How to get pure culture from mixed sample How to get pure culture from mixed sample Solution ANSWER: The two major steps involved in obtaining pure culture from a mixed population. 1)First, take the mixture and make them to dilute until the various micro organisms individuals become separated far.After incubation on the agar plate form visible colonies isolated from other microorganisms.this is formed on isolated plate. 2)Then an isolated colonies can be picked off from isolated plate, and transferred to a new sterile medium. After incubation all organisms in the new culture will be descendants of the same organism, that forms a pure culture......