053 histologic evidence of spio accumulation in vulnerable plaques
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This study examined the accumulation of superparamagnetic iron oxide (SPIO) nanoparticles in vulnerable atherosclerotic plaques. In vitro experiments demonstrated that human macrophages actively take up fluorescent-labeled SPIO particles over 24 hours in a time- and concentration-dependent manner. In vivo studies in mice and rabbit models of atherosclerosis found SPIO particles in macrophage-rich areas of plaques, with a strong correlation between iron staining and foam cell density. Cytokine treatment increased SPIO uptake in plaques. The results suggest MRI using SPIO has potential for noninvasively assessing monocyte recruitment in vulnerable plaques.
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051 plaque inflam detection in atherosclerotic rabbits
051 plaque inflam detection in atherosclerotic rabbitsSociety for Heart Attack Prevention and Eradication
This document proposes a non-invasive method using SPIO (super paramagnetic iron oxide) nanoparticles to image macrophage infiltration and inflammation in vulnerable atherosclerotic plaques. Rabbits and mice were injected with SPIO, which accumulated in macrophage-rich areas of plaques. MR imaging found SPIO accumulation correlated with macrophage density in plaques. Inflamed plaques in hypercholesterolemic rabbits showed enhanced SPIO signals compared to normal rabbits, demonstrating SPIO-enhanced MRI can identify inflammation in vulnerable plaques.Acc presentation macrophage (1)
This document proposes a non-invasive method using SPIO (super paramagnetic iron oxide) nanoparticles to image macrophage infiltration and inflammation in vulnerable atherosclerotic plaques. Rabbits and mice were injected with SPIO, which accumulated in inflamed plaque areas correlated with macrophage density. SPIO-enhanced MRI then successfully identified these inflamed plaques non-invasively in vivo. This technique could provide a way to detect rupture-prone plaques and better understand plaque vulnerability.
Introducing a novel model
1) Researchers studied a novel method for quantifying and tracking macrophage homing into atherosclerotic plaques using superparamagnetic iron oxide nanoparticles (SPIO).
2) They injected SPIO into ApoE-deficient mice with and without proinflammatory cytokines and found higher iron content and more macrophages in plaques of cytokine-treated mice using histology and mass spectrometry.
3) This demonstrates that SPIO allows both quantitative and qualitative assessment of macrophage infiltration and shows infiltration is enhanced by cytokines, suggesting SPIO-enhanced MRI could noninvasively monitor monocyte recruitment dynamics into plaques over time.
Esc macrophage homing
1) Researchers studied a novel method for quantifying and tracking macrophage homing into atherosclerotic plaques using superparamagnetic iron oxide nanoparticles (SPIO).
2) They injected SPIO into ApoE-deficient mice, some of which also received proinflammatory cytokines, and tracked iron uptake in plaques over time.
3) Both quantitative iron assays and staining showed greater iron and macrophage presence in plaques of cytokine-treated mice, demonstrating SPIO can monitor macrophage recruitment influenced by inflammation. This noninvasive MRI-based method may help study monocyte involvement in atherosclerosis.
Aha2001
1) This study investigated the use of super paramagnetic iron oxide (SPIO) nanoparticles and MRI to detect active inflammation in atherosclerotic plaques.
2) In mouse and rabbit models of atherosclerosis, SPIO accumulated in macrophage-rich areas of plaques and enhanced MRI detected these plaques after SPIO injection compared to baseline.
3) A human clinical trial is currently underway to evaluate if SPIO-enhanced MRI can detect vulnerable carotid plaques by identifying inflammation, using histology as a reference standard.
Detection of vp by mri after spio maziar
1) This study investigated the use of super paramagnetic iron oxide (SPIO) nanoparticles and MRI to detect active inflammation in atherosclerotic plaques.
2) In mouse and rabbit models of atherosclerosis, SPIO accumulated in atherosclerotic lesions and correlated with histological markers of inflammation.
3) MRI of plaques in these animals showed changes after SPIO injection, indicating SPIO can help identify actively inflamed plaques.
4) A current human clinical trial is evaluating if SPIO-enhanced MRI can detect vulnerable carotid plaques by comparing MRI to histology of plaques removed in surgery.
Aha 2001 presentation maziar
1) This study investigated the use of super paramagnetic iron oxide (SPIO) nanoparticles and MRI to detect active inflammation in atherosclerotic plaques.
2) In mouse and rabbit models of atherosclerosis, SPIO accumulated in atherosclerotic lesions and correlated with histological markers of inflammation.
3) MRI of plaques in these animals showed changes after SPIO injection, indicating SPIO can help identify actively inflamed plaques.
4) A current human clinical trial is evaluating if SPIO-enhanced MRI can detect vulnerable carotid plaques by comparing MRI to histology of plaques removed in surgery.
031
1) This study investigated the use of super paramagnetic iron oxide (SPIO) nanoparticles and MRI to detect active inflammation in atherosclerotic plaques.
2) In mouse and rabbit models of atherosclerosis, SPIO accumulated in atherosclerotic lesions and correlated with histological markers of inflammation.
3) MRI of plaques in these animals showed changes after SPIO injection, indicating SPIO may help identify plaques with active inflammation.
4) A current human clinical trial is evaluating SPIO-enhanced MRI for detecting vulnerable carotid plaques by comparing MRI to histology of plaques removed in surgery.
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051 plaque inflam detection in atherosclerotic rabbits
051 plaque inflam detection in atherosclerotic rabbitsSociety for Heart Attack Prevention and Eradication
This document proposes a non-invasive method using SPIO (super paramagnetic iron oxide) nanoparticles to image macrophage infiltration and inflammation in vulnerable atherosclerotic plaques. Rabbits and mice were injected with SPIO, which accumulated in macrophage-rich areas of plaques. MR imaging found SPIO accumulation correlated with macrophage density in plaques. Inflamed plaques in hypercholesterolemic rabbits showed enhanced SPIO signals compared to normal rabbits, demonstrating SPIO-enhanced MRI can identify inflammation in vulnerable plaques.Acc presentation macrophage (1)
This document proposes a non-invasive method using SPIO (super paramagnetic iron oxide) nanoparticles to image macrophage infiltration and inflammation in vulnerable atherosclerotic plaques. Rabbits and mice were injected with SPIO, which accumulated in inflamed plaque areas correlated with macrophage density. SPIO-enhanced MRI then successfully identified these inflamed plaques non-invasively in vivo. This technique could provide a way to detect rupture-prone plaques and better understand plaque vulnerability.
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2) They injected SPIO into ApoE-deficient mice with and without proinflammatory cytokines and found higher iron content and more macrophages in plaques of cytokine-treated mice using histology and mass spectrometry.
3) This demonstrates that SPIO allows both quantitative and qualitative assessment of macrophage infiltration and shows infiltration is enhanced by cytokines, suggesting SPIO-enhanced MRI could noninvasively monitor monocyte recruitment dynamics into plaques over time.
Esc macrophage homing
1) Researchers studied a novel method for quantifying and tracking macrophage homing into atherosclerotic plaques using superparamagnetic iron oxide nanoparticles (SPIO).
2) They injected SPIO into ApoE-deficient mice, some of which also received proinflammatory cytokines, and tracked iron uptake in plaques over time.
3) Both quantitative iron assays and staining showed greater iron and macrophage presence in plaques of cytokine-treated mice, demonstrating SPIO can monitor macrophage recruitment influenced by inflammation. This noninvasive MRI-based method may help study monocyte involvement in atherosclerosis.
Aha2001
1) This study investigated the use of super paramagnetic iron oxide (SPIO) nanoparticles and MRI to detect active inflammation in atherosclerotic plaques.
2) In mouse and rabbit models of atherosclerosis, SPIO accumulated in macrophage-rich areas of plaques and enhanced MRI detected these plaques after SPIO injection compared to baseline.
3) A human clinical trial is currently underway to evaluate if SPIO-enhanced MRI can detect vulnerable carotid plaques by identifying inflammation, using histology as a reference standard.
Detection of vp by mri after spio maziar
1) This study investigated the use of super paramagnetic iron oxide (SPIO) nanoparticles and MRI to detect active inflammation in atherosclerotic plaques.
2) In mouse and rabbit models of atherosclerosis, SPIO accumulated in atherosclerotic lesions and correlated with histological markers of inflammation.
3) MRI of plaques in these animals showed changes after SPIO injection, indicating SPIO can help identify actively inflamed plaques.
4) A current human clinical trial is evaluating if SPIO-enhanced MRI can detect vulnerable carotid plaques by comparing MRI to histology of plaques removed in surgery.
Aha 2001 presentation maziar
1) This study investigated the use of super paramagnetic iron oxide (SPIO) nanoparticles and MRI to detect active inflammation in atherosclerotic plaques.
2) In mouse and rabbit models of atherosclerosis, SPIO accumulated in atherosclerotic lesions and correlated with histological markers of inflammation.
3) MRI of plaques in these animals showed changes after SPIO injection, indicating SPIO can help identify actively inflamed plaques.
4) A current human clinical trial is evaluating if SPIO-enhanced MRI can detect vulnerable carotid plaques by comparing MRI to histology of plaques removed in surgery.
031
1) This study investigated the use of super paramagnetic iron oxide (SPIO) nanoparticles and MRI to detect active inflammation in atherosclerotic plaques.
2) In mouse and rabbit models of atherosclerosis, SPIO accumulated in atherosclerotic lesions and correlated with histological markers of inflammation.
3) MRI of plaques in these animals showed changes after SPIO injection, indicating SPIO may help identify plaques with active inflammation.
4) A current human clinical trial is evaluating SPIO-enhanced MRI for detecting vulnerable carotid plaques by comparing MRI to histology of plaques removed in surgery.
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This document summarizes a study investigating the use of superparamagnetic iron oxide (SPIO) nanoparticles and MRI to detect plaque inflammation. The researchers hypothesized that SPIO would be taken up by macrophages in inflamed plaques and alter MRI signals. They injected SPIO into atherosclerotic and normal rabbits and mice. Histological analysis found SPIO accumulated in areas with many macrophages. MRI detected enhanced signals in inflamed plaques of atherosclerotic rabbits post-SPIO injection. The study demonstrates SPIO-enhanced MRI can non-invasively identify inflamed, rupture-prone atherosclerotic plaques by detecting macrophage infiltration.
Spio mri studies by dr naghavi - amersham oct 2003 - part2
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This document discusses a novel tracer for MRI imaging of macrophage infiltration in atherosclerotic plaque. It summarizes research into lipid-coated superparamagnetic iron oxide nanoparticles (SPIOs) that are phagocytosed by macrophages. The researchers tested various SPIO coatings and sizes to maximize macrophage uptake while minimizing oxidative stress. Lipid-coated SPIOs combined with certain aminoglycans showed the highest uptake and lowest induction of reactive oxygen species. The goal is to develop an MRI contrast agent that can noninvasively image vulnerable, inflamed plaques by detecting macrophage presence.
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2) A toolbar will provide one-click access to VP-related information resources.
3) Members can customize "Personal VP Watch" alerts on new science, patents, and industry news.
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3) NKT cells increase atherosclerosis by recognizing glycolipid antigens, while regulatory T cells and B cells decrease disease by producing anti-inflammatory cytokines like IL-10 and TGF-β.
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Nih ppg 9-021
Assistant:
- Project coordination- Project coordination
- Budget management- Budget management
- Regulatory compliance- Regulatory compliance
- Manuscript/abstract preparation- Manuscript/abstract preparation
- Seminar/meeting organization- Seminar/meeting organization
- Interaction with NIH Program Officer- Interaction with NIH Program Officer
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- Analysis of renal corpuscles and tubules in ephrin knockout mice found no significant differences compared to wildtype mice, except a very small reduction in glomerular size in ephrin-A5 knockout mice.
- The subtle phenotype and infrequent kidney failure in the breeding colony suggest ephrin-A2 and ephrin-A5 play only minor roles in kidney development and function. Redundancy with other ephrin family members may compensate for their loss.
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Spio mri studies by dr naghavi - amersham oct 2003 - part2
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1) The website will include an online library called "Atheroline" with medical literature and multimedia about atherosclerosis and VP.
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II. Early studies show OCT can visualize features of coronary plaques in living patients, such as thin fibrous caps, better than IVUS. This suggests OCT may help identify vulnerable plaques at risk of rupturing.
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II. The double knockout LDL/apoE mice are highlighted as offering improvements in studying clinical complications of atherosclerosis like human heart disease. However, it is unclear how closely they model vulnerable plaques.
III. Questions are raised about how closely the coagulation systems of these animal models resemble humans and whether any model fully captures repeated plaque ruptures and the role of aging in natural history as seen in humans.
076 cardiac magnetic resonance imaging
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- It describes how endothelial dysfunction and reduced arterial elasticity (especially in small arteries) are early signs of vascular damage and predict future cardiovascular events independent of traditional risk factors like age and blood pressure.
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This document summarizes the role of the immune system in atherosclerosis. It finds that:
1) Adaptive immunity plays a major role in atherosclerosis as mice lacking T and B cells develop dramatically less atherosclerosis despite similar cholesterol levels.
2) T cells in lesions recognize oxidized LDL and other antigens, activating macrophages and promoting inflammation.
3) NKT cells increase atherosclerosis by recognizing glycolipid antigens, while regulatory T cells and B cells decrease disease by producing anti-inflammatory cytokines like IL-10 and TGF-β.
4) Protective immunization approaches have shown promise in animal models by stimulating antibody production against oxidized LDL and other antigens to decrease plaque formation.
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- Seminar/meeting organization- Seminar/meeting organization
- Interaction with NIH Program Officer- Interaction with NIH Program Officer
221 images of 3 mice
Atherosclerotic plaque contains iron positive cells such as subendothelial macrophages. Macrophages accumulate in the layers of arteries and contribute to the formation of atherosclerotic plaque. The presence of iron positive macrophages is a characteristic of atherosclerotic plaque buildup in arteries.
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045 how can we identify the plaque at risk of rupture or thrombosis
045 how can we identify the plaque at risk of rupture or thrombosisSociety for Heart Attack Prevention and Eradication
1. Temperature measurements of living carotid plaques showed heterogeneity, with differences over 0.3°C seen in all specimens and over 2°C in some. Hotter plaques correlated with inflammatory cells and thin caps.
2. Plaque pH also showed heterogeneity and inversely correlated with temperature. Lipid-rich plaques were acidic while calcified plaques were alkaline.
3. These measurements of temperature and pH heterogeneity could provide information on plaque vulnerability and prognosis when used along with other imaging techniques.229 propagermanium
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Non invasive detection of vulnerable plaque using spio enhanced mri
Non invasive detection of vulnerable plaque using spio enhanced mriSociety for Heart Attack Prevention and Eradication
This document discusses a study on detecting vulnerable plaque using SPIO enhanced MRI. It begins by defining vulnerable plaque as atherosclerotic plaques that can suddenly form blood clots and lead to heart attacks and strokes. It then discusses different types of vulnerable plaque and how plaque morphology alone may not determine outcomes. The study aims to image both plaque morphology and activity using MRI with SPIO contrast agents that can identify inflammation, leaky blood vessels, and intra-plaque hemorrhage. In vitro experiments show macrophages effectively take up SPIO particles, reducing T2 relaxation time, and produce reactive oxygen species upon phagocytosis. In vivo studies in atherosclerotic mice also show SPIO uptake in arterial walls. The goal is to non-059 can oct catheter see plaque macrophages
- Optical coherence tomography (OCT) is a high-resolution imaging technique analogous to ultrasound that uses near-infrared light instead of sound waves. OCT was originally developed for eye imaging and can now be adapted for catheter-based coronary artery imaging.
- Ex vivo studies have shown that OCT, with its 10-20 micron resolution, can characterize atherosclerotic plaque components like fibrous tissue, lipid pools, and calcification. It can also quantify macrophage content in plaque caps.
- In vivo porcine and human studies demonstrate OCT's feasibility for imaging normal coronary arteries, stents, and plaque types like lipid-rich plaques with higher resolution than IVUS. This suggests OCT may be able to completely replace
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045 how can we identify the plaque at risk of rupture or thrombosis
045 how can we identify the plaque at risk of rupture or thrombosis
Non invasive detection of vulnerable plaque using spio enhanced mri
Non invasive detection of vulnerable plaque using spio enhanced mri
Similar to 053 histologic evidence of spio accumulation in vulnerable plaques
031
1) This study investigated the use of super paramagnetic iron oxide (SPIO) nanoparticles and MRI to detect active inflammation in atherosclerotic plaques.
2) In mouse and rabbit models of atherosclerosis, SPIO accumulated in atherosclerotic lesions and correlated with histological markers of inflammation.
3) MRI of plaques in these animals showed changes after SPIO injection, indicating SPIO can help identify actively inflamed plaques.
4) A current human clinical trial is evaluating if SPIO-enhanced MRI can detect vulnerable carotid plaques by comparing MRI to histology of plaques removed in surgery.
040 center for vulnerable plaque research
This document discusses research on using superparamagnetic iron oxide (SPIO) nanoparticles and ultra-small superparamagnetic iron oxide (USPIO) as MRI contrast agents to detect vulnerable plaque. In vitro and animal studies show that macrophages engulf SPIOs, which then accumulate in inflamed plaques and decrease the MRI signal. Histological analysis confirms iron particle presence correlates with macrophage density. MRI of atherosclerotic rabbits after SPIO injection shows darker aortic walls with many iron particles compared to controls. This non-invasive method may help identify vulnerable plaque.
Dr n's presentation at nano vivo seminar
This document discusses vulnerable plaque detection using superparamagnetic iron oxide (SPIO) nanoparticles and MRI. It describes how SPIOs are taken up by macrophages in atherosclerotic plaques, decreasing the MRI signal and allowing plaque visualization. Studies in mice and rabbits show SPIO uptake correlates with plaque inflammation and macrophage content on histology. MRI of rabbits post-SPIO injection detected more signal decrease in hypercholesterolemic rabbits versus controls, indicating potential to identify vulnerable plaque.
040 center for vulnerable plaque research
This document discusses vulnerable plaque detection using superparamagnetic iron oxide (SPIO) nanoparticles and MRI. It describes how SPIOs are taken up by macrophages in atherosclerotic plaques, decreasing the MRI signal and allowing plaque visualization. Studies in mice and rabbits show SPIO uptake correlates with plaque inflammation and macrophage content on histology. MRI of rabbits post-SPIO injection detected more signal decrease in hypercholesterolemic rabbits compared to controls, indicating potential to identify vulnerable plaque.
052 non invasive high resolution detection
1) The document discusses a study using magnetic resonance imaging (MRI) to detect active atherosclerotic plaque in mice after injection of superparamagnetic iron oxide (SPIO) nanoparticles.
2) The study found that SPIO nanoparticles were taken up by macrophages within inflamed plaques, darkening the plaques on MRI.
3) Histological analysis confirmed iron particle presence in plaques, correlated with macrophage density and plaque inflammation. This non-invasive MRI method after SPIO injection shows promise for detecting vulnerable human plaques.
052 non invasive high resolution detection
1) The document discusses a study using magnetic resonance imaging (MRI) to detect active atherosclerotic plaque in mice after injection of superparamagnetic iron oxide (SPIO) nanoparticles.
2) The study found that SPIO nanoparticles were taken up by macrophages within atherosclerotic plaques, darkening the plaques on post-injection MRI scans.
3) Histological analysis confirmed iron particle presence within plaques that correlated with macrophage density, supporting MRI's ability to detect plaque inflammation.
Spio mri studies by dr naghavi
1) The document discusses a study using magnetic resonance imaging (MRI) to detect active atherosclerotic plaque in mice after injection of superparamagnetic iron oxide (SPIO) nanoparticles.
2) The study found that SPIO nanoparticles were taken up by macrophages within atherosclerotic plaques, darkening the plaques on post-injection MRI scans.
3) Histological analysis confirmed iron particle presence within plaques that correlated with macrophage density, supporting MRI's ability to detect plaque inflammation.
Acc presentation macrophage (1)
This document proposes a non-invasive method using SPIO (super paramagnetic iron oxide) nanoparticles to image macrophage infiltration and inflammation in vulnerable atherosclerotic plaques. Rabbits and mice were injected with SPIO, which accumulated in inflamed plaque areas correlated with macrophage density. SPIO-enhanced MRI then successfully identified these inflamed plaques non-invasively in vivo. This technique could provide a way to detect rupture-prone plaques and better understand plaque vulnerability.
051 plaque inflam detection in atherosclerotic rabbits
This document proposes a non-invasive method using SPIO (super paramagnetic iron oxide) nanoparticles to image macrophage infiltration and inflammation in vulnerable atherosclerotic plaques. Rabbits and mice were injected with SPIO, which accumulated in macrophage-rich areas of plaques. MR imaging found SPIO accumulation correlated with macrophage density in plaques. Ex-vivo studies of rabbit aortas also demonstrated SPIO accumulation in inflamed plaques. The authors conclude SPIO-enhanced MRI can non-invasively identify inflamed, vulnerable plaques prone to rupture.
Acc presentation (1)
This document describes a study that introduces a non-invasive method for imaging macrophage infiltration in inflamed atherosclerotic plaques using superparamagnetic iron oxide (SPIO) nanoparticles and MRI. The researchers injected SPIO into hypercholesterolemic and normal rabbits and found that SPIO profoundly accumulated in areas of macrophage infiltration in the atherosclerotic plaques, as confirmed by histology. SPIO-enhanced MRI was able to identify these inflamed plaques non-invasively. The results suggest SPIO-enhanced MRI can be a novel method for detecting rupture-prone inflamed plaques associated with heart attacks and strokes.
Acc presentation (1)
This document discusses a study evaluating the use of superparamagnetic iron oxide (SPIO) nanoparticles to non-invasively image macrophage infiltration in atherosclerotic plaques via MRI. Researchers injected SPIO into hypercholesterolemic and normal rabbits and found SPIO accumulation correlated with macrophage density in plaques, as observed via histology. SPIO-enhanced MRI identified inflamed plaques in hypercholesterolemic rabbits compared to normal rabbits. The study demonstrates SPIO nanoparticles can be used to non-invasively detect active inflammation in vulnerable atherosclerotic plaques.
Acc presentation lipid coated(2)
This document discusses a novel tracer for MRI imaging of macrophage infiltration in atherosclerotic plaque. It summarizes research into lipid-coated superparamagnetic iron oxide nanoparticles (SPIOs) that are phagocytosed by macrophages. The researchers tested various SPIO coatings and sizes to maximize macrophage uptake while minimizing oxidative stress. Lipid-coated SPIOs combined with certain aminoglycans showed the highest uptake and lowest induction of reactive oxygen species. The goal is to develop an MRI contrast agent that can noninvasively image vulnerable, inflamed plaques by detecting macrophage presence.
Spio mri studies by dr naghavi - presented to ge-amersham
Spio mri studies by dr naghavi - presented to ge-amershamSociety for Heart Attack Prevention and Eradication
This document discusses non-invasive imaging of vulnerable plaque using macrophage targeting contrast agents such as superparamagnetic iron oxide (SPIO) nanoparticles. SPIO nanoparticles are engulfed by macrophages and accumulate in inflamed plaques, shortening MRI relaxation times and allowing identification of active plaques. Studies in animal models demonstrate the feasibility of SPIO-enhanced MRI for detecting vulnerable plaque. However, limitations include the need to balance morphology and activity imaging, as well as poor resolution for coronary applications and the cost and complexity of SPIO injection and delayed imaging.Spio mri studies by dr naghavi-presented to ge-amersham
Spio mri studies by dr naghavi-presented to ge-amershamSociety for Heart Attack Prevention and Eradication
This document discusses non-invasive imaging of vulnerable plaque using macrophage imaging with targeted contrast agents such as superparamagnetic iron oxide (SPIO) nanoparticles. SPIO nanoparticles are engulfed by macrophages and accumulate in inflamed plaques, shortening MRI relaxation times and allowing identification of active versus inactive plaques. Studies in animal models demonstrate the feasibility of SPIO-enhanced MRI for detecting vulnerable plaques. However, limitations include the need for further improvements in spatial and temporal resolution for coronary applications as well as the added cost and complexity compared to alternative diagnostic methods.1 spio-mri studies by dr naghavi - amersham oct 2003
This document discusses non-invasive imaging of macrophages for detection of vulnerable plaque using magnetic resonance imaging (MRI) with ultrasmall particles of iron oxide (USPIO). USPIOs are taken up by macrophages and shorten MRI relaxation time, allowing visualization of macrophage activity. Studies show USPIO uptake correlates with macrophage density and plaque inflammation in animal models. While MRI can identify plaque inflammation, limitations include poor resolution for coronary imaging and high cost compared to alternatives.
1 spio-mri studies by dr naghavi - amersham oct 2003
1 spio-mri studies by dr naghavi - amersham oct 2003Society for Heart Attack Prevention and Eradication
This document discusses non-invasive imaging of macrophages for detection of vulnerable plaque using magnetic resonance imaging (MRI) with ultrasmall particles of iron oxide (USPIO). USPIOs are taken up by macrophages and shorten MRI relaxation time, allowing visualization of macrophage activity. Studies show USPIO uptake correlates with macrophage density and plaque inflammation in animal models. While MRI can identify plaque inflammation, limitations include poor resolution for coronary imaging and high cost compared to alternatives.Spio mri studies by dr naghavi - amersham oct 2003 - part 1
Spio mri studies by dr naghavi - amersham oct 2003 - part 1Society for Heart Attack Prevention and Eradication
This document discusses non-invasive imaging of vulnerable plaque using macrophage imaging and superparamagnetic iron oxide (SPIO) contrast agents. It notes that while morphology imaging like IVUS, OCT and MRI can identify inactive plaques, activity imaging is needed to detect inflammation. SPIOs are engulfed by macrophages and can identify active, inflamed plaques on MRI. Studies show SPIOs entering atherosclerotic plaque through macrophages and blood vessels, and are taken up more over time when incubated with macrophages. This allows MRI to detect inflammation and identify vulnerable plaques at risk of rupture.159 spio uptake by macrophages
This document describes a study comparing four types of superparamagnetic iron oxide (SPIO) nanoparticles and their ability to be taken up by macrophages and detect plaque inflammation via MRI. The study finds that lipid-coated SPIO-2 is taken up strongly by macrophages, induces less oxidative stress than dextran-coated SPIO, and is not inhibited by a mannose receptor inhibitor. SPIO-2 therefore shows potential as a contrast agent for noninvasive MR imaging of vulnerable atherosclerotic plaques containing macrophage inflammation. Such detection could help identify high-risk patients and reduce deaths from heart attacks and strokes.
159 spio uptake by macrophages
This document describes a study comparing four types of superparamagnetic iron oxide (SPIO) nanoparticles and their ability to be taken up by macrophages and detect plaque inflammation via MRI. The study finds that lipid-coated SPIO-2 is taken up well by macrophages, induces less oxidative stress than dextran-coated SPIO, and is not inhibited by a mannose receptor inhibitor. Lipid-coated SPIO-2 shows potential for contrast-enhanced MRI to detect vulnerable plaques associated with atherosclerosis and acute coronary events. The ability to identify vulnerable plaques through this method could allow for improved treatment and reduced mortality from heart attacks and strokes.
Ea slipidcoated final july 2
This document describes a study comparing four types of superparamagnetic iron oxide (SPIO) nanoparticles and their ability to be taken up by macrophages and detect plaque inflammation via MRI. The study found that lipid-coated SPIO-2 was taken up strongly by macrophages with less oxidative stress than dextran-coated SPIO and was not inhibited by a mannose receptor inhibitor. MRI of ApoE-deficient mice detected decreased aortic wall signal and irregularities after SPIO injection compared to wild-type mice, indicating lipid-coated SPIO may be a promising candidate for contrast-enhanced MRI detection of vulnerable plaques.
Similar to 053 histologic evidence of spio accumulation in vulnerable plaques (20)
051 plaque inflam detection in atherosclerotic rabbits
051 plaque inflam detection in atherosclerotic rabbits
Spio mri studies by dr naghavi - presented to ge-amersham
Spio mri studies by dr naghavi - presented to ge-amersham
Spio mri studies by dr naghavi-presented to ge-amersham
Spio mri studies by dr naghavi-presented to ge-amersham
1 spio-mri studies by dr naghavi - amersham oct 2003
1 spio-mri studies by dr naghavi - amersham oct 2003
1 spio-mri studies by dr naghavi - amersham oct 2003
1 spio-mri studies by dr naghavi - amersham oct 2003
Spio mri studies by dr naghavi - amersham oct 2003 - part 1
Spio mri studies by dr naghavi - amersham oct 2003 - part 1
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This document discusses developing an artificial intelligence system to predict short-term cardiovascular disease (CVD) events. The goal is to eradicate unexpected heart attacks by predicting risk similar to hurricane forecasts. Existing studies are cited that show over 50% of heart attacks are first symptoms of underlying disease. The document outlines previous work by SHAPE to define vulnerable patients and release guidelines. It proposes using machine learning on existing cohort data to develop algorithms predicting heart attacks within 12 months, and validate the system. The hope is this can trigger preventative actions and add over 10 years to life expectancy. Funding is needed to implement the proposed "Machine Learning Vulnerable Patient Project".AHA SHAPE Symposium 2017 Dr. Kloner Presentation
Triggers of cardiovascular events can include physical and emotional stress. Stress from events like earthquakes, blizzards, intense sporting games, and overexertion from activities like snow shoveling have been shown to increase the risk of acute cardiovascular outcomes like myocardial infarction. While modern therapies have improved cardiovascular health, research continues to show temporary increases in cardiovascular mortality associated with highly emotional sporting events even in recent years. Managing risk factors, reducing stress, and utilizing preventative therapies may help reduce the impact of triggers on cardiovascular health.
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The document introduces the All of Us Research Program, which aims to collect health data from one million Americans to advance precision medicine research. It was announced by President Obama in 2015. The program receives funding from the federal government and private partners. It collects various types of health data from participants through surveys, health records, samples, and devices. The data is stored and shared securely while protecting privacy. The goal is to generate new medical discoveries and more personalized healthcare through collaboration between researchers and participants.AHA SHAPE Symposium 2017 Dr. Yen Presentation
A machine learning model outperformed the ACC/AHA Pooled Cohort Equations Risk Calculator in detecting high-risk asymptomatic individuals and recommending statin treatment for cardiovascular disease prevention in the Multi-Ethnic Study of Atherosclerosis. The machine learning model used support vector machines and data augmentation to derive a CVD risk predictor from nine variables in the MESA study population. It demonstrated higher sensitivity, specificity, and AUC compared to the ACC/AHA risk calculator, recommending statin treatment for fewer individuals while missing fewer cardiovascular events.
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Vu lplaque1 pasterkamp
This document summarizes a post-mortem study examining the prevalence of inflammatory cells in non-ruptured atherosclerotic plaques. The study found that moderate or heavy staining for macrophages was present in 45% of femoral artery cross-sections and 84% of femoral arteries had at least one cross-section with moderate/heavy inflammation. There was no observed relationship between the degree of inflammation in the left and right coronary arteries within individuals, indicating the level of local inflammation is locally determined with little predictive value for other arteries.
Vulnerable plaque vulnerable patient
The document provides guidelines for defining vulnerable plaque and vulnerable patients from the Association for Eradication of Heart Attack. It outlines major and minor histopathological and clinical criteria for vulnerable plaque including active inflammation, thin fibrous cap with large lipid core, endothelial denudation, and stenosis. Potential screening and diagnostic methods are discussed at the plaque, systemic, and blood levels ranging from non-invasive imaging to intravascular techniques. Different types of vulnerable plaque that can cause acute coronary events are also categorized.
Vulnerable plaque overview
Vulnerable plaque refers to dangerous forms of atherosclerotic plaques that can rupture or induce thrombosis, disrupting blood flow. The document discusses the history and research around vulnerable plaque, including pioneers in the field and emerging techniques to detect vulnerable plaque such as intravascular ultrasound, optical coherence tomography, and magnetic resonance imaging. It summarizes that vulnerable plaques are typically characterized by a thin fibrous cap, large lipid core, and presence of macrophages.
Vulnerable patient talk in poland
The document summarizes research on vulnerable plaques and markers of vulnerability. It finds that ruptured plaques are the most common type of culprit lesion, accounting for around 70% of cases. Major criteria for defining vulnerable plaque include outward remodeling, endothelial dysfunction, and a thin fibrous cap with a large lipid core. Both plaque morphology and activity need to be assessed to identify vulnerability.
Vulnerable patient slides without movies
This document contains a summary of a presentation on vulnerable patient syndrome. It includes PowerPoint slides and videos on defining and identifying vulnerable plaques and patients. It thanks sponsors for their support of the educational event. The slides define vulnerable plaques as those likely to rupture in the future, causing heart attacks, and provide criteria for identifying them based on morphology and activity. Biomarkers and conditions that increase plaque and myocardial vulnerability are also summarized. The presentation outlines a pyramid approach for screening, diagnosing, and treating vulnerable patients annually to help reduce heart attacks and their high costs.
Vulnerable patient mar 04
This document discusses triggers for sudden cardiac arrest (SCA) and death (SCD). It notes that over 2/3 of SCD cases are unable to be predicted due to a lack of well-established risk factors. While population risk factors can identify at-risk groups, they cannot predict risk for individuals. The document explores various biological, anatomical, and environmental factors that can precipitate fatal arrhythmias and discusses how the timing of transient initiating events is critical for the development of ventricular tachyarrhythmias. It emphasizes that myocardial electrophysiological processes likely determine the onset or lack of VT/VF/SCD and that immediate access to automated external defibrillators is needed to save lives.
Vulnerable (thrombogenic blood
This document summarizes presentations from symposia on vulnerable plaque and discusses the relationship between plaque, blood, and patients in atherothrombosis. It notes that multiple factors like diabetes, smoking, and hyperlipidemia can make blood more thrombogenic and moderate the severity of acute events after plaque rupture. Statins, aspirin, and other drugs that target tissue factor or thrombin pathways may be promising antithrombotic agents by inhibiting thrombosis initiation and propagation.
Vuln shape aha 2005
The document discusses vulnerable plaque and challenges in detecting and treating it. It describes various imaging techniques for detecting vulnerable plaque such as thermography, MRI, CT angiography, and optical coherence tomography. However, it notes that while these can identify high-risk features, it remains unclear what exactly defines vulnerable plaque and whether imaging findings truly correlate with risk. The document also notes that while statins reduce events, the relationship between plaque burden and events is unclear, and better defining and detecting the disease is still needed before new therapies can be developed.
Vuln plaque poster burke
1) The study examined 92 hearts from patients with severe coronary artery disease who died suddenly. The hearts were sectioned and plaque types were classified.
2) The number of "vulnerable" plaques, particularly thin cap atheromas, was highest in hearts of patients who died from acute plaque rupture and lowest in those with incidental disease.
3) Thin cap atheromas and other unstable plaque types were concentrated in the proximal coronary segments, similar to the distribution of plaque ruptures. The study suggests vulnerable plaques contribute to acute coronary syndromes and are non-uniformly distributed within the coronary arteries.
Vp watch editorial_slide 25short-
1) Drug-coated stents, particularly those coated with sirolimus, have shown promise in reducing restenosis compared to bare metal stents. Sirolimus inhibits cell proliferation and has been shown in studies to reduce intimal hyperplasia and restenosis in animal models by 50% or more.
2) A study by Suzuki et al. found that a sirolimus-coated stent reduced restenosis by 50% through inhibiting cellular proliferation in a dose-dependent manner compared to a bare metal stent. Adding dexamethasone to the coating did not provide additional benefit.
3) If results of the RAVEL clinical trial showing "zero" restenosis out to 5 years
Vp watch editorial_slide25
This document discusses drug-coated stents for preventing restenosis. It summarizes a study showing that stents coated with sirolimus via a polymer matrix reduced restenosis by 50% by inhibiting cell proliferation. Adding dexamethasone provided no additional benefit. Other studies also showed sirolimus inhibits smooth muscle cell proliferation. If results of the RAVEL trial showing "zero" restenosis at 210 days hold true long-term, sirolimus-coated stents may become the standard therapy for coronary revascularization. Questions are raised about whether coating vulnerable plaques could be a primary treatment and if multiple vulnerable plaques would all be stented.
Vp watch editorial_slide25short-
1) Drug-coated stents, particularly those coated with sirolimus, have shown promise in reducing restenosis compared to bare metal stents. Sirolimus inhibits cell proliferation and has been shown in studies to reduce intimal hyperplasia and restenosis in animal models by 50% or more.
2) A study by Suzuki et al. found that a sirolimus-coated stent reduced restenosis by 50% through inhibiting cellular proliferation in a dose-dependent manner compared to a bare metal stent. Adding dexamethasone to the coating did not provide additional benefit.
3) If results of the RAVEL clinical trial showing "zero" restenosis out to 5 years
Vp symposium31602
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Vpschoenhagen
This study used intravascular ultrasound to examine arterial remodeling and plaque characteristics in 131 patients with either stable angina or recent unstable symptoms. Patients with unstable presentations had greater plaque burden at the culprit lesion despite similar luminal narrowing, and a greater extent of positive arterial remodeling compared to those with stable angina. The culprit lesions in unstable patients also showed a higher rate of echolucent plaque morphology. This suggests that bulky, remodeled plaques may be more vulnerable to rupture, leading to acute coronary syndromes. Further prospective study is needed to better understand the relationship between clinical presentation and plaque features.
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The document discusses ways to improve prediction of coronary events beyond the traditional Framingham Risk Score (FRS). It finds that adding C-reactive protein (CRP) measurement to the FRS results in a better model that significantly improves risk prediction, especially for those at intermediate risk. Measuring coronary artery calcium via scanning also seems to enhance prediction over the FRS alone. However, these findings require replication in other patient populations before firmly concluding CRP and calcium scoring can modify physician interpretation of patient risk based on the FRS.
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053 histologic evidence of spio accumulation in vulnerable plaques
- 1. Histologic evidence ofHistologic evidence of SPIO accumulation inSPIO accumulation in vulnerable plaquesvulnerable plaques Silvio Litovsky, MDSilvio Litovsky, MD June 7, 2002June 7, 2002
- 2. In-vitro Study of Macrophage SPIO Uptake In a series of in-vitro studies we have tested the rate of SPIO uptake by human activated monocytes in different conditions regarding incubation time and concentration of SPIO. SPIO particles were labeled by a fluorescent dye (DCFA).
- 3. FL-labeled SPIO Incubated Macrophages after 24hr
- 4. Double DAPI Staining with Fluorescence-labeled SPIO Macrophages after 24hr Incubation
- 5. Macrophages labeled with fluorescent microspheres adhere to atherosclerotic plaques.
- 6. In-vivo distribution of SPIO in ApoE deficient and wild type mice: •For the initial study, we use the mouse model of atherosclerosis. •ApoE deficient mouse has similar atherosclerotic lesions to human and the lesions are more common in the aortic arch and thoracic aorta. • We used ApoE deficient mice and normal variant (C57BL mice) as control. •Animals were sacrificed on day 3 and 5 after injection.
- 7. •Tissues from different organs including liver, kidneys, lung, heart, spleen and aorta (including valve region, ascending, descending and abdominal) were obtained and stained. • We used Hematoxyline and Eosin (H&E), Iron, CD68 and Movat staining. • After Iron staining, aortic walls of the ApoE deficient mice and normal variant were compared based on the number of Iron particles.
- 8. ApoE k/o Mouse 5 Days AfterApoE k/o Mouse 5 Days After Injection- LiverInjection- Liver
- 9. Histopathologic Study of the Mice Injected with SPIO (Aortic Root) ApoE K/O mouse, Movat staining, aortic root Coronary Cross section Atherosclerotic plaque
- 10. Histopathologic study of ApoE K/O Mouse injected With SPIO (Aortic Root) CD68 staining (coronary plaque) Iron Staining (aortic plaque) Iron Staining (coronary section) Iron particles Iron particles
- 11. Histopathologic Study of Wild Type Mouse Injected with SPIO (Thoracic Aorta) H&E staining CD68 stainingIron staining
- 12. Comparison of the Number of the Iron Particles (per HPF) in ApoE K/O Mice Plaque vs. Normal Wall 0 5 10 15 Atherosclerotic Aorta Average number of iron particles per sample P <0.001
- 13. Histopathologic studies of Thoracic aorta in Watanabe Hereditary Hypercholesterolemic rabbit after SPIO injection H&E staining Macrophage staining Iron staining Iron particles
- 14. Hypercholesterolemic Rabbit, Aorta, 4 days after SPIO injection Perls’ Staining H&E Staining X40 X40
- 15. Electron Microscopy Evidence of Intracellular SPIO in the Rabbit Aorta Endothelial cell, x7500 Foamy cell, x4000
- 16. 0 10 20 30 40 50 60 0 10 20 30 40 50 60 70 macrophage (foam cell) density SPIOpositivecell-Iron staining Series1 R=0.956 Correlation Between Iron Positive Cells in Iron Staining and Foam Cell Density in H&E Staining in Rabbit Atherosclerotic Aorta.
- 17. Plaque Iron in ApoE k/o mousePlaque Iron in ApoE k/o mouse after SPIOafter SPIO H&E IRON STAIN
- 18. Plaque Iron in Cytokine-TreatedPlaque Iron in Cytokine-Treated Mice after SPIOMice after SPIO H&E stain Iron stain
- 19. Plaque Iron in Cytokine–Treated MicePlaque Iron in Cytokine–Treated Mice after SPIOafter SPIO Overlying plaques Plaque-free area
- 20. Conclusion: 1) SPIO nanoparticles actively accumulate in superficial areas of atherosclerotic lesions in rabbits and mice. 2) There is a strong correlation between areas of foamy cells infiltration and SPIO uptake in mice and rabbit plaques. 3) Cytokines markedly increase the SPIO uptake by atherosclerotic plaques, thus providing a method for quantifying recruitment of monocytes. 4) MRI has the potential for non-invasively assessing monocyte homing into atherosclerotic plaque.
- 21. Center for Vulnerable Plaque ResearchCenter for Vulnerable Plaque Research Houston, TexasHouston, Texas