What are the major uses for artificially synthesized DNA? a) They are used for DNA sequencing, site-directed mutagenesis, but cannot be used as nucleic acid hybridization probes for detection of specific DNA or RNA in the samples. b) They are used as primers for DNA sequencing, site-directed mutagenesis, and as nucleic acid hybridization probes for detection of specific DNA or RNA in the samples. c) They are used as primers for DNA sequencing and site-directed mutagenesis only. a) They are used for DNA sequencing, site-directed mutagenesis, but cannot be used as nucleic acid hybridization probes for detection of specific DNA or RNA in the samples. b) They are used as primers for DNA sequencing, site-directed mutagenesis, and as nucleic acid hybridization probes for detection of specific DNA or RNA in the samples. c) They are used as primers for DNA sequencing and site-directed mutagenesis only. Solution Artificially synthesized DNA in site-directed mutagenesis: The basic procedure for site-directed mutagenesis requires the synthesis of a short DNA primer, an artificially synthesized DNA. This synthetic primer contains the desired mutation and is complementary to the template DNA around the mutation site so it can hybridize with the DNA in the gene of interest. The mutation may be a single base change (a point mutation), multiple base changes, deletion, or insertion. The single-strand primer is then extended using a DNA polymerase, which copies the rest of the gene. The gene thus copied contains the mutated site, and is then introduced into a host cell as a vector and cloned. Finally, mutants are selected by DNA sequencing to check that they contain the desired mutation. Artificially synthesized DNA as primers for DNA sequencing and as nucleic acid hybridization probes for detection of specific DNA or RNA in the samples: DNA sequencing can be done by hybridization method that uses a DNA microarray. Unlike proteins, which have diverse sizes and charges, there is no easy way separate out a specific sequence of DNA based on its general physical and chemical properties. You cannot chemically stain for a specific sequence of DNA. In contrast, it is often possible to use a specific stain to detect a specific protein. Once a DNA sequence is known, the complementary strand can be synthesized and used to detect the strand of interest in a sample. A single pool of DNA whose sequence is to be determined is fluorescently labeled and hybridized to an array containing known sequences, which are artificially synthesized DNA. Strong hybridization signals from a given spot on the array identifies its sequence in the DNA being sequenced. This method of sequencing utilizes binding characteristics of a library of short single stranded DNA molecules (oligonucleotides), also called DNA probes, which are artificially synthesized DNA), to reconstruct a target DNA sequence. Non-specific hybrids are removed by washing and the target DNA is eluted. Hybrids are re-arrang.