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1) YELLOW FEVER:
Group: Group IV ((+) ssRNA)
Order: Unassigned
Family: Flaviviridae
Genus: Flavivirus
Species: Yellow fever virus
CAUSATIVE AGENT: Yellow fever is caused by the yellow fever virus, a 40- to 50-nm-wide
enveloped RNA virus, the type species and namesake of the family Flaviviridae. It was the first
illness shown to be transmissible by filtered human serum and transmitted by mosquitoes.
VECTOR: Yellow fever virus is mainly transmitted through the bite of the yellow fever
mosquito Aedes aegypti, but other mostly Aedes mosquitoes such as the tiger mosquito (Aedes
albopictus) can also serve as a vector for this virus.
CHIKUNGUNYA:
Chikungunya virus is transmitted to people through mosquito bites. Mosquitoes become infected
when they feed on a person already infected with the virus. Infected mosquitoes can then spread
the virus to other people through bites.
Chikungunya virus is most often spread to people by Aedes aegypti and Aedes albopictus
mosquitoes. These are the same mosquitoes that transmit dengue virus. They bite during the day
and at night.
In the continental United States, vector control professionals use integrated vector management
strategies to control Aedes aegypti and Aedes albopictus mosquitoes.
Before a locally-acquired case(s) of chikungunya infection occurs, professionals conduct
surveillance to understand local populations of mosquitoes and begin control efforts.
If a travel-related case is reported or locally-acquired case is suspected, vector control
professionals enhance mosquito control activities to reduce both larvae and adult mosquito
densities. This can help keep mosquitoes from biting infected people, which can break the
transmission cycle.
Vector surveillance and control efforts should target mosquito species that can transmit viruses.
Control activities are generally similar for Aedes aegypti and Aedes albopictus mosquitoes.
IS IT POSSSIBLE THAT CHICKUNGUNYA and YELLOW FEVER COULD BECOME
ESTABLISHED IN THE FUTURE IN UNITED STATES:
Yes. Since December 2013, the chikungunya and yellow fever viruses have spread to many new
countries and territories in the Americas (the Caribbean, Central, Latin, North and South
America) and infected increasing numbers of people.
As long as the these epidemic continues, travelers may become infected and spread the virus.
The mosquitoes that can transmit chikungunya virus are common in many parts of the Americas,
including parts of the United States. In these locations, travelers infected with chikungunya virus
may be bitten by mosquitoes after returning home, which can lead to local cases or outbreaks.
Each year, millions of travelers visit countries where chikungunya outbreaks are ongoing. People
become infected through mosquito bites. The two types of mosquitoes that can spread
chikungunya virus - Aedes aegypti and Aedes albopictus - are found in parts of the U.S. so it is
possible for the virus to spread here once imported.
Infected travelers bring these viruses into the U.S. every year. From 20062013, an average of 28
people per year had confirmed cases of chikungunya. All were travelers visiting or returning to
the United States from affected areas, mostly in Asia. None of those imported cases resulted in
locally-acquired cases or an outbreak.
However, more chikungunya-infected travelers will come into the U.S. from the Americas,
increasing the likelihood that limited local chikungunya virus transmission could occur. Since
the Caribbean outbreak began in December, 2013, over 750 travelers have returned to the U.S.
infected with chikungunya virus. And as of August 2013, a handful of locally acquired cases had
been reported in the continental U.S. It is important for public health experts and healthcare
providers to be aware of chikungunya in patients with a recent travel history and to test for and
report cases.
2) Influenza Virus are remarkable because of the frequent antigenic change that occurs in HA
(hemagglutinin) or NA (neuraminidase). The two surface antigens of influenza undergo antigenic
variation independent of each other. They are Antigenic Shift and Antigenic Drift.
Some of the Differences Between Antigenic Shift and Antigenic Drift are as follows:
S.N.
Antigenic Shift
Antigenic Drift
1
Major Antigenic Change
Minor Antigenic Change
2
Forming new sub-type (Subtype A + Subtype B –> New Subtype)
Forming new strain of virus
3
One or Two Viruses are Involved
Only one virus is involve
4
Occurs once in a time
Occurs frequently
5
May jump from one species to another (animal-human)
May infect animals of the same species
6
Large change in nucleotides of RNA
Small mutation of RNA
7
Occurs as a results of genome reassortment between difference subtypes.
Occurs as a result of the accumulation of point mutations in the gene.
8
An antigenic change which results in drastic or dramatic alternation in HA (hemagglutinin) or
NA (neuraminidase) subtypes.
An antigenic change can alter antigenic sites on the molecule such that a virion can escape
recognition by the host’s immune system.
9
Large and sudden mutation
Random and Spontaneous Mutation
10
Difficult to treat (need new vaccine)
Easy to treat (antibody and drugs available)
11
Occurs only in Influenza Virus A
Occurs in Influenza Virus A, B and C
12
Give rise to pandemics, which occurs irregularly and unpredictably.
Usually responsible for epidemics in between pandemics.
13
Example: The 1968 pandemic arose when the H3 hemagglutinin gene and one other internal
gene from an avian donor reassorted with the N2 neuraminidase and five other genes from the
H2N2 human strain that had been in circulation.
Example: The 1918 pandemic arose when an avian H1N1 strain mutated to enable its rapid and
efficient transfer from human-to-human.
Example: The subtle mutations accumulated through antigenic drift of these subtypes (e.g.,
H1N1, H3N2, H5N1) give rise to different strains of each subtype.
Example: Antigenic drift is also known to occur in HIV (human immunodeficiency virus),
which causes AIDS, and in certain rhinoviruses, which cause common colds in humans. It also
has been suspected to occur in some cancer-causing viruses in humans.
VACCINATION and ANTIGENIC DRIFT IN INFLUENZA:
The relationship between influenza antigenic drift and vaccination lies at the intersection of
evolutionary biology and public health, and it must be viewed and analyzed in both contexts
simultaneously.
If antigenic drift occurs on the time scale of a single influenza season, it may be associated with
the presence of herd immunity at the beginning of the season and may indicate a need to monitor
for vaccine updates at the end of the season. The relationship between antigenic drift and
vaccination must also be viewed in the context of the global circulation of influenza strains and
the seeding of local and regional epidemics. In the data sets considered from New Zealand, New
York, and France show that antigenic drift can be statistically detected during some seasons, and
seeding of epidemics appears to be endogenous sometimes and exogenous at other times.
Improved detection of short-term antigenic drift and epidemic seeding would significantly
benefit influenza monitoring efforts and vaccine selection.
S.N.
Antigenic Shift
Antigenic Drift
1
Major Antigenic Change
Minor Antigenic Change
2
Forming new sub-type (Subtype A + Subtype B –> New Subtype)
Forming new strain of virus
3
One or Two Viruses are Involved
Only one virus is involve
4
Occurs once in a time
Occurs frequently
5
May jump from one species to another (animal-human)
May infect animals of the same species
6
Large change in nucleotides of RNA
Small mutation of RNA
7
Occurs as a results of genome reassortment between difference subtypes.
Occurs as a result of the accumulation of point mutations in the gene.
8
An antigenic change which results in drastic or dramatic alternation in HA (hemagglutinin) or
NA (neuraminidase) subtypes.
An antigenic change can alter antigenic sites on the molecule such that a virion can escape
recognition by the host’s immune system.
9
Large and sudden mutation
Random and Spontaneous Mutation
10
Difficult to treat (need new vaccine)
Easy to treat (antibody and drugs available)
11
Occurs only in Influenza Virus A
Occurs in Influenza Virus A, B and C
12
Give rise to pandemics, which occurs irregularly and unpredictably.
Usually responsible for epidemics in between pandemics.
13
Example: The 1968 pandemic arose when the H3 hemagglutinin gene and one other internal
gene from an avian donor reassorted with the N2 neuraminidase and five other genes from the
H2N2 human strain that had been in circulation.
Example: The 1918 pandemic arose when an avian H1N1 strain mutated to enable its rapid and
efficient transfer from human-to-human.
Example: The subtle mutations accumulated through antigenic drift of these subtypes (e.g.,
H1N1, H3N2, H5N1) give rise to different strains of each subtype.
Example: Antigenic drift is also known to occur in HIV (human immunodeficiency virus),
which causes AIDS, and in certain rhinoviruses, which cause common colds in humans. It also
has been suspected to occur in some cancer-causing viruses in humans.
Solution
1) YELLOW FEVER:
Group: Group IV ((+) ssRNA)
Order: Unassigned
Family: Flaviviridae
Genus: Flavivirus
Species: Yellow fever virus
CAUSATIVE AGENT: Yellow fever is caused by the yellow fever virus, a 40- to 50-nm-wide
enveloped RNA virus, the type species and namesake of the family Flaviviridae. It was the first
illness shown to be transmissible by filtered human serum and transmitted by mosquitoes.
VECTOR: Yellow fever virus is mainly transmitted through the bite of the yellow fever
mosquito Aedes aegypti, but other mostly Aedes mosquitoes such as the tiger mosquito (Aedes
albopictus) can also serve as a vector for this virus.
CHIKUNGUNYA:
Chikungunya virus is transmitted to people through mosquito bites. Mosquitoes become infected
when they feed on a person already infected with the virus. Infected mosquitoes can then spread
the virus to other people through bites.
Chikungunya virus is most often spread to people by Aedes aegypti and Aedes albopictus
mosquitoes. These are the same mosquitoes that transmit dengue virus. They bite during the day
and at night.
In the continental United States, vector control professionals use integrated vector management
strategies to control Aedes aegypti and Aedes albopictus mosquitoes.
Before a locally-acquired case(s) of chikungunya infection occurs, professionals conduct
surveillance to understand local populations of mosquitoes and begin control efforts.
If a travel-related case is reported or locally-acquired case is suspected, vector control
professionals enhance mosquito control activities to reduce both larvae and adult mosquito
densities. This can help keep mosquitoes from biting infected people, which can break the
transmission cycle.
Vector surveillance and control efforts should target mosquito species that can transmit viruses.
Control activities are generally similar for Aedes aegypti and Aedes albopictus mosquitoes.
IS IT POSSSIBLE THAT CHICKUNGUNYA and YELLOW FEVER COULD BECOME
ESTABLISHED IN THE FUTURE IN UNITED STATES:
Yes. Since December 2013, the chikungunya and yellow fever viruses have spread to many new
countries and territories in the Americas (the Caribbean, Central, Latin, North and South
America) and infected increasing numbers of people.
As long as the these epidemic continues, travelers may become infected and spread the virus.
The mosquitoes that can transmit chikungunya virus are common in many parts of the Americas,
including parts of the United States. In these locations, travelers infected with chikungunya virus
may be bitten by mosquitoes after returning home, which can lead to local cases or outbreaks.
Each year, millions of travelers visit countries where chikungunya outbreaks are ongoing. People
become infected through mosquito bites. The two types of mosquitoes that can spread
chikungunya virus - Aedes aegypti and Aedes albopictus - are found in parts of the U.S. so it is
possible for the virus to spread here once imported.
Infected travelers bring these viruses into the U.S. every year. From 20062013, an average of 28
people per year had confirmed cases of chikungunya. All were travelers visiting or returning to
the United States from affected areas, mostly in Asia. None of those imported cases resulted in
locally-acquired cases or an outbreak.
However, more chikungunya-infected travelers will come into the U.S. from the Americas,
increasing the likelihood that limited local chikungunya virus transmission could occur. Since
the Caribbean outbreak began in December, 2013, over 750 travelers have returned to the U.S.
infected with chikungunya virus. And as of August 2013, a handful of locally acquired cases had
been reported in the continental U.S. It is important for public health experts and healthcare
providers to be aware of chikungunya in patients with a recent travel history and to test for and
report cases.
2) Influenza Virus are remarkable because of the frequent antigenic change that occurs in HA
(hemagglutinin) or NA (neuraminidase). The two surface antigens of influenza undergo antigenic
variation independent of each other. They are Antigenic Shift and Antigenic Drift.
Some of the Differences Between Antigenic Shift and Antigenic Drift are as follows:
S.N.
Antigenic Shift
Antigenic Drift
1
Major Antigenic Change
Minor Antigenic Change
2
Forming new sub-type (Subtype A + Subtype B –> New Subtype)
Forming new strain of virus
3
One or Two Viruses are Involved
Only one virus is involve
4
Occurs once in a time
Occurs frequently
5
May jump from one species to another (animal-human)
May infect animals of the same species
6
Large change in nucleotides of RNA
Small mutation of RNA
7
Occurs as a results of genome reassortment between difference subtypes.
Occurs as a result of the accumulation of point mutations in the gene.
8
An antigenic change which results in drastic or dramatic alternation in HA (hemagglutinin) or
NA (neuraminidase) subtypes.
An antigenic change can alter antigenic sites on the molecule such that a virion can escape
recognition by the host’s immune system.
9
Large and sudden mutation
Random and Spontaneous Mutation
10
Difficult to treat (need new vaccine)
Easy to treat (antibody and drugs available)
11
Occurs only in Influenza Virus A
Occurs in Influenza Virus A, B and C
12
Give rise to pandemics, which occurs irregularly and unpredictably.
Usually responsible for epidemics in between pandemics.
13
Example: The 1968 pandemic arose when the H3 hemagglutinin gene and one other internal
gene from an avian donor reassorted with the N2 neuraminidase and five other genes from the
H2N2 human strain that had been in circulation.
Example: The 1918 pandemic arose when an avian H1N1 strain mutated to enable its rapid and
efficient transfer from human-to-human.
Example: The subtle mutations accumulated through antigenic drift of these subtypes (e.g.,
H1N1, H3N2, H5N1) give rise to different strains of each subtype.
Example: Antigenic drift is also known to occur in HIV (human immunodeficiency virus),
which causes AIDS, and in certain rhinoviruses, which cause common colds in humans. It also
has been suspected to occur in some cancer-causing viruses in humans.
VACCINATION and ANTIGENIC DRIFT IN INFLUENZA:
The relationship between influenza antigenic drift and vaccination lies at the intersection of
evolutionary biology and public health, and it must be viewed and analyzed in both contexts
simultaneously.
If antigenic drift occurs on the time scale of a single influenza season, it may be associated with
the presence of herd immunity at the beginning of the season and may indicate a need to monitor
for vaccine updates at the end of the season. The relationship between antigenic drift and
vaccination must also be viewed in the context of the global circulation of influenza strains and
the seeding of local and regional epidemics. In the data sets considered from New Zealand, New
York, and France show that antigenic drift can be statistically detected during some seasons, and
seeding of epidemics appears to be endogenous sometimes and exogenous at other times.
Improved detection of short-term antigenic drift and epidemic seeding would significantly
benefit influenza monitoring efforts and vaccine selection.
S.N.
Antigenic Shift
Antigenic Drift
1
Major Antigenic Change
Minor Antigenic Change
2
Forming new sub-type (Subtype A + Subtype B –> New Subtype)
Forming new strain of virus
3
One or Two Viruses are Involved
Only one virus is involve
4
Occurs once in a time
Occurs frequently
5
May jump from one species to another (animal-human)
May infect animals of the same species
6
Large change in nucleotides of RNA
Small mutation of RNA
7
Occurs as a results of genome reassortment between difference subtypes.
Occurs as a result of the accumulation of point mutations in the gene.
8
An antigenic change which results in drastic or dramatic alternation in HA (hemagglutinin) or
NA (neuraminidase) subtypes.
An antigenic change can alter antigenic sites on the molecule such that a virion can escape
recognition by the host’s immune system.
9
Large and sudden mutation
Random and Spontaneous Mutation
10
Difficult to treat (need new vaccine)
Easy to treat (antibody and drugs available)
11
Occurs only in Influenza Virus A
Occurs in Influenza Virus A, B and C
12
Give rise to pandemics, which occurs irregularly and unpredictably.
Usually responsible for epidemics in between pandemics.
13
Example: The 1968 pandemic arose when the H3 hemagglutinin gene and one other internal
gene from an avian donor reassorted with the N2 neuraminidase and five other genes from the
H2N2 human strain that had been in circulation.
Example: The 1918 pandemic arose when an avian H1N1 strain mutated to enable its rapid and
efficient transfer from human-to-human.
Example: The subtle mutations accumulated through antigenic drift of these subtypes (e.g.,
H1N1, H3N2, H5N1) give rise to different strains of each subtype.
Example: Antigenic drift is also known to occur in HIV (human immunodeficiency virus),
which causes AIDS, and in certain rhinoviruses, which cause common colds in humans. It also
has been suspected to occur in some cancer-causing viruses in humans.

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1) YELLOW FEVERGroup Group IV ((+) ssRNA)Order   Unassigned.pdf

  • 1. 1) YELLOW FEVER: Group: Group IV ((+) ssRNA) Order: Unassigned Family: Flaviviridae Genus: Flavivirus Species: Yellow fever virus CAUSATIVE AGENT: Yellow fever is caused by the yellow fever virus, a 40- to 50-nm-wide enveloped RNA virus, the type species and namesake of the family Flaviviridae. It was the first illness shown to be transmissible by filtered human serum and transmitted by mosquitoes. VECTOR: Yellow fever virus is mainly transmitted through the bite of the yellow fever mosquito Aedes aegypti, but other mostly Aedes mosquitoes such as the tiger mosquito (Aedes albopictus) can also serve as a vector for this virus. CHIKUNGUNYA: Chikungunya virus is transmitted to people through mosquito bites. Mosquitoes become infected when they feed on a person already infected with the virus. Infected mosquitoes can then spread the virus to other people through bites. Chikungunya virus is most often spread to people by Aedes aegypti and Aedes albopictus mosquitoes. These are the same mosquitoes that transmit dengue virus. They bite during the day and at night. In the continental United States, vector control professionals use integrated vector management strategies to control Aedes aegypti and Aedes albopictus mosquitoes. Before a locally-acquired case(s) of chikungunya infection occurs, professionals conduct surveillance to understand local populations of mosquitoes and begin control efforts. If a travel-related case is reported or locally-acquired case is suspected, vector control professionals enhance mosquito control activities to reduce both larvae and adult mosquito densities. This can help keep mosquitoes from biting infected people, which can break the transmission cycle. Vector surveillance and control efforts should target mosquito species that can transmit viruses. Control activities are generally similar for Aedes aegypti and Aedes albopictus mosquitoes. IS IT POSSSIBLE THAT CHICKUNGUNYA and YELLOW FEVER COULD BECOME ESTABLISHED IN THE FUTURE IN UNITED STATES: Yes. Since December 2013, the chikungunya and yellow fever viruses have spread to many new countries and territories in the Americas (the Caribbean, Central, Latin, North and South America) and infected increasing numbers of people. As long as the these epidemic continues, travelers may become infected and spread the virus.
  • 2. The mosquitoes that can transmit chikungunya virus are common in many parts of the Americas, including parts of the United States. In these locations, travelers infected with chikungunya virus may be bitten by mosquitoes after returning home, which can lead to local cases or outbreaks. Each year, millions of travelers visit countries where chikungunya outbreaks are ongoing. People become infected through mosquito bites. The two types of mosquitoes that can spread chikungunya virus - Aedes aegypti and Aedes albopictus - are found in parts of the U.S. so it is possible for the virus to spread here once imported. Infected travelers bring these viruses into the U.S. every year. From 20062013, an average of 28 people per year had confirmed cases of chikungunya. All were travelers visiting or returning to the United States from affected areas, mostly in Asia. None of those imported cases resulted in locally-acquired cases or an outbreak. However, more chikungunya-infected travelers will come into the U.S. from the Americas, increasing the likelihood that limited local chikungunya virus transmission could occur. Since the Caribbean outbreak began in December, 2013, over 750 travelers have returned to the U.S. infected with chikungunya virus. And as of August 2013, a handful of locally acquired cases had been reported in the continental U.S. It is important for public health experts and healthcare providers to be aware of chikungunya in patients with a recent travel history and to test for and report cases. 2) Influenza Virus are remarkable because of the frequent antigenic change that occurs in HA (hemagglutinin) or NA (neuraminidase). The two surface antigens of influenza undergo antigenic variation independent of each other. They are Antigenic Shift and Antigenic Drift. Some of the Differences Between Antigenic Shift and Antigenic Drift are as follows: S.N. Antigenic Shift Antigenic Drift 1 Major Antigenic Change Minor Antigenic Change 2 Forming new sub-type (Subtype A + Subtype B –> New Subtype) Forming new strain of virus 3 One or Two Viruses are Involved Only one virus is involve 4
  • 3. Occurs once in a time Occurs frequently 5 May jump from one species to another (animal-human) May infect animals of the same species 6 Large change in nucleotides of RNA Small mutation of RNA 7 Occurs as a results of genome reassortment between difference subtypes. Occurs as a result of the accumulation of point mutations in the gene. 8 An antigenic change which results in drastic or dramatic alternation in HA (hemagglutinin) or NA (neuraminidase) subtypes. An antigenic change can alter antigenic sites on the molecule such that a virion can escape recognition by the host’s immune system. 9 Large and sudden mutation Random and Spontaneous Mutation 10 Difficult to treat (need new vaccine) Easy to treat (antibody and drugs available) 11 Occurs only in Influenza Virus A Occurs in Influenza Virus A, B and C 12 Give rise to pandemics, which occurs irregularly and unpredictably. Usually responsible for epidemics in between pandemics. 13 Example: The 1968 pandemic arose when the H3 hemagglutinin gene and one other internal gene from an avian donor reassorted with the N2 neuraminidase and five other genes from the H2N2 human strain that had been in circulation. Example: The 1918 pandemic arose when an avian H1N1 strain mutated to enable its rapid and efficient transfer from human-to-human. Example: The subtle mutations accumulated through antigenic drift of these subtypes (e.g., H1N1, H3N2, H5N1) give rise to different strains of each subtype.
  • 4. Example: Antigenic drift is also known to occur in HIV (human immunodeficiency virus), which causes AIDS, and in certain rhinoviruses, which cause common colds in humans. It also has been suspected to occur in some cancer-causing viruses in humans. VACCINATION and ANTIGENIC DRIFT IN INFLUENZA: The relationship between influenza antigenic drift and vaccination lies at the intersection of evolutionary biology and public health, and it must be viewed and analyzed in both contexts simultaneously. If antigenic drift occurs on the time scale of a single influenza season, it may be associated with the presence of herd immunity at the beginning of the season and may indicate a need to monitor for vaccine updates at the end of the season. The relationship between antigenic drift and vaccination must also be viewed in the context of the global circulation of influenza strains and the seeding of local and regional epidemics. In the data sets considered from New Zealand, New York, and France show that antigenic drift can be statistically detected during some seasons, and seeding of epidemics appears to be endogenous sometimes and exogenous at other times. Improved detection of short-term antigenic drift and epidemic seeding would significantly benefit influenza monitoring efforts and vaccine selection. S.N. Antigenic Shift Antigenic Drift 1 Major Antigenic Change Minor Antigenic Change 2 Forming new sub-type (Subtype A + Subtype B –> New Subtype) Forming new strain of virus 3 One or Two Viruses are Involved Only one virus is involve 4 Occurs once in a time Occurs frequently 5 May jump from one species to another (animal-human) May infect animals of the same species 6 Large change in nucleotides of RNA
  • 5. Small mutation of RNA 7 Occurs as a results of genome reassortment between difference subtypes. Occurs as a result of the accumulation of point mutations in the gene. 8 An antigenic change which results in drastic or dramatic alternation in HA (hemagglutinin) or NA (neuraminidase) subtypes. An antigenic change can alter antigenic sites on the molecule such that a virion can escape recognition by the host’s immune system. 9 Large and sudden mutation Random and Spontaneous Mutation 10 Difficult to treat (need new vaccine) Easy to treat (antibody and drugs available) 11 Occurs only in Influenza Virus A Occurs in Influenza Virus A, B and C 12 Give rise to pandemics, which occurs irregularly and unpredictably. Usually responsible for epidemics in between pandemics. 13 Example: The 1968 pandemic arose when the H3 hemagglutinin gene and one other internal gene from an avian donor reassorted with the N2 neuraminidase and five other genes from the H2N2 human strain that had been in circulation. Example: The 1918 pandemic arose when an avian H1N1 strain mutated to enable its rapid and efficient transfer from human-to-human. Example: The subtle mutations accumulated through antigenic drift of these subtypes (e.g., H1N1, H3N2, H5N1) give rise to different strains of each subtype. Example: Antigenic drift is also known to occur in HIV (human immunodeficiency virus), which causes AIDS, and in certain rhinoviruses, which cause common colds in humans. It also has been suspected to occur in some cancer-causing viruses in humans. Solution 1) YELLOW FEVER:
  • 6. Group: Group IV ((+) ssRNA) Order: Unassigned Family: Flaviviridae Genus: Flavivirus Species: Yellow fever virus CAUSATIVE AGENT: Yellow fever is caused by the yellow fever virus, a 40- to 50-nm-wide enveloped RNA virus, the type species and namesake of the family Flaviviridae. It was the first illness shown to be transmissible by filtered human serum and transmitted by mosquitoes. VECTOR: Yellow fever virus is mainly transmitted through the bite of the yellow fever mosquito Aedes aegypti, but other mostly Aedes mosquitoes such as the tiger mosquito (Aedes albopictus) can also serve as a vector for this virus. CHIKUNGUNYA: Chikungunya virus is transmitted to people through mosquito bites. Mosquitoes become infected when they feed on a person already infected with the virus. Infected mosquitoes can then spread the virus to other people through bites. Chikungunya virus is most often spread to people by Aedes aegypti and Aedes albopictus mosquitoes. These are the same mosquitoes that transmit dengue virus. They bite during the day and at night. In the continental United States, vector control professionals use integrated vector management strategies to control Aedes aegypti and Aedes albopictus mosquitoes. Before a locally-acquired case(s) of chikungunya infection occurs, professionals conduct surveillance to understand local populations of mosquitoes and begin control efforts. If a travel-related case is reported or locally-acquired case is suspected, vector control professionals enhance mosquito control activities to reduce both larvae and adult mosquito densities. This can help keep mosquitoes from biting infected people, which can break the transmission cycle. Vector surveillance and control efforts should target mosquito species that can transmit viruses. Control activities are generally similar for Aedes aegypti and Aedes albopictus mosquitoes. IS IT POSSSIBLE THAT CHICKUNGUNYA and YELLOW FEVER COULD BECOME ESTABLISHED IN THE FUTURE IN UNITED STATES: Yes. Since December 2013, the chikungunya and yellow fever viruses have spread to many new countries and territories in the Americas (the Caribbean, Central, Latin, North and South America) and infected increasing numbers of people. As long as the these epidemic continues, travelers may become infected and spread the virus. The mosquitoes that can transmit chikungunya virus are common in many parts of the Americas, including parts of the United States. In these locations, travelers infected with chikungunya virus
  • 7. may be bitten by mosquitoes after returning home, which can lead to local cases or outbreaks. Each year, millions of travelers visit countries where chikungunya outbreaks are ongoing. People become infected through mosquito bites. The two types of mosquitoes that can spread chikungunya virus - Aedes aegypti and Aedes albopictus - are found in parts of the U.S. so it is possible for the virus to spread here once imported. Infected travelers bring these viruses into the U.S. every year. From 20062013, an average of 28 people per year had confirmed cases of chikungunya. All were travelers visiting or returning to the United States from affected areas, mostly in Asia. None of those imported cases resulted in locally-acquired cases or an outbreak. However, more chikungunya-infected travelers will come into the U.S. from the Americas, increasing the likelihood that limited local chikungunya virus transmission could occur. Since the Caribbean outbreak began in December, 2013, over 750 travelers have returned to the U.S. infected with chikungunya virus. And as of August 2013, a handful of locally acquired cases had been reported in the continental U.S. It is important for public health experts and healthcare providers to be aware of chikungunya in patients with a recent travel history and to test for and report cases. 2) Influenza Virus are remarkable because of the frequent antigenic change that occurs in HA (hemagglutinin) or NA (neuraminidase). The two surface antigens of influenza undergo antigenic variation independent of each other. They are Antigenic Shift and Antigenic Drift. Some of the Differences Between Antigenic Shift and Antigenic Drift are as follows: S.N. Antigenic Shift Antigenic Drift 1 Major Antigenic Change Minor Antigenic Change 2 Forming new sub-type (Subtype A + Subtype B –> New Subtype) Forming new strain of virus 3 One or Two Viruses are Involved Only one virus is involve 4 Occurs once in a time Occurs frequently
  • 8. 5 May jump from one species to another (animal-human) May infect animals of the same species 6 Large change in nucleotides of RNA Small mutation of RNA 7 Occurs as a results of genome reassortment between difference subtypes. Occurs as a result of the accumulation of point mutations in the gene. 8 An antigenic change which results in drastic or dramatic alternation in HA (hemagglutinin) or NA (neuraminidase) subtypes. An antigenic change can alter antigenic sites on the molecule such that a virion can escape recognition by the host’s immune system. 9 Large and sudden mutation Random and Spontaneous Mutation 10 Difficult to treat (need new vaccine) Easy to treat (antibody and drugs available) 11 Occurs only in Influenza Virus A Occurs in Influenza Virus A, B and C 12 Give rise to pandemics, which occurs irregularly and unpredictably. Usually responsible for epidemics in between pandemics. 13 Example: The 1968 pandemic arose when the H3 hemagglutinin gene and one other internal gene from an avian donor reassorted with the N2 neuraminidase and five other genes from the H2N2 human strain that had been in circulation. Example: The 1918 pandemic arose when an avian H1N1 strain mutated to enable its rapid and efficient transfer from human-to-human. Example: The subtle mutations accumulated through antigenic drift of these subtypes (e.g., H1N1, H3N2, H5N1) give rise to different strains of each subtype. Example: Antigenic drift is also known to occur in HIV (human immunodeficiency virus), which causes AIDS, and in certain rhinoviruses, which cause common colds in humans. It also
  • 9. has been suspected to occur in some cancer-causing viruses in humans. VACCINATION and ANTIGENIC DRIFT IN INFLUENZA: The relationship between influenza antigenic drift and vaccination lies at the intersection of evolutionary biology and public health, and it must be viewed and analyzed in both contexts simultaneously. If antigenic drift occurs on the time scale of a single influenza season, it may be associated with the presence of herd immunity at the beginning of the season and may indicate a need to monitor for vaccine updates at the end of the season. The relationship between antigenic drift and vaccination must also be viewed in the context of the global circulation of influenza strains and the seeding of local and regional epidemics. In the data sets considered from New Zealand, New York, and France show that antigenic drift can be statistically detected during some seasons, and seeding of epidemics appears to be endogenous sometimes and exogenous at other times. Improved detection of short-term antigenic drift and epidemic seeding would significantly benefit influenza monitoring efforts and vaccine selection. S.N. Antigenic Shift Antigenic Drift 1 Major Antigenic Change Minor Antigenic Change 2 Forming new sub-type (Subtype A + Subtype B –> New Subtype) Forming new strain of virus 3 One or Two Viruses are Involved Only one virus is involve 4 Occurs once in a time Occurs frequently 5 May jump from one species to another (animal-human) May infect animals of the same species 6 Large change in nucleotides of RNA Small mutation of RNA 7
  • 10. Occurs as a results of genome reassortment between difference subtypes. Occurs as a result of the accumulation of point mutations in the gene. 8 An antigenic change which results in drastic or dramatic alternation in HA (hemagglutinin) or NA (neuraminidase) subtypes. An antigenic change can alter antigenic sites on the molecule such that a virion can escape recognition by the host’s immune system. 9 Large and sudden mutation Random and Spontaneous Mutation 10 Difficult to treat (need new vaccine) Easy to treat (antibody and drugs available) 11 Occurs only in Influenza Virus A Occurs in Influenza Virus A, B and C 12 Give rise to pandemics, which occurs irregularly and unpredictably. Usually responsible for epidemics in between pandemics. 13 Example: The 1968 pandemic arose when the H3 hemagglutinin gene and one other internal gene from an avian donor reassorted with the N2 neuraminidase and five other genes from the H2N2 human strain that had been in circulation. Example: The 1918 pandemic arose when an avian H1N1 strain mutated to enable its rapid and efficient transfer from human-to-human. Example: The subtle mutations accumulated through antigenic drift of these subtypes (e.g., H1N1, H3N2, H5N1) give rise to different strains of each subtype. Example: Antigenic drift is also known to occur in HIV (human immunodeficiency virus), which causes AIDS, and in certain rhinoviruses, which cause common colds in humans. It also has been suspected to occur in some cancer-causing viruses in humans.