RNA interference [ RNAi] is a sequence mechanism , triggered by the introduction of ds RNA leading to mRNA degradation
It results in switching the targeted gene on and off at transcriptional or post transcriptional level3
The long double stranded RNAs enter a cellular pathway that is known as RNA interference pathway .
First ds RNAs get processed into 20-25 nucleotides small interfering RNAs [siRNAs] by an enzyme Dicer .
Small interfering RNAs assemble into RNA induced silencing complexes [RISCs] ,unwinding in the process.
The siRNAs strands subsequently guide the RISCs complementary RNA molecules , where they cleave and destroy the RNA .
Cleavage of RNA takes place near the middle of the region bound by siRNA strand .
This results into mRNA Degradation.
Gene knockdown
Double stranded RNA is synthesized with a sequence complementary to a gene of interest and introduced into a cell organism ,where it is recognized as exogenous genetic material and activates the RNAi pathway .
Using this mechanism, researchers can cause drastic decrease in the expresssion of targeted gene .
Since RNAi may not totally abolish expression of the gene , this technique is referred to as knockdown.
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RNA – i PATHWAY
1. RNA – i PATHWAY
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2. RNAi INTERFERENCE
RNA interference [ RNAi] is a sequence mechanism , triggered
by the introduction of ds RNA leading to mRNA degradation
It results in switching the targeted gene on and off at
transcriptional or post transriptional level
3. Mechanism
The long double stranded RNAs enter a cellular pathway
that is known as RNA interference pathway .
First ds RNAs get processed into 20-25 nucleotides small
interfering RNAs [siRNAs] by an enzyme Dicer .
Small interfering RNAs assemble into RNA induced silencing
complexes [RISCs] ,unwinding in the process.
4. Continued…..
The siRNAs strands subsequently guide the RISCs
complementary RNA molecules , where they cleave and destroy
the RNA .
Cleavage of RNA takes place near the middle of the region
bound by siRNA strand .
This results into mRNA Degradation.
5.
6.
7. Applications ..
Gene knockdown
Pathway analysis
Treatment of cancer
In biotechnology
Detection of biomarkers
Food products
Crops
8. Gene knockdown
Double stranded RNA is synthesized with a sequence
complementary to a gene of interest and introduced into a
cell organism ,where it is recognized as exogenous genetic
material and activates the RNAi pathway .
Using this mechanism, researchers can cause drastic
decrease in the expresssion of targeted gene .
Since RNAi may not totally abolish expression of the gene ,
this technique is referred to as knockdown.
![RNAi INTERFERENCE
RNA interference [ RNAi] is a sequence mechanism , triggered
by the introduction of ds RNA leading to mRNA degradation
It results in switching the targeted gene on and off at
transcriptional or post transriptional level](data:image/gif;base64,R0lGODlhAQABAIAAAAAAAP///yH5BAEAAAAALAAAAAABAAEAAAIBRAA7)