IONICS is a mass spectrometry company that has been innovating its triple quadrupole product line. It started with the 3Q Series A in 2010 and has since released two new generations, the 3Q Series 100 and 3Q Series 200, pushing the limits of sensitivity. IONICS' latest product is the 3Q Series 300, which provides even lower detection limits down to femtogram and attogram levels for various compounds. IONICS aims to continue innovating its products and working with industry leaders to advance the field.

1. IONICS Mass Spectrometry
- Boldy going….
- Triple Quad Mass Spectrometry
- A Big Year for IONICS
Erik Hansen
CPSA 2012

2. Global Headquarters
• Greater Toronto Area
(Bolton)
• Est 2001
• 40+ employees
• R&D, Manufacturing, Service, Marketing and
Sales
• Global distribution / OEM Partnerships

3. Evolution at IONICS
IONICS New Generation
IONICS - Prequel
Triple Quad – 3Q Series A
molecular Analyser

4. 3Q Series A Molecular Analyzer
• Flow-Based Mass Spectrometry
– Its like fuel injection vs
regular carburation
– Launched in 2010
– Innovations in
• HSID – Laminar Flow ION
Guide- Collision Cell Unifield™
Detector (combination)
– Vertical Design

5. Evolution Continues at IONICS
IONICS New Generation IONICS Next IONICS Latest Generation
IONICS - Upgrades 3Q Series 300
Series A Generation 3Q Series
3Q Molecular Analyzer 100, 3Q Series 200

6. IONICS Family of Triples
3Q 300
3Q 100
3Q 200
Mass Range
Mass Range Mass Range
5-800 m/z
5-2150 m/z 5-1500 m/z
Deep Space
Terrestrial Near Space

7. MolAna Software
MolAna LC-MRM Quantitation Software for the
High Performance 3Q Triple Quad Molecular
Analyzers.
Software features
• Easy to learn workflow
• Modularized data acquisition, batch
creation, data viewing and quantitation
• Import export capabilities for each module

8. 3Q 300 series
LC-MRM for Reserpine 609.2195.1 transition
25000
200
20000
150 100.0fg/μL
15000
100 500ag/μL S/N=5530
S/N=27 10000
50 5000
0 0
0 0.2 0.4 0.6 0.8 1 0 0.2 0.4 0.6 0.8 1
200 2500
100000
2000 10.0fg/μL
150
S/N=560 y = 569x - 35.54
1500
100 R² = 0.999
250ag/μL 1000
10000
50 S/N=13 500
Area (counts)
0 0
1000
0 0.2 0.4 0.6 0.8 1 0 0.2 0.4 0.6 0.8 1
200 1000
100
150 800
2.0fg/μL
600 S/N=110
100
Blank 400 10
50 200 0.1 1 10 100
0 Concentration
0
0 0.2 0.4 0.6 0.8 1
0 0.2 0.4 0.6 0.8 1
Shimadzu Prominence UFLC
Imtakt Cadenza CD-C18HT (50x2.1 mm, 3µm)
Isocratic 3/7 H2O/MeOH with 0.1 % Formic Acid
5 µL injection
Flow rate of 0.8 mL/min

9. 3Q 300 series
ECI: LC-MRM, Lowest limit of detection for Testosterone (298.497.1)
800
700 200 ag/μL
600 S/N= 11
Intensity (CPS)
500
400
300
200
100
LC condition: 2.8 2.9 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8
Shimadzu Prominence UFLC Time (min)
Kinetex C18 column (75x2.1 mm,2.6 µm)
8 min gradient from 5 to 95% MeOH in 10
mM NH4OAc and 0.1 % Formic Acid
Injection volume: 10 µL 800
Flow rate of 0.5 mL/min
700
Blank
600
Intensity (CPS)
500
400
300
200
100
2.8 2.9 3 3.1 3.2 3.3 3.4 3.5 3.6 3.7 3.8
Time (min)

10. 3Q 300 series
ESI: LC-MRM, Lowest limit of detection for AVP (542.9120.1) in plasma
200
180
160 2.5 fg/μL
140 S/N= 11
Intensity (CPS)
120
100
80
60
40
20
0
1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2
LC condition:
Time (min)
Shimadzu Prominence UFLC 200
model
180
6 min gradient run from 5 to
90% ACN in 0.05 % Formic 160 Blank
Acid 140
Intensity (CPS)
Thermo BetaBasic -C18 120
column, (30x2.1mm, 3µm)
100
Injection volume: 10µL
Flow rate of 0.4 mL/min 80
60
40
20
0
1 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2
Time (min)

11. 3Q 300 series
ECI: LC-MRM, Lowest limit of detection for Fentanyl (337.5188.1)
140 33 ag/μL
120
S/N= 20
100
Intensity (CPS)
80
60
40
20
0
1.2 1.3 1.4 1.5 1.6 1.7 1.8
Time (min)
140
120
Blank
Intensity (CPS)
100
80
LC condition: 60
40
Shimadzu Prominence UFLCxr model
Kinetex C18 column (75x2.1 mm, 2.6 µm) 20
5 min gradient from 10 to 95% MeOH in 10 0
mM NH4OAc and 0.1 % Formic Acid 1.2 1.3 1.4 1.5 1.6 1.7 1.8
Injection volume: 10 µL
Flow rate of 0.5 mL/min Time (min)

12. IONICS’ Current line of products
Testosterone (289.1/97.1) 10 fg/μL X8 1.25 fg/μL X5 0.25 fg/μL
S/N=11 S/N=11 S/N=11
Fentanyl (337.1/188) 1.5 fg/uL X6 250 ag/uL X8 32 ag/uL
S/N ≈ 7
Reserpine 5 fg/μL S/N=5 X6 S/N=32 X6 S/N=230
3Q 100 3Q 200 3Q 300

13. IONICS Mass Spectrometry
• Pushing the limits Sensitivity
– Micro-dosing?
• Working with Industry Thought Leaders
• Innovating at a serious pace - Series
100, Series 200, Series 300…
• Join us
Live long and prosper

14. Acknowledgements
IONICS Team
• Reza Javahery
• Lisa Cousins
• Chuck Joliffe
• Josh Ye
• Changtong Hao
• Hui Qiao
• Ellie Majdi
• Alex Taylor
• Heather Gamble
• George Scott
• Brian Clark
• Frenny Riparelli