5 minutes of fame

1. Everything That’s Old is New
Again
Introducing HALO-5
A Truly “High Performance”
5µ Particle

2. How Much Does a
Theoretical Plate Weigh?

3. Introduction
 HALO core-shell particles are unique high-purity silica
particles for stable high efficiency packed columns that
are capable of very fast separations with modest back
pressures
 Original HALO particles are 2.7-microns with 90 Å pores
 HALO provides the efficiency of sub-2-micron particles at
half the pressure
 New HALO-5 particles are ~ 5-microns with 90 Å pores
 HALO-5 provides the efficiency of 3-micron particles with
the pressure of 5-micron particles

4. HALO -5 Fused-Core Particles
®
~ 5 µm
0.6 µm
SEM of ~ 5 µm HALO Fused-core Graphical representation of ~ 5 µm HALO Fused-core

6. • As predicted by theory, the smaller the particle, the smaller the plate height
(e.g., the sharper the peaks)
• The 4 µm and 2.2 µm particles are from R&D batches

7. • The reduced plate height, h (same plate height data divided by the particle
size), provides a measure of packed bed quality.
• The larger-particle HALO packings exhibit lower values for h, indicating
more homogeneous packed beds. HALO(2.7) h = 1.5, HALO-5 h = 1.2!

8. More Theoretical Plates at Lower Pressure
• When operated at optimum flow rates, HALO-5 columns provide the
highest number of theoretical plates per unit of pressure

9. So how much does a
theoretical plate weigh?
Based on the previous graph, a theoretical plate from
a column packed with 1.8µ particles weighs 0.025 bar.
A theoretical plate from a column packed with HALO-
5 weighs 0.0028 bar.
Practical significance: You get 10X as many HALO-5
plates per pound!

10. HALO-5 Bonded Phases
Available November 2012
- Phenyl-Hexyl
- C8
- ES-CN
C18 (octadecyl)
Available February 2013
PFP - HILIC
(pentafluorophenylpropyl) -Penta-HILIC

11. Who Should Use HALO-5 Columns?
 Users who currently use 3- or 5-micron
particle columns and want to increase their
efficiency and speed
 Anyone wanting to transfer a method to a
routine laboratory
 Users who want more speed or who want
more separation power but are owners of
pressure-limited HPLC instruments (400 bar
max pressure)

12. Conclusions
Regarding HALO-5 Columns
 Produce unusual column efficiency; reduced plate
heights h of ~1.2 for small molecules making them the
first high-performance 5-micron columns in the world.
 Efficiency and separation speed rivals that of
3-μm columns, but with about one-half the pressure.
 Thin porous outer shell results in superior mass
transfer kinetics and better efficiency at high mobile
phase velocities, especially for larger molecules.
 2-μm porosity frits allow the convenience and
ruggedness of columns with 5 μm particles.
 Strong particles allow pressures of at least 600 bar.

13. The line it is drawn, the curse it is cast
The slow one now will later be fast
As the present now will later be past
The order is rapidly fadin’
And the first one now will later be last
For the times they are a-changin’
Bob Dylan 1964

14. Easy Transfer from Existing Methods
• HALO-5 Columns Can be Substituted for 5-Micron Totally
Porous Columns to Provide Sharper Peaks and Shorter Analysis
Times Under Identical Method Parameters

15. Obtain 5-Micron Benefits for 3-Micron Methods
• HALO-5 Columns Provide the Same Efficiency as 3-Micron
Columns at about Half the Pressure

16. Comparable Selectivity with 2.7-Micron HALO
PEAK IDENTITIES (in order):
1. Uracil
2. Resorcinol
3. Aniline
4. 4-Chloroaniline
5. Acetoacetanilide
6. Dimethylphthalate
7. Cinnamyl alcohol
8. 2,6-Dinitrotoluene
9. Tolbutamide
10. 4-Chloro-3-nitroanisole
• Methods are easily transferred from HALO 2.7-micron
columns to HALO-5 Columns with only minor adjustment
to mobile phase strength

17. HALO-5 Columns Exhibit the Same Sample Loadability as 2.7-Micron
HALO Columns and are Similar to 5-Micron Totally Porous Columns
35000
30000
25000
2.7 µm Fused-Core, 0.5 µm shell
20000
5 µm Fused-Core, 0.6 µm shell
5 µm Totally porous
15000
m
N
b
u
P
e
a
r
t
l
10000
5000
0.01 0.1 1 10 100
µg of 1-Cl-4-Nitrobenzene

18. Back Pressure Plots for Columns of Different
Particle Sizes
600
2.7 µm Halo fused-core
3 µm totally porous
4 µm Halo fused-core
500
5 µm Halo fused-core
5 µm totally porous
Linear regression
400
Pressure, bar
300
200
•Columns: 150 x 4.6 mm, C18
•Mobile Phase:
50% ACN, 50% Water
100
•Temperature: 30°C
•Instruments: Agilent 1100 or
Agilent 1200 SL
0
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0
Mobile Phase Flow Rate, mL/min