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Semester: 5
Instructor: Saira Khalid
2
1. Analytical Chemistry by Gary D. Christian
2. Fundamentals of Analytical Chemistry by Skoog,
West and Holler
3. Quantitative Chemical Analysis by Daniel C. Harris
4. Organic Spectroscopy by M. Younus
Spectroscopy
UV/Visible Spectroscopy
IR Spectroscopy
The branch of science concerned with the substances of
which matter is composed, the investigation of their
properties and reactions, and the use of such reactions to
form new substances.
4
B.P = 100 oC
Color = Colorless
1 molecule of Oxygen
2 molecules of Hydrogen
Bonding= Hydrogen
Bonding
5

6
Chemistry in Everyday Life
Body Composition
Coffee Keeps us Awake
Adenosine
Chemistry of an Onion
Chemistry of Photosynthesis
volatile gas
propanethiol S-oxide
Chemistry in Baking

7
Analytical Chemistry
Analytical chemistry studies and uses instruments and
methods used to separate, identify, and quantify matter.
Instruments Methods
 Titrators
 Spectrophotometers
 Chromatogram
 Potentiometers
 Electron Microscopy
 Titrimetric method
 Spectroscopic method
 Separation method
 Electrochemical
analysis
 Microscopic method
8
Scope of Analytical Chemistry
9


The interactions of radiations and matter are the subject of
spectroscopy or spectrochemical methods (also called
spectrometry).
 Spectrochemical methods usually measure the
electromagnetic radiation produced (emitted) or absorbed
by molecular or atomic species of interest.
 It has to be added though that spectroscopy nowadays
includes some methods that do not involve EM radiation,
such as acoustic and mass spectroscopy.

Introduction to
Spectrochemical Methods
Spectroscopic methods are among the most
selective and sensitive analytical methods,
and therefore are the second most widely
used methods (after chromatography).
Spectroscopy also played an important role
in the development of atomic theory.

 Light consists of electromagnetic radiations EMR.
 EMR travels in the form of a wave.

Wave nature of Light
 All EMR are associated with oscillating electric as
well as magnetic fields at right angle to each other
and also at right angle to direction of propagation.

Wavelength is the distance between two successive crests or
trough of a wave, especially points in a sound wave or
electromagnetic wave.

Frequency
The number of waves that pass a fixed point in unit time
or the Frequency of EM wave is the number of
oscillations that occur in one second.

Calculate the frequency of
wavelength of 400nm.

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1st lecture analytical chemistry

  • 2. 2 1. Analytical Chemistry by Gary D. Christian 2. Fundamentals of Analytical Chemistry by Skoog, West and Holler 3. Quantitative Chemical Analysis by Daniel C. Harris 4. Organic Spectroscopy by M. Younus
  • 4. The branch of science concerned with the substances of which matter is composed, the investigation of their properties and reactions, and the use of such reactions to form new substances. 4 B.P = 100 oC Color = Colorless 1 molecule of Oxygen 2 molecules of Hydrogen Bonding= Hydrogen Bonding
  • 5. 5
  • 6.  6 Chemistry in Everyday Life Body Composition Coffee Keeps us Awake Adenosine Chemistry of an Onion Chemistry of Photosynthesis volatile gas propanethiol S-oxide Chemistry in Baking
  • 7.  7 Analytical Chemistry Analytical chemistry studies and uses instruments and methods used to separate, identify, and quantify matter. Instruments Methods  Titrators  Spectrophotometers  Chromatogram  Potentiometers  Electron Microscopy  Titrimetric method  Spectroscopic method  Separation method  Electrochemical analysis  Microscopic method
  • 9. 9
  • 10.   The interactions of radiations and matter are the subject of spectroscopy or spectrochemical methods (also called spectrometry).  Spectrochemical methods usually measure the electromagnetic radiation produced (emitted) or absorbed by molecular or atomic species of interest.  It has to be added though that spectroscopy nowadays includes some methods that do not involve EM radiation, such as acoustic and mass spectroscopy.  Introduction to Spectrochemical Methods
  • 11. Spectroscopic methods are among the most selective and sensitive analytical methods, and therefore are the second most widely used methods (after chromatography). Spectroscopy also played an important role in the development of atomic theory.
  • 12.   Light consists of electromagnetic radiations EMR.  EMR travels in the form of a wave.
  • 13.  Wave nature of Light  All EMR are associated with oscillating electric as well as magnetic fields at right angle to each other and also at right angle to direction of propagation.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18.  Wavelength is the distance between two successive crests or trough of a wave, especially points in a sound wave or electromagnetic wave.
  • 19.
  • 20.  Frequency The number of waves that pass a fixed point in unit time or the Frequency of EM wave is the number of oscillations that occur in one second.
  • 21.
  • 22.
  • 23.  Calculate the frequency of wavelength of 400nm.