?
  • 17. ABO & Rh(D) 17 Mother Group A AO Rh(D) pos Dd Father Group B BO Rh(D) pos Dd Group A AO Rh(D) pos Dd Group B BO Rh(D) pos Dd Group O OO Rh(D) neg dd +
  • 19. ABO & Rh(D) 19 Detection of non-agglutinating Ab, or complement components affixed to RBCs in vivo or in vitro.
  • 20. ABO & Rh(D) 20 – Direct antiglobulin test (DAT) - detection of sensitization of RBC s coated with Abs and/or complement components in vivo – Indirect antiglobulin test (IDAT) - detection of sensitization of RBCs in vitro
  • 23. ABO & Rh(D) 23 Strength of the observed agglutination is proportional to amount of Ab or complement on RBCs