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A new successful wide cross between C. cajan (L.) Millsp.
and C. lanceolatus (WV Fitgz) van der Maesen
Sandhya Srikanth1,2
, M.V. Rao2
and Nalini Mallikarjuna1
1
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad 502 324, Andhra Pradesh, India
2
Bharathidasan University, Tiruchirappalli – 620 024, Tamil Nadu, India
Cultivated pigeonpea has a narrow genetic base.Wild relatives play an important role in broadening its genetic
base. The utilization of wild species from the secondary gene pool are important as they are closely related
leading to normal chromosome recombination. This helps in the transfer of useful genes/traits to cultivated
pigeonpea (Mallikarjuna et al., 2011). Until now C. lanceolatus had not been successfully crossed as previous
report obtained F1
hybrids, but they did not reach the reproductive stage.
MEIOTIC ANALYSIS OF F1
HYBRIDS
METAPHASE, ANAPHASE, TETRADS, POLLEN AND ANTHER OF FERTILE F1
P6
METAPHASE, ANAPHASE,TETRADS, POLLEN AND ANTHER OF STERILE HYBRID F1
P7
MORPHOLOGY OF LEAVES, FLOWERS AND SEEDS OF PARENTS AND HYBRIDS
Metaphase showing
univalents
Anaphase showing univalents and
unequal distribution
Tetrads showing
micronuclei
Non-seperation
of tetrads
Sterile anther
♦♦ Bruchid (Callosobruchus maculatus F) is well known pest
of pigeonpea in storage world wide. The present study
shows that C. lanceolatus is a good source of resistance
to bruchids. The larval/pupal period was prolonged in C.
lanceolatus(57-81days)comparedto35daysonthefemale
parent ICPL 85010 indicating the antibiosis mechanism of
resistance in the wild species. Many of the hybrids showed
different levels of resistance to bruchids.
S.NO PLANT NUMBER POLLEN FERTILITY %
1 ICPL 85010 X ICPW15639 F1
P1
0 (STERILE)
2 ICPL 85010 X ICPW15639 F1
P2
37.8
3 ICPL 85010 X ICPW15639 F1
P3
24.3
4 ICPL 85010 X ICPW15639 F1
P4
0 (STERILE)
5 ICPL 85010 X ICPW15639 F1
P5
40.5
6 ICPL 85010 X ICPW15639 F1
P6
40.7
7 ICPL 85010 X ICPW15639 F1
P7
0 (STERILE)
8 ICPL 85010 X ICPW15639 F1
P8
56
9 ICPL 85010 X ICPW15639 F1
P9
36.06
10 ICPL 85010 X ICPW15639 F1
P10
0 (STERILE)
11 ICPL 85010 X ICPW15639 F1
P11
45%
12 ICPL 85010 X ICPW15639 F1
P12
0 (STERILE)
13 ICPL 85010 X ICPW15639 F1
P13
50%
14 ICPL 85010 X ICPW15639 F1
P14
48%
PLANT NO
(ICPL85010
X ICPW
15639) F1
NO. OF
SEEDS
NO. OF
ADULTS
RELEASED
NO. OF
EGGS
LAID
NO. OF
ADULTS
EMERGED
NO.OF
DAYS
TAKEN
(Range)
ADULT
MORT
ALITY
%
P1
10 8 16 8 44-54 62.5
P2
10 8 38 12 41 -56 16.7
P4
10 8 4 3 44-56 33.3
P5
5 6 25 5 47-53 60
P6
5 8 13 6 41-51 33.3
P7
10 8 9 7 44-54 42.9
P8
5 8 19 1 56 100
ICPW 15639 12 10 29 10 57-81 33.3
ICPL 85010 10 6 14 10 35-38 0
C. maculatus laid eggs on
seeds of ICPW 15639
adult died inside the seed of
ICPW15639 (after 81 days )
n.mallikarjuna@cgiar.org
POLLEN FERTILITY IN HYBRIDS TABLE SHOWING THE BRUCHID ANALYSIS
ICPL 85010
HEIGHT-175 cm
F1
P14
F1
P13
F1
P12
F1
P11
F1
P10
ICPW 15639 ICPW 85010
ICPW 15639
HEIGHT-285 cm
ICPL 85010 X
ICPW 15639
F1
P11
Height-380 cm
Pods of ICPL 85010 Pods and Flowers of ICPW 15639
Flowers of F1
Hybrid
F1
Hybrid
ICPW 15639
ICPW 15639
ICPL 85010
ICPL 85010
ICPL 85010 ICPW 15639
Flowers of F1
Hybrid
Seeds of F1
Hybrid
ICPL 85010 X ICPW 15639 F1
P12
ICPW 15639ICPL 85010
(ICPL 85010 X ICPW 15639)
F1
P12
Sterile anthers
(ICPL 85010 X ICPW 15639)
F1
P6
Fertile anthers

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  • 1. A new successful wide cross between C. cajan (L.) Millsp. and C. lanceolatus (WV Fitgz) van der Maesen Sandhya Srikanth1,2 , M.V. Rao2 and Nalini Mallikarjuna1 1 International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad 502 324, Andhra Pradesh, India 2 Bharathidasan University, Tiruchirappalli – 620 024, Tamil Nadu, India Cultivated pigeonpea has a narrow genetic base.Wild relatives play an important role in broadening its genetic base. The utilization of wild species from the secondary gene pool are important as they are closely related leading to normal chromosome recombination. This helps in the transfer of useful genes/traits to cultivated pigeonpea (Mallikarjuna et al., 2011). Until now C. lanceolatus had not been successfully crossed as previous report obtained F1 hybrids, but they did not reach the reproductive stage. MEIOTIC ANALYSIS OF F1 HYBRIDS METAPHASE, ANAPHASE, TETRADS, POLLEN AND ANTHER OF FERTILE F1 P6 METAPHASE, ANAPHASE,TETRADS, POLLEN AND ANTHER OF STERILE HYBRID F1 P7 MORPHOLOGY OF LEAVES, FLOWERS AND SEEDS OF PARENTS AND HYBRIDS Metaphase showing univalents Anaphase showing univalents and unequal distribution Tetrads showing micronuclei Non-seperation of tetrads Sterile anther ♦♦ Bruchid (Callosobruchus maculatus F) is well known pest of pigeonpea in storage world wide. The present study shows that C. lanceolatus is a good source of resistance to bruchids. The larval/pupal period was prolonged in C. lanceolatus(57-81days)comparedto35daysonthefemale parent ICPL 85010 indicating the antibiosis mechanism of resistance in the wild species. Many of the hybrids showed different levels of resistance to bruchids. S.NO PLANT NUMBER POLLEN FERTILITY % 1 ICPL 85010 X ICPW15639 F1 P1 0 (STERILE) 2 ICPL 85010 X ICPW15639 F1 P2 37.8 3 ICPL 85010 X ICPW15639 F1 P3 24.3 4 ICPL 85010 X ICPW15639 F1 P4 0 (STERILE) 5 ICPL 85010 X ICPW15639 F1 P5 40.5 6 ICPL 85010 X ICPW15639 F1 P6 40.7 7 ICPL 85010 X ICPW15639 F1 P7 0 (STERILE) 8 ICPL 85010 X ICPW15639 F1 P8 56 9 ICPL 85010 X ICPW15639 F1 P9 36.06 10 ICPL 85010 X ICPW15639 F1 P10 0 (STERILE) 11 ICPL 85010 X ICPW15639 F1 P11 45% 12 ICPL 85010 X ICPW15639 F1 P12 0 (STERILE) 13 ICPL 85010 X ICPW15639 F1 P13 50% 14 ICPL 85010 X ICPW15639 F1 P14 48% PLANT NO (ICPL85010 X ICPW 15639) F1 NO. OF SEEDS NO. OF ADULTS RELEASED NO. OF EGGS LAID NO. OF ADULTS EMERGED NO.OF DAYS TAKEN (Range) ADULT MORT ALITY % P1 10 8 16 8 44-54 62.5 P2 10 8 38 12 41 -56 16.7 P4 10 8 4 3 44-56 33.3 P5 5 6 25 5 47-53 60 P6 5 8 13 6 41-51 33.3 P7 10 8 9 7 44-54 42.9 P8 5 8 19 1 56 100 ICPW 15639 12 10 29 10 57-81 33.3 ICPL 85010 10 6 14 10 35-38 0 C. maculatus laid eggs on seeds of ICPW 15639 adult died inside the seed of ICPW15639 (after 81 days ) n.mallikarjuna@cgiar.org POLLEN FERTILITY IN HYBRIDS TABLE SHOWING THE BRUCHID ANALYSIS ICPL 85010 HEIGHT-175 cm F1 P14 F1 P13 F1 P12 F1 P11 F1 P10 ICPW 15639 ICPW 85010 ICPW 15639 HEIGHT-285 cm ICPL 85010 X ICPW 15639 F1 P11 Height-380 cm Pods of ICPL 85010 Pods and Flowers of ICPW 15639 Flowers of F1 Hybrid F1 Hybrid ICPW 15639 ICPW 15639 ICPL 85010 ICPL 85010 ICPL 85010 ICPW 15639 Flowers of F1 Hybrid Seeds of F1 Hybrid ICPL 85010 X ICPW 15639 F1 P12 ICPW 15639ICPL 85010 (ICPL 85010 X ICPW 15639) F1 P12 Sterile anthers (ICPL 85010 X ICPW 15639) F1 P6 Fertile anthers