Provide a one-sentence definition for horizontal gene transfer. What is the significance of horizontal gene transfer for prokaryotic genetic diversity and classification? How is horizontal gene transfer accomplished? Solution Answer: (A) Horizontal gene tansfer: It is a process through which genetic material are transferred between bacteria that are not parents and offspring. (b) Through horizontal gene transfer, a substantial amount of genetic material can be inserted or deleted from genomes of prokaryotic organisms. Insertion of new trait from one distant related organism to other organism through horizontal gene transfer can lead to new pathogenic and ecological character thus causing microbial diversification. (c) Horizontal gene transfer is accomplished by three main mechanism: Transformation:Genetic material of an individual cell is altered by the direct incorporation of foreign (exogenous) DNA into its genome. Transduction: This process involves transfer of DNA from one bacterium into another through bacteriophages. Conjugation: Conjugation required cell to cell contact or any sexual pilus for transfer of DNA..