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Mycoplasma is a genus of bacteria that do not have a cell wall. Would the nigrosin penetrate the
bacteria? Why or why not?
Solution
Answer:
Mycoplasma lack a cell wall around their cell membrane. Since the surface of most bacterial
cells including mycoplasma is negatively charged, the cell surface repels the nigrosin
stain.Nigrosin is an acidic stain and is used as a negative stain. The glass of the slide will stain,
but mycoplasma cells will not.

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