It's a general topic: relationship of the Hawaiian bobtail squid and Vibrio fischeri bacteria which is very common light prodcing microorganism. It explains how the symbiosis relationship of these two very different organisms work and how they help each other in their survival. Also, how this concept of light producing bacteria in living form can be used for different microbial techniques and identification of certain chemicals; and can be used on platform to built a business related to microbiology or biotechnology industries.

1. THE BEAUTIFUL RELATIONSHIP
The Hawaiian bobtail squid and its light producing Bacteria(Vibrio fischeri )

3. CONTENT
• What do beautiful relation actually means.
• How do the squid and v.fischeri manage their relationship. And why?
• Vibrio fischeri bacterial characteristics
• Bioluminescenes (why and How)
• Benefits ( food industry, lightning, biomedical imaging, etc, etc)
• Inference and CONCLUSION.

4. • Symbiotic relationship
• Squid give cozy place to live, sugar and protein for their growth.
• Vibrio protect the squid

5. DID I JUST SAY PROTECT!

6. HOW CAN A TINY BACTERIA
PROTECT A SQUID?
• it produce the right light to blend in with the moonlight so that squid can cruise the
night-time undetected by the predator and hunt for his food(shrimps).
• Camouflage

7. TO ATTRACT PREY
Angler fish

8. VIBRIO FISCHERI
• Gram negative , rod shaped , proteobacter.
• The bacteria produce the chemical signal named ‘ autoinducer’, when it’s in
confined space i.e., light organ of squid , amount of autoinducers starts to build up
and once the critical concentration is reached; it triggers the bacteria to switch on
the gene for producing light.
• Quarum sensing :the chemical form of communication.

10. HOW THEY ENTER THE SQUID’S
BODY

11. HOW WILL THIS HELP US?

12. BIOLUMINESCENCE IN SEA

13. WHEN YOU LOOK AT SOME
SOURCES OF LIGHT INDUSTRY
Lighting consumes a substantial amoun
of electricity.

14. SO IT MEANS,
• Incandescence is Hot light / Black body radiation,
• Creates light by heating of filament.
• Highly inefficient! 90% of energy is wasted to heat and UV.
• Adverse impact on environment
• Short life span(1000-1500hr)
• Susceptibility to shocks and vibrations.

15. BUT BIOLUMINESCENCE AS
SOLUTION
• Cold light
• Most efficient (all light source by 98% efficiency)
• As cultured as micro-organic level, immune to external
impacts
• No environmental impacts ( no use of coal or other
sources to generate light)
• Unlimited source
• Grow your own light!

16. BIO-LUMINESCENCE IMAGING
CONCEPT
Bacterial cell is
bioluminescent
labeled
Labeled gene is
implanted into host
to multiply
Host is placed in
highly sensitive CCD
camera for 3D image
A spectral analysis is
used to see the
progress of
malignant cell

17. COMPARE IT WITH OTHER TECHNIQUES

18. ESTIMATING METHOD FOR DETECTION
OF FOOD CONTAMINATION
• Standard plate count
Expensive
Time consuming- long wait involved
Labor intensive
No immediate confirmation for consumption
Need specialize transportation

19. ATP BIOLUMINESCENCE IN FOOD
INDUSTRY
This visible glow of pathogen helps provide instant counting results.

20. CREATION OF DYNAMIC ART
BIOGLUPHS project
The center for biofilm Engineering and the Montana State University School of Art and Science

21. INFERENCE AND CONCLUSION
• Change provides opportunity to do something really new with lots of benefits.
• Bioluminescence provides a new dimension to lightning, Healthcare and Food.
• Adoption of this technology will lead to massive growth Bioluminescence.

22. OTHER THAN THIS
• You can buy a story book “The Squid, the Vibrio & the Moon” for better
understanding of this Topic. Created by Dr. Gregory Crocetti