This free webinar hosted by Scintica Instrumentation provided a comprehensive review of the latest laser confocal microscope technology called the FIVE2 from Optiscan Imaging. The session included a hands-on demonstration of the system and an overview of various preclinical applications supported by sample images from a variety of animal models and tissue types.
18. • Standard Diameter: 3.8mm
• (1) Short Probe:
46mm Rigid Length
• (2) Standard Probe:
66mm Rigid Length
• (3) Laparoscopic Probe:
300mm Rigid Length
1
2
3
4mm diameter probe
placed in gentle contact
with tissue being imaged.
MINIATURISED SCANNER CONFIGURATION
Contact site is counted as Z = 0
19. 19
• The confocal endomicroscope is an
“optical sectioning” device
(Out of focus material turns black, instead of blurry)
• It isolates A thin plane of cells, viewed en face
(en face = parallel to tissue surface, unlike typical cut sections)
• One of the most important user operations
is the control of the imaging plane depth
• Imaging depth up to 400um
• Tissue dependent
THE ‘Z’ DIMENSION
Capturing 3D Volumes and Enabling 3D Visualization
X Axis Y Axis
Z Axis
22. Image does not show included
3-way footswitch, or optional items
Confocal Processor
PC, Monitor, Keyboard
and Mouse
Probe
Animal Stage
and Probe
Holder
Imager Application
+ Fiji (ImageJ)
FIVE2 Components
27. VIEWNVIVO DETECTION FILTERS
Filte r Wh e e l Con ce pt Illu stration
*EMPTY Filter Positions Can Be Used
For ‘Custom Filters’ Which May Be
Specified And Purchased, At An
Additional Cost, At The Time Of
Purchasing The Viewnvivo System
Filter colours and location on the filter wheel are used for illustration purposes only and do not represent the actual filter colour or the detection properties
36. Courtesy of Dr Philip Currie
Cancer Fibrous Tissue Normal Tissue
FIVE2 Images:
Instant, easy, live
and in vivo!
Classical Histology:
Tedious prep, slow,
possible artifacts
and ex vivo!
37. Images courtesy of: Martin Goetz and Ralf Kiesslich, Mainz University Hospital, Germany
MOUSE BRAIN MICROVASCULAR IMAGING & CELL TRACKING
38. DOG STOMACH
Image courtesy of Researchers at the University of Melbourne, Faculty of Veterinary and Agricultural Sciences
39. Mitotic cell
nucleus
Ejecting epithelial cell
Brush border &
mucus layer
Images courtesy of Cameron Nowell and researchers at Monash University, Melbourne, Australia
40.
41. TUMOUR ANGIOGENESIS IN VIVO
Images courtesy of: Dr Liem Vo & Peter Anikijenko, Monash University, Melbourne, Australia.
Normal Murine
Dermal Microvasculature
Melanoma-Affected
Dermal Microvasculature
42. TISSUE ENGINEERING - CARTILAGE
Chondrocytes
Contrast Agent:
Fluorescein Sodium
0.5% in PBS; pH 7.4
fov=500µm
fov=~100µm
Images courtesy of Curtain
University, Perth Western
Australia
Wu JP, Kirk TB, Zheng MH. Study of the collagen structure in the superficial zone and
physiological state of articular cartilage using a 3D confocal imaging technique.
J Orpthop Surg 2008; 3: 29.
43. SHEEP CARTILAGE DEFECT MODEL
Condyle
Contrast Agent:
Acriflavine 0.05%
Defect area
Normal
condyle
Defect area
post ACI
Normal
condyle
Wu JP, Kirk TB, Zheng MH. Study
of the collagen structure in the
superficial zone and
physiological state of articular
cartilage using a 3D confocal
imaging technique.
J Orpthop Surg 2008; 3: 29.
fov=500µm fov=500µm
44. IN VIVO RECTAL MUCOSAL BARRIER FUNCTION
Gracie Vargas, Kathleen Listiak Vincent, Yong Zhu, David Szafron, Tyra Caitlin Brown, Paula Patricia Villarreal, Nigel Bourne, Gregg N. Milligan, Massoud Motamedi. In Vivo Rectal
Mucosal Barrier Function Imaging in a Large-Animal Model by Using Confocal Endomicroscopy: Implications for Injury Assessment and Use in HIV Prevention Studies. ASM 2016; 60
57. Bundle Fiber
No cellular details!
FIVE2
Subcellular resolution!
APPLICATIONS OF FLUORESCENCE IN VIVO ENDOMICROSCOPY
Cancer Vasculature Gastrointestinal Skeletal Skin
58. If you have any questions related to FIVE2
technology, please reach out to our product
manager.
Mohammedayaz Rangrez
MSc, PhD
Product Manager
Scintica Instrumentation
Phone: +1 (519) 914 5495
mrangrez@scintica.com
THANK YOU!